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인삼 단백분획물이 일차배양한 계배의 근육세포에 미치는 영향
박미정(Mi Jung Park),송진호(Jin Ho Song),이흔파(Heum Pa Lee),김영중(Young Choong Kim) 한국생약학회 1990 생약학회지 Vol.21 No.3
Effects of the protein fraction of Panax ginseng on chicken embryonic skeletal muscle cells cultured with a deficient medium were studied. The protein fraction was further fractionated into four groups according to the molecular weight; larger than 10,000 dalton(fraction A), between 5,000 and 10,000 dalton(fraction B), between 1,000 and 5,000 dalton(fraction C), between 500 and 1, 000 dalton(fraction D). According to the microscopic observation, all four protein fractions at the concentration of 10∼100 ㎍/㎖ showed the tendency to stimulate the growth and differentiation of the muscle cells. The activity of acetylcholinesterase in muscle cells was significantly elevated by the protein fraction A at the concentration of 100 ㎍/㎖. Protein fractions B,C and D significantly enhanced the synthesis of RNA in the muscle cells. The synthesis of DNA in muscle cells was significantly enhanced by protein fractions A,B and C.
DNA/RNA Hybridization에 의한 흰쥐의 Transferrin mRNA 분포에 관한 연구
김세은(Se Eun Kim),김선여(Sun Yeou Kim),박미정(Mi Jung Park),송진호(Jin Ho Song),이은방(Eun Bang Lee),이흔파(Heun Pa Lee),김영중(Young Choong Kim) 대한약학회 1989 약학회지 Vol.33 No.5
Expression of transferrin gene in various organs of rat was studied using rat transferrin cDNA. The hybridization method of [35S]-labeled transferrin cDNA with transferrin mRNA in cytoplasmic preparations was used to measure the level of transferrin mRNA. The rat from 15-day old fetus to 21-day old postnatal were employed as an animal model. In the liver, the level of transferrin mRNA increased with increasing age. However, the level of transferrin mRNA in brain was significantly lower than that in liver and the level did not increase with age.