http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Epidermal Growth Factor가 난소 기형암종 배아세포주의 생존율에 미치는 효과
김충희,김종수,김나리,김의용,한진,Kim, Chung-hui,Kim, Jong-shu,Cuong, Dang Van,Kim, Na-ri,Kim, Eui-yong,Han, Jin 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2
Human ovarian cancerous cells survive in a way that they trigger the nucleotide excision repair (NER) or double-strand DNA repair (dsDNA) repair mechanism to show resistance to anticancer drugs and activate many kinds of repair protein, thus removing damaged DNAs. Two experiments on the PA-1 human ovarian teratocareinoma cell line that hardly has any expression of epidermal growth factor receptor (EGFR) were conducted in the study; first, EGF-R was transfected and its receptor was obtained. The receptor was investigated in terms of its mutual relations with many kinds of protein concerning NER or dsDNA repair. Second, it was examined what kind impact cisplatin and adriamycin had on the effects of EGF-R over the PA-1 cell line lacking EGF-R. When being administered with cisplatin and adriamycin, Hey and Hey C2 cell lines showed a high level of resistance while PA-1 cell line a high level of sensitivity. Hey and Hey C2 cell lines that are resistant against anticancer drugs exhibited a high level of EGF-R expression while PA-1 cell line that is sensitive to them did a much lower level of the expression. When PA-1 cell line was transfected for the expression of DNA adduct and EGF-R, it showed a higher level of resistance compared to the control group. There was no difference in the expression of DNA repair proteins (DNA- dependent protein kinase, Ku70, and Ku80) between Hey and the PA-1 cell lines. The results indicate that the Hey cell line that is resistant against cisplatin and adriamycin works along the signaling system responding to the changes of EGF-R while the PA-1 cell line that is sensitive to both of them does to the lack of EGF-R.
시료고체상분산처리와 액체크로마토그라피를 이용한 소의 근육, 간 및 천엽에서의 벤지미다졸계 구충제 잔류분석
김충희,김곤섭,박정희,하대식,류재두,손성기,허정호,정명호,김종수,Kim, Chung-Hui,Kim, Gon-Sup,Park, Jung-Hee,Hah, Dae-Sik,Ryu, Jae-Doo,Son, Sung-Gi,Heo, Jung-Ho,Jung, Myung-Ho,Kim, Jong-Shu 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk $C_{18}$($40{\mu}m$, 18% load, endcapped, 2g). A column made from the resultant $C_{18}$/animal tissue matrix was first washed with hexane($8m{\ell}$), following which the benzimidazoles were eluted with acetonitrile($8m{\ell}$). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear($0.991{\pm}0.007$ to $0.996{\pm}0.005$) with average relative percentage recoveries from $62.1{\pm}3.8(%)$ to $92.3{\pm}7.5(%)$ for the concentration range($0.2{\sim}6.4{\mu}g/g$), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2% and 83.5% at condition II, respectively. Condition II showed higher recoveries rates than condition I. These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable for the determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.
Xylazine의 진정효과와 α-adrenergic 수용체 봉쇄약물의 길항효과
김충희,하대식,김양미,김종수,Kim, Chung-hui,Hah, Dae-sik,Kim, Yang-mi,Kim, Jong-shu 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1
The central nervous system depressant effect of xylazine and xylazine-ketamine was studied in chicken and mice. Intraperitoneal injection of xylazine(1~30 mg/kg) and xylazine(1~30 mg/kg)-ketamine(100 mg/kg) induced a loss of the righting reflex in chicken and mice, respectively. These effects of xylazine were dose-dependent. The results obtained were as follows; 1. The effect of xylazine-induced depression was antagonized by adrenergic antagonists having ${\alpha}_2$-blocking activity(yohimbine, tolazoline, piperoxan and phentolamine). 2. Yohimbine was most effective in the reduction of the CNS depression by xylazine. 3. Phenoxybenzamine and prazosin did not reduced CNS depression by xylazine in both species. 4. Labetalol (${\alpha}_1$, ${\beta}_1$-adrenergic antagonist) and propranolol(${\beta}$-adrenergic blocking agent) were not effective in reducing xylazine induced depression. 5. Cholinergic blocking agents (atropine and mecamylamine), a dopaminergic antagonist (Haloperidol), a histamine $H_1$-antagonist(chlorpheniramine), a histamine $H_2$-antagonist(cimetidine), a serotonergic-histamine $H_1$ antagonist(cyproheptadine) were not effective in reducing xylazine-induced depression. 6. Xylazine-induced depression is mediated by ${\alpha}_2$-adrenergic receptors and appears not to be involved in cholinergic, dopaminergic, serotonergic or histaminergic pathways.
승용 디젤엔진의 연소실 압력 변화에 따른 피스톤 온도 특성
김충희 ( Chung Hui Kim ),이기수 ( Gee Soo Lee ),김현철 ( Hyun Chul Kim ),한상욱 ( Sang Wook Han ),유연설 ( Youn Sul Yoo ),김희수 ( Hee Su Kim ) 한국액체미립화학회 2014 한국액체미립화학회 학술강연회 논문집 Vol.2014 No.-
In this study, the characteristics of piston temperature according to the combustion pressure were analyzed in a passenger car diesel engine. The peak combustion pressure was increased from 170bar to 210bar by adjusting the control parameters such as injection timing and intake pressure. In addition, effects of the shapes and locations of cooling gallery inside the re-melting piston on the piston temperature were evaluated. As the combustion pressure increased, the average piston temperature increased up to 21% in the base piston, while that in the piston with the re-melting technique and larger cooling gallery area increased up to 18 %. On the other hand, the piston with the larger cooling gallery area as well as raised cooling gallery to the piston crown side showed a similar temperature level with the base piston at the 170bar combustion pressure condition.
