Anti-diabetic Effect of the Exopolysaccharides (EPS) Produced from Cordyceps sinensis on ob/ob Mice

최장원,Choi, Jang-Won The Korean Society for Biotechnology and Bioengine 2011 KSBB Journal Vol.15 No.5

Anti-diabetic effect of the exopolysaccharides (EPS) produced from submerged mycelial culture of Cordyceps sinensis (Cs) was studiedin a type II diabetic animal model (C57BL/6J ob/ob). This study was designed to determine whether Cs-EPS improves clinical symptoms of type II diabetes in ob/ob mice. After Cs-EPS treatment at doses of 200 mg/kg body weight, the fasting blood glucose levels decreased by 47% after 7 weeks compared with those of the control mice. According to the oral glucose tolerance test, the glucose levels recovered its baseline after 120 min in Cs-EPS-treated mice, although the blood glucose levels increased significantly after 30 min. On the other hand, the control group (not-treated) did not recovered its initial level of glucose after 120 min. Furthermore, food intake, body weight, total plasma cholesterol and triglyceride concentrations in ob/ob mice treated with Cs-EPS were significantly decreased, compared with those in control ob/ob mice. Cs-EPS treatment increased significantly the plasma insulin level and the expression of leptin mRNA in adipose tissue of Cs-EPS-treated ob/ob mice. From these results, it is demonstrated that Cs-EPS could be effective for regulating normal blood glucose levels by increasing the amounts of plasma insulin and leptin expression in ob/ob mice, indicating that this compound could be a candidate material as a dietary supplement to control hyperglycemia in patients suffering from type II diabetes.

융합의 시대에(사물인터넷시대에)한국 노인의 장기요양 서비스 이용 상태 전환과 결정요인 분석

최장원,Choi, Jang-Won 한국사물인터넷학회 2020 한국사물인터넷학회 논문지 Vol.6 No.4

본 연구는 한국 노인장기요양 서비스 이용 상태의 결정요인과 상태 의존성을 파악하고자 하였다. 이를 위해 한국복지패널 자료를 이용하여 시간이 지남에 따른 서비스 이용 상태간 전환 패턴을 랜덤효과 다항로짓 모형을 이용하여 분석하였다. 그 결과 노인장기요양 서비스 이용 상태에 있어 강한 상태 의존성을 확인하였다. 특히 초기 상태에서 노인장기요양보험 이용자는 상태가 지속되는 경향이 강한 것으로 나타났다. 개인의 인구통계학적 특성 중 연령이 높을 수록 노인장기요양보험 이용 상태일 확률이 높아지는 반면 혼인상태에 있는 경우 유의하게 낮았다. 거주지역 경우 도농 복합군 거주자는 준거지역에 비해 노인장기요양보험 이용 상태일 확률이 유의하게 높아지는 것으로 나타났다. 본 연구의 결과 노인장기요양 서비스 이용자가 강한 상태 의존성을 가진다는 사실은 향후 수요예측에 있어 기존 이용자의 이용 기간 증가도 충분히 고려하는 것이 중요함을 시사한다. This study intends to the estimate the determinants and state dependence of long-term care services in Korea. For this purpose, we analyzed the transition patterns among three states of long-term care service utilization over time by using the Korea Welfare Panel Study data with the random effect multinomial logit model. It is found that the result showed a strong state dependence in long-term care service utilization. Especially, long-term care insurance for the elderly showed a strong state dependence among others. Among the individual demographic characteristics, the higher the age, the higher the probability of using long-term care insurance for the elderly, while the lower the probability when married. The characteristics of the residential region showed that the residents of the urban-rural integrated region had a significantly higher probability of using long-term care insurance than the reference region. The results of this study suggest that the long-term care service users have a strong state dependence, which means that it is important to take into account the increase in the utilization period of existing users in future demand forecasting.

