ORiginal Article : N-Acetylcysteine Improves Pancreatic Microcirculation and Alleviates the Severity of Acute Necrotizing Pancreatitis

( Bing Qing Du ),( Yue Ming Yang ),( Yong Hua Chen ),( Xu Bao Liu ),( Gang Mai ) The Editorial Office of Gut and Liver 2013 Gut and Liver Vol.7 No.3

Background/Aims: To investigate the beneficial effect of N-Acetylcysteine (NAC) on pancreatic microvascular perfusion in acute necrotizing pancreatitis (ANP). Methods: Fifty-four rats were divided into a control group, an ANP group and an NAC-treated group. The ANP model was established by a retrograde injection of 3% sodium taurocholate into the pancreatic duct. The NAC-treated group received an intravenous infusion of NAC just 2 hours before and 30 minutes after the induction of ANP. The pancreatic microvascular perfusion was measured with laser Doppler flowmetry and pancreatic samples were collected for histological examination. Results: The microvascular perfusion in the NAC-treated group decreased slightly and exhibited a significant increase compared to the ANP group (p<0.01). A pathological examination revealed that edema and inflammatory infiltration decreased, and the hemorrhaging and necrosis of the pancreas were significantly reduced. Conclusions: NAC could improve pancreatic microvascular perfusion and alleviate the severity of sodium taurocholate-induced ANP, possibly representing a new therapeutic approach to prevent the progression of ANP. (Gut Liver 2013; 7:357-362)

Review on Kinematics Calibration Technology of Serial Robots

Chen-Gang,Li-Tong,Chu-Ming,Jia-Qing Xuan,Sun-Han Xu 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

Robot kinematics calibration is of great significance for improving robot absolute pose accuracy, which can be divided into modelbasedand non-parametric kinematics calibration. For model-based kinematics calibration, kinematics calibration modeling, posemeasurement of end-effector, kinematics parameters calibration and error compensation are systematically analyzed. And with theincreasing demand for autonomy, the autonomous kinematics calibration is introduced. On the other hand, research status of nonparametrickinematics calibration is elaborated, the advantages and disadvantage of which are discussed respectively. On the basis,comprehensive and organized description and summarization of the three kinds of calibrations are given. Overall, aiming at robotkinematics calibration, existing achievements are summarized, a typical calibration process is carried out and calibration precautionsare detailed, then calibration results of various methods are compared and analyzed. Finally, existing problems in kinematicscalibration are analyzed and development prospects are presented.

Effect of Variation of ABCB1 and ABCC3 Genotypes on the Survival of Bone Tumor Cases after Chemotherapy

Yang, Jie,Wang, Zhi-Gang,Cai, Hai-Qing,Li, Yu-Chan,Xu, Yun-Lan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

We conducted a comprehensive study to investigate the role of genes involved in transport pathways in response to chemotherapy and clinical outcome of osteosarcoma cases. Genotyping of six SNPs was performed in a 384-well plate format on the Sequenom MassARRAY platform for 208 osteosarcoma patients to reveal any correlations of the six SNPs with response to chemotherapy and clinical outcome. Individuals with the ABCB1 rs1128503 TT and ABCC3 rs4148416 TT genotypes had a higher probability of responding poorly to chemotherapy, indicated by odds ratios (ORs) of 2.46 (95%CI, 1.21-5.74) and 3.78 (95% CI, 1.20-13.85), respectively. Moreover, the ABCB1 rs1128503 TT and ABCC3 rs4148416 TT genotypes were significantly associated with shorter diseasefree survival (DFS) and overall survival (OS). Our study found the two SNPs in two transporter genes and one phase II metabolism enzyme to be associated with response to chemotherapy and overall survival in osteosarcoma patients, suggesting potential prognostic biomarker applications of the two SNPs.

A Novel Method for Survivability Test Based on End Nodes in Large Scale Network

( Ming Liang ),( Zhao Gang ),( Wang Dongxia ),( Huang Minhuan ),( Li Xiang ),( Miao Qing ),( Xu Fei ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.2

Survivability is a necessary property of network system in disturbed environment. Recovery ability is a key actor of survivability. This paper concludes network survivability into a novel composite metric, i.e. Network Recovery Degree (NRD). In order to measure this metric in quantity, a concept of Source-Destination Pair (SD Pair), is created to abstract end-to-end activity based on end nodes in network, and the quality of SD Pair is also used to describe network performance, such as connectivity, quality of service, link degree, and so on. After that, a Survivability Test method in large scale Network based on SD pairs, called STNSD, is provided. How to select SD Pairs effectively in large scale network is also provided. We set up simulation environment to validate the test method in a severe destroy scenario and evaluate the method scalability in different large scale network scenarios. Experiment and analysis shows that the metric NRD correctly reflects the effort of different survivability strategy, and the proposed test method STNSD has good scalability and can be used to test and evaluate quantitative survivability in large scale network.

Screening and Cloning of RAPD Markers from the W Chromosome of Silkworm, Bombyx mori L.

