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광원의 종류에 따른 복합레진의 중합거동 및 중합률에 관한 연구
류주희,이인복,유현미,김미자,석창인,권혁춘 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.4
Objectives: The purpose of this study was to observe the reaction kinetics and the degree of polymerization of composite resins when cured by different light sources and to evaluate the effectiveness of the blue Light Emitting Diode Light Curing Units (LED LCUs) compared with conventional halogen LGUs. Materials and Methods: First, thermal analysis was performed by a differential scanning calorimeter(DSC). The LED LCU (Elipar Freelight, 320㎽/㎠) and the conventional halogen LCU (XL3000, 400㎽/㎠) were used in this study for curing three composite resins (SureFil, Z-250 and AEliteFLO). Second, the degree of conversion was obtained in the composite resins cured according to the above curing mode with a FTIR. Third, the measurements of depth of cure were carried out in accordance with ISO 4049 standards. Statistical analysis was performed by two-way ANOVA test at 95% levels of confidence and Duncan's procedure for multiple comparisons. Results: The heat of cure was not statistically different among the LCUs (p > 0.05). The composites cured by the LED (Exp) LCUs were statistically more slowly polymerized than by the halogen LCU and the LED (Std) LCU (p< 0.05). The composite resin groups cured by the LED (Exp) LGUs had significantly greater degree of conversion value than by the halogen LCU and the LED (Std) LCU (p = 0.0002). The composite resin groups cured by the LED (Std) LGUs showed significantly greater depth of cure value than by the halogen LCU and the LED (Exp) LGU (p < 0.05).
김창진,강병화,유인자,박동진,이현선,김영호,유익동 충남대학교 약학대학 의약품개발연구소 1996 藥學論文集 Vol.12 No.-
다양한 잡초로부터 유용 생리활성물질을 탐색하고자 46종 잡초의 methanol 추출액을 대상으로 50~100 ㎍/㎖ 농도로 실험한 결과, 항균 활성에 있어서는 파리풀, 까실쑥부쟁이, 중머리대가리, 큰엉겅퀴, 부처꽃 등이 antobleb 활성에 있어서는 낙지다리, 밭뚝외풀, 까실쑥부쟁이, 술패랭이꽃 등이 항암활성에 있어서는 파리풀, 골풀, 밭뚝외풀, 까실쑥부쟁이, 술패랭이꽃, 겨우살이 등이 항산화활성에 있어서는 골풀, 물레나물, 청비녀골풀, 금불초, 방울고랭이, 좀고추나물 등이 비교적 강한 활성을 나타내었다. (1996년 4월 12일 접수, 1996년 8월 8일 수리) To search for bioactive compounds from plant resources, 80% methanol extracts of 46 species of weeds were screened for their activities of antimicrobial, antioxidative, antiblebing, antitumor and herbicidal. Among extracts tested, some showed activities at the concentration of 50 to 100 ㎍/㎖. Phryma leptostachya var. asiatica, Aster ageratoides, Centipeda minima, Cirsium pendulum. Lythrum anceps showed antibacterial activity. Penthorum chinense, Lindernia procumbens, Aster ageratoides, Dianthus superbus var. longicalycinus showed antiblebing activity. Phyma leptostachya var. asiatica, Juncus effusus var. decipiens, Lindernia procumbens, Aster ageratoides, Dianthus superbus var. longicalycinus, Viscum album var. coloratum showed antitumor activity. Juncus effusus var. decipiens, Hypericum ascyron, Juncus papillosus, Inula britannicar var. chinensis, Scirpus wichurae, Hypericum laxum showed antioxidant activity.
Ryoo, In-Ja,Park, Hae-Ryong,Choo, Soo-Jin,Hwang, Ji-Hwan,Park, Young-Min,Bae, Ki-Hwan,Shin-Ya, Kazuo,Yoo, Ick-Dong Pharmaceutical Society of Japan 2006 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.29 No.4
<P>Glucose deprivation is a fundamental feature of poorly vascularized solid tumors and leads to activation of the molecular chaperone GRP78, which is associated with the unfolded protein response (UPR), a stress-signaling pathway, in tumor cells. We recently isolated an active compound, M126, that inhibits transcription from a GRP78 promoter reporter construct. M126 was identified as valinomycin by various spectroscopic methods. We found that valinomycin prevents UPR-induced protein expression, such as GRP78 and GRP94. The GRPs-inhibitory action of valinomycin severe hypoglycemic and results in selective cell death of the stressed cancer cells. Our findings demonstrate that GRP78 may be an excellent target for the use of cancer chemotherapy in the treatment of solid tumors.</P>
Kim, Sun-Ok,Sakchaisri, Krisada,Asami, Yukihiro,Ryoo, In-Ja,Choo, Soo-Jin,Yoo, Ick-Dong,Soung, Nak-Kyun,Kim, Young Sang,Jang, Jae-Hyuk,Kim, Bo Yeon,Ahn, Jong Seog American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.4
