Improving the quality of light-field data extracted from a hologram using deep learning

박대열,박중기 한국전자통신연구원 2024 ETRI Journal Vol.46 No.2

We propose a method to suppress the speckle noise and blur effects of the light field extracted from a hologram using a deep-learning technique. The light field can be extracted by bandpass filtering in the hologram's frequency domain. The extracted light field has reduced spatial resolution owing to the limited passband size of the bandpass filter and the blurring that occurs when the object is far from the hologram plane and also contains speckle noise caused by the random phase distribution of the three-dimensional object surface. These limitations degrade the reconstruction quality of the hologram resynthesized using the extracted light field. In the proposed method, a deep-learning model based on a generative adversarial network is designed to suppress speckle noise and blurring, resulting in improved quality of the light field extracted from the hologram. The model is trained using pairs of original two-dimensional images and their corresponding light-field data extracted from the complex field generated by the images. Validation of the proposed method is performed using light-field data extracted from holograms of objects with single and multiple depths and mesh-based computer-generated holograms.

인간 타액선세포 및 선암종세포주의 분화 유도시 Transglutaminase발현과 고정의존성 성장과의 관계

박대열,이선경,이화정,장현주,민승기,김은철 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

Transglutaminase(TGase) catalyzes the Ca^2+ dependent acyl transfer reaction to from γ-glutamyl lysine cross-links between substrate proteins. we have examined the expression of TGase 1 and TGase 2 enzyme activity and protein expression in differentiation induced salivary gland cells(HSG), and human salivary adenocarcinoma cell lines(SGT)in reation to anchorage independent growth. The TGase1 and TGase 2 activity in SGT was higher than in HSG, and there is no significant difference between TGase 2 and TGase1 cells. HSG cells showed no increase in TGase 1 and TGase 2 upon reaching post confluence and in 1.8mM Ca^2+. Expression of the TGase 1 and TGase 2 was induced by high cell density or by high calcium in SGT. No induction of TGase 1 & 2 protein was observed in postconfiuent HSG cells, but there was notable increases in TGase 1 & 2 protein in post confiuent SGT cells. This pattern of TGase 1 & 2 protein expression correlates with the pattern of particulate activity. SGT cells showed more colony size and colony forming activity in soft agar rather than HSG cells. The ability of SGT cells, containing high levels of TGase 2 and TGase1,to form colonies in soft agar suggests a role for this enzyme in anchorage independence.

현재 국내 가설교량 시장은 기존공법

박대열 한국발명진흥회 2003 發明特許 Vol.28 No.1

정상상태 연속 유동층 CVD 반응기에서의 입자크기 분포의 모사

박대원,한명완,윤풍,전종열,강신이 한국화학공학회 1987 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.25 No.4

본 연구는 정상상태의 연속 유동층 CVD 반응기내에서 실란의 열분해에 의한 고 순도 다결정 실리콘 제조에 관한 것으로 최적 조업조건 결정에 있어서 주입되는 실리콘 입자핵의 양이 반응기 내부에 존재하는 실리콘 입자 및 외부에 유출된든 실리콘 입자의 입도분포에 미치는 영향에 관하여 모델을 설정하고 모사를 실시하였다. 주입되는 실리콘 입자핵의 양이 감소하면 반응기 밖으로 유출되는 실리콘 제품의 생산량 및 반응기 내부와 유출 입자의 평균 입경은 증가하였으나 입자들의 입도분포가 넓어지는 경향을 나타내었다. 또한 정상상태 연속 조업실험 결과가 모사 결과와 잘 일치하여 모사 결과를 최적 조업조건의 결정에 이용할 수 있음을 입증하였다. A model was proposed to predict the particle size distribution in a steady state continuous CVD fluidized bed reactor and in outflow stream by variation of feed rate of input stream. In CVD fluidized bed reactor high purity polycrystalline silicon particles were grown by a pyrolysis of silane gas (SiH₄) and chemical vapor deposition (CVD) on seed particles. It was found from the computer simulation that the average size and output rate of product particles increased with decreasing silicon feed rate, and that the particle size distribution was broadened by the decrease of the feed rate, which is unfavorably influenced in a continuous CVD fluidized bed reactor. It was also demonstrated that the simulation results were in good agreement with experimental ones on a 4 inch CVD fluidized bed reactor and the model could be used in the estimation of operation conditions of CVD fluidized bed reactor.

