비외상성 두개내 출혈 환자에서 심근손상의 발생과 예후에 미치는 영향

강구현,황성호,이강현,조준휘,김성환,문중범,박해상,이서영,이성수,김헌주 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Objective: The aim of this study was to investigate the clinical significance of myocardial injuries in patients with nontraumatic intracranial hemorrhage by identifying the occurrence of myocardial injury and defining its correlation with subsequent cardiovascular events. Subjects and methods: One hundred twenty-four patients with nontraumatic intracraninal hemorrhage presented to the emergency department within six hours from onset of symptoms were enrolled. Brain CT, serial electrocardiography, and echocardiography were done at the emergency center. Blood samples for troponin I and creatine kinase(CK)-MB were drawn immediately and eight hours after admission, Troponin I and CK-MB were measured using a chemiluminescent immunoassay, respectively. Results: Electrocardiographic and echocardiography abnormalities were found in 65 cases(52.4%) and 21 cases(17%), respectively. Serum troponin I and creative kinase-MB were increased in 35 cases (28.2%) and in 58 cases(46.8%), respectively. Abnormal findings of echocardiography and ECG, as well as elevated levels of serum troponin I and creative kinase-MB, were associated with an increased risk of cardiovascular event and survival. Logistic regression analysis revealed that an abnormal echcocardiographic finding and elevation of serum troponin I were factors associated with the occurrence an adverse cardiovascular event and that electrocardiographic abnormalities and initial mental status were factors associated with poor prognosis. Conclusion: This study reveals that actual myocardial injury develops in a significant proportion of patients with nontraumatic intracranial hemorrhage and that the development of the myocardial injury is associated with an adverse cardiovascular event that occurs during admission.

Abnormal texture evolution of rolled Mg–3Al–1Zn alloy containing initial {10-12} twins

Lee, Jeong Hun,Park, Sung Hyuk,Hong, Seong-Gu,Won, Jong Woo,Lee, Chong Soo Elsevier 2015 Scripta materialia Vol.99 No.-

<P>The importance of initial {10-12} twins in the formation of deformation texture was investigated by conducting strain path change tests of rolled Mg–3Al–1Zn alloy in which {10-12} twinning dominated the deformation. The existence of initial twins changed a twinning process by preferring the growth of initial twins rather than the nucleation of new twins, which led to a non-Schmid behavior of twinning characteristics and consequently caused a completely different deformation texture compared with the counterpart without initial twins.</P>

Glutamine (Q)‐peptide screening for transglutaminase reaction using mRNA display

Lee, Jae‐,Hun,Song, Changhyeon,Kim, Do‐,Hyun,Park, Il‐,Hyang,Lee, Sun‐,Gu,Lee, Yoon‐,Sik,Kim, Byung‐,Gee Wiley Subscription Services, Inc., A Wiley Company 2013 Biotechnology and bioengineering Vol.110 No.2

<P><B>Abstract</B></P><P>Information on subsite specificity of the transglutaminase (TG) is important to design any specific peptides for TG's applications and inhibitor studies. Here, mRNA display was introduced for identifying the subsite specificity of TG from <I>Streptomyces mobaraensis</I> (STG). Functionally active peptides expressed from mRNA display library were differentially conjugated to hexa lysine (K<SUB>6</SUB>)—beads according to their relative activities for STG. The active peptide substrates for STG were enriched through six rounds of screening, and its corresponding cDNA/mRNA sequences were identified by DNA sequencing. The results showed that tripeptides such as LQQ and TQP do not show any activity for STG, but the minimum size of the peptide displaying STG activity is pentapeptide. One such predicted peptide sequence, that is, RLQQP (TQ1), showed higher reactivity (ca. 182% conjugation yield) to STG than that of the highly active sequence, that is, control‐Q (PQPQLPYPQPQLPY), well‐known previously for mammalian TG2. Furthermore, when recombinant DsRed was tagged with TQ1 sequence at its C‐terminal, DsRed‐TQ1 underwent efficient covalent‐immobilization onto alginate–gelatin bead by STG reaction, showing a Q‐peptide application as a useful tagging molecule. Biotechnol. Bioeng. 2013; 110: 353–362. © 2012 Wiley Periodicals, Inc.</P>

Expression of Macrophage Colony-Stimulating Factor, Granulocyte Macrophage Colony-Stimulating Factor and Their Receptors in Ovaria

Lee, Hun Young,Ryu, Ki Sung,Kim, Jin Hong,Lee, Jong Kun,Kim, Jang Heub,Rha, Jong Gu,Han, Ku Taek,Kim, Soo Pyung CATHOLIC MEDICAL CENTER 1994 Bulletin of the Clinical Research Institute Vol.22 No.1

