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Spandana Rajendra Kopalli,차규민,Konkuk University,Sang-Ho Lee,Jong-Hwan Sung,Seok-Kyo Seo,Si-Kwan Kim 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2
Background: To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models. Methods: Hydrogen peroxide (H2O2)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting. Results: GINST treatment (50 mg/mL, 100 mg/mL, and 200 mg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 mM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 mg/mL and 100 mg/mL) significantly (p < 0.05) ameliorated the H2O2-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-a, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H2O2-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats. Conclusion: GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.
“Eye of the Spectator”: Surveillance and Fashion in The Spectator
( Kunng Eun Lo ),( Konkuk University ) 한국18세기영문학회 2013 18세기영문학 Vol.10 No.1
This Essay is primarily concerned with examining how fashion is inextricably up with discourses with vision and gender identity in The Spectator periodical. Whereas The Spectator has been commonly viewed in the context of bourgeois public sphere, this paper attempts to address issues of gender and fashion that have been largely neglected in Habermas`s accunt of the public sphere, This paper argues that Mr, Spectator`s surveillance of women as the proper objects of his visual and moral scrutiny is crucial because it functions as an imaginative tool to make sense of the realm of exchange and instability closely associated with expanding commerce. Accordingly, Mr, Spector`s persistent efforts to contain and manage excessive female consumption serve less to establish his scopic power than to reaffirm the powerful nature of female sexuality and the consuming desires of fashionable women who elude male surveillance through strategic display and deceptive appearances.
Structural assessment of the tetramerization domain and DNA-binding domain of CP2c
Ku-Sung Jo,Ki-Sung Ryu,Hee-Wan Yu,Seu-Na Lee,김지훈,Eun-Hee Kim,Konkuk University,김찬길,김철근,원형식 한국자기공명학회 2018 Journal of the Korean Magnetic Resonance Society Vol.22 No.4
Although the transcription factor CP2c has been recently validated as a promising target for development of novel anticancer therapy, its structure has not been solved yet. In the present study, the purified recombinant protein corresponding to the tetramerization domain of CP2c appeared to be well folded, whereas the Elf-1 domain showed a largely unfolded conformation. Particularly, the Elf-1 domain, which contains the putative DNA-binding region, showed a conformational equilibrium between relatively less-ordered and well-ordered conformers. Interestingly, addition of zinc shifted the equilibrium to the relatively more structured conformer, whereas zinc binding decreased the overall stability of the protein, leading to a promoted precipitation. Likewise, a dodecapeptide that has been suggested to bind to the Elf-1 domain also appeared to shift the conformational equilibrium and to destabilize the protein. These results constitute the first structural characterization of the CP2c domains and newly suggest that zinc ion might be involved in the conformational regulation of the protein.