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이지호 ( Jiho Lee ),송민호 ( Min-ho Song ),유지우 ( Ji-woo Yu ),이정훈 ( Jeong-hoon Lee ),안희연 ( Hee-yeon Ahn ),금영수 ( Young-soo Keum ) 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-
본 연구는 시설재배 유채, 치커리, 알타리무 내 농약잔류량 감소추이를 확인하여 생산단계 농산물의 농약 잔류허용기준(PHRL: Pre-Harvest Residue Limit) 설정 고시(안) 마련을 위한 기초자료로 활용되어 재배과정에서의 농산물 안전성관리를 통하여 농산물 유통단계 안전관리 강화에 그 목적이 있다. 지리적으로 차이가 있는 2개 시험포장(음성 및 이천)을 선정하여, 유채 중 농약 3종(Indoxacarb, Lufenuron, Pyridalyl), 치커리 중 농약 3종(Fludioxonil, Lufenuron, Pyridalyl), 알타리무 중 농약 4종(Diniconazole, Dinotefuran, Metconazole, Tebuconazole)을 안전사용기준에 따라 살포한 후, 해당 농약의 최종 수확 전 살포일을 고려하여 0, 1, 2, 3, 5, 7 및 10 일차에 걸쳐 시료를 수확하였다. 분석법 확립을 위해 해당 공시료에 농약별 회수율 실험을 실시하였으며, 70∼110%, 변이계수 10% 이하를 만족하였다. 채취한 시료는 확립된 분석법을 바탕으로 전처리 과정을 거쳐 HPLC (HPLC-MS/MS) 및 GC (GC-MS/MS)를 이용하여 정량분석을 수행하였다. 각 시료를 분석하여 일차별 잔류량을 확인하고, 이를 바탕으로 약제별 회귀방정식에 의한 감소상수 및 생물학적 반감기를 산출, 통계학적 방법에 근거한 신뢰성 있는 출하전 생산단계 잔류농약 허용기준(안)을 제시하고자 하였다.
메틸말리올라이드의 NRF2/ARE 유도를 통한 피부 세포 보호 효과
이준 ( June Lee ),김기성 ( Ki Seong Kim ),이현기 ( Hyun Gy Lee ),박창호 ( Changho Park ),구민수 ( Minsu Ku ),금영삼 ( Young-sam Keum ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.4
본 연구진은 메틸말리올라이드가 인간 피부 세포주인 HaCaT 세포에서 NRF2를 유도하고 이를 통하여 항산화 효과를 나타내는지 알아보았다. MTT assay를 통하여 메틸말리올라이드는 10 μ M 농도에서 24 h 동안 HaCaT 세포에 처리하여도 HaCaT 세포 독성을 나타내지 않는 것을 확인하였다. 본 연구진이 구축한 HaCaT-ARE-GFP-luciferase 세포에 메틸말리올라이드를 처리하고 루시퍼라아제 활성을 측정한 결과 메틸말리올라이드는 양성 대조군인 레스베라트롤보다 ARE 결합에 따른 루시퍼라아제 활성을 더욱 강하게 증가시키는 것을 확인하였다. 또한 HaCaT 세포에 메틸말리올라이드 처리 시 NRF2에 의하여 유도되는 항산화 단백질인 HO-1과 NQO1 mRNA 및 단백질 발현이 통계적으로 유의하게 증가하였다. 마지막으로 메틸말리올라이드는 HaCaT 세포에서 TPA에 의해서 유도된 DNA 및 지질의 산화를 강력하게 억제하였다. 결론적으로 본 연구는 메틸말리올라이드가 NRF2 유도를 통하여 피부 산화적 스트레스를 억제하며 이는 메틸말리올라이드가 신규 항산화 화장품의 소재로 적합하다는 것을 시사한다. In the present study, we have investigated whether methylated marliolide could induce NRF2 thereby exerting anti-oxidant effects. MTT assay showed that methylated marliolide did not exhibit cytotoxicity on HaCaT cells. Methylated marliolide induced a higher ARE-dependent luciferase activation in HaCaT ARE-GFP-luciferase cells, compared with resveratrol. In addition, exposure of methylated marliolide to HaCaT cells significantly induced NRF2 and transcriptionally activated HO-1 and NQO1, both of which are target genes of NRF2. Finally, methylated marliolide protected HaCaT cells against TPA-induced oxidative damages on nucleotides and lipids. Together, results shows that methylated marliolide could suppress oxidative damages through induction of NRF2 which implies that methylated marliolide might serve as a good candidate for novel cosmetic ingredient with anti-oxidant effects.