Colony-stimulating factors (CSFs) are a family of glycoprotein that are involved in the survival, proliferation, differentiation and activation of hematopoietic cells at various stages of their development. The clinical ramification and therapeutic evaulation of hematopoietic growth factors is a rapidly growing field that holds promise for the treatment of various medical illness. Many nonhematopoietic tumors produce hematopoietic growth factors that may influence cellular proliferation either by autocrine or by paracrine mechanism. So hematopoietic growth factors have been implicated in protean nohematopoietic process. In this study, expression of macrophage colony-stimulating factor (M-CSF), M-CSF receptor (M-CSF-R), granulocyte macrophage CSF (GM-CSF) and GM-CSF receptor (GM-CSF-R) was investigated in 37 gynecologic tissues including 31 samples of malignant ovarian tumor and 6 samples of benign ovarian tumor by RNA dot blot method. We systematically examined the relationship between the expression of CSFs and their receptors and clinical disease status. The results were as follows: 1. In 31 ovarian malignancies, RNA expression of CSFs and their receptors was detected from 15 cases (48.4%) in M-CSF, 20 (64.5%) in M-CSF-R, 13 (41.9%) in GM-CSF and 13 (41.9%) in CM-CSf-R. Co-expression of M-CSF and M-CSF-R was found in 13 cases (41.9%) and that of GM-CSf and GM-CSF-R in 10 cases (30.3%). In 6 benign ovarian cysts, GM-CSF and GM-CSF-R expressions were not detected, but M-CSF and M-CSF-R expressions were detected in one case, even though at very low intensity. 2. CA 125, the most significant tumor marker in ovarian cancer, was significantly elevated in the groups that expressed the M-CSF (P=0.047) and GM-CSF (P=0.045), but not in M-CSF-R and GM-CSF-R. 3. There were significantly high rates of RNA expression of M-CSf, M-CSF-R, GM-CSF and GM-CSF-R in poorly differentiated tumors (grade 3) compared with well and moderately differentiated tumors (P=0.001, P=0.035, P=0.003, P=0.005, respectively). By means of Mantel-Haenszel Chi-square analysis, the intensity of RNA expression also showed significant correlation with the histologic graded in M-CSF (P=0.005) and GM-CSf (P=0.041). 4. Age of patient, tumor size, pathologic diagnosis and FIGO stage did not show any signifcant relation with the expression of CSFs and their receptors. This results indicate that hematopietic growth factor modulate tumorigenesis by autocrine and paracrine mechanisms and the expression of hematopoietic growth factor and its receptor in ovarian malignancies could contribute to their proliferative and invasive characteristic in vivo.

Screening of cell-penetrating peptides using mRNA display.

Lee, Jae-Hun,Song, Hyun Seok,Park, Tai Hyun,Park, Tae Hyun,Lee, Sun-Gu,Kim, Byung-Gee Wiley 2012 Biotechnology journal Vol.7 No.3

<P>Cell-penetrating peptides (CPPs) are attractive vectors for in vivo and in vitro cellular uptake. Their use is, however, limited by insufficient understanding of their preference for a target cell. Here, a new CPP screening method is presented that uses mRNA display. After incubating the target cell lines, such as human embryonic kidney 293 (HEK 293) and HeLa cells, with an mRNA display library for 3 h at 37C, the CPP-mRNA nucleotide conjugates were harvested. These were amplified with PCR and subsequently sequenced. The screened CPPs for each cell line were identified after four rounds of selection. Among them, two peptides, MAMPGEPRRANVMAHKLEPASLQLR NSCA (CPPK) and MAPQRDTVGGRTTPPSWGPAKAQLRNSCA (CPPL) were selected, and the FITC-labeled peptides were evaluated for their ability to penetrate cells. The screened CPPs were superior to polyarginine (R(11) ), which is widely used as a standard peptide and shows good cell penetration efficiency. Our method can be applied to other target cells for which CPPs have not yet been elucidated.</P>

Experimental and numerical investigation of injection molding with microrib patterns

Lee, Jae Gu,Lee, Bong-Kee,Kang, Tae Gon,Kwon, Tai Hun Wiley Subscription Services, Inc., A Wiley Company 2010 Polymer engineering and science Vol.50 No.6

<P>In this study, experimental and numerical studies were carried out to investigate the transcriptability of microrib structures and flow characteristics in injection molding of such geometry. For this purpose, mold inserts with nine parallel microribs on top of a thick ground plate were designed and fabricated in such a way that the gate location could be varied to change flow directions: parallel or transverse to the microrib structures. By utilizing the fabricated mold insert, the transcriptability affected by gate location and flow direction was experimentally investigated. As for the numerical methods for injection molding filling simulation, we have developed an Interpolated Domain Decomposition Method (IDDM) enabling a coarse mesh for a ground plate and a finer mesh for microstructures. The effect of processing conditions on transcriptability has been extensively investigated. Under various processing conditions, an interesting flow phenomenon similar to “race-tracking” was observed due to “flow-hesitation” in the microribs. The same phenomenon was observed in flow analyses via IDDM, as well as a full 3D analysis. POLYM. ENG. SCI., 2010. © 2010 Society of Plastics Engineers</P>

Improving the growth rate of Escherichia coli DH5α at low temperature through engineering of GroEL/S chaperone system