( Chun Xia Zhang ),( Qin Yao ),( Qing Gang Xu ),( Xu Dong Tang ),( Ke Ping Chen ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2

Silkworms sex determination drew high attention from researchers. Sex chromosomes on the silkworm are of ZW type for females and ZZ type for males. Chromosome W plays an important role in sex determination. Although several molecular linkage maps have been constructed for silkworm, very few markers are discovered on the W chromosome. In order to look for molecular markers and to further locate the Fem gene on chromosome W, we used genomic DNA from both female and male larvae of a silkworm strain named 937 as PCR templates for RAPD amplification with 200 arbitrary 10-mer primers. The amplification results showed three female-specific bands, namely OPG-07(496), OPC-15(1,660) and OPE-18(1,279). Further verification, however, revealed no band from OPG-07 and OPC-15 in either sex in the strain 798, but OPE-18 provided female-specific band in the strains Suluan7 and C108, and absent in both males and strain 798. This indicates that the bands from OPG-07(496) and OPC-15(1,660) are probably female-specific in strain 937, and the band from OPE-18 was probably amplified from a common segment shared by most strains. The genomic DNAs from OPG-07 and OPC-15 were cloned and sequenced. Sequence analysis showed that the DNAs from OPG-07 and OPC-15 have high identities with the retrotransposable elements, and DNA from OPC-15 contains a portion of sequence which probably encodes an eukaryotic translation initiation factor 4E binding protein (eIF4EBP).

Knockdown of HMGN5 Expression by RNA Interference Induces Cell Cycle Arrest in Human Lung Cancer Cells

Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.

Efficacy of lymph node dissection on stage IIICr of cervical cancer before CCRT: study protocol for a phase III, randomized controlled clinical trial (CQGOG0103)

Misi He,Mingfang Guo,Qi Zhou,Ying Tang,Lin Zhong,Qing Liu,Xiaomei Fan,Xiwa Zhao,Xiang Zhang,Gang Chen,Yuanming Shen,Qin Xu,Xiao-jun Chen,Yuancheng Li,Dongling Zou 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.3

Background: Cervical cancer is still present a major public health problem, especially in developing countries. In International Federation of Gynaecology and Obstetrics 2018, allowing assessment of retroperitoneal lymph nodes by imaging and/or pathological findings and, if deemed metastatic, the case is designated as stage IIIC (with r and p notations). Patients with lymph node metastases have lower overall survival (OS), progression free survival (PFS), and survival after recurrence, especially those who have unresectable macroscopical positive lymph nodes. Retrospective analysis suggests that there may be a benefit to debulking macroscopic nodes that would be otherwise difficult to sterilize with standard doses of radiation therapy. However, there are no prospective study reporting that resecting macroscopic nodes before concurrent chemoradiation therapy (CCRT) would improve PFS or OS of cervical cancer and no guidelines for surgical resection of bulky lymph nodes. The CQGOG0103 study is a prospective, multicenter and randomized controlled trial (RCT) evaluating lymph node dissection on stage IIICr of cervical cancer. Methods: Eligible patients are histologically confirmed cervical squamous cell carcinoma, adenocarcinoma, adeno-squamous cell carcinoma. Stage IIICr (confirmed by computed tomography [CT]/magnetic resonance imaging/positron emission tomography/CT) and the short diameter of image-positive lymph node ≥15 mm. 452 patients will be equally randomized to receive either CCRT (pelvic external-beam radiotherapy [EBRT]/extended-field EBRT + cisplatin [40 mg/m2] or carboplatin [the area under curve=2] every week for 5 cycles + brachytherapy) or open/minimally invasive pelvic and para-aortic lymph node dissection followed by CCRT. Randomization is stratified by status of para-aortic lymph node. The primary endpoint is PFS. Secondary endpoints are OS and surgical complications. A total of 452 patients will be enrolled from multiple hospitals in China within 4 years and followed up for 5 years.

Selective extraction of thorium to directly form self-assembly solid from HNO3 solution

Fang Zhang,Qiang Wu,Lei-Tao Sha,Yang Li,Xu-Xin Li,Ze-Yang Wang,Xuan Fu,Qing-Gang Huang,Bin Liu,Ze-Yi Yan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

Based on ions exchange between [DMDSA]+[Cl]- (Dimethyl distearyl ammonium chloride) and N,Ndialkyl-succinamide acid (SCA), three novel bifunctional [DMDSA]+[SCA]- ionic liquids (ILs) were firstlysynthesized for extraction of thorium (IV) by self-assembly strategy. The simultaneous extraction andsolidification of Th(IV) were unexpectedly realized in one-step operation using the present ILs in HNO3solution, and more than 99% thorium (IV) was enriched and immediately aggregated into selfassemblysolid at the biphasic interface. The self-assembly solid was further identified by FT-IR, SEM withelement mapping EDS and XPS analysis, and revealing that the self-assembly extraction (SAE) was triggeredby the amphiphilic [DMDSA]+ cations. A three-step extraction mechanism dominated by [SCATh(NO3)4]- was proposed based on the slope analysis method and HRMS analysis. The self-assembly extractionof Th(IV) exhibited the extremely excellent selectivity in the presence of U(VI) and typical lanthanideelements including La(III), Eu(III) and Lu(III), and the separation factors reached 2516 for Th/U, 1885 forTh/La, 1512 for Th/Eu and 558 for Th/Lu, respectively. The proposed SAE strategy was proved to be anefficient method for one-step separation and solidification of thorium ions from U(VI) and/or lanthanides.