국내 해조류 자원의 항염증 및 세포독성 스크리닝 평가

김철원,장광진,김연복,김동현,채철주,최한길,구현정,Kim, C.W.,Chang, K.J.,Kim, Y.B.,Kim, D.H.,Chae, C.J.,Choi, H.G.,Koo, H.J. 국립한국농수산대학교 교육개발센터 2020 현장농업연구지 = Journal of practical agricultural resear Vol.22 No.2

본 연구에서는 해조류 자원의 산업적 활용 소재로서의 가치 및 기능성 식품 및 사료 첨가 소재로의 개발을 위한 기초 자료를 제시하고자 국내에 서식하는 10종의 해조류 열수추출물을 이용하여 항염증 활성 및 마우스 대식세포주와 어류 세포주의 세포생존율에 미치는 영향을 확인하였다. 그 결과 S. japonica 100, 1000 ㎍/mL과 G. tenax 1000 ㎍/mL 농도에서 마우스 대식세포주를 LPS로 자극하였을 때 생성되는 NO를 유의적으로 감소시키는 것을 관찰하였으며, 나머지 8종의 해조류 추출물은 실험 농도에서 LPS에 의한 NO 생성에 영향을 미치지 않았다. 고농도에서 항염증 활성을 보인 S. japonica 및 G. tenax 열수추출물은 염증 인자를 조절할 수 있는 기능성 후보 소재로서 분획별 생리활성과 유효성분 규명의 연구가 요구된다. 본 연구 대상 해조류 추출물에 대한 안전성 기초 자료로서 마우스 대식세포 및 넙치 배아 유래 세포 세포생존율에 미치는 영향을 평가한 결과, P. tenera 고농도 (1000 ㎍/mL) 처리군을 제외하고 모든 해조류 추출물은 마우스 대식세포의 생존율에 영향을 주지 않았으며, 모든 해조류 추출물에서 5000 ㎍/mL까지 넙치 배아유래 세포에 대한 세포독성이 나타나지 않았다. 본 연구 결과는 후속 연구를 통해 해조류의 다양하고 복잡한 구조의 생물 활성 화합물의 생체 내 메커니즘을 규명함으로써 기능성 소재 개발을 위한 기초자로료 활용될 수 있을 것으로 기대하며, 본 연구의 대상 10종의 국내 해조류 자원 중 S. japonica과 G. tenax은 잠재적인 천연 항염증제로서 안전한 식품 및 사료 첨가 소재로서의 가능성을 제시한다. In this study, the anti-inflammatory and cytotoxic effects of hot-water extracts from 10 kinds of macroalgae in Korea were investigated. It was selected materials in consideration of biological activity and industrial potential as follows: Caulerpa okamurae; Codium fragile; Ulva australis; Ishige foliacea; Saccharina japonica; Sargassum horneri; Undaria pinnatifida; Gloiopeltis tenax; Gracilaria verrucosa; Porphyra tenera. Results showed that S. japonica and G. tenax significantly decreased NO productionn in LPS-stimulated Raw 264.7 cells at concentrations of 100, 1000 ㎍/mL and 1000 ㎍/mL, respectively. However, most of the other macroalgae used in the experiment did not affect NO production. It was observed that all macroalgae extracts except for the highest concentration (1000 ㎍/mL) treatment group of P. tenera did not affect the viability in Raw 264.7 cells. In addition, there was not significant decrease in cell viability by macroalgae extracts treatment in HINAE cells. These results suggest that S. japonica and G. tenax could be used as potential safe natural anti-inflammatory agents for food and feed additives. Also, the results of this study are expected to be used as basic data for the development of functional materials for 10 kinds of macroalgae resources in Korea.

