관상 동맥 우회술 중 발생한 대동맥 박리증 -1예 보고-

박지권,양주민,김혁,김영학,강정호,정원상,김경수,신진호 대한흉부외과학회 2004 Journal of Chest Surgery (J Chest Surg) Vol.37 No.10

Development of intraoperative aortic dissection during coronary artery bypass surgery is extremely rare with catastrophic outcomes resulting in high rates of morbidity and mortality. The prognosis is highly dependant upon prompt diagnosis and emergent treatment. We report our experience on a 72 year old female patient who successfully received ascending aortic graft replacement for acute intraoperative aortic dissection during coronary artery bypass procedure. 관상 동맥 우회술 중 발생되는 수술 중 대동맥 박리증은 매우 드물며 높은 유병률 및 사망률을 가진 치명적인 질환이다. 이 질환의 예후는 신속한 진단 및 응급 처치에 좌우된다. 72세 여자 환자에서 관상 동맥 우회술 중 발생한 수술 중 급성 대동맥 박리증에 대해 인조 혈관을 이용한 상행 대동맥 치환술을 시행하여 좋은 결과를 얻었기에 보고하는 바이다.

산전선별검사(prenatal screening)의 최신 지견

박지권 대한산부인과학회 2017 연수강좌 Vol.57 No.-

Effect of shear on the Behavior of Polymer Melt Confined between Two Brushed Layers

박지권,김승현,조원호 한국고분자학회 2002 한국고분자학회 학술대회 연구논문 초록집 Vol.2002 No.4

Dynamic Properties of Polymer Melt in the Nanoscopically Confined Space

박지권,허준,조원호 한국고분자학회 2002 한국고분자학회 학술대회 연구논문 초록집 Vol.2002 No.10

노랑초파리 발생과정에서 sta 유전자의 발현 양상에 관한 연구

박지권,정형복,김세재 제주대학교 생명과학연구소 2000 제주생명과학연구 Vol.3 No.-

Drosophila stuvarista(sta) gene encodes the ribosomal protein D-p40, consisting of 270 amino acids. The D-p40 is highly conserved between such evolutionarily distinct organisms as human(63% identity) and hydra(58% identity) and has the effect of Minute phenotype such as other ribosomal proteins. We identified the sta gene in the process of Drosophila cDNA library screening and analyzed the developmental expression pattern of sta gene in the transcription level using in situ hybridization. Each animal stage and tissues of 3rd instar larva and adults were showed ubiquitously expression pattern. These results were similar to other ribosomal proteins that represented Minute phenotype. Also, we expressed the 31kDa recombinant proteins of D-p40 using his-tag expression system in E. coli and purified by affinity chromatography. Immunoblot result of the developmental stages was showedthat strongly detection only the stage of embryo and pupa. This result suggested that the expression of sta gene were regulated in the level of post-translation modification ailed considered that sta gene had to other function during development as well as to function as a core ribosomal component.

초자화동결된 생쥐 Preantral Follicle의 체외성장과 배란

박지권,백원영,Park, Ji-Kwon,Paik, Won Young 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.2

Objective: To define an appropriate vitrification condition of preantral follicle that yields high survival and to evaluate growth and ovulation rate of mouse follicles during in vitro culture after vitrification. Methods: Preantral follicles were isolated mechanically from mouse ovaries that were surgically recovered from mice aged 14 days. Retrieved preantral follicles were placed in EG (Ethylene Glycol) for 2, 5, 10 minutes and transferred to EFS-40 (40% EG, 18% Ficoll-70, 0.5 M sucrose) for 0.5, 1, 2 minutes. And then, preantral follicles were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing was carried out at room temperature. After defining the most appropriate vitrification condition that yields high survival, in vitro growth and ovulation rate of follicles were evaluated. Results: Appropriate vitrification condition that yield high survival rate ($83.2{\pm}2.1%$) of preantral follicle was EG for 5 minutes and EFS-40 for 0.5 minutes. In vitro survival rate of the vitrified preantral follicles were $85.5{\pm}0.5%$, $67.9{\pm}0.8%$ and $40.2{\pm}0.5%$ on day 2, 6 and 10. And in vitro growth of the vitrified preantral follicles were $107.1{\pm}16.1{\mu}m$, $117.1{\pm}18.4{\mu}m$, $178.4{\pm}45.6{\mu}m$ and $325.4{\pm}54.4{\mu}m$ on day 0, 2, 6 and 10. Although in vitro survival rate and growth of vitrified preantral follicles were lower than that of non-vitrified preantral follicles, the patterns of survival and growth were similar in vitrified and non-vitrified preantral follicles. The ovulation rate of antral follicles that was grown from vitrified preantral follicles was $32.6{\pm}1.2%$. Conclusion: Vitrified preantral follicles could be grown to antral sizes, and mature oocytes that can be used for IVF-ET programs were produced successfully. These data suggest that cryopreservation of preantral follicle by vitrification can be used for the preservation of the fertility.