동물보건사의 평가를 위한 척도개발의 필요성에 대한 제언
김충희(Chung-Hui Kim) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.1
South Korea's animal nursing certificate is a national certificate. The first examination was held in 2022 and 2,544 animal nurses were produced. They work at veterinary hospitals to perform various tasks while assisting veterinarians in treatment of animals. In this process, they sometimes suffer from stress accompanied by physical and mental pain due to friction with customers, conflicts with colleagues, failure to handle work due to inexperience in medical assistance, physical damage caused by companion animals, and fatigue due to overwork. However, since their job has been just made for the first time, there is no tool to assess their condition. Although there are nurses who assist doctors as a similar occupation, since they do not deal with animals, borrowing the assessment tool for them may lead to errors. Therefore, the development of professional assessment tools for animal nursing is acutely necessary. When the tools necessary for assessment of animal nursing were reviewed, it could be seen that tools for assessment of animal nurses’ “job stress, job satisfaction, job fatigue, life satisfaction, self-efficacy, turnover intention, job performance, job embeddedness, work performance, and self-esteem” are necessary. It is hoped that these assessment tools will be developed as soon as possible to improve animal nurses’ pride in their job with enhancement of their work ability, improvement of the management of animal hospitals, qualitative enhancement of patient treatment, and increases in work efficiency and efficiency based on the results of assessment of animal nursing and many studies on animal nursing.
T-2 toxin을 투여한 닭에서 Comet assay 방법을 이용한 DNA 손상 평가와 독성
하대식(Dae Sik Hah),허정호(Jung Ho Heo),이국천(Kuk Cheon Lee),조명희(Myung Heui Cho),김국헌(Kuk Hun Kim),김충희(Chung Hui Kim),류재두(Jae Du Lue),이승환(Seung Hwan Lee),김곤섭(Gon Sup Kim),김의경(Eui Gyung Kim),김종수(Jong Shu Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.2
This study was designed to evaluate the possible DNA damaging effects of T-2 toxin using an alkaline single cell gel electrophoresis (SCGE) comet assay and also to investigate toxic effects in chickens. A total of 20 chickens were used in these experiments. Graded concentrations of dietary T-2 toxin (0, 4, 8, and 16 ㎍/g of diet) were given to groups of 5 broiler chickens. In comet assay, The DNA damage was analysed by the tail extent moment (TEM) and tail length (TL), which were used as markers of DNA strand breaks in SCGE. A significant dose-dependent increase in the extent of DNA migration as well as in the percentage of cells with tails was observed after treatment with T-2 toxin (P<0.05). Treatment with the low T-2 toxin (4 μ/g of diet) induced a relatively low level of DNA damage in comparison with the high T-2 toxin (16 μ/g of diet) group. The growth rate was significantly reduced by concentrations of 8, and 16 μ/g of diet (P < 0.05). The feed conversion ratio were significantly affected by any concentrations (P < 0.05). The relative weight of the spleen, and lung was decreased by the growth inhibitory concentrations. The bursa of Fabricius, thymus, and kidney were decreased in relative weight by concentrations of 16 μ/g of diet. The relative weight of the liver and heart were unaffected. The hemoglobin (Hb), hematocrit (HCT), and mean corpuscular hemoglobin (MCH) were decreased at concentration of 16 μ/g of diet. As compared with control chickens, there was no marked change in serum components except uric acid in T-2 treated chickens. All lymphoid tissues retained atrophic and lymphoid cell depletion throughout the three weeks trial.
하대식,김충희,김곤섭,김의경,김종수,Hah, Dae-sik,Kim, Chung-hui,Kim, Gon-sup,Kim, Eui-gyung,Kim, Jong-shu 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.3
To assess the antioxidative activity of 12 medicinal plants on lipid peroxidation, twelves traditional medicinal plants extracted with 95% methanol were investigated the antioxidative activity using DPPH, thiocyanate acid method, and thiobarbituric acid (TBA) methods. Out of 12 medicinal plants extracted with methanol, the extraction yields of Sedum kamtschaticum was the highest values (49.46%) among them and Geranicum sibiricum, Saururus chinensis root (R), Agrimonia pilosa leaf (L), Agrimonia pilosa root was the lowest value (9.97%). Radical scavenging effect of the selected traditional medicinal plants extracted from different extract solution were examined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical method. Antioxidative activity of methanolic extracts was higher than those of ethanol and n-hexane extracts. Scavenging effects in Sedum kamtaschaticum (R) determined by DPPH radical showed the highest among the 12 plants. The antioxidative effects of the first four medicinal plants were similar to those of butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT), but higher than that of tocopherol, which was used as a handled control. Antioxidative effects of each indicated concentration of the methanolic extracts on linoleic acid by thiocyanate method was the highest in Sedum kamtschaticum and followed by Geum japonicum and Agrimonia pilosa and their antioxidative effect were similar to those of BHA, and BHT, but higher than that of tocopherol. Antioxidative effects of the selected medicinal methanolic extract on linoleic acid by thiocyanate acid method were examined for 15 days. Peroxidation of control and tocopherol group occurred on days 5 and 9, respectively, but BHA, BHT, selected medicinal methanolic extract group did not occur until on day 15. Antioxidative effects of the selected medicinal methanolic extract on linoleic acid by TBA method were examined for 15 days. Antioxidative activity was similar to those obtained by thiocyanate acid method.