Luteolin and fisetin suppress oxidative stress by modulating sirtuins and forkhead box O3a expression under in vitro diabetic conditions

Arang Kim,Wooje Lee,Jung-Mi Yun 한국영양학회 2017 Nutrition Research and Practice Vol.11 No.5

BACKGROUND/OBJECTIVE: Chronic hyperglycemia induces oxidative stress via accumulation of reactive oxygen species (ROS) and contributes to diabetic complications. Hyperglycemia induces mitochondrial superoxide anion production through the increased activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. This study aimed to determine whether fisetin and luteolin treatments suppress the oxidative stress by modulating the expression of sirtuins (SIRTs) and forkhead box O3a (FOXO3a) under hyperglycemic conditions in human monocytes. MATERIALS/METHODS: Human monocytic cells (THP-1) were cultured under osmotic control (14.5 mmol/L mannitol), normoglycemic (NG, 5.5 mmol/L glucose), or hyperglycemic (HG, 20 mmol/L glucose) conditions, in the absence or presence of fisetin and luteolin for 48 h. To determine the effect of fisetin and luteolin treatments on high glucose-induced oxidative stress, western blotting and intracellular staining were performed. RESULTS: Hyperglycemic conditions increased the ROS production, as compared to normoglycemic condition. However, fisetin and luteolin treatments inhibited ROS production under hyperglycemia. To obtain further insight into ROS production in hyperglycemic conditions, evaluation of p47phox expression revealed that fisetin and luteolin treatments inhibited p47phox expression under hyperglycemic conditions. Conversely, the expression levels of SIRT1, SIRT3, SIRT6, and FOXO3a were decreased under high glucose conditions compared to normal glucose conditions, but exposure to fisetin and luteolin induced the expression of SIRT1, SIRT3, SIRT6, and FOXO3a. The above findings suggest that fisetin and luteolin inhibited high glucose-induced ROS production in monocytes through the activation of SIRTs and FOXO3a. CONCLUSIONS: The results of our study supports current researches that state fisetin and luteolin as potential agents for the development of novel strategies for diabetes.

Beta-Catenin Downregulation Contributes to Epidermal Growth Factor-induced Migration and Invasion of MDAMB31 Cells

Arang Kwon,Hyun-Jung Park,Jeong-Hwa Baek 대한구강생물학회 2018 International Journal of Oral Biology Vol.43 No.3

We previously demonstrated that epidermal growth factor (EGF) enhances cell migration and invasion of breast cancer cells in a SMAD ubiquitination regulatory factor 1 (SMURF1)-dependent manner and that SMURF1 induces degradation of β-catenin in CC1 cells. However, the relationship between EGF-induced SMURF1 and β-catenin expression in breast cancer cells remains unclear. So, we investigated if EGF and SMURF1 regulate β-catenin expression in MDAMB31 human breast cancer cells. When MDAMB31 cells were incubated with EGF for 4, 48, and 7 hours, EGF significantly increased expression levels of SMURF1 mRNA and protein while suppressing expression levels of β-catenin mRNA and protein. Overexpression of SMURF1 downregulated β-catenin mRNA and protein, whereas knockdown of SMURF1 increased β-catenin expression and blocked EGF-induced β-catenin downregulation. Knockdown of β-catenin enhanced cell migration and invasion of MDAMB31 cells, while β-catenin overexpression suppressed EGF-induced cell migration and invasion. Furthermore, knockdown of β-catenin enhanced vimentin expression and decreased cytokeratin expression, whereas β -catenin overexpression decreased vimentin expression and increased cytokeratin expression. These results suggest that EGF downregulates β-catenin in a SMURF1-dependent manner and that β-catenin downregulation contributes to EGF-induced cell migration and invasion in MDAMB breast cancer cells.

Zerumbone Suppresses Pro-inflammation and Its Molecular Mechanism Responds to High Glucose and LPS in Differentiated Human Monocyte THP-1 Cells

Arang Kim,Jung-Mi Yun 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Influence of dietary organic trace minerals on enteric methane emissions and rumen microbiota of heat-stressed dairy steers

