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Ahn, Jinsook,Lee, Dukwon,Jo, Inseong,Jeong, Hyeongseop,Hyun, Jae-Kyung,Woo, Jae-Sung,Choi, Sang-Ho,Ha, Nam-Chul Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.3
Cryo-electron microscopy (cryo-EM) is now the first choice to determine the high-resolution structures of huge protein complexes. Grids with two-dimensional arrays of holes covered with a carbon film are typically used in cryo-EM. Although semi-automatic plungers are available, notable trial-and-error is still required to obtain a suitable grid specimen. Herein, we introduce a new method to obtain thin ice specimens using real-time measurement of the liquid amounts in cryo-EM grids. The grids for cryo-EM strongly diffracted laser light, and the diffraction intensity of each spot was measurable in real-time. The measured diffraction patterns represented the states of the liquid in the holes due to the curvature of the liquid around them. Using the diffraction patterns, the optimal time point for freezing the grids for cryo-EM was obtained in real-time. This development will help researchers rapidly determine high-resolution protein structures using the limited resource of cryo-EM instrument access.
( Sung-woo Ahn ),( Nam-joon Yi ),( Kyung Chul Yoon ),( Suk Kyun Hong ),( Hyo-sin Kim ),( Hyeyoung Kim ),( Youngrok Choi ),( Kwang-woong Lee ),( Kyung-suk Suh ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Purpose: Pediatric liver transplantation (PLT) has been the key therapy for end stage liver disease and the outcome has been excellent. However, still surgical complication associated with small recipient is the main cause of graft loss. In the present study, we assessed recent advances in outcome of PLTs through our experience. Methods: A total of 235 PLTs performed between Mar 1988 and July 2015 were analyzed. Three chronological periods were investigated: the initial period (1988-2007, n=117), the mid-term period in which our PLT management protocol was settled down (2008-2011, n=62), and the period in which surgical procedures were refined for small children (2012-2015, n=56). Results: The grafts’ (84% vs. 82% vs. 98%) and patients’ (84% vs. 87% vs. 98%) survival have been improved (p<0.05), whereas the number of biliary atresia (31% vs. 45% vs. 50%) deceased and the proportion of deceased donor (24% vs. 36% vs. 52%) and split PLT (7%vs. 16% vs. 46%) increased (p<0.05). The number of re-LT (3% vs. 9% vs. 2%) has been changed. ABO incompatible PLT has introduced on the last period (9%). Conclusion: The quality of the PLT has recently been standardized through a large volume of experience, and the operation has been proven to improve the survival outcome. However, a constant evaluation of our experience is critical for further progress.
Woo Heon Song,Jong Hyeok Park,Dong Woo Yeom,Byeong Kil Ahn,Kyung Min Lee,Sang Gon Lee,Hye Seung Woo,최영욱 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.1
A supersaturating self-emulsifying drug deliverysystem (S-SEDDS) was prepared and evaluated for enhanceddissolution of celecoxib (CXB), a poorly water-soluble drug. The selected CXB-dissolved SEDDS formulation consisting10 % Capryol 90 (oil), 45 % Tween 20 (surfactant), and45 % Tetraglycol (cosurfactant) had the characteristics ofsmall droplet size and great solubility as 208 nm and556.7 mg/mL in average, respectively. CXB dissolution fromSEDDS in simulated gastric fluid was increased to about20 % for the initial period of 5 min, but decreased to a halflevel as time elapsed. Thus, precipitation inhibitors werescreened to stabilize the supersaturation. The stabilizingeffect of Soluplus, an amphiphilic copolymer, was concentration-dependent, revealing the greatest dissolution ofapproximately 90 % level with delayed drug crystallizationby the addition of the copolymer. CXB dissolution fromS-SEDDS was pH-independent. We concluded that S-SEDDSformulation would be very useful in the future fordeveloping oral delivery product of poorly water-solubledrugs.