Lee, Jae-Hun,Heo, Mi-Ae,Seo, Joo-Hyun,Kim, June-Hyung,Kim, Byung-Gee,Lee, Sun-Gu Wiley Subscription Services, Inc., A Wiley Company 2008 Biotechnology and bioengineering Vol.99 No.3

<P>GroEL/S is a molecular chaperone system in Escherichia coli which not only assists the folding of intracellular proteins but also affects the cellular activity against the change of environmental condition. Here we show that the growth rate of E. coli DH5α can be improved at low temperature by expressing a GroEL/S variant achieved through irrational protein engineering approach. The GroELS variant (GroELS<SUB>var</SUB>) accelerating the growth of E. coli DH5α was screened through enrichment culture of the mutant libraries obtained by random mutagenesis. E. coli DH5α harboring the groELS<SUB>var</SUB> gene exhibited approximately 1.5–2 times higher growth rate compared to the strain with wild-type GroELS at 15–30°C. At 10°C, a temperature that the growth of E. coli DH5α almost stops, the GroELS<SUB>var</SUB> triggered the growth of E. coli DH5α. We identified that seven nucleotides of groELS gene and six amino acids of the GroELS were changed through the mutagenesis and screening. Site directed mutagenic analysis revealed that H360 in GroEL<SUB>var</SUB> is the most crucial residue determining the activity of GroELS<SUB>var</SUB> and more than one of the other residues in GroEL<SUB>var</SUB> may be additionally involved in the activity of GroELS<SUB>var</SUB>. The improvement of growth rate induced by the GroELS<SUB>var</SUB> was observed only in the strain DH5α and not detected in other E. coli strains, such as BL21, BW25113, codon+, JM110, Top10, and XL1-blue. Biotechnol. Bioeng. 2008;99: 515–520. © 2007 Wiley Periodicals, Inc.</P>

Is inactivation of O⁶-methylguanine DNA methyltransferase still a favorable prognostic factor of patients with diffuse large B-cell lymphoma in the era of R-CHOP chemotherapy?

Lee, Gyeong-Won,Kang, Jung-Hun,Kim, In-Suk,Kim, Hoon-Gu,Ko, Gyung Hyuck,Lee, Jeong Hee,Kim, Dong Chool,Song, Dae Hyun,Yang, Jung Wook,Lee, Jong Sil Informa UK (TaylorFrancis) 2009 Leukemia & lymphoma Vol.50 No.12

Optimization of the enzymatic one pot reaction for the synthesis of uridine 5′-diphosphogalactose

Lee, Jae-Hun,Chung, Seung-Wook,Lee, Hwa-Jin,Jang, Kyuong-Soon,Lee, Sun-Gu,Kim, Byung-Gee Springer-Verlag 2010 BIOPROCESS AND BIOSYSTEMS ENGINEERING Vol.33 No.1

培養 脊髓 運動神經細胞에서 메틸수은에 對한 Vitamin E의 影響에 關한 硏究

李建穆,趙廷九,金仁淑,朴承澤,洪起年,螢根寧,李廷憲,徐銀俄,石勝瀚,趙光皓,崔珉圭,李昊燮,田炳薰,禹元洪,李康昌 대한동의병리학회 2000 동의생리병리학회지 Vol.14 No.1

메틸수은의 신경독성을 조사하기 의하여 생쥐의 배양 척수 운동신경세포를 여러 농도의 메틸수은에서 24시간 동안 배양한 후 MTT 분석법과 신경세사효소면역분석법으로 세독성을 분석하였으며, 또한 메틸수은에 신경세포에 대한 항산화제인 vitamin E의 방어효과를 조사하였다. 메틸수은은 처리한 농도와 시간에 비례하여 척수 운동세포의 생존율을 현저히 감소시켰다. 항산화제의 방어효과에 있어서는 vitamin E가 메틸수은에 의해서 유도된 신경독성을 방어하였다. 위의 결과로부터 메틸수은은 생쥐의 배양 척수 운동신경세포에 신경독성을 나타냈으며 vitamin E와 같은 선택적인 항산화제가 메틸수은의 독성을 방어하는데 매우 효과적이다. In order to evaluate the neurotoxic effect of methylmercuric chloride(MMC) on cultured mouse spinal motor neurons. cytotoxic effect was measured by MTT assay and neurofilament enzymeimmunoassay(EIA) after cultured mouse spinal motor neurons were incubated with various concentrations of MMC for 24 hours. The neuroprotective effect of antioxidant, vitamin E against MMC-mediated nurotoxicity was also examined in these cultures. MMC decreased cell viability of cultured mouse spinal motor neurons remarkably in a dose-and time-dependent manners. In protective effect of antioxidant, vitamin E was remarkably effective in blocking the neurotoxicity induced by MMC in MTT assay and neurofilament enzymeimmunoassay. From these results, it is regarded that MMC induce neurotoxicity, and the selective antioxidant such as vitamin E is very effective in blocking MMC-mediated neurotoxicity on cultured mouse spinal motor neurons