Arang Son,Mahfuzul Islam,Seon-Ho Kim,이성실,이성실 한국축산학회 2023 한국축산학회지 Vol.65 No.1

Ruminants are the main contributors to methane (CH4), a greenhouse gas emitted by livestock, which leads to global warming. In addition, animals experience heat stress (HS) when exposed to high ambient temperatures. Organic trace minerals are commonly used to prevent the adverse effects of HS in ruminants; however, little is known about the role of these minerals in reducing enteric methane emissions. Hence, this study aimed to investigate the influence of dietary organic trace minerals on rumen fermentation characteristics, enteric methane emissions, and the composition of rumen bacteria and methanogens in heat-stressed dairy steers. Holstein (n=3) and Jersey (n=3) steers were kept separately within a 3×3 Latin square design, and the animals were exposed to HS conditions (Temperature-Humidity Index [THI], 82.79 ± 1.10). For each experiment, the treatments included a Control (Con) consisting of only basal total mixed rations (TMR), National Research Council (NRC) recommended mineral supplementation group (NM; TMR + [Se 0.1 ppm + Zn 30 ppm + Cu 10 ppm]/kg dry matter), and higher concentration of mineral supplementation group (HM; basal TMR + [Se 3.5 ppm + Zn 350 ppm + Cu 28 ppm]/kg dry matter). Higher concentrations of trace mineral supplementation had no influence on methane emissions and rumen bacterial and methanogen communities regardless of breed (p > 0.05). Holstein steers had higher ruminal pH and lower total volatile fatty acid (VFA) concentrations than Jersey steers (p < 0.05). Methane production (g/d) and yield (g/kg dry matter intake) were higher in Jersey steers than in Holstein steers (p < 0.05). The relative abundances of Methanosarcina and Methanobrevibacter olleyae were significantly higher in Holstein steers than in Jersey steers (p < 0.05). Overall, dietary organic trace minerals have no influence on enteric methane emissions in heat-stressed dairy steers; however, breed can influence it through selective alteration of the rumen methanogen community.

A Simple GUI-based Sequencing Format Conversion Tool for the Three NGS Platforms

Arang Rhie,이경은,Chin Ting Thong,박현석,양산덕 한국유전체학회 2010 Genomics & informatics Vol.8 No.2

To allow for a quick conversion of the proprietary sequence data from various sequencing platforms, sequence format conversion toolkits are required that can be easily integrated into workflow systems. In this respect, a format conversion tool, as well as quality conversion tool would be the minimum requirements to integrate reads from different platforms. We have developed the Pyrus NGS Sequencing Format Converter, a simple software toolkit which allows to convert three kinds of Next Generation Sequencing reads, into commonly used fasta or fastq formats. The converter modules are all implemented, uniformly, in Java GUI modules that can be integrated in software applications for displaying the data content in the same format.

SMURF1 Plays a role in EGF-induced breast cancer cell migration and invasion

Kwon, Arang,Lee, Hye-Lim,Woo, Kyung Mi,Ryoo, Hyun-Mo,Baek, Jeong-Hwa Springer-Verlag 2013 Molecules and cells Vol.36 No.6

한국 성인 남녀에서 당뇨병 유병률과 혈중 비타민 D 농도와의 관련성 : 제6기 국민건강영양조사(2013∼2014)에 근거하여

김아랑 ( Arang Kim ),윤정미 ( Jung-mi Yun ) 대한영양사협회 2017 대한영양사협회 학술지 Vol.23 No.1

Insufficient vitamin D intake is a major health problem around the world. Recently, many studies have suggested that vitamin D intake may influence insulin resistance. However, little is known about the association between vitamin D and diabetes mellitus. The aim of this study was to investigate the association between serum 25-hydroxy vitamin D (25(OH)D) levels and diabetes mellitus in Korean adults. This study was a cross-sectional analysis of 3,686 participants of the Korean National Health and Nutrition Examination Survey (KNHANES) 2013∼2014 aged 19 years and higher. The results showed that the mean serum 25-hydroxy vitamin D (25(OH)D) level in Korean adults was 16.77 ng/mL, and 74.2% of them had an insufficient serum 25-hydroxy vitamin D (25(OH)D) level (below 20 ng/mL). In normal and pre-diabetic groups, the serum 25-hydroxy vitamin D (25(OH)D) level significantly increased with age (P<0.001), but there was no significant difference relative to age in the diabetic group. Low vitamin D levels (25-hydroxy vitamin D <10 ng/mL) were associated with increased fasting blood glucose levels, compared with optimal vitamin D levels (25-hydroxy vitamin D >30 ng/mL), after adjusting for variables that may affect fasting blood glucose, but this result was not significant. In conclusion, although no significant association was observed between diabetes prevalence and vitamin D levels in this study, further studies are needed because the effect of vitamin D on diabetes remains controversial. This nutrient plays a crucial role in the body, and levels are insufficient in the Korean population.

