Antimetastatic Effects of Capsaicin in Murine B16 Melanoma Cell Lines

Hwang Myung Sil,Yum Young Na,Kang Ho Il,Kim Ok Hee Korean Environmental Mutagen Society 2005 한국환경성돌연변이·발암원학회지 Vol.25 No.1

The present work was undertaken to evaluate the antimetastatic potential of capsaicin (8­methyl-N-vanillyl-6-nonenamide) by measuring its effects on matrix metalloproteinase activity, cell invasion and lung metastasis. Significant inhibition of matrix metalloproteinase-2 activity by capsaicin (100 $\mu$M) was detected by gelatin zymography. In vitro invasion assay showed capsaicin (50, 100 $\mu$M) reduced tumor cell invasion ($28-40\%$). Capsaicin (i.p., 2.5 mg/kg) inhibited development of lung colonization ($58\%$). These results suggest that capsaicin prevents metastasis in part through suppression of invasion of B16F10 melanoma cells by inhibiting matrix metalloproteinase-2 responsible for degradation of extracellular matrix.

The Risk Assessment of Heavy Metals for Herbal Medicine

HWANG Myung-Sil,KIM Hyun-Jeung,MIN Chung-Sik,PARK Gyung-A,ZE Keum-Ryon,YOON Eunkyung 大韓藥學會 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.1

Risk Assessment Principle for Engineered Nanotechnology in Food and Drug

Hwang, Myung-Sil,Lee, Eun-Ji,Kweon, Se-Young,Park, Mi-Sun,Jeong, Ji-Yoon,Um, Jun-Ho,Kim, Sun-Ah,Han, Bum-Suk,Lee, Kwang-Ho,Yoon, Hae-Jung Korean Society of ToxicologyKorea Environmental Mu 2012 Toxicological Research Vol.28 No.2

While the ability to develop nanomaterials and incorporate them into products is advancing rapidly worldwide, understanding of the potential health safety effects of nanomaterials has proceeded at a much slower pace. Since 2008, Korea Food and Drug Administration (KFDA) started an investigation to prepare "Strategic Action Plan" to evaluate safety and nano risk management associated with foods, drugs, medical devices and cosmetics using nano-scale materials. Although there are some studies related to potential risk of nanomaterials, physical-chemical characterization of nanomaterials is not clear yet and these do not offer enough information due to their limitations. Their uncertainties make it impossible to determine whether nanomaterials are actually hazardous to human. According to the above mention, we have some problems to conduct the human exposure risk assessment currently. On the other hand, uncertainty about safety may lead to polarized public debate and to businesses unwillingness for further nanotechnology investigation. Therefore, the criteria and methods to assess possible adverse effects of nanomaterials have been vigorously taken into consideration by many international organizations: the World Health Organization, the Organization for Economic and Commercial Development and the European Commission. The object of this study was to develop risk assessment principles for safety management of future nanoproducts and also to identify areas of research to strengthen risk assessment for nanomaterials. The research roadmaps which were proposed in this study will be helpful to fill up the current gaps in knowledge relevant nano risk assessment.

Application of Toxicogenomic Technology for the Improvement of Risk Assessment

Hwang, Myung-Sil,Yoon, Eun-Kyung,Kim, Ja-Young,Son, Bo-Kyung,Jang, Dong-Deuk,Yoo, Tae-Moo The Korean Society of Toxicogenomics and Toxicopro 2008 Molecular & cellular toxicology Vol.4 No.3

Recently, there has been scientific discussion on the utility of -omics techniques such as genomics, proteomics, and metabolomics within toxicological research and mechanism-based risk assessment. Toxicogenomics is a novel approach integrating the expression analysis of genes (genomic) or proteins (proteomic) with traditional toxicological methods. Since 1999, the toxicogenomic approach has been extensively applied for regulatory purposes in order to understand the potential toxic mechanisms that result from chemical compound exposures. Therefore, this article's purpose was to consider the utility of toxicogenomic profiles for improved risk assessment, explore the current limitations in applying toxicogenomics to regulation, and finally, to rationalize possible avenues to resolve some of the major challenges. Based on many recent works, the significant impact toxicogenomic techniques would have on human health risk assessment is better identification of toxicity pathways or mode-of-actions (MOAs). In addition, the application of toxicogenomics in risk assessment and regulation has proven to be cost effective in terms of screening unknown toxicants prior to more extensive and costly experimental evaluation. However, to maximize the utility of these techniques in regulation, researchers and regulators must resolve many parallel challenges with regard to data collection, integration, and interpretation. Furthermore, standard guidance has to be prepared for researchers and assessors on the scientifically appropriate use of toxicogenomic profiles in risk assessment. The National Institute of Toxicological Research (NITR) looks forward to an ongoing role as leader in addressing the challenges associated with the scientifically sound use of toxicogenomics data in risk assessment.