<P>The secondary metabolites illudins C<SUB>2</SUB> (<B>1</B>) and C<SUB>3</SUB> (<B>2</B>), obtained from the culture broth of <I>Coprinus atramentarius</I>, have been shown to possess antimicrobial activity. In the present study, we discovered novel biological activities of <B>1</B> and <B>2</B> in lipolysis of differentiated 3T3-L1 adipocytes and adipogenesis of 3T3-L1 preadipocytes. Compounds <B>1</B> and <B>2</B> exhibit a dose-dependent increase in glycerol release and thereby reduce intracellular lipid accumulation. The stimulatory effects of <B>1</B> and <B>2</B> on lipolysis are prevented by cAMP-dependent protein kinase (PKA) and extracellular signal-regulated kinase (ERK) inhibitors. Compounds <B>1</B> and <B>2</B> down-regulated perilipin and also affected the mRNA and protein levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL). However, <B>1</B> and <B>2</B> treatment leads to a significant increase in PKA-mediated phosphorylation of HSL at S563 and S660. In addition, <B>1</B> and <B>2</B> treatment in 3T3-L1 preadipocytes induces down-regulation of the critical transcription factors, CCAAT/enhancer binding protein α and β (C/EBPα and C/EBPβ), and peroxisome proliferator activated receptor γ (PPARγ), which are required for adipogenesis, and accordingly inhibits adipogenesis. These results suggest that <B>1</B> and <B>2</B> might be useful for treating obesity due to their modulatory effects on fat by affecting adipocyte differentiation and fat mobilization.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-4/np400520a/production/images/medium/np-2013-00520a_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np400520a'>ACS Electronic Supporting Info</A></P>
Kim, Dong-Seok,Lee, Hyun-Kyung,Park, Seo-Hyoung,Lee, Sangku,Ryoo, In-Ja,Kim, Won-Gon,Yoo, Ick-Dong,Na, Jung-Im,Kwon, Sun-Bang,Park, Kyoung-Chan Blackwell Publishing Ltd 2008 Experimental dermatology Vol.17 No.4
<P>Abstract: </P><P>Terrein, a fungal metabolite, has been recently shown to have a strong antiproliferative effect on skin equivalents. In the present study, we further investigated the effects of terrein on the possible signalling pathways involved in the growth inhibition of human epidermal keratinocytes by examining the regulations of extracellular signal-regulated protein kinase (ERK) and of the Akt pathway by terrein. It was observed that ERK was inactivated by terrein and that keratinocyte proliferation was inhibited, whereas Akt was unaffected. The inhibition of the ERK pathway by U0126 (a specific ERK inhibitor) also had a dose-dependent antiproliferative effect on human keratinocytes. These results indicate that ERK inhibition is involved in keratinocyte growth inhibition by terrein. Moreover, flow cytometric analysis showed that terrein inhibits DNA synthesis, as evidenced by a reduction in the S phase and an increase in the G<SUB>2</SUB>/M phase of the cell cycle. Thus, we next examined changes in the expressions of G<SUB>2</SUB>/M cell cycle-related proteins. Terrein was found to downregulate cyclin B<SUB>1</SUB> and Cdc2 without Cdc2 phosphorylation, but upregulated p27<SUP>KIP1</SUP> (p27), a known inhibitor of cyclin-dependent kinase. These results suggest that terrein reduces human keratinocyte proliferation by inhibiting ERK and by decreasing the expressions of cyclin B<SUB>1</SUB> and Cdc2 complex.</P>
Park, Seo-Hyoung,Kim, Dong-Seok,Lee, Hyun-Kyung,Kwon, Sun-Bang,Lee, Sangku,Ryoo, In-Ja,Kim, Won-Gon,Yoo, Ick-Dong,Park, Kyoung-Chan Blackwell Publishing Ltd 2009 Experimental dermatology Vol.18 No.6
<P>Abstract: </P><P>Previously, we reported that a fungal metabolite, terrein, decreases melanin synthesis via downregulation of microphthalmia-associated transcription factor (MITF). In the present study, we further investigated the long-term hypopigmenting action of terrein in a spontaneously immortalized mouse melanocyte cell line, Mel-Ab. Treatment with terrein at a concentration of 50 &mgr;<SMALL>M</SMALL> strongly decreased melanogenesis in a time-dependent manner. Interestingly, the decreased tyrosinase protein levels lasted for at least 7 days, even though the MITF protein levels were restored after 3 days of treatment. In accordance with the results of Western blot analyses, the tyrosinase mRNA levels were found to be continuously decreased for at least 7 days, even though recovery of the MITF mRNA levels began after 3 days of terrein treatment. Therefore, we evaluated tyrosinase downregulation to determine if it is caused by proteasomal degradation. We found that the reduction in tyrosinase levels that was induced by terrein was clearly recovered by MG-132, a proteasome inhibitor. Moreover, ubiquitination of tyrosinase increased following treatment with terrein in the presence of MG-132. Taken together, these results suggest that terrein decreases melanogenesis through ubiquitin-dependent proteasomal degradation as well as via decreased expression of its mRNA.</P>
( Dong Hyun Kim ),( Hye-min Kim ),( Pham Thi Thu Huong ),( Ho-jin Han ),( Joonsung Hwang ),( Hyunjoo Cha-molstad ),( Kyung Ho Lee ),( In-ja Ryoo ),( Kyoon Eon Kim ),( Yang Hoon Huh ),( Jong Seog Ahn ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.5