e-Book 학습자의 학습수용의도에 미치는 요인 분석

오아랑(Arang O),문남미(Nammee Moon),이국세(K.S. Lee) 한국방송·미디어공학회 2011 한국방송공학회 학술발표대회 논문집 Vol.2011 No.11

미디어 디지털컨버전스 환경에서 교육을 수용하는 도구로서 e-Book은 향후 더 많은 확산이 기대되어진다. 본 연구에서는 학습자의 학습도구로서 e-Book과 종이책의 수용 의도를 뇌파를 분석(자기조절지수, 기초율동지수, 주의지수, 활성지수, 정서지수, 항스트레스지수, 좌우뇌균형지수, 브레인지수 분석)하여 실험하였으며, e-Book을 사용 후, 성별에 상관없이 내외적 환경 요인으로 인한 육체적, 정신적 피로도에 대한 저항력을 나타내는 항스트레스 지수가 낮아지는 결과를 얻었다. 향후, 본 연구를 기반으로 개선방향을 찾을 필요가 있다.

Luteolin and fisetin suppress oxidative stress by modulating sirtuins and forkhead box O3a expression under in vitro diabetic conditions

Kim, Arang,Lee, Wooje,Yun, Jung-Mi The Korean Nutrition Society 2017 Nutrition research and practice Vol.11 No.5

BACKGROUND/OBJECTIVE: Chronic hyperglycemia induces oxidative stress via accumulation of reactive oxygen species (ROS) and contributes to diabetic complications. Hyperglycemia induces mitochondrial superoxide anion production through the increased activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. This study aimed to determine whether fisetin and luteolin treatments suppress the oxidative stress by modulating the expression of sirtuins (SIRTs) and forkhead box O3a (FOXO3a) under hyperglycemic conditions in human monocytes. MATERIALS/METHODS: Human monocytic cells (THP-1) were cultured under osmotic control (14.5 mmol/L mannitol), normoglycemic (NG, 5.5 mmol/L glucose), or hyperglycemic (HG, 20 mmol/L glucose) conditions, in the absence or presence of fisetin and luteolin for 48 h. To determine the effect of fisetin and luteolin treatments on high glucose-induced oxidative stress, western blotting and intracellular staining were performed. RESULTS: Hyperglycemic conditions increased the ROS production, as compared to normoglycemic condition. However, fisetin and luteolin treatments inhibited ROS production under hyperglycemia. To obtain further insight into ROS production in hyperglycemic conditions, evaluation of p47phox expression revealed that fisetin and luteolin treatments inhibited p47phox expression under hyperglycemic conditions. Conversely, the expression levels of SIRT1, SIRT3, SIRT6, and FOXO3a were decreased under high glucose conditions compared to normal glucose conditions, but exposure to fisetin and luteolin induced the expression of SIRT1, SIRT3, SIRT6, and FOXO3a. The above findings suggest that fisetin and luteolin inhibited high glucose-induced ROS production in monocytes through the activation of SIRTs and FOXO3a. CONCLUSIONS: The results of our study supports current researches that state fisetin and luteolin as potential agents for the development of novel strategies for diabetes.

XML을 사용한 C언어 소스 코드 분석 및 제어 흐름 분석

정아랑 ( Arang Jeong ),김현수 ( Hyeon Soo Kim ) 한국정보처리학회 2008 한국정보처리학회 학술대회논문집 Vol.15 No.2

제어 흐름 그래프(CFG : Control Flow Graph)는 제어 흐름상의 오류나 문제점을 찾아내고 흐름에 대해 한눈에 파악할 수 있기 때문에 소프트웨어공학 분야에서 많이 사용되고 있다. 현재 여러 테스팅 분야에서 다양한 제어 흐름 분석 기법들이 연구, 소개되고 있는데 본 논문에서는 XML 문서를 이용하여 CFG를 나타내고자 한다. XML은 트리구조를 가진 문서 모델로 C 언어 소스 코드를 구조적으로 나타냄으로써 좀 더 쉽게 코드를 분석하고, 제어 흐름 요소를 추출하여 제어 흐름 그래프를 나타내는 데에 유용하다. 따라서 중간 분석 파일로 XML을 이용하여 보다 빠르고 쉽게 CFG를 나타내는 기법을 제안한다.