인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

P.intermedia의 유전자 이종성과 가족내 전이에 관한 연구

이승민,김각균,정종평,Lee, Seoung-Min,Kim, Kack-Kyun,Chung, Chong-Pyoung 대한치주과학회 1995 Journal of Periodontal & Implant Science Vol.25 No.1

P. intermedia are considered an important pathogen in adult periodontitis, rapidly progressing periodontitis, refractory periodontitis, pregnancy gingivitis, acute necrotizing ulcerative gingivitis, pubertal gingivitis. So far 2 DNA homology groups and 3 serotypes of P. intermedia have been reported but there is no data available as yet regarding genetic diversity for the species P. intermedia. The purpose of this study is to investigate, using bacterial DNA restriction endonuclease analysis, genetic diversity between individual strains of P. intermedia which are indistinguishable by serotyping and biotyping, occurrence of an intrafamilial transmission and genetic heterogeneity between P. intermedia strains isolated within a patient and within the same serotypes. The families who have had no systemic disease, no experience of periodontal treatment for the previous 1 year and no experience of antibiotics for the previous 6 months were selected and subgingival plaque was collected at 4 sites in each person and incubated in the anaerobic chamber. P. intermedia were identified by colony shape, gram stain, biochemical test, SK-I03(Sunstar Inc.) test and IIF using monoclonal antibody was perfomed for the determination of serotypes. P. intermedia strains were grown in BHI broth and whole genomic DNA was extracted and digested by restriction endonuclease. The resulting DNA fragments were separated by agarose gel electrophoresis, stained and photographed under UV. As the results of this study, intrafamilial vertial transmissions could be assessed in 2 families and horizintal transmissions in another 2 families. There were different DNA digest patterns within a patient, so P. intermedia showed that individuals could be colonized by multiple clonal types at anyone time. And different serotypes could be found within a patient and in the same serotype within a patient, obvius genetic heterogeneity could not be assessed. But in the same serotype in different famies, there were differences in the DNA digest patterns.

인간 포배기 배아의 초자화 동결에 관한 연구: II. 초자화 동결이 포배기 배아의 착상 및 임신에 미치는 영향

김수희,이상원,이주희,강상민,오희정,이승민,이성구,윤혜균,윤산현,박세필,송해범,임진호,Kim, Su-Hee,Lee, Sang-Won,Lee, Ju-Hee,Kang, Sang-Min,Oh, Hee-Jeong,Lee, Seoung-Min,Lee, Seong-Goo,Yoon, Hye-Gyun,Yoon, San-Hyun,Park, Se-Pill,Song, Hai- 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: This study was conducted to investigate the effect of vitrification on the implantation and the pregnancy of human blastocysts. Method: The transfer of the frozen-thawed blastocysts by the slow freezing or vitrification was performed between January 1998 and July 1999. The zygotes derives from IVF were cocultured with cumulus cells in YS medium containing 20% hFF for 5days. Two or three of the best balstocysts produced on day 5 were transferred into the uterus, and then supernumerary blastocysts were randomly divided into two groups. One was frozen by slow freezing and the other was frozen by vitrification method. The slow freezing procedure was performed in two steps (5% glycerol and 9% glycerol + 0.2 M sucrose for 10 min, respectively) using programmed freezer ($-2^{\circ}C$/min to $-7^{\circ}C$, manual seeding at $-7^{\circ}C$, $-0.3^{\circ}C$/min to $-38^{\circ}C$ and plunged into $LN_{2}$). The blastocysts frozen by slow freezing were thawed at $36^{\circ}C$ then removed glycerol in 7 steps. The vitrification procedure was performed in three steps (10% glycerol for 5 min, 10% glycerol + 20% ethylene glycol for 5 min, 25% glycerol + 25% ethylene glycol and directly $LN_{2}$ within 1 min). The blastocysts frozen by vitrification were thawed at $20^{\circ}C$ water then removed cryoprotectant in 3 steps. In each group, thawed blastocysts were cocultured with cumulus cells in YS medium containing 20% hFF for 18h and transferred into the uterus. The implantation rate was evaluated per transferred blastocysts and the pregnancy rate was evaluated per transfers. Results: The survival rate of vitrified group (74.5%) was higher than slow freezing group (68.0%), but not significant. When 98 thawed blastocysts of vitrification were transferred in 40 cycles, 19 pregnancies (clinical pregnancy rate; 47.5%) were established. One miscarriage occurred in the eighth week of pregnancy (ongoing pregnancy rate; 45.0%). 7 pregnancies were ongoing, 11 pregnancies went to term, and 16 healthy infants were born. The Implantation rate was 31.6%. These results were higher than those obtained by the slow freezing (clinical pregnancy rate; 40.3%, ongoing pregnancy rate; 32.5% and implantation rate; 25.3%), but not significant. Conclusion: Vitrification is a simple, quick and economical method when compared to slow freezing. It will be chosen as a good method of human embryo freezing in IVF-ET programs.