[내가 읽은 책] 건축 이전의 건축, 공동성

최장원(Choi, Jang-Won) 대한건축학회 2015 建築 Vol.60 No.1

노년기 경제적 스트레스가 노화불안에 미치는 영향

최장원(Jang-Won Choi) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.1

본 연구는 노년기 경제적 스트레스가 노화불안에 미치는 영향에서 자발적 봉사활동 동기, 그리고 연령의 상호작용효과를 검증하여 노화불안에 대한 개입 방안을 제공 하고자 함에 있다. 이를 위해 노인종합복지관을 이용하는 노인 211명을 대상으로 자기보고식 설문조사를 실시하였고, 불성실한 응답을 제외한 182명의 노인을 대상으로 빈도분석, 기술통계, 다중회귀분석을 실시하였다. 분석결과 노년기 경제적 스트레스는 노화불안을 높였지만, 자발적 봉사활동 참여 동기는 노화불안을 낮추는 것으로 나타났다. 또한 경제적 스트레스가 노화불안을 높이는 가운데 봉사활동에 대한 만족도가 높아 자발적으로 참여하는 노인일수록, 그리고 고연령 봉사자일수록 노화불안이 더욱 낮은 것으로 나타났다. 이를 바탕으로 노인 삶의 질 제고를 위한 정책적, 실천적 방안을 제언하였다. The purpose of this study is to examine the effect of economic stress on aging anxiety focusing on the moderating effect of motivations for participation in volunteering, and age. In order to accomplish these purposes, a total of 182 elderly who have been volunteering were used for final analysis. The results from the multiple regression showed that economic stress increases aging anxiety among elderly and motivations for participation in volunteering are likely to mitigate aging anxiety. Also, aging anxiety is expected to be lower as people are older and have higher self-oriented motivation for volunteering. Based on the result, political and practical implications are discussed.

청소년의 아동학대 피해와 비행의 관계에서 지역사회 범죄 목격 경험에 대한 연구

최장원(Jang Won, Choi),김준범(Joon Beom, Kim) 한국범죄심리학회 2022 한국범죄심리연구 Vol.18 No.1

본 연구는 청소년의 아동학대 및 지역사회 범죄 목격 경험이 비행 유형별 발생 위험에 미치는 영향을 실증적으로 검증하여 비행 예방을 위한 지역사회 기반의 개입의 단초를 제공하는 것을 목적으로 한다. 이를 위해 한국형사·법무정책연구원에서 2014년 진행한 청소년 대상 범죄피해조사 에서 중, 고등학생 총 7,109명을 대상으로 수집한 횡단 데이터 중 본 연구의 주요변수에 대한 결측치를 제외한 최종 6,883명의 데이터를 활용하여 분석을 실시하였다. 주요 분석 결과는 다음과 같다. 첫째, 전체 연구대상자 중 약 8.6%(n=593)는 아동학대 및 지역사회 범죄 목격 경험이 있는 것으로 나타난 가운데, 세부적으로 아동학대만 경험한 집단이 전체의 6.1%(n=419)로 가장 많았고, 그 다음은 지역사회 범죄 목격 집단이 1.9%(n=134), 그리고 앞선 두 경험의 중복경험 집단은 0.6%(n=40)로 나타났다. 둘째, 아동학대 및 지역사회 범죄 목격 경험이 각 경험이 중첩될수록 그 위험이 배가되는 경향을 보였으며, 중복경험 집단의 각 위험비는 폭력비행 14.73배, 재산비행 10.1배, 지위비행 8.5배, 성비행의 경우는 7.25배 높은 것으로 나타났다. 이러한 결과를 바탕으로 청소년 비행 예방을 위한 처벌 중심의 논의를 넘어 사회 공동체적 책임의식 확산과 가정 및 지역사회에서의 예방을 위한 정책적 방안을 논의하였다. This study aims to examine the effect of the co-occurrence of child maltreatment (CM) and witness community crime (WCC) on juvenile delinquency in order to provide communiby-based preventive strategies. Cross-sectional data of a sample of 6,883 adolescents were obtained from the by Korean Institute of Criminology and Justice in 2014. Main results are as follows. First, participants who exposed to CM and/or WCC occupied 8.6% (n=593); only CM was 6.1% (n=419), only WCC was 1.9%(n=134), and co-occurrence was 0.6% (n=40). Second, odds ratios for each subtype of delinquency, except for sexual delinquency, were higher among co-occurrence groups than others; violent delinqucy 14.73 times, property delinquency 10.10 times, status delinquency 8.50 times, and sexual delinquency 7.25 times. Based on results, needs for shared awareness of social responsibility beyond punishment, and community-based preventive strategies against crime suggested.