Egg formation in panoistic ovary of chrysomela populi and chryochus chinensis of coleoptera

Hwang, Myung Sil 慶北大學校 基礎科學硏究所 1992 慶北大學敎 基礎科學硏究所 硏究論文集 Vol.13 No.-

A novel anti-melanogenic agent, KDZ-001, inhibits tyrosinase enzymatic activity

Hwang, Kyu-Seok,Yang, Jung Yoon,Lee, Jooyun,Lee, Yu-Ri,Kim, Seong Soon,Kim, Geum Ran,Chae, Jin Sil,Ahn, Jin Hee,Shin, Dae-Seop,Choi, Tae-Young,Bae, Myung Ae Elsevier 2018 Journal of dermatological science Vol.89 No.2

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>The demand for anti-melanogenic agents is increasing due to the unwanted side effects of current treatments. To find an effective anti-melanogenic agent, we used zebrafish as a whole animal model for phenotype-based drug and cosmetic discovery screening.</P> <P><B>Objectives</B></P> <P>The aim of this study was to identify and explore a small molecule that could be used for skin-whitening cosmetics.</P> <P><B>Methods</B></P> <P>Using zebrafish embryos, we examined the effects of 1000 compounds on zebrafish development and pigmentation. Pigmentation production was assessed by tyrosinase (TYR) enzymatic activity and melanin contents. Pigmentation marker expression in the human melanoma cell line HMV-II was analyzed by western blot. We also tested reconstituted human skin tissue and analyzed KDZ-001 with computational molecular modeling.</P> <P><B>Results</B></P> <P>We identified three compounds that affected the pigmentation of developing melanophores in zebrafish. Among them, we identified KDZ-001, a novel anti-melanogenic agent, which strongly inhibits melanin synthesis in the developing melanophores of zebrafish, HMV-II cells, and reconstituted human skin with no toxicity. We found that KDZ-001 directly inhibits TYR enzymatic activity. Notably, computational molecular modeling of KDZ-001 suggested that its interaction with copper ions in the active site of TYR is essential for melanin synthesis, further demonstrating that KDZ-001 mainly acts as a TYR inhibitor to synthesize melanin.</P> <P><B>Conclusion</B></P> <P>KDZ-001 inhibits melanin synthesis and has a potential for use in skin-whitening cosmetics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In this study, we show that KDZ-001, a novel anti-melanogenic agent, strongly inhibits melanin synthesis in the developing melanophores of zebrafish, HMV-II cells, and reconstituted human skin with no toxicity. We also show that KDZ-001 directly inhibits TYR enzymatic activity. Thus, KDZ-001 is a potential candidate for a skin-whitening agent in the cosmetic industry. </LI> </UL> </P>

