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Hou, Zhi-Hong,Zhao, Wen-Cui,Zhang, Qi,Zheng, Wei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5
Objective: To explore effects of paclitaxel-loaded poly lactic-co-glycolic acid (PLGA) particles on the viability of human hepatocellular carcinoma (HCC) HepG2 cells. Materials and Methods: The viability of HepG2 cells was assessed using MTT under different concentrations of prepared paclitaxel-loaded particles and paclitaxel (6.25, 12.5, 25, 50, and 100 mg/L), and apoptosis was analyzed using Hochest33342/Annexin V-FITC/PI combined with an IN Cell Analyzer 2000. Results: Paxlitaxel-loaded nanoparticles were characterized by narrow particle size distribution (158.6 nm average particle size). The survival rate of HepG2 cells exposed to paclitaxel-loaded PLGA particles decreased with the increase of concentration and time period (P<0.01 or P<0.05), the dose- and time-dependence indicating sustained release (P<0.05). Moreover, apoptosis of HepG2 cells was induced, again with an obvious dose- and time-effect relationship (P<0.05). Conclusions: Paclitaxel-loaded PLGA particles can inhibit the proliferation and induce the apoptosis of HCC HepG2 cells. This new-type of paclitaxel carrier body is easily made and has low cost, good nanoparticle characterization and sustained release. Hence, paclitaxel-loaded PLGA particles deserve to be widely popularized in the clinic.
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Hong-Man Hou,Dong-Qi Guo,Gong-Liang Zhang,Li-Ming Sun,Yu-Na Cui 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Cholesterol-lowering effect of lactic acid bacteria iswell-known. In the present study, nine cholesterol-loweringLactobacillus strains from Chinese traditional cheese, pickle, andyoghurt were screened and characterized for their potential use. The microbial contents of all strains significantly decreased at pH1.5; however, the residual counts of Lactobacillus casei subsp. casei GL-03, L. plantarum ZP-Z, L. plantarum ZP-05, and L. brevis ZP-04 were more than 107 CFU/mL after incubation for 6h. All nine strains of Lactobacillus indicated good tolerance to bileat concentration less than 0.2% after incubation for 2 to 6 h. L. plantarum ZP-W had maximum hydrophobicity towards xylene,whereas GL-03 strain possessed maximum hydrophobicity forboth hexadecane and octane. ZP-05 strain had more effectiveinhibitory activity against both Staphylococcus aureus and Bacillussubtilis than other eight strains. Furthermore, GL-03 strainsignificantly reduced cholesterol TC and TG levels in hyperlipidemiamice fed high-cholesterol diet. The growth of GL-03 strain waspromoted by five kinds of Chinese herbal medicines, and theChinese hawthorn at concentration of 0.0125% showed thehighest promoting effect. These results suggest that L. casei subsp. casei GL-03 may be effective as a probiotic with cholesterollowingactivities.
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Hou, Hong-Man,Guo, Dong-Qi,Zhang, Gong-Liang,Sun, Li-Ming,Cui, Yu-Na 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Cholesterol-lowering effect of lactic acid bacteria is well-known. In the present study, nine cholesterol-lowering Lactobacillus strains from Chinese traditional cheese, pickle, and yoghurt were screened and characterized for their potential use. The microbial contents of all strains significantly decreased at pH 1.5; however, the residual counts of Lactobacillus casei subsp. casei GL-03, L. plantarum ZP-Z, L. plantarum ZP-05, and L. brevis ZP-04 were more than $10^7CFU/mL$ after incubation for 6 h. All nine strains of Lactobacillus indicated good tolerance to bile at concentration less than 0.2% after incubation for 2 to 6 h. L. plantarum ZP-W had maximum hydrophobicity towards xylene, whereas GL-03 strain possessed maximum hydrophobicity for both hexadecane and octane. ZP-05 strain had more effective inhibitory activity against both Staphylococcus aureus and Bacillus subtilis than other eight strains. Furthermore, GL-03 strain significantly reduced cholesterol TC and TG levels in hyperlipidemia mice fed high-cholesterol diet. The growth of GL-03 strain was promoted by five kinds of Chinese herbal medicines, and the Chinese hawthorn at concentration of 0.0125% showed the highest promoting effect. These results suggest that L. casei subsp. casei GL-03 may be effective as a probiotic with cholesterol-lowering activities.