전산 유체역학을 통한 베개 높이가 폐쇄성 수면 무호흡증에 미치는 영향 분석

최장원(Jang-Won Choi),박세현(Se-Hyun Park),김원태(Won-Tae Kim),유민규(Min-Gyu Yu),이호석(Ho-Seok Lee),허재식(Jae-Sig Huh),김경욱(Kyung-wuk Kim),최영호(Young-Ho Choi),김형호(Hyoung-Ho Kim) 대한기계학회 2020 大韓機械學會論文集B Vol.44 No.8

본 연구의 목적은 전산 유체역학을 사용하여 베개 높이에 따라 상기도에서 폐쇄성 수면 무호흡증의 발생에 대하여 분석하는 것이다. 머리 지지대의 초기 높이가 0cm인 모델 1, +2㎝인 모델 2, 그리고 +4㎝인 모델 3에 대한 CB-CT(cone beam CT) 이미지를 통해 만들어진 3차원 상기도 모델이 전산 유체역학을 위해 이용되었다. 인두에서 6개의 단면은 각 모델에 대하여 최대 흡기, 휴식기, 호기에 따라 속도, 압력, 면적의 변화를 측정하기 위해 사용하였다. 머리 지지대의 높이는 상기도의 유동 특성과 관계가 있었다. 모든 모델에서 단면적이 좁을수록 큰 유량과 음압이 발생하였지만, 모델 2에서 상기도 저항과 연인두에서의 평균 압력이 가장 낮았다. 그러므로 적절한 베개 높이를 선택하는 것은 폐쇄성 수면무호흡증을 줄일 수 있을 것으로 보인다. The objective of this study was to investigate the development of obstructive sleep apnea (OSA) in the upper airway according to pillow height using computational fluid dynamics (CFD). Three-dimensional upper airway models made from cone-beam computed tomography images (using the initial height of the head support for model 1 and 2- and 4-㎝ heights of the head support for models 2 and 3, respectively) were used for the CFD. Six cross-sectional planes in the pharynx were used to measure the variations of the area, pressure, and velocity at maximum inspiration, rest, and maximum expiration at each plane according to the models. The height of the head support was related to the fluid characteristic information in the upper airway. In all the cases, the narrower the cross-sectional area, the greater the flow rate and negative pressure. However, model 2 showed the lowest upper airway resistance and average pressure in the velopharynx. Therefore, selection of the appropriate pillow height might reduce the incidence of OSA.

데이터 주권 분야 및 시스템 연구 실행 의제

최장원(Jang-won Choi),김은진(Eun-jin Kim),이용호(Yong-ho Lee) 한국정보통신학회 2019 한국정보통신학회 종합학술대회 논문집 Vol.23 No.2

세계 주요국은 데이터 경제로 전환하기 위한 전략 수립과 함께 데이터 패권 경쟁에 돌입하였고, 데이터 민주주의 개념이 부상하며 데이터 주권도 대두되고 있다. 본 논문에서는 데이터 경제의 급속한 진전에 대비하고 있는 세계 각국과 우리나라의 현황을 분석하고, 새로운 데이터 시대에 대응하기 위해 개인의 데이터 주권 강화를 위한 시사점과 정책의제를 제시하고자 한다. Major countries in the world have entered into a data hegemony competition with a strategy to transform into a data economy. The concept of data democracy has emerged, and data sovereignty has emerged. In this paper, we analyze the current state of the world and Korea in preparation for the rapid development of the data economy. In order to cope with the new era of data, this paper suggests implications and policy agenda for strengthening individual data sovereignty.