아리스톨로크산 함유 생약제에 대한 안전성평가연구 : 3개월간 반복투여독성시험을 통한 신장독성평가

황명실,박미선,문지영,이지선,염영나,이효민,신동환,강진석,윤은경,최미나,육미영,장동덕,길광섭,김승희,양기화 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 실험에서는 아리스톨로크산을 함운하고 있는 생약제중 하나인 마두령(.4risfoforfioe JTurruf)의 90일 반복투여독성시헌을 실f,」하였다. 마두령에서 아리스톨로크산을 정량분석한 결과 마두령 단일 건조븐말의 경운 2.112mg/g, 잉상에서 처방되고 있는 마든령복방 (마두령외 9가지 생약제 복합처방) 건조분말꼭 경무에는 0.066m9/5으로 각각 검출되었다. 본 실험에서 용량설정은 마두령 처리군 (저용량 군; 21.3m9/k9/day (임상용량), 중용량군; 2t3mgfg/day, 고용량군; 2430mg딘g b.w./day), 마두령복방 처리군 (427mg/kg b.w./da?; 임상용량) 및 positive control (아리스톨로크산) 처리군 (저용량군;0.05mgag/da17, 중용량군; 0.Smg/kg/day, 고용량군; 5mg./kg/dal·1으료 하여 랫드에게 경구투여하였다. 마두령단방 처궈근 및 마두령복방 처리군에서 저용량군과 중응량군에서는 잉상관찰 및 생화학적 분석에서 패조군과 유의적인 차이가 없었다. 그러나 마두령단방 고용량근에서는 간, 신장의 상대적인 무계가 증가되었고, 신장의 유두이행상피증식 및 암종이 관찰되었으며, 위에서는 편평세포암종이 관찰되었다. 하자만 간손상이나 간암은 유발하지는 않는 것으로 판단되었다. 결론끌으로 본 실험의 결과에서는 아리스톨로크산 함유 생약제인 마두령이 임상용량인 저응량 처리군에서는 독성을 나타내지 않았으나, 고용쏭으로 장기 복용시켰을 깅우 설치류의 전위부위 및 신장에서 독성이 있음을 알 수 있었다. Chines herbs nephropathy (CHN) has been described in young women who had taken a slimming pills containing some chines herbs. Aristolochic acid (AA), suspected substance as the causal factor of CHN, is known a carcinogen. The Aristolochiae fructus (fruit of Aristolochia contorta) was used in Korean Traditional Medicine consists of appropriate amounts of mixed natural products. Subchronic toxicity of A. furctus containing aristolochic acid was investigated in SD rats. The body weight and clinical signs were observed after orally administration of A. futctus at doses of 21.2, 213, and 2130 mg/kg/day; mixture (including 9 other herbs) at dose of 427 mg/kg/day; aristolochic aicd (Ⅰ+Ⅱ) at doses of 0.05, 0.5, and 5 mg/kg/day for 3 months. At the end of the treatment, high dose treated animals revealed a deficit in final body weight about 25% compared with that of control. Organ weights of kidney, liver, testis, or ovary were increased with dose dependent manner. No changes considered to be due to the administration of A. furctus, mixture or aristolochic acid were in hematological and clinical study. However, significant changes at histopathological study of kidney and stomach were boserved in high-dose treated groups. In conclusion, for human health safety it needs appropriate regulatory actions regarding the use of natural herbal medicines known or suspected of containing aristolochic acid.

Castleman disease of the abdomen

Myung-Hw Kim,Shin Hwang,Youn-Baik Choi,Sung-Tae Oh,Song-Cheol Kim,Eun-Sil Yu,Sung-Gyu Lee 대한외과학회 2010 대한외과학회 학술대회 초록집 Vol.2010 No.11

Association between Urinary Bisphenol A and Waist Circumference in Korean Adults

Ko, Ahra,Hwang, Myung-Sil,Park, Jae-Hong,Kang, Hui-Seung,Lee, Hee-Seok,Hong, Jin-Hwan Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.1

Bisphenol A (BPA) is widely used in the production of polycarbonate plastics, epoxy resins, and food and beverage containers. In the present study, we aimed to investigate the relationship between urinary concentrations of BPA and waist circumference in Korean adults. A total of 1,030 Korean adults (mean age, $44.3{\pm}14.6$ years) were enrolled in the study on the integrated exposure to hazardous materials for safety control, conducted by the Ministry of Food and Drug Safety from 2010 to 2012. Abdominal obesity was defined as having a waist circumference of at least 90 cm and 85 cm for men and women, respectively. The participants were divided into 4 groups according to the urinary BPA concentration quartile. Waist circumference was significantly higher among subjects with a urinary BPA concentration in the highest quartile relative to those in the lowest quartile (p = 0.0071). Linear regression analysis revealed a significant positive association between urinary BPA concentrations and body mass index, body fat, after adjusting for potential confounders. Moreover, subjects with urinary BPA concentrations in the fourth quartile were more likely to be obese compared to those with urinary BPA concentrations in the first quartile (odds ratio, 1.938; 95% CI: 1.314~2.857; p for trend = 0.0106). These findings provide evidence for a positive association between urinary BPA concentration and waist circumference in Korean adults.

기관지 천식환자에서 기도 투과성에 관한 연구

황정실 ( Jeong Sil Hwang ),김신애 ( Sin Ae Kim ),곽준구 ( Jun Gu Kwack ),박명재 ( Myung Jae Park ),어수택 ( Soo Taek Uh ),정연태 ( Yeon Tae Chung ),김용훈 ( Yong Hun Kim ),박춘식 ( Choon Sik Park ) 대한결핵 및 호흡기학회 1991 Tuberculosis and Respiratory Diseases Vol.38 No.2