인공수정용 돼지 액상정액 세균오염도 조사 및 정액유래 주요 바이러스성 질병 감염률 조사

손병국 ( Byeong Guk Son ),박호정 ( Ho Jung Park ),김은경 ( Eun Gyeong Kim ),이종민 ( Jong Min Lee ),황보원 ( Bo Won Hwang ),허정호 ( Jung Ho Heo ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.4

Bacteroiospermia is a frequently finding in fresh raw and extended porcine semen and can results in detrimental effects on semen quality and longevity. This study aims to evaluate the type of bacterial contaminants in raw and extended porcine semen and the reducing effect of antibiotic test. To investigate bacterial contaminants, out of 387 sample (raw semen 201, extended semen 186) were collected from 6 artifical insemination centers in Gyeongsangnam-do, were inoculated onto blood agar and MacKonkey agar, respectively. Bacterial colonies were selected after culturing for 48 hours, at 37℃, followed by Gram staining, KOH test, oxidase test, catalase test and eventually identified using VITEK System. Total 15 genus and 24 species of bacteria were isolated from these semen samlpes. In raw semen, the most prevalent contaminants were Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Staphylococcus auricularis, Delftia acidovorans, Acinetobacter lowffii, S. aureus and others. And in extended porcine semen, A. lowffii, S. aureus, S. auricularis and other bacteria were identified. Most of them was G (-), which is nonpathogenic bacteria. It seems that bacterial contaminants in fresh raw and extended porcine semen originated from multiple sources at the farms/stud, and were from animal origin and non-animal origins. Whereas, the 7 virus which is known to be detected in porcine semen in 75 cases was not detected. This results showed that removal of bacterial contamination in raw and extended porcine semen is essential and farms were kept for biosecurity and individual hygienes.

경남 중부지역 도축장 출하우의 요네병 감염실태 조사

손병국 ( Byeong Guk Son ),석주명 ( Ju Myoung Seok ),장은희 ( Eun Hee Jang ),지대해 ( Dae Hae Ji ),신정섭 ( Jeong Seop Shin ),황보원 ( Bo Won Hwang ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.1

Mycobacterium avium subsp. paratuberculosis is the pathogen of paratuberculosis called Johne`s disease. Johne`s disease is hardly eliminated because of its long latent period and continuous dissemination, so it is found in ruminants worldwide and can cause substantial economic losses in cattle. It has been reported in many studies on the distribution of Johne`s disease in some provinces of Korea that not many, but noticeable numbers of infected cows have been detected since the first detection in 1984. The aims of this study was to investigate the prevalence of Johne`s disease obtained from slaughtered cattle in central area of Gyeongnam province, Korea. In this study, the ELISA serum antibody test and PCR were employed on a total of 240 blood and ileac substrate samples from slaughtered cattle in two slaughtering and wholesale centers in Gyeongsangnam-do Livestock Veterinary Research Institute Central Branch. Out of the entire 240 blood samples, three (1.3%) were positive by ELISA, while five (2.1%) were suspected cattle. But ileac substrate samples, eight (3.3%) were positive by PCR. By breeds, positive rates of ELISA and PCR in Korean native cattle were 1.3% and 3.5%, respectively, but no positive cows were found in dairy cattle. By provinces, sero-positive rates of Gyeongnam and Gyeongbuk were 1.6% and 1.3%, respectively. And PCR positive rates of Gyeongnam, Gyeongbuk and other provinces were 2.4%, 5.0% and 2.8%, respectively. These results indicate that it requires the nationwide monitoring test and measure to deal with subclinically infected slaughtering cows.

경남지역의 돼지톡소플라즈마병 감염실태 조사

김은경 ( Eun Gyeong Kim ),박호정 ( Ho Jung Park ),손병국 ( Byeong Guk Son ),정명호 ( Myeong Ho Jung ),허정호 ( Jung Ho Heo ),황보원 ( Bo Won Hwang ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.4

Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma. The definitive host of T. gondii is the cat, but the parasite can be carried by the vast majority of warm-blooded animals, including humans. It is often found in the tissues of food animals including pigs and sheep. To determine the regional prevalence of infection with T. gondii, bloods (n=300) from domestic pigs and tissues (n=200) from slaughter pigs in Gyeongnam province were tested using an enzyme-linked immunosorbent assay (ELISA) and a polymerase chain reaction (PCR) for detection of antibody and antigen. A total of 115 sero-positive pigs were identified for a prevalence rate of 38.3%. Of the 50 herds from domestic pigs tested, 34 had at least one sero-positive pig for a herd prevalence rate of 68.0%. Sero-positive rates of pigs in fattening farm were higher than that of pigs in breeding company. Sero-positive rates of sows were higher than that of growing pigs. Seasonally, sero-positive rates of pigs were highest in winter (80.0%) and lowest in spring (23.8%). According to farm size, sero-positive rates of pigs were higher in small size farms (≤2,000) than that of big size farms (>2,000). However, none of the bloods (n=300) from domestic pigs and tissues (n=200) from slaughter pigs were positive for T. gondii specific DNA by PCR.

