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박수일,방종득 한국어병학회 1994 한국어병학회지 Vol.7 No.2
넙치의 면역반응에 수온이 미치는 영향을 조사하기 위하여 넙치에 포르말린 사균 Edwardsiella tarda 로 주사면역시킨 후 수온변화에 따른 체액성면역반응을 관찰하였다. 저수온 (12, 15℃) 에서 항체는 면역 2∼3 주 후에 나타났으며 최대 항체가는 각각 16 과 32 이었다. 그러나 고수온(20, 23℃) 에서는 면역 1 주후에 나타났으며 최대 항체가는 약 2,048 로 높았다. 그리고 한번 형성된 항체는 수온의 변화에 민감하게 반응하였고 자연조건에서도 유사한 경향을 보였으며 면역지속기간은 19 개월 이상으로 나타났다. To investigate effects of water temperature on immune response of flounder, Paralichthys olivaceus, against Edwardsiella tarda, fish were immunized with formalin killed E. tarda antigen, and humoral immune response of these fish were observed. At lower water temperature (12 and 15℃), the antibody appeared 2 to 3 weeks after injection of formalin killed E. tarda antigen and the maximum agglutination titer was 16 and 32, respectively. However at higher water temperature (20 and 23℃), the antibody appeared one week after injection and the maximum agglutination titer was about 2,048. Once produced agglutination titer was sensitively responsed to variation of water temperature and showed that this phenomenon had also a similar tendency under natural condition. And it showed that agglatination titer of flounder immunized with formalin killed E. tarda maintained above 19 months.
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Gametogenesis, Gonadal Development and Maturation of the Sea Squirt, Halocynthia roretzi
김봉석,방종득,류호영,홍정표,정의영 한국발생생물학회 2001 발생과 생식 Vol.5 No.2
본 연구는 동해남부해역의 수하식 우렁쉥이 양식장에서 양식되고 있는 2년산 우렁쉥이, Halocynrhia roretzi의 생식세포 및 생식소 발달과 성숙 조사를 위하여 1996년 5월부터 1997년 4월에 걸쳐 조사되었다. 우렁쉥이는 자웅동체형으로 난생이었다. 난소는 체벽에 평행하게 붙어 좌측에 6~8개, 우측에 8~10개의 가는 생식수관을 가진 구조이며, 정소는 난소 사이에 불규칙 한 많은 낭상 구조를 가진 복합 생식소이었다. 난원세포는 직경이 11. Gametogenesis and gonadal development of the sea squirt Halocythia roretzi, which is two years old were investigated by histological study. The specimens were collected in Guryong-po coastal area Kyoungsangbuk-do, Korea from May 1996 to April 1997. The sea squirt is hermaphrodite and oviparous. The ovary is located in the inner wall of the tunic year-round, but the testis can be distinguished from in June. The ovary is composed of 6∼8 gonoducts at the left side and 8∼10 ones at the right side, the testis consists of the complex gonad having irregular sacular structures. Oogonia in the ovarian sac were 11.7∼15.6 in diameter. The early developing oocytes were 39.6∼47.6 and nucleus 10.0∼25.0 in diameter. Oocytes in the ovarian sacs during vitellogenesis were 158.6∼210.0 , and fully ripe oocytes which were to 210.0∼230.9 in diameter had several test cells in the cortical parts showing a characteristic of vertebrate. The testis showed a general spermatogenesis as in the marine animals. The three-year old sea squirt occurred the first spawning between January to February under 10
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박수일,방종득,박정우,이생동 한국어병학회 1992 한국어병학회지 Vol.5 No.2