Methylation is a primary epigenetic mechanism regulating gene expression. 5-aza-2’-deoxycytidine is an FDA-approved drug prescribed for treatment of cancer by inhibiting DNA-Methyl-Transferase 1 (DNMT1). Results of this study suggest that prolonged treatment with 5-aza-2’-deoxycytidine could induce centrosome abnormalities in cancer cells and that CEP131, a centrosome protein, is regulated by DNMT1. Interestingly, cancer cell growth was attenuated in vitro and in vivo by inhibiting the expression of Cep131. Finally, Cep131-deficient cells were more sensitive to treatment with DNMT1 inhibitors. These findings suggest that Cep131 is a potential novel anti-cancer target. Agents that can inhibit this protein may be useful alone or in combination with DNMT1 inhibitors to treat cancer. [BMB Reports 2019; 52(5): 342-347]
Liu, Haidan,Hwang, Joonsung,Li, Wei,Choi, Tae Woong,Liu, Kangdong,Huang, Zunnan,Jang, Jae-Hyuk,Thimmegowda, N.R.,Lee, Ki Won,Ryoo, In-Ja,Ahn, Jong-Seog,Bode, Ann M.,Zhou, Xinmin,Yang, Yifeng,Erikson, American Association for Cancer Research 2014 CANCER PREVENTION RESEARCH Vol.7 No.1
<P>Mitogen- and stress-activated kinase 1 (MSK1) is a nuclear serine/threonine protein kinase that acts downstream of both extracellular signal-regulated kinases and p38 mitogen-activated protein kinase in response to stress or mitogenic extracellular stimuli. Increasing evidence has shown that MSK1 is closely associated with malignant transformation and cancer development. MSK1 should be an effective target for cancer chemoprevention and chemotherapy. However, very few MSK1 inhibitors, especially natural compounds, have been reported. We used virtual screening of a natural products database and the active conformation of the C-terminal kinase domain of MSK1 (PDB id 3KN) as the receptor structure to identify chrysin and its derivative, compound 69407, as inhibitors of MSK1. Compared with chrysin, compound 69407 more strongly inhibited proliferation and 12-<I>O</I>-tetradecanoylphorbol-13-acetate (TPA)-induced neoplastic transformation of JB6 P+ cells with lower cytotoxicity. Western blot data demonstrated that compound 69407 suppressed phosphorylation of the MSK1 downstream effector histone H3 in intact cells. Knocking down the expression of MSK1 effectively reduced the sensitivity of JB6 P+ cells to compound 69407. Moreover, topical treatment with compound 69407 before TPA application significantly reduced papilloma development in terms of number and size in a two-stage mouse skin carcinogenesis model. The reduction in papilloma development was accompanied by the inhibition of histone H3 phosphorylation at Ser10 in tumors extracted from mouse skin. The results indicated that compound 69407 exerts inhibitory effects on skin tumorigenesis by directly binding with MSK1 and attenuates the MSK1/histone H3 signaling pathway, which makes it an ideal chemopreventive agent against skin cancer. <I>Cancer Prev Res; 7(1); 74–85. ©2013 AACR</I>.</P>
Inhibitory Effect of Melanogenesis by 5-Pentyl-2-Furaldehyde Isolated from Clitocybe sp.
( Young Hee Kim ),( Soo Jin Choo ),( In Ja Ryoo ),( Bo Yeon Kim ),( Jong Seog Ahn ),( Ick Dong Yoo ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.6
In the continued search for melanogenesis inhibitors from microbial metabolites, we found that the culture broth of Clitocybe sp. MKACC 53267 inhibited melanogenesis in B16F10 melanoma cells. The active component was purified by solvent extraction, silica gel chromatography, Sephadex LH-20 column chromatography, and finally by preparative HPLC. Its structure was determined as 5- pentyl-2-furaldehyde on the basis of the UV, NMR, and MS spectroscopic analysis. The 5-pentyl-2-furaldehyde potently inhibited melanogenesis in B16F10 cells with an IC50 value of 8.4 μg/ml, without cytotoxicity.
Hydroxyhibiscone A, a Novel Human Neutrophil Elastase Inhibitor from Hibiscus syriacus
( Ryoo In Ja ),( Bong Sik Yun ),( In Kyoung Lee ),( Young Hee Kim ),( Ik Soo Lee ),( Jong Seok Ahn ),( Ki Hwan Bae ),( Ick Dong Yoo ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.8
In an ongoing investigation of compounds from natural products that exhibit anti-aging properties, hydroxyhibiscone A (1), a new furanosesquiterpenoid, together with hibiscone D (2), was isolated from the root bark of Hibiscus syriacus. Utilizing UV, IR, NMR, and MS spectroscopic analyses, these chemical structures were revealed. Compounds 1 and 2 were found to posses significant anti-aging properties on the human neutrophil elastase (HNE) assay, exhibiting HNE inhibitory activities with IC50 values of 5.2 and 4.6 μM, respectively.