모체태아의학 : OB-29 ; The modified Shirodkar procedure for rescue cervical cerclage: effective alternative to the McDonald suture

박지권,조인애,박지은,신정규,최원준,이순애,이종학,백원영 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: To evaluate the efficacy of the modified Shirodkar procedure for rescue cervical cerclage in our practice. 방법: Medical records of 55 cases of patients who underwent rescue cervical cerclage between 16?28 weeks gestation for cervical incompetence with cervical dilatation and membrane bulging at Gyeongsang National University Hospital, were reviewed for demographic and clinical data, gestation at cerclage, gestation at delivery, cerclage-delivery interval, and neonatal birth weight. Success was defined as the delivery of a baby who survived till discharge from hospital. 결과: Eighteen patients underwent McDonald cerclage and 37 patients underwent modified Shirodkar cerclage. Positive ROM test without gross ROM, the presence of membranes prolapsing into the vagina, and gestational age less than 24 weeks at cerclage placement are associated with failure of cerclage. There were no significant differences between McDonald and modified Shirodkar cerclage with respect to gestation at cerclage (23.2 (16?27.4) and 24.9 (16.6?28) weeks), gestation at delivery (29.3 (18.9?40.3) and 35.4 (17.9?40.7) weeks), rate of deliveries after 28 weeks (55.6 and 73.0%), after 32 weeks (44.4 and 67.6%), and after 36 weeks (33.3 and 48.6%), cerclage-to-delivery interval (9.6 (0?18.6) and 9.6 (0?19.6) weeks), neonatal birth weight (1425 (220-4180) and 2500 (200?5120) g), rate of cesarean delivery (27.8 and 35.1%) or success rate (66.7 and 78.4%). 결론: Modified Shirodkar cerclage is a safe and effective alternative to the McDonald approach in rescue cervical cerclage.

식도의 평활근종과 공존하는 표재성 식도암

박지권,이철범,전순호,김영학,정원상,김혁 대한흉부외과학회 2005 Journal of Chest Surgery (J Chest Surg) Vol.38 No.1

The coexistence of mesenchymal tumor and carcinoma in the esophagus is extremely rare. We report a case of squamous cell carcinoma located at the mucosal surface over leiomyoma of the esophagus. A 76-year-old man with complaints of 3 months onset of odynophagia was diagnosed preoperatively as squamous cell carcinoma over submucosal tumor with calcification. Esophagectomy and esophagogastrostomy were performed through the right thoracotomy and upper median laparotomy. The patient is doing well without evidence of recurrence in the 25 months after resection. We discuss the pathogenesis and possible relations between the two tumors. 식도에서 간엽세포 종양과 상피성세포 종양의 공존은 매우 드물다. 저자들은 식도 평활근종의 점막 표면에 위치한 편평상피세포암 수술 치험 1예를 보고한다. 내원 3개월 전부터 음식 섭취시 연하통을 주소로 내원한 76세 남자 환자에서 수술 전 검사로 석회화를 동반한 식도 점막하종양을 의심하였고, 종양을 덮고 있는 불규칙한 점막의 식도내시경 조직검사로 편평상피세포암의 공존을 수술 전에 진단하였다. 우측 개흉과 개복으로 흉부 식도절제술 후 식도-위 문합을 시행하였다. 수술 25개월이 경과한 현재까지 재발의 소견 없이 정상 생활을 하고 있다. 두 종양 사이의 관계의 가능성과 빈도에 대해 문헌 고찰을 한다.

난소암에서 면역조직화학법에 의한 14-3-3-sigma및 -zeta의 발현

박지권,신정규,이순애,이종학,최원준,백원영 대한부인종양학회 2005 Journal of Gynecologic Oncology Vol.16 No.2

Objective : To evaluate the expression level, localization and distribution of 14-3-3σ and ζ proteins in human ovarian epithelial carcinoma cells. Methods : Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and normal ovarian tissue (n=10) were analyzed. Goat anti 14-3-3σ polyclonal antibody and rabbit anti-14-3-3ζpolyclonal antibody were used as primary antibodies. Results : The 14-3-3σ protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3ζ protein expression in the glandular epithelium was significantly higher for cystadenocarcinoma than for cystadenoma and normal ovary. The 14-3-3σ and ζ proteins were immunohistochemically demonstrated in the nuclei and more preferably in the cytoplasm of adenocarcinoma epithelial cells. Conclusion : The expression of 14-3-3ζ protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3ζ protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell. 목적 : 부인암 중 발생빈도가 비교적 흔한 상피성 난소암에서 14-3-3ζ 단백과 14-3-3σ 단백의 발현 여부를 보고자 하였고 발현이 된다면 어떠한 양상으로 발현되는지, 그리고 조직 내에서의 발현은 어느 부위인지를 알아보고자 하였다. 연구 방법 : ○○대학병원 산부인과에서 자궁의 양성종양으로 수술하여 획득한 난소 중 조직병리학적으로 정상으로 판정된 10예의 난소조직과 난소종양으로 수술한 환자 중 조직병리학적으로 장액성 및 점액성 양성 및 악성종양으로 진단받은 조직 각각 10예를 대상으로 면역조직화학 염색법을 시행하여 관찰하였다. 결과 : 14-3-3σ 단백의 발현은 정상 난소보다 양성 상피종양세포에서 그리고, 양성 상피종양세포보다 악성 상피세포에서 증가되었으나 통계적으로는 유의하지 않았다. 14-3-3ζ 단백의 발현은 정상난소와 양성 종양상피세포에 비해 악성 종양상피세포에서 통계적으로 유의하게 증가되었다. 14-3-3 (σ,ζ) 단백의 발현은 장액성 및 점액성 악성종양 상피세포의 세포질과 핵에서 모두 관찰되었으며 핵에 비해 세포질 내에서 조금 강하게 관찰되었다. 결론 : 14-3-3ζ 단백은 상피성 난소암 발생에 있어 중요한 역할을 할 것으로 예측되며 이 단백의 발암과정에서의 보다 더 확실한 기능을 규명하기 위해서는 향후 이에 대한 추가 연구가 필요할 것으로 생각된다.

A clinical significance of emergency cerclage(초)

박지권,백종철,최원준,이순애,이종학,백원영 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-