한국 전통주택의 실내조경 요소에 관한 연구

이승민(Seoung-Min Lee),심우경(Woo-Kyung Sim) 한국실내조경협회 2000 室內造景 = Journal of the Korean institute of interior Vol.2 No.1

This study was performed to find out kinds of landscape architectural element for knowing how to use them in interior of a traditional house and what is the unique elements for representing Korean mood to compare interior of China house.<br/> Traditional Korean house used much furniture, folding screens, curtains, blinds, paintings, wooden beds, ceramics, lighting fixtures and plants in interior of a traditional house. Korean furniture was made low and simple and mostly, because of low ceiling, simple interior and Ondol, the Korean under-floor heating system. A Korean-style home was build to go with nature in structure. And its interior surroundings was very dark because its windows were made small for these reasons, the introduction of plant were rare.<br/> The Interior space of China was made up of sitting and standing space, and naturally two kinds of Interior furniture were used. And lastly, in the other elements, with a few exceptions of highly decorative and gorgeous things, there had been very active introductions of flowerpots and bottles.

영상의학의 오류와 불일치는 불가피한 것인가 혹은 피할 수 있는 것인가? 중추신경계 증상으로 촬영한 뇌 자기공명영상에서 간과된 청신경 종양 4예

이승민(Seoung-Min Lee),전정배(Jeong-Bae Jeon),이동희(Dong-Hee Lee) 대한두개저학회 2020 대한두개저학회지 Vol.15 No.1

Diagnostic error can be defined as “diagnosis that was unintentionally delayed (sufficient information was available earlier), wrong (wrong diagnosis made before the correct one), or missed (no diagnosis ever made), as judged from the eventual appreciation of more definitive information”. According to previous studies, estimated rate of errors and discrepancies in radiology are 3%-5% of studies reported. In 2014, Kim and Mansfield published classification system for radiological error. Errors and discrepancies in radiologic finding are somewhat inevitable and evitable. However, its impact to patients may be significant on some disease entities. Diagnostic error can give health system mistrust and dissatisfaction from both patients and doctors. We report four cases of acoustic neuroma that was not diagnosed on brain magnetic resonance images by diagnostic radiologists but detected by otolaryngologist later. Authors suggest that it is better to improve patient safety and reduce diagnostic error for clinicians to review carefully the images that was taken for other reasons but included area of interest. Clinicians should review the images by themselves as well as read radiologist’s interpretation.

체외수정시술시 임신율 향상을 위한 새로운 요법 ( 변형장기요법 : MLL ) 에 관한 연구

이성구(Seong Goo Lee),이승민(Seoung Min Lee),이용찬(Yong Chan Lee),정재훈(Jae Hoon Jung),이원돈(Won Don Lee),임진호(Jin Ho Lim),장윤석(Yoon Suk Chang) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.8