( Kyung Chul Yoon ),( Kwang-woong Lee ),( Hyeyoung Kim ),( Dongkyu Oh ),( Hyo-sin Kim ),( Sung Woo Ahn ),( Suk Kyun Hong ),( Nam-joon Yi ),( Kyung-suk Suh ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Liver transplantation(LT)has better outcomes even in patient with child A than LR because LT can overcome the tumor and background liver factors. We hypothesis that the according to the background liver and tumor biology, treatment option would be different. Methods: We reviewed patient data who received LR or LT in Seoul National university hospital. The patient was divided into two groups according to the platelet count which reflect the cirrhosis. We performed risk factor analysis using only preoperative risk factors. Platelet was used as a background liver factor(>100K=no or mild cirrhosis, ≤100K=moderate to severe cirrhosis)and AFP and tumor size and number is used as tumor characteristics. Results: Total 663 and 210 patient was included in LR and LT group. We compared overall survival(OS)and recurrence free survival(RFS)between LR and LT group according to platelet count. Three factors were used which was significant in multivariate analysis, AFP>100, the number of tumor>1, the size of tumor>5cm as a tumor factor. If no risk factors in platelet>100K group. RFS and OS were not different in both group. If one risk factors, OS was similar but RFS is higher in LT group. If 2risk factors, OS is higher in LT group. In platelet<100K group, if no risk, OS was similar in both group. But if 1,2risk factors, OS is higher in LT group. Conclusions: If no or mild liver cirrhosis, LR can be performed even the patient have 1 risk factor. But when the patient with moderate to severe cirrhosis, resection can be performed without any risk factor. LT have to be considered in patient with 2risk factors even the mild cirrhotic liver. And If the patient with moderate to severe cirrhosis, even the 1risk factor, transplantation have to be considered
Risk factors and Patterns of Extrahepatic Metastasis of Hepatocelluar Carcinoma
( Woo Jin Chung ),( Hong Sug Lee ),( Yoon Suk Hong ),( Min Kyung Kang ),( Byoung Kuk Jang ),( Kyung Sik Park ),( Kwang Bum Cho ),( Jae Seok Hwang ),( Sung Hoon Ahn ) 대한간학회 2006 Clinical and Molecular Hepatology(대한간학회지) Vol.12 No.3(S)
Differential Benefit of Statin 2ndary Prevention of AMI according to level of TG and HDL cholesterol
( Sang Woo Jeong ),( Wan Kim ),( Sun Ho Hwang ),( Won Yu Kang ),( Sang Cheol Cho ),( Kyung Hwan Kim ),( Gyu Ik Lee ),( Young Eun Jo ),( Myung Ho Jeong ),( Young Keum Ahn ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1
Background: The benefit of statin in acute myocardial infarction (MI) was well established. However, there were few studies about the differential efficacy of statin according to the baseline level of triglyceride and high-density lipoprotein (HDL)-cholesterol. Objective: To address the efficacy of statin in secondary prevention of MI according to the level of triglyceride and HDL-cholesterol on admission. Methods: The 36580 acute MI patients were enrolled from November 2005 to August 2012. Total patients were divided according to level of triglyceride and HDL cholesterol on admission and evaluated the efficacy of statin in patients with no lipid component of metabolic syndrome (Group A; n=15,461) (HDL cholesterol ≥40 mg/dL & triglyceride <150 mg/dL) and both components of metabolic syndrome (Group B; n=4,399)(HDL<0.001). However, the efficacy of statin was not prominent in Group B (HR=0.987; 95% CI; 0.708-1.376; p=0.939). After propensity matching, the survival analysis revealed that statin therapy reduced the risk of MACE in group A (HR=0.752, 95% CI; 0.609-0.929, p=0.008). This result was mainly due to reduction of cardiac death (HR=0.628, 95% CI; 0.420-0.938, p=0.023). Multivariate analysis revealed that use of statin contributed independently to improvement of clinical outcome in only Group A (HR=0.692, CI; 0.543-0.882, p=0.003). Conclusions: The long-term benefit ofstatin was not prominent in acute MI patients with lipid profiles of metabolic syndrome. Therefore, different lipid-lowering strategy is necessary in patients with low HDL and high triglyceride.
PE-137: Short Term Life Style Modification Can Improve Fatty Liver in Donor Candidates
( Kyung Chul Yoon ),( Kwang-woong Lee ),( Ok-kyung Kim ),( Sanghee Song ),( Suk Kyun Hong ),( Sung-woo Ahn ),( Hyo-sin Kim ),( Jin Yong Choi ),( Hyeyoung Kim ),( Nam-joon Yi ),( Kyung-suk Suh ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Purpose: It has been known that mild fatty liver grafts (<30%) do not have a significant impact on liver function and liver regeneration. However, NAFLD is related NASH and its general incidence has been reported upto 50% in general population. To reduce both donor and recipient``s risk, we have considered the donor``s liver with even mild fat fraction and inflammatory changes in preoperative liver biopsy. Methods: MRI is used for the decision for undergoing a biopsy and measuring of fat fraction. Even though fat fraction is mild, if it is greater than 5-10%, biopsy is undertaken considering the donor``s age and GRWR, the recipient``s liver function, and emergency status to check for NAFLD, NASH. If the above results are mild and the recipient``s status is not an emergency, we encouraged the donor to exercise. Follow up MRI and biopsy was done to check the changes in fat fraction and once again the recipient``s emergency status was checked to decide whether or not to proceed with the surgery. Moderate or severe degree of fat fraction were determined to be unsuitable for donation. Results: A total of 776 patients underwent donor workup for 588 patients waiting for liver transplantation between July of 2011 and June of 2015. A total of 65 patients had more than 5% fatty liver in CT or MRI, and from the 65 patients 19 patients had moderate to severe fatty liver and were determined to be unsuitable for donation. Out of the 45 patients who had mild fatty liver 13 patients`` biopsy showed more than 10% macrovesicular change and we encouraged life style modification for those 13 patients. Except only one patient, 12 patients were improved their fat fraction and biopsy result. About 45 to 119 days of life style modification by diet and exercise. these patients lost an average of 5.2 kg. Macrovesicular fatty change was reduced from 10-35% to 0-10% of these patients. Weight loss resulted mostly in reduction of macrovesicular change and also in reduction of inflammatory, fibrotic changes. Conclusion: In most of these patients about 2 to 3 months of life style modification resulting in weight loss lowered the fat fraction and changes of inflammation, fibrosis. Thus, it is important to educate and prepare the patients who are preparing for liver donation for liver transplantation.