Real-time PCR을 이용한 돼지써코바이러스 감염증 진단법 연구

김은경 ( Eun Gyeong Kim ),황보원 ( Bo Won Hwang ),이종민 ( Jong Min Lee ),손병국 ( Byeong Guk Son ),박호정 ( Ho Jung Park ),김도경 ( Tho Kyoung Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4

Assay for the detection and quantification of porcine circovirus type 2 (PCV 2) with the Real-time PCR were developed. TaqMan probe real-time using a set of primer/probe was developed for detection of PCV 2. In this study we applied Real-time PCR assay to 320 samples, collected from pig farms. In 151 of 320 samples, PCV 2 DNA was detected by conventional PCR assay. All samples positive for PCV 2 DNA in conventional PCR assay were also positive in Real-time PCR assay, but 69 of 169 samples that tested negative for PCV 2 DNA in conventional assay were tested positive in TaqMan probe real-time PCR assay. The test of TaqMan probe real-time PCR resulted in detection and quantification limits of 101 copies per sample. TaqMan probe real-time PCR assay increased the number of samples in which PCV 2 was detected by 21%. TaqMan probe real-time PCR assay is very efficient method in contrast to the conventinal PCR, becoming increasingly important method for gene analysis.

PCR을 이용한 축산물 가공식품 내 소고기 성분 검출법 개발

권영철 ( Young Chul Kwon ),하도윤 ( Do-yun Hah ),허윤위 ( Yunwi Heo ),김태규 ( Tae-kyu Kim ),최유정 ( Yoo-jeong Choi ),조대훈 ( Dae-hoon Jo ),남상윤 ( Sang-yun Nam ),손병국 ( Byeong-guk Son ),황보원 ( Bo-won Hwang ),양병선 ( Byoun 대한임상검사과학회 2017 대한임상검사과학회지(KJCLS) Vol.49 No.2

중합효소연쇄반응법을 이용한 축산물 가공식품 내에 존재하는 소고기 성분을 특이적으로 검출할 수 있는 방법을 개발하기 위하여 축산물 가공식품 78종류를 무작위로 선별하였다. 가공식품으로부터 추출한 genomic DNA를 이용하여 소의 미토콘드리아 16S rRNA 염기서열을 이용하여 strain-specific primer를 직접 제작하여 중합효소연쇄반응을 수행한 후, 증폭된 반응산물의 염기서열을 분석 하였다. 축산물 가공식품 내 소고기 성분 검출을 위한 중합효소연쇄반응 수행 결과, 소고기 성분이 함유되어 있는 17개의 축산물 가공식품이 정확히 증폭되었고, 증폭산물의 DNA 염기서열 분석 결과 소의 미토콘드리아 16S rRNA 서열과 95% 이상의 상동성을 보였다. 본 실험에서 제시된 방법으로 축산물 가공식품 내 소고기 성분검출을 적용하였을 시, 소고기 성분이 함유된 축산물 가공식품을 정확하게 감별할 수 있었으며, 나아가 식품 원재료의 허위기재 등에 의한 불량식품 유통 근절 및 종교적 이유로 인한 금기 식품감별 등과 같은 과학적 식품 감시에 기여할 수 있다고 사료된다. Polymerase chain reaction (PCR) was used to detect cattle material from processed meat products. Seventy-eight different commercial processed meat products were purchased from several big food marts. Among them, 17 products contained cattle material (10 samples contained only cattle, 5 samples mixed with cattle and porcine, 2 samples mixed with cattle, porcine and chicken). The genomic DNA was extracted directly from the processed meat products, and strain-specific primer targeting the 16S ribosomal RNA mitochondrial gene was used. All PCR products were cloned into the pGEM-T easy vector and sequenced. Consequently, the PCR products were amplified from 10 processed meat products, which contained only cattle material in our conditions. Furthermore, PCR reactions showed the same results at mixed samples. The DNA sequence obtained from pGEM-T easy/PCR products showed more than 95% identity with Bos taurus 16S rRNA gene using homology analysis. In conclusion, we suggest that the method using PCR, as performed in this study, could be useful in detecting cattle material in processed meat products. Moreover, our system could be applicable in inspection procedures to improve the verification of correct labeling for import and export processed meat products.