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Orientation-Dependent Hydration Structures at Yttria-Stabilized Cubic Zirconia Surfaces
Hou, Binyang,Kim, Seunghyun,Kim, Taeho,Park, Changyong,Bahn, Chi Bum,Kim, Jongjin,Hong, Seungbum,Lee, Su Yong,Kim, Ji Hyun American Chemical Society 2016 The Journal of Physical Chemistry Part C Vol.120 No.51
<P>Water interaction with surfaces is very important and plays key roles in many natural and technological processes. Because the experimental challenges that arise when studying the interaction water with specific crystalline surfaces, most studies on metal oxides have focused on powder samples, which averaged the interaction over different crystalline surfaces. As a result, studies on the crystal orientation-dependent interaction of water with metal oxides are rarely available in the literature. In this work, water adsorption at 8 mol % yttria-stabilized cubic single crystal zirconia (100) and (111) surfaces was studied in terms of interfacial hydration structures using high resolution X-ray reflectivity measurements. The interfacial electron density profiles derived from the structure factor analysis of the measured data show the existence of multiple layers of adsorbed water with additional peculiar metal adsorption near the oxide surfaces.Surface relaxation, depletion, and interaction between the adsorbed layers and bulk water are found to vary greatly between the two surfaces and are also different when compared to the previously studied (110) surface [Hou et al., Sci. Rep. 2016, 6, 27916]. The fractional ratio between chemisorbed and physisorbed water species were also quantitatively estimated, which turned out to vary dramatically from surface to surface. The result gives us a unique opportunity to reconsider the simplified 2:1 relation between chemisorption and physisorption, originally proposed by Morimoto et al. based on the adsorption isotherms of water on powder metal oxide samples.</P>
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기획주제(企劃主題) : 동아시아 한자(漢字),한자교육(漢字敎育)의 현황(現況)과 과제(課題); 漢字文化圈初級階段的詞彙與漢字敎學 -以瑞典隆德大學中文專業爲例
후홍옥 ( Hong Yu Hou ) 한국한자한문교육학회 2013 漢字 漢文敎育 Vol.30 No.-
스웨덴 룬드대학은 중국어 교육의 주요 기지이며, 중문과 전공의 초급 단계 학생들은 중국어 교육에 있어서 10년이 넘게 『初級漢語』라는 자신들이 편찬한 교재를 사용함으로써 학습 동기를 유발하고, 스웨덴어와 중국어를 서로 참고할 수있도록 하였다. 통계에 따르면, 『漢語水準詞彙與漢字等級大綱』의 갑급 단어와 비교해 본 결과, 약 41%의 갑급 단어가『初級漢語』에 보이지 않는다. 『初級漢語』의 단어가이렇게 된 이유는 한자의 출현 순서에 따라 본문의 내용을 따라갈 뿐, 『漢語水準詞彙與漢字等級大綱』의 단어 등급과 한자 등급을 따르지 않기 때문이다. 이외에도 전문적인 한자과목이 개설되지 않았기 때문에 학생들은 한자의 규칙을 학습하는데 매우 어려움을 느끼고 있으며, 이로 인해 학생들의 중국어 능력을 향상시키는데 일정 정도의 어려움이 있다. 본고는 비한자문화권 초급 단계 학생들에게 어휘와 한자를 교육하는 방법을 제시함으로써, 향후 비한자문화권 학생들의 중국어 학습에 있어 보다 효과적인 어휘와 한자 교육에 대해 토의하는 계기가 되고자 한다. Lund University is one of the important center of Chinese teaching in Sweden,the textbook Elementary Chinese used at the elementary stage is compiled by the teachers in the Chinese Department of this University,it is an interesting book with Sweden translation and used as long as more than ten years. According to statistics,compared with the Grade A vocabulary in the The outline of the Graded Vocabulary ,41% of the Grade A vocabulary doesn`t appear in Elementary Chinese,and apparently many words in Elementary Chinese are not included in The outline of the Graded Vocabulary.The order that the characters appear follows the content of the Text and isn`t based on The outline of the Graded Vocabulary. Furthermore, because there is no special Chinese characters courses,it is difficult for the students to learn the rule of Chinese character structure. Chinese characters become an obstacle for the students to promote their Chinese level. This paper tries to analyse how to teach Chinese characters for the students on the non circle of Chinese characters.
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Systemic Analysis on Laparoscope-Assisted Gastrectomy for Patients with Gastric Cancer
Hu, Jun-Hong,Tang, Hong-Na,Ma, Yong-Ping,Wang, Chen-Yu,Yao, Kun-Hou,Zhang, Jun-Jie,Ren, Xue-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5
Background: Laparoscope-assisted gastrectomy in treating patients with gastric cancers developed with a background of highly invasive traditional surgery and is being increasingly performed in the Asian Pacific area. This study systemically investigated the technique and clinical results for comparison with traditional radical subtotal gastrectomy for gastric cancers. Methods: Clinical studies evaluating the effectiveness and side effects of laparoscope-assisted gastrectomy in treating patients with gastric cancers were identified using a predefined search strategy. Summary rates of effectiveness and side effects of laparoscope-assisted gastrectomy were calculated. Results: Thirteen clinical studies which including 1,412 patients with gastric cancer treated by laparoscope-assisted gastrectomy were considered eligible for inclusion. Systemic analysis showed that, for all patients, the pooled resection rate was 100%. Major adverse effects were anastomotic stenosis, abdominal abscess, abdominal bleeding, postoperative ileus. Treatment related death occurred in 0. 71% (10/1412). Conclusion: This systemic analysis suggests that laparoscope-assisted gastrectomy in treating patients with gastric cancers is associated with good curative rate and acceptable complications.
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Hong Chang,Jiang Long Guo,Xiao Wei Fu,Xiu Jing Shen,Youming Hou,Kongming Wu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
Cryptochrome (CRYs) proteins have been elucidated as the molecular basis for magnetoreception in Drosophila, and a putative magnetic receptor (named IscA1) protein may aslo be involved in sensing magnetic fields in Drosophila. However, whether IscA1 has a conserved role in diverse animals and functions in orientation during animal migration is unknown. Here we report on the cloning and sequencing of the IscA1 gene from Agrotis segetum, which encodes a predicted protein IscA1 that has 131 amino acids and two conserved iron-sulphur cluster binding domains. Multiple sequence alignment and phylogenetic analysis were used to show that IscA1 had a relatively high homology from species of Noctuoidea. Quantitative polymerase chain reaction showed that IscA1 was ubiquitously expressed in adult organs and, among all developmental stages, expression was higher in adults. When Agrotis segetum was exposed to 14 h light/10 h dark, IscA1 expression also showed daily oscillations, and constant light or dark disturbed these oscillations. IscA1 expression levels in a migratory population were higher than in a reared population and higher in a southward migratory population than in a northward. These findings suggest that the IscA1 gene in A. segetum might be associated with migration and provide a molecular basis for further study on the functions of IscA1 gene in magnetoreception and potential control of the turnip moth.
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