GC-ECD를 이용한 배추, 사과, 감귤, 고추, 현미 중 살균제 Prochloraz의 분석법 확립
이은미 ( Eun Mi Lee ),이혜리 ( Hye Ri Lee ),류명주 ( Myoung Joo Riu ),박희원 ( Hee Won Park ),나예림 ( Ye Rim Na ),송혁환 ( Hyuk Hwan Song ),금영수 ( Young Soo Keum ),김정한 ( Jeong Han Kim ),Young Zhe Zhu 韓國環境農學會 2009 한국환경농학회지 Vol.28 No.4
Prochloraz를 2,4,6-Trichlorophenol (2,4,6-TCP)로 전환시켜 분석하는 방법을 확립하였으며, 배추, 사과, 고추. 감귤, 현미를 대사 농작물로 선정하였다. 농산물 시료를 마쇄, acetonitrile로 추출하여 dichloromethane으로 분배하고 농축액에 pyridine hydrochloride를 넣고 1시간 가열 분해를 한다. 반응액을 다시 dichloromethane으로 분배한 후 GC-ECD로 분석하였다. Prochloraz의 정량한계는 0.01 mg/kg 이었다. 회수율은 0.1 mg/kg 수준에서 105-113%, 0.5 mg/kg 수준에서 82-87% 이었고, 반복 간 분석오차는 10% 미만이었다. 이러한 분석법 개선 과정에서 기존의 soxhlet 추출과 환류장치를 쓰지 않고 용매추출법과 작은 vial을 성공적으로 적용하였고, 간편, 신속, 경제적이며 정밀한 분석법을 확립하였다. Analytical method for prochloraz in cabbage, apple, pepper, mandarin, and hulled rice was established by conversion it to 2,4,6-trichlorophenol (TCP). Crop samples were extracted with acetonitrile, and partitioned with dichloromethane. The sample extracts were hydrolyzed with pyridine hydrochloride in a vial by heating for 1 hour, and analyzed with GC-ECD after partitioning with dichloromethane. Method quantification limit (MQL) of prochloraz was 0.01 mg/kg. Recoveries at 0.1 mg/kg level was 105-113% while at 0.5 mg/kg level was 82-87%. In both of the cases CV was less than 10%. Through this procedure soxhlet extraction and refluxing apparatus of conventional method were discarded and simple solvent extraction and small vial were successfully employed, resulting in simple, rapid, economic and more precise method.
미나리 및 쪽파 중 Fenpropathrin의 잔류 소실양상
이정훈 ( Jeong-hoon Lee ),송민호 ( Min-ho Song ),유지우 ( Ji-woo Yu ),신지원 ( Ji-won Shin ),경기성 ( Kee-sung Kyung ),김태화 ( Tae-hwa Kim ),이지호 ( Jiho Lee ),금영수 ( Young-soo Keum ) 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-
According to the rising interest in health, many people consider the residual pesticides in the crops. However, maximum residue limits (MRLs) of many minor crops are still being established. Therefore, fenpropathrin was selected to establish MRLs in the minor crops; shallot and water dropwort. Fenpropathrin is a pyrethroid insecticide which interferes with the kinetics of sodium channels causing paralysis of insects. In this study, residual characteristics of fenpropathrin in shallot and water dropwort were investigated. The pesticide was applied three times with 7 days intervals. Fenpropathrin in the crops was extracted and purified with the QuEChERS method. The quantitative methods of fenpropathrin were validated in accuracy, linearity, and precision. The limits of quantitation (LOQ) of fenpropathrin in both crops were 0.01 mg/kg. The recovery tests at 3 levels (LOQ, 10 folds of LOQ, and the highest concentration levels of pesticide detected) were in the range of 70 to 120%. The quantification of residual pesticides was performed using a gas chromatography-triple quadrupole mass spectrometer (GC-MS/MS). Fenpropathrin in shallot and water dropwort decreased depending on the days which follow the pseudo-first-order decay curve. The results will give fundamental data for the establishment of MRLs of minor crops.