왕우럭조개의 모패관리와 성숙촉진을 위한 가온 효과 구명

김철원,정달상,강한승,Kim, C.W.,Jeong, D.S.,Kang, H.S. 국립한국농수산대학교 교육개발센터 2019 현장농업연구지 = Journal of practical agricultural resear Vol.21 No.1

패류 인공종자생산에서 모패관리의 중요성이 커져가고 있으며 최적의 모패 관리 조건과 원하는 시기에 종자생산을 할 수 있는 성숙유도 조건을 구명하는 하는 것이 매우 중요하기 때문에 본 연구에서는 왕우럭 조개의 모패관리 최적조건과 성숙 촉진을 위한 가온 효과를 조사하였다. 모패관리는 실내사육에 비하여 실외사육이 비만도와 생존율 측면에서 더 효과적인 것으로 나타났으며 관리 측면에서도 인위적인 먹이생물 공급이 없으며 자연환경 조건에서 사육하기 때문에 관리가 수월하다는 장점이 있는 것으로 나타났다. 그리고 동계가온을 통한 성숙촉진 유도는 비만도와 생식소 관찰 등을 통하여 수온 18℃ 이상에서 2개월 정도 사육하였을 경우 성성숙이 이루어지는 것으로 나타나 성숙관리가 다른 패류에 비해 용이하다는 것을 알 수 있었다.

사육조건에 따른 왕우럭, Tresus keenae의 여수율과 먹이섭취률

김철원,정달상,Kim, C.W.,Jeong, D.S. 국립한국농수산대학교 교육개발센터 2012 현장농업연구지 = Journal of practical agricultural resear Vol.14 No.1

In juvenile size, the maximum clearance rate and ingestion rate were 15mm, which is the 70.8 mL/h, 4245.0×104cells/h, respectively. But the lowest clearance rate and ingestion rate were found at 5mm, which is the 4.1 mL/h, 246.0×104cells/h, respectively. The highest clearance rate and ingestion rate of AFDW seen at 7.5mm, with 3.3 mL/mg AFDW/h, 196.0×104cells/mg AFDW/h, respectively. According to the results of water temperature, clearance rate and ingestion rate were lowest at 10℃, showing the 0.3 mL/h, 20.3×104cells/h, respectively. The highest clearance rate and ingestion rate were 25℃, which is the 16.6 mL/h, 993.4×104cells/h, respectively. But in 30℃, clearance rate and ingestion rate decreased to the 12.9 mL/h, 772.6×104cells/h, respectively. With respect to the food density, the experiment groups with 30×104 cells/mL and 240×104 cells/mL showed the clearance rate of 21.1 mL/h and 2.3 mL/h, respectively. But the experimental group with 120×104 cells/mL or more showed decreased clearance rate. While, the maximum ingestion rate was at 60×104 cells/m, showing the 876.2×104cells/h.

육상수조에서 돌기해삼, Stichopus japonicus의 성장 및 생존율

김철원,허준욱,Kim, C.W.,Hur, J.W. 국립한국농수산대학교 교육개발센터 2018 현장농업연구지 = Journal of practical agricultural resear Vol.20 No.1

We assessed the effects of population density on the growth and survival rate of sea cucumber(Stichopus japonicus) in indoor tank Growth of S. japonicus in high density breeding was slow overall, and body weight on 250, 350 and 500 individual/m² group were 31.9g, 26.8g and 21.5g, respectively. The growth rate of body weight was 3.5~5.1 times according to the density of water, and the survival rate was 44.2~50.1%. The growth and survival rate were increased as decreasing the storage density. During the breeding season, small individuals tend not to be large and constantly culled, so it is necessary to set up the sorting period (etc.), and divide S. japonicus into groups of similar size.

담수어류 배가사리(Microphysogobio longidorsalis)의 서식 조건 평가

김철원,정달상,Kim, C.W.,Jeong, D.S. 국립한국농수산대학교 교육개발센터 2014 현장농업연구지 = Journal of practical agricultural resear Vol.16 No.1

We assessed the physical habitats of Microphysogobio longidorsalis in the Han river basin. Field monitoring was conducted for ecological and habitat conditions for 11 sites from October 2008 to November 2011. Twenty species (50.0%) including M. longidorsalis and Zacco koreanus were found endemic out of the 40 species in 10 families sampled during this study period. The most frequently found species was Z. platypus (26.2%) followed by Z. koreanus (17.7%), Coreoleuciscus splendidus (14.0%) and M. longidorsalis (13.4%). For M. longidorsalis, total fist length showing the highest number of samples were 8~10 cm (50.9%). the favored habitat conditions were estimated to be 0.4~0.5 m (56.1%) for water depth, 0.2~0.9 m/s (90.4%) for flow velocity, sand (0.1~1.0 mm)~cobbles (100.0~300.0 mm)(94.5%) for substrate size and run (60.2%) for habitat type, respectively.