Anti-diabetic Effect of the Exopolysaccharides (EPS) Produced from Cordyceps sinensis on ob/ob Mice

Jang Won Choi(최장원) 한국생물공학회 2011 KSBB Journal Vol.26 No.1

Anti-diabetic effect of the exopolysaccharides (EPS) produced from submerged mycelial culture of Cordyceps sinensis (Cs) was studiedin a type II diabetic animal model (C57BL/6J ob/ob). This study was designed to determine whether Cs-EPS improves clinical symptoms of type II diabetes in ob/ob mice. After Cs-EPS treatment at doses of 200 mg/kg body weight, the fasting blood glucose levels decreased by 47% after 7 weeks compared with those of the control mice. According to the oral glucose tolerance test, the glucose levels recovered its baseline after 120 min in Cs-EPS-treated mice, although the blood glucose levels increased significantly after 30 min. On the other hand, the control group (not-treated) did not recovered its initial level of glucose after 120 min. Furthermore, food intake, body weight, total plasma cholesterol and triglyceride concentrations in ob/ob mice treated with Cs-EPS were significantly decreased, compared with those in control ob/ob mice. Cs-EPS treatment increased significantly the plasma insulin level and the expression of leptin mRNA in adipose tissue of Cs-EPS-treated ob/ob mice. From these results, it is demonstrated that Cs-EPS could be effective for regulating normal blood glucose levels by increasing the amounts of plasma insulin and leptin expression in ob/ob mice, indicating that this compound could be a candidate material as a dietary supplement to control hyperglycemia in patients suffering from type II diabetes.

고초균에서 흰이빨참갯지렁이 페리틴 단백질의 분비 및 사료 효율성

최장원(Jang Won Choi) 한국생물공학회 2016 KSBB Journal Vol.31 No.2

Ferritin is known to regulate iron metabolism and maintain iron in a variety of the eukaryotic organisms. The region encoding the mature ferritin (0.47 kb, H-type) of Periserrula leucophryna was amplified using the designed primers including restriction enzyme site and termination codon and subcloned in frame to the pRBAS secretion vector containing the signal sequence, RBS, and promoter of amylase gene (E. coli-Bacillus shuttle vector), resulting in recombinant pRBASPLF vector. Recombinant ferritin (18 kDa) was correctly processed and secreted from Bacillus subtilis LKS strain harboring the pRBAS-PLF vector and quantitatively analyzed by SDS-PAGE and western blot, respectively. Secretion of the ferritin was optimized by culture conditions (host, medium, temperature, nitrogen source) in 3 L batch culture and 5 L jar fermenter. Finally. the ferritin was largely produced using 50 L fermenter as the following conditions; at 30℃, 150 rpm, 1 vvm in Bacillus subtilis LKS using PY medium. The secreted ferritin was maximally measured (approximately 177.6 ug/ml) when the cell density reached to 14.4 at OD600 (20 h incubation). The iron binding activity was confirmed by Perls" staining in 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in the culture broth after secretion. Biologically, the culture broth and powder type containing ferritin were tested for possibility as feed additive in chicken broiler. As a result, the ferritin stimulated the growth of chick broil and improved feed efficiency and production index.

임시건축의 도시-건축적 의미와 유효성에 관한 연구 - 60, 70년대 아방가르드 건축과 들뢰즈(G. Deleuze)의 철학적 개념을 중심으로 -

최장원(Choi, Jang Won),전영훈(Jeon, Young Hoon) 대한건축학회 2024 대한건축학회논문집 Vol.40 No.3

This study explores the urban-architectural significance and effectiveness of temporary architecture along with its theoretical framework. It begins by examining avant-garde architecture from the 1960s and 1970s and the subsequent changes in era. Deleuzes philosophical concepts were employed to analyze avant-garde architecture and its correlations with temporary architecture. This study highlights the influence of avant-garde architecture on the emergence and expansion of temporary architecture. Furthermore, it reevaluates changes in contemporary temporary architecture since the 1980s and investigates examples with transboundary characteristics. Temporary architecture was classified into three main types: connection-oriented, re-territorialized, and de-centered, each with specific characteristics. Analysis revealed that attributes like rhizomatic, becoming, and virtualite played crucial roles in amplifying the dynamic nature of temporary architecture. Additionally, this study defined contemporary temporary architecture as one that actively interacts with various elements within and around architecture, including the urban environment, users, and programs. This discourse on the validity of temporary architecture offers a fresh perspective and contributes significantly to both research and practice in the field.