이정훈 ( Jung-hoon Lee ),안희연 ( Hui-yeon Ahn ),유지우 ( Ji-wooyu ),송민호 ( Min-ho Song ),신지원 ( Ji-won Shin ),박건우 ( Geon-woo Park ),금영수 ( Young-soo Keum ),이지호 ( Ji-ho Lee ) 한국환경농학회 2023 한국환경농학회 학술대회집 Vol.2023 No.0
Chlorate and perchlorate are byproducts of chlorination, which are used for disinfecting food. These chemicals can potentially remain in food during the washing process. Excessive ingestion of chlorate and perchlorate can lead to thyroid disorders and inhibit iodine absorption, resulting in additional health issues. Therefore, it is crucial to establish an analysis method for chlorate and perchlorate in order to set a foundation for future monitoring of these substances in the distributed food. Spinach, a widely consumed leafy green vegetable, contains various compounds such as flavonoids, carotenoids, vitamins, and pigments. As spinach is distributed in various forms within the country, there is a risk of contamination with chlorate and perchlorate during this process. Hence, we focused on spinach, a representative leafy vegetable, to establish an analysis method for chlorate and perchlorate. HPLC-MS/MS was used for quantitative analysis of chlorate and perchlorate in spinach. To analyze chlorate and perchlorate, we developed an analytical method based on the QuPPe (Quick Polar Pesticide extraction) method, a polar compound analysis technique developed by Anastassiades. During the development of the analysis method, the diverse matrix effects of spinach interrupted accurate analysis. By modifying the QuPPe method specifically for spinach analysis, we established the final analysis method.
고등어 및 해조류의 염소산염 및 과염소산염 정량 분석 방법 조사
이정훈 ( Jung-hoon Lee ),안희연 ( Hui-yeon Ahn ),유지우 ( Ji-woo Yu ),송민호 ( Min-ho Song ),신지원 ( Ji-won Shin ),박건우 ( Geon-woo Park ),금영수 ( Young-soo Keum ),이지호 ( Ji-ho Lee ) 한국환경농학회 2023 한국환경농학회 학술대회집 Vol.2023 No.0
Monitoring food contaminants is important to ensure human health. Consumer’s needs for hygiene have increased and led to a consumption of chlorine-based disinfectants and sanitizers. Chlorate and perchlorate, which can be found in washed food, are considered potential contaminants that may have adverse effects on the human body. Therefore, there is an urgent need for analytical methods to assess the toxicity of chlorate and perchlorate. In this study, we aimed to establish and validate analytical methods for the quantification of chlorate and perchlorate in seaweed (as a representative of marine products) and mackerel (as a representative of fish products) to evaluate their contamination levels. Given the substantial worldwide consumption of seafood, it is crucial to ensure the safety of seafood through proper washing processes. It is anticipated that residues of chlorate and perchlorate may be present during these processes. To solve this concern, seaweed and mackerel were selected as target samples based on domestic food intake patterns. The analytical methods were developed based on the QuPPe method (Quick polar pesticide extraction method developed by Anastassiades) with some modifications to the extraction procedure due to the diverse matrix effects. Additionally, for seaweed, which has low moisture content, a final analysis method with an additional rehydration step was established. Quantitative analysis of chlorates and perchlorates was conducted using HPLC-MS/MS, and the establishment and validation of the analytical methods were performed through recovery test.