먹이영양과 물만들기에 따른 붉은쏨뱅이(Sebastiscus tertius) 자어의 성장과 생존

김철원,정달상,Kim, C.W.,Jeong, D.S. 국립한국농수산대학교 교육개발센터 2011 현장농업연구지 = Journal of practical agricultural resear Vol.13 No.1

This study was performed to investigate the effects of food organism and water quality on the mass production of larval red marbled rockfish, Sebastiscus tertius. When rotifer cultured with different food stuffs were supplied to the larvae, rotifer cultured with concentrated Chlorella provided the highest in total length 11.9 mm and survival rate was the highest by 47.0% fed that cultured with ω-yeast Growth and survival rate of larvae fed on enriched rotifer with ω-yeast and super selco, super selco of enriched treatment showed that the best results not only growth but also survival rate. For instance of Artemia, the growth was not significantly different with other treatment but the survival rate was significantly higher than others. The growth of larvae fed with different diets indicated that rotifer+Artemia+commercial diet treatment was the highest on the total length and body weight 18.40 mm and 18.1 mg, respectively, whether the survival rate was rotifer+Artemia treatment was the highest as 57.5%. The most effective density of Chlorella was 10×10⁵ cells/ml for growth of larvae. That density showed that total length and body weight were 11.83 mm and 14.3 mg, respectively. The survival rate was the most effective for 20×10⁵ cells/ml of Chlorella density. That density represented the highest survival rate as 54.5%. Comparing of PSB adding effect, PSB density 15×10⁵ cells/ml was the most effective for growth of larvae and but for survival rate PSB density 10×10⁵ cells/ml was the highest as 61.0%.

하둡과 의미특징을 이용한 문서요약

김철원,Kim, Chul-Won 한국정보통신학회 2014 한국정보통신학회논문지 Vol.18 No.9

본 논문은 하둡 기반의 분산병렬처리에 의한 문서의 의미특징을 추출하고, 추출된 의미특징을 이용하여 문서를 요약하는 새로운 방법을 제안한다. 제안된 방법은 문서요약에 비음수 분해된 문서의 의미특징을 이용함으로써 문서의 내부 구조를 잘 표현 할 수 있다. 또한 하둡을 이용하여 빅데이터의 문서를 요약할 수 있다. 실험결과 제안방법이 단일 컴퓨터 환경에서 처리할 수 없는 대용량의 문서를 요약할 수 있음을 보인다. In this paper, we proposes a new document summarization method using the extracted semantic feature which the semantic feature is extracted by distributed parallel processing based Hadoop. The proposed method can well represent the inherent structure of documents using the semantic feature by the non-negative matrix factorization (NMF). In addition, it can summarize the big data document using Hadoop. The experimental results demonstrate that the proposed method can summarize the big data document which a single computer can not summarize those.