발병한 양식 메기로 부터 vibrio속 균주를 분리하였다. 분리된 균주는 생물학적 생화학적 특성에 기초하여 동정한 결과 V. ordalii로 동정 되었다. 분리균은 glucose, lactose, maltose 와 salicine으로 부터 산을 생산 하였으며, arabinose, galactose, inocitol과 xylose는 이용하지 못하였다. 분리된 V. ordalii를 각각 KL-1, KL-2라 명명하였다. KL-1과 KL-2는 생리학적 특성이 유사하였다. 즉, pH 5-10, NaCl 0%∼6.0%에서 발육하였으며,또한 NaCl 7.0%, pH 10이상 그리고 pH5이하에서는 발육하지 않았다. KL-1균주를 건강간 메기에 인공 감염시킨 결과 양식장에서 발병된 증상과 동일한 출혈성 궤양이 유발되었다. 감염 24시간 후에 나타난 붉은 반점은 접종부위 주변에서 부터 확장되기 시작했으며, 감염 120시간 후부터는 배지느러미 부위까지 궤양이 확장되었다. 각각 온도에 따른 실험에서는 25℃에서 폐사율이 70%로 나타났다. 약제 감수성 시험에서 KL-1과 KL-2균주는 모두 GM, K, N, S와 SxT에 감수성을 나타내었으나, CF 및 L₂와 VA에는 저항성을 나타내었다. Two vibrio sp. strains were isolated from disease catfish(Silurus asotus). The present isolates were identified as Vibrio ordalii based on their biological and biochemical characteristics ; they were positive for acid production from glucose, lactose, maltose, sucrose and salicine, while negative for arabinose, galactose, inocitol and xylose. They are named KL-1 and KL-2. KL-1 and KL-2 strains were similar to physiological characteristics ; growth was observed at pH 5 to 10 and in 0% to 6.0% NaCl. Two strains did not growth at a concentration above 7.0% NaCl and pH10. This bacterium was injected into health catfish hypodermically. Such injection was found to induce haemorrhagic ulcers very similar to those observed in naturally infected fish. At 24h post-infection, the red spot developed around the injection site and grew bigger to from a red sport area. At 120h post-infection, the muscle ncerosis was extended near the ventral fin. The seventy percent lethal dosage was appeared to water temperature at 25℃. Two strains were tested for drug senistiveity by plate method. KL-1 and KL-2 strains were sensitive to GM. K N, S and SxT, but resistant to CF. L₂ and VA.
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전세규,정승희,방종득 한국어병학회 1990 한국어병학회지 Vol.3 No.1
방어의 類結節症 迅速診斷을 爲한 免疫螢光抗體法의 有用性을 檢討하기 위하여 原因菌으로 免疫된 抗血淸에서 免疫글로브린 G(IgG)를 分離하여 FITC標識 抗體를 精製하였다. 精製된 標識抗體는 螢光强度가 1 : 32였으며 이 標識抗體를 利用하여 1990年 7 月부터 10月까지 慶南 統營郡 所在 방어 養殖場을 對象으로 類結節症診斷試驗을 實施한 結果 直接螢光抗體法으로 2時間內에 原因菌의 迅速診斷이 可能하고 菌檢出面에서도 平板 塗抹 培養法보다 效科的인 것으로 나타났다. Yellow tail (Seriola quinqueradiata) infected by Pasteurella piscicida have been occurred to mass mortality without showing apparent surface lesions in cage culture farms. In this case, it is necessary to consider countermeasure by rapid diagnosis of infected fish. The purpose of the present study was to investigate usefulness of the direct fluorescent antibody technique(FAT) for rapid diagnosis of pseudotuberclosis of cultured yellowtail caused by P. piscicida. Antibody produced by inoculating rabbit with formalin killed pseudotuberclosis bacteria antigen(strain KNP-2). Immunoglobulin-G(IgG) was purified from antisera by DEAE-cellulose column chromatography and conjugate with fluorescein isothiocyanate. Fluorescein-labeled antisera was purified by sephadex G-25 gel column chromatography. The fluorescein/protein molar ratio of labeled antisera was determined as 8.8-9.5. Diagnosis of cultured yellowtail was examined in cage culture farms which located in Tongyung, kyungnam from July to October 1990. The causative bacteria of pseudotuberclosis could be detected within two hours after the specimens were transferred to the laboratory for FAT, and it showed that FAT could be adapted as a rapid and accurate diagnostic method of pseudotuberclosis in yellowtail.
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