목적 : 체외수정시술에 있어서 임신을 향상을 위한 새로운 요법 (변형장기요법)의 효용성을 평가하였다. 연구 방법 : 2001년 3월부터 7개월 동안 반복적으로 2회 이상 보조생식술을 실패한 군 ( Ⅰ군: n=106)에 변형장기요법을 적용하였다. 같은 시기에 장기요법을 시행했던 군 (Ⅱ군: n=315)과 역으로 7개월을 추적하여 I군과 같은 적응이었으나 장기요법을 시행했던 군 (Ⅲ관: n=144)과의 임신율과 착상율을 비교하였다. 결과 : Ⅱ군의 평균 연령은 33.22±4.14로 I군의 35.16±4.35에 비해 유의하게 낮았으며 (p<0.05), Ⅱ군의 embryo quality score는 I군에 비해 유의하게 높았다. 그 외 I관과 Ⅱ군 그리고 Ⅲ군 사이의 IVF-ET 결과, 즉 회수된 난자수, 이식 배아수, 2 PN수, ICSI의 비, 포배기 이식의 비, 수정율, 8 ㎜ 이상되는 자궁내막을 가진 환자의 비 둥은 유의차가 없었다. Ⅱ군에서의 착상율 (25.2 vs. 17.1%, p=0.02)은 특히 31-35세 사이에서 1군에 비해 유의하게 높았다. Ⅱ군의 난관인자 불임환자에서 35세 이하의 착상율과 31-35세 군의 임신율은 I군에 비해 유의하게 높았다. Ⅰ군과 Ⅲ군의 비교에서 Ⅰ군의 36-40세 군에서 인심율 (47.2 vs. 12.8%, p=0.001)과 착상율 (15.2 vs. 5.5%, p=0.009)이 Ⅲ군에 비해 유의하게 높았다. Ⅰ군의 자궁내막증 환자의 임신율과 착상율 (특히 36-40세 군)이 Ⅲ군보다 유의하게 높았고 35세 미만의 원인불명의 불임환자에서의 착상율도 I군이 Ⅲ군에 비해 유의하게 높았다. 결론 : 새로운 난포자극 방법인 변형장기요법은 자궁내막증 환자와 원인불명의 환자에서 착상율과 임신 율을 높이는 효과가 있다고 사료되며, 분자생물학적인 연구를 포함한 폭넓은 연구를 시행하여 자궁내막의 수용능을 높이는 요인들을 규명해야 할 필요성이 있다고 생각된다. Objective : To evaluate the efficacy of new protocol (Modified luteal long; MLL protocol) to improve the clinical outcomes in IVF-ET. Methods : New protocol was applied to repeated ART failure group for 7 months (Group I , n=106) after informed consent. Outcomes of new protocol group were evaluated and compared to conventional long protocol group (Group Ⅱ, n=315) prospectively and compared to same indication group with Group I (Group Ⅲ, n=144) historical prospectively. Results : There were no differences in the clinical characteristics except patient`s age between Group I and II (35.16±4.35 vs. 33.22±4.14, p<0.05). Outcomes of IVF-ET, such as numbers of retrieved oocytes, numbers of transferred embryos, numbers of 2 PN, rates of ICSI, rates of blastocyst ET, rates of fertilization, ampules of gonadotropin between Group I & Ⅱ , and Group I & Ⅲ, respectively. But the embryo quality score of GroupⅡ was significantly higher than Group I . There was significant increase of implantation rate (25.2 vs. 17.1%, p=0.02) in Group II compared to Group I , especially in 31-35 years old group. Implantation rate of less than 35 years old group and clinical pregnancy rate of 31-35 years old group in Group Ⅱ tubal factor patients were significantly higher than Group I tuba] factor patients. Clinical pregnancy rate (47.2 vs. 12.8%, p=0.001) and implantation rate (15.2 vs. 5.5%, p=0,009) of Group I was significantly higher than Group Ⅲ, especially in 36-40 years old group. Implantation rates of ES factor patients (especially in 36-40 years old) and unknown factor patients (especially less than 35 years old), and clinical pregnancy rate of ES factor patients (especially in 36-40 years old) were significantly higher in Group I than Ⅲ. Conclusion : These results suggest that new protocol may play some role in the increase of endometrial receptivity. Further investigation, including molecular research work will be needed to clarify the factors concerned in the increase of implantation rate.

비닐하우스 재배지역의 비점오염원의 유출특성

최서현 ( Seo Hyun Choi ),이승민 ( Seoung Min Lee ),권구호 ( Ku Ho Kwon ),김시원 ( Si Won Kim ),민경석 ( Kyun Suk Min ) 한국물환경학회 ( 구 한국수질보전학회 ) 2010 공동 추계학술발표회 Vol.2010 No.-

Water stress가 오이의 종자 발아와 초기 생육에 미치는 영향

윤경은(Yoon Kyung-Eun),이인돈(Lee In-Don),김미심(Kim Mi-Shim),이승민(Lee Seoung-Min) 한국원예학회 1988 한국원예학회 학술발표요지 Vol.6 No.1