저온 소결용 Ni-ZN계 페라이트의 합성 및 전자기적 특성 연구

김철원,고재귀,Kim, Chul-Won,Koh, Jae-Gui 한국재료학회 2002 한국재료학회지 Vol.12 No.8

The Ni-Zn synthetic ferrite were acquired from thermally decomposing the metal nitrates Fe(NO$_3$)$_3$.$9H_2$O, Zn($NO_3$)$_2$.$6H_2$O, Ni($NO_3$)$_2$. $6H_2$O, and Cu($NO_3$)$_2$. $3H_2$O at $150^{\circ}C$ for 24 hours and was calcined at $500^{\circ}C$. Each of those was pulverized for 3, 6, 9, and 12 hours in a steel ball mill and was sintered between $700^{\circ}C$ and $1,000^{\circ}C$ for 1 hour, and then their microstructures and electromagnetic properties were examined. We could make the initial specimens chemically bonded in liquid at the temperature as low as $150^{\circ}C$, by using the melting points less than $200^{\circ}C$ of the metal nitrates instead of the mechanical ball milling, then narrowed a distance between the particles into a molecular level, and thus lowed sintering temperature by at least $200^{\circ}C$ to$ 300^{\circ}C$. Their initial permeability was 50 to 400 and their saturation magnetic induction density and coercive force 2,400 G and 0.3 Oe to 0.5 Oe each, which were similar to those of Ni- Zn ferrite synthesized in the conventional process. In the graph of initial permeability vs frequencies, we could observe a $180^{\circ}C$rotation of the magnetic domain, which appears in a broad band of microwave near the resonance frequency.

재래감귤 팔삭의 과피 추출물이 LPS로 활성화 된 RAW264.7 대식세포에서 염증매개물질 억제에 미치는 효과

김철원,김성무,정승원,김소미,안광석,Kim, Chul-Won,Kim, Sung-Moo,Jeong, Seung-Weon,K., So-Mi Cho,Ahn, Kwang-Seok 대한암한의학회 2011 大韓癌韓醫學會誌 Vol.16 No.2

Objectives : Citrus is the fruit that is readily available around us. Therefore, we investigated the anti-inflammatory effects of fraction isolated from the Citrus hassaku pericarp in RAW264.7 macrophage cells. Methods : The effects of fraction from Citrus hassaku pericarp on cell viability on RAW264.7 cells were measured by the MTT assay. The mRNA levels of iNOS and COX-2, its protein level by fraction of Citrus hassaku pericarp treatment in RAW264.7 macrophage cells were investigated by RT-PCR and immunoblots. Nitrite accumulation in the culture was measured colorimetrically by the Griess reaction using a Griess reagent. The amount of IL-6 and TNF-${\alpha}$ production was determined using an enzyme-linked immunosorbent assay (ELISA) kit. Results : The results indicated that the fraction of Citrus hassaku pericarp concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW264.7 cells. fraction of Citrus hassaku pericarp inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, fraction of Citrus hassaku pericarp suppressed the level of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with fraction of Citrus hassaku pericarp inhibited the production of IL-6 and TNF-${\alpha}$ in LPS-stimulated RAW264.7 cells. Conclusion : Our results indicate that fraction of Citrus hassaku pericarp potentially inhibits the biomarkers related to inflammation through the blocking of NF-${\kappa}B$ p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.

비음수 행렬 분해와 군집의 응집도를 이용한 문서군집

김철원,박선,Kim, Chul-Won,Park, Sun 한국정보통신학회 2009 한국정보통신학회논문지 Vol.13 No.12

Document clustering is an important method for document analysis and is used in many different information retrieval applications. This paper proposes a new document clustering model using the clustering method based NMF(non-negative matrix factorization) and refinement of documents in cluster by using coherence of cluster. The proposed method can improve the quality of document clustering because the re-assigned documents in cluster by using coherence of cluster based similarity between documents, the semantic feature matrix and the semantic variable matrix, which is used in document clustering, can represent an inherent structure of document set more well. The experimental results demonstrate appling the proposed method to document clustering methods achieves better performance than documents clustering methods. 문서군집은 정보검색의 많은 응용분야에 사용되는 중요한 문서 분석 방법이다. 본 논문은 비음수 행렬 분해 (NMF, non-negative matrix factorization)를 군집방법과 군집의 응집도(coherence of cluster)를 이용한 군집 내 문서들의 정제를 이용한 새로운 문서군집방법을 제안한다. 제안된 방법은 문서집합의 내부구조를 나타내는 의미특징행렬과 의미변수행렬 이용하여 문서군집의 성능을 높일 수 있고, 문장들 간의 유사도에 기반 한 군집의 응집도를 이용하여 군집내의 문서들을 정제하여서 재 할당함으로써 군집의 효율을 향상시킬 수 있다. 실험결과 제안방법을 적용한 문서군집방법이 다른 문서군집 방법에 비하여 좋은 성능을 보인다.