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서해안 양식장에서 분리한 Edwadsiella tarda의 특이 bacteriophage 특성
이찬흔 ( Chan Heun Lee ),허용주 ( Yong Ju Heo ),백민석 ( Min Suk Baek ),이정은 ( Jung Uen Lee ),강자영 ( Ja Young Kang ),한미정 ( Mi Jung Han ),경서봉 ( Se Bong Kyoung ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2
Edwardsiella tarda (E. tarda)는 담수 및 해수어에 광범위하게 감염을 일으키는 그람 음성 박테리아이다. 본 연구에서는 서해안 넙치 양식장에서 분리한 E. tarda에 대해 특이 용균을 보이는 파아지를 분리하여 파아지의 활성에 미치는 환경적 요인인 pH와 배양온도의 영향 등을 조사하였다. 또한 어류모델로서는 담수어종인 Nile tilapia (Oreochromis niloticus)를 이용하였으며 E. tarda와 파아지와의 상호작용을 알아보았다. pH 안정성 실험에서는 담수보다 해수에서 높은 활성을 보였으며 온도 안정성 실험 결과 50℃까지 일정한 활성을 보였다. Tilapia 감염 실험에서는 파아지와 E. tarda를 접종하였다. 그 결과 실험군은 대조군에 비해 24시간째까지 E. tarda수가 일정하게 감소하는 것을 확인하였으며 파아지는 처음 접종 양보다 약간 증폭됨을 알 수 있었다. 본 연구의 결과는 E. tarda에 의해 발생되는 질병을 특이 파아지를 이용하여 제어할 수 있음을 암시해준다. Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E. tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investgated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to 50℃. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.
Vibrio alginolyticus에 대한 특이 bacteriophage의 생물학적 특성
허용주 ( Yong Ju Heo ),이찬흔 ( Chan Heun Lee ),백민석 ( Min Suk Baek ),안현미 ( Hyun Mi Ahn ),황요셉 ( Yo Sep Hwang ),박권삼 ( Kwon Sam Park ),최상훈 ( Sang Hoon Choi ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6
Vibrio alginolyticus, a marine f ish and shellf ish pathogen, has been found at a high frequency around the coastal areas of Korea. Vibrio alginolyticus was purif ied from various diseased f ish, and a V. alginolyticus-specif ic bacteriophage was isolated from seawater obtained from f ish farms located on the west coast of Korea. In a bacterial lysis experiment using the phage and antibiotics, tetracycline, 103 cfu/ml of V. alginolyticus were completely lysed by both the phage and the antibiotic, suggesting that the purif ied phage in the study could be utilized as an alternative to antibiotics in the control of f ish and shellf ish diseases caused by V. alginolyticus.
서해안 양식장에서 분리한 Vibrio alginolyticus의 특이 bacteriophage에 대한 구조적 특성
허용주 ( Yong Ju Heo ),이찬흔 ( Chan Heun Lee ),백민석 ( Min Suk Baek ),안현미 ( Hyun Mi Ahn ),황요셉 ( Yo Sep Hwang ),박관하 ( Kwan Ha Park ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Vibrio alginolyticus (V. alginolyticus), which is one of bacterial pathogens evoking severe infection in fish and shellfish as well as in human has been found at high frequency around all coast areas in Korea. Both V. alginolyticus and V. alginolyticus specific bacteriophage were isolated from sea water and various fishes from fish farms on west coast in Korea. In a morphological study based on electron microscope, the purified phage appeared to be composed of hexagon head of 60 nm and short tail of 20 nm. In the denatured SDS-PAGE analysis, the structural proteins of the phage were found to be 7 different protein fractions ranging from 37.8 to 198 kda. The kind of nucleic acid of the phage was ascertained to a double stranded DNA.
유산균 생산 후 폐기되는 부산물 첨가 사료의 급이가 잉어(Cyprinus carpio)의 성장률, 선천성 면역, 면역연관 유전자 발현 및 항균효과에 미치는 영향
최재혁,정상목,양은총,장태원,이찬흔,박관하,최상훈,Choi, Jae Hyeok,Jung, Sang Mok,Yang, Eun Chong,Jang, Tae Won,Lee, Chan Heun,Park, Kwan Ha,Choi, Sanghoon 한국어병학회 2022 한국어병학회지 Vol.35 No.1
This study has been performed to investigate the potential effects of by-product discarded after probiotics production (BPPP) on growth performance, immune gene expression, innate-immunity status, and disease resistance of carp, Cyprinus carpio. For 3 weeks, carp were fed four diets containing different levels of BPPP at 0, 0.1, 0.2 and 0.5% per kg of normal diet. Every 7 days of feeding, immune-related gene expression, serum lysozyme activity and ACH<sub>50</sub> were analyzed. Growth rates and challenge test with E. tarda were conducted after 3 weeks of BPPP feeding. Both lysozyme activity and ACH<sub>50</sub> were significantly (p<0.05) increased in all BPPP supplemented groups compared to the control at every 7 day for 3 weeks of feeding trial. The gene expression of pro-inflammatory cytokines, IL-1β and TNF-α was significantly (p<0.05) up-regulated until 21 days of feeding in all groups except for 0.2% group on day 7 post feeding. The anti-inflammatory cytokine IL-10 gene expression was only significantly (p<0.05) increased in 0.1% group on day 7 and decreased (p<0.05) on day 14 in all BPPP supplemented groups. On day 21, the IL-10 gene expression was augmented (p<0.05) in all groups. SOD gene expression was significantly (p<0.05) increased compared to the control on day 14 and 21 post feeding, whereas no significant difference was observed on day 7. In challenging test, 0.2%, 0.1%, 0.5% and control group showed 80%, 70%, 60% and 40% of survival rate, respectively. Feed conversion rate was only improved in 0.5% group. In conclusion, the present study indicates that dietary BPPP suplementation improved growth performance, innate immune response and bactericidal activity in carp.
진피(Citrus unshiu Peel)추출물이 첨가된 사료의 급이가 넙치의 성장률 및 항스쿠티카충 효과에 미치는 영향
방석진 ( Seok Jin Bang ),최재혁 ( Jae Hyeok Choi ),정상목 ( Sang Mok Jung ),강인성 ( In Sung Kang ),이찬흔 ( Chan Heun Lee ),박관하 ( Kwan Ha Park ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2020 한국어병학회지 Vol.33 No.2
In an attempt to find a feed additive showing an anti-scuticociliate effect, extracts from Citrus unshiu Peel were tested against virulent scuticociliate infection. The most effective anti-scuticociliate killing activity in vitro was observed in the extract squeezed from homogenizing water-soaked dried tangerine peel (DTP). In addition, we have investigated the effect of DTP as a feed additive on growth rate and anti-parasitic activity of olivaceus flounder. DTP extract added diets (0.1, 0.5, 1, and 5%/feed weight) were fed to flounder for 7 days for checking a growth rate and 14 days for a challenging test. As a result, the feed conversion rate was significantly improved only in 1% DPT extract group compared to the control and 0.5% DTP extract fed group showed 100% of survival rate in the challenge test, all of which indicating that DTP extract would be a potential feed additive against scuticociliatosis.