Matrigel을 이용한 타액선세포의 삼차원적 배양효과

김석호,이선경,이화정,황영수,박대열,장현주,김은철 대한구강악안면병리학회 2003 대한구강악안면병리학회지 Vol.27 No.3

We used three-dimensional Matrigel culture system to examine the morphognesis of normal and malignant salivary glands cell in vitro including acinar cells(AC), myoepithelial cell(MC), salivary gland adenocarcinoma cells(SGT), mucoepidermoid carcinoma cells(MEC), and immortalized human salivary gland cells(HSG). For this purpose, normal and salivary gland tumor cells cultured in 3-D Matrigel, and characterized histologically and immunohistochemically, compared with same cells grown on monolayer culture and patient tissue from biopsy. 1. In three-dimensional Matrigel culture, HSG cells form acinar structure, SGT cells shows duct like structure, and other AC, MC and MEC cells dont' form any structure , and their morphology was different from that of monolayer cells. 2. Matrigel involved cell proliferation at a similar pattern to cells on plastic monolayer cell cultures, and monolayer cell revealed higher cell viability than that of Matrigel cultured cells. 3. All salivary glands cells on Matrigel or monolayer showed strong PCNA expression, and there is no expression difference in these cells. But some cells including myoepithelial cells in normal and salivary gland tumor tissue showing PCNA lavelling, so there is PCNA expression difference among normal and tumor tissue cells. 4. Actin expression was noted in AC cells on Matrigel, were rare expressed in the other cells except in MEC cells, and was present in myoepithelial cell and ductal cells of normal gland tissue. There is actin expression difference between tissue and cultured cells . 5. S-100 immunoreaction was moderateively positive in MC cells of monolayer culture, myoepithelial cells of normal tissue and pleomorphic adenoma, all cancer cells of mucoepidermoid carcinoma tissue, but significantly decreased in all salivary cells on Matrigel. 6. TGase 2 expression was prominent in MC cells of monolayer and Matrigel cultured, in myoepithelial cells of normal gland and pleomorphic adenoma, epidermoid cells of mucoepidermopid carcioma, and strong reaction in MEC and AC cells of monolayer and Matrigel cultured. 7. Expression of CK in monolayer culture showed strong reaction to CK6 in all sailvary gland cells, and mild reaction to CK10 and CK16 for all salivary cells, CK16 and CK19 expression in monolayer culture was similar to that of Matrigel culture. 8. CK6 and CK10 expression was strongest in AC and MC cells on Matrigel, and CK 4 was negative reaction in AC, SGT, MEC cells, strong reaction in MC cells but mild in SGT cells on Matrigel. Expression of CK was rare in HSG cells compared with other salivary gland cells, CK16 was prominent in SGT cells, CK10 and CK16 showed strongest expression in MEC cells of Matrigel. 9. Monolayer culture of HSG cell shwoing strong reaction to CK6, moderate to CK19 and mild to the others CK, but 3D cultured HSG cells reveal mild expression to CK16, and rare to others CK, intercallated duct in normal gland tissue showing strong to CK19, and mild to the others Ck, so there are CK expression difference in tissue, monolayer and 3-D cultured cells. 10. Monolayer culture of MEC cells represent strong reaction to CK6, mild to other CK, 3-D cells showing increased CK expression including CK6, epidermoid cells and intermediate cells in mucoepidermoid carcinoma tissue reveal positive to CK6 and CK16, mucous cell positive to CK10 and CK19, so Matrigel showed similar CK pattern compared to mucoepidermoid carcinoma tissue rather than monolThese data indicate that the interaction of salivary gland cells with basement membrane is an important factor in salivary gland development and cytodifferentiation, so this model system will be useful to study acinar or ductal differentiation in vitro.ayer cultred.

정상 및 HPV 불멸화 인간 구강상피의 삼차원적 배양

김성현,이선경,이화정,황영수,박대열,장현주,허용,김은철 대한구강악안면병리학회 2003 대한구강악안면병리학회지 Vol.27 No.4

Epithelial-mesenchymal interaction is well known to have an importance during the organ development as well as cell growth and differentiation. However, in vitro experimental model is not well developed to reproduce in vivo cellular micro-environment which provide a epithelial-mesenchymal interaction. The aims of this study were to develop and evaluate the in vitro experimental model that maintains epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally reconstituted human normal oral kertinocyte(NHOK) and immortalized human oral keratinocytes(IHOK) by histological and immunohistochemical analysis. The results were as follows; 1. Best condition of three dimensionally reconstituted IHOK & HaCaT cells are 14 days air-exposure cultivation, 3 days of submerged state, and dermal equivalent consisting type I collagen and IGF cells. 2. In comparison to IHOK, there was better preservation of the overall epidermal strucutures in oral cracioma cells (HN30) & HaCaT cells by organotypic cultures. But orgnaotypic co-culture of the normal keratinocyte showed the thinnest epithelial layer formation. 3. PCNA was detected primarily in the basal layer of normal mucosa and NHOK, whereas was shown throught the epithellium except surface layer of IHOK cells, and it's expression was similar to that of CIS of biopsied patient's tissue 4. Involucrin is expressed in the upper layer of oral mucosa and NHOK raft, but staining for involucrin was induced in the IHOK rafts indicating differentiation is incomplete, and the staining pattern in the IHOK raft was not uniform. 5. Normal oral keratinocyte raft showed weak immunostaining for p53, and p53 expression of IHOK raft increased rahter than in NHOK. In organotypic cultures of normal cells and IHOK, p53 expression was restricted to the proliferative part of epithelium. This is consistent with expression pattern in biopsy specimens of the normal and CIS tissue. 6. In artifically reconstructed NHOK, the pattern of keratin staining showed both similarity and differences from that of intact normal mucosa. An obvious difference was increased expression of CK10 & CK19, and decreased expression of CK6 in a reconstructed NHOK rather than in normal mucosa, and similar expression was in CK4 and CK16. 7. CK19 & CK16 were strongly positive in HPV immortlaized keratinocyte rafts rahter than in NHOK, an indicator of premalignant or malignant changes, while CK10 & CK6 were decreased, and organotypic cultures of IHOK express was similar keratin expression as epithelial dysplasia or CIS tissue. These results suggest that three-dimensional organotypic co-culture of normal oral & immortalized keratinocytes with dermal equivalent consisting type I collagen and fibroblasts results in similar morphologic and immunohistochemical characteristics to in vivo patient specimens. Thus this organotypic system can be used for studing mechanism of epitehlial-mesenchymal interaction in particular regulating epidermal diffenentiation and morphogenes

현장실험을 통한 외부 후긴장 Steel l-Beam의 휨 거동 특성

조두용,박대열,박선규 한국구조물진단유지관리공학회 2002 한국구조물진단유지관리공학회 논문집 Vol.6 No.3

고양이의 자궁샘근육증

허형문,정지열,강상철,박대식,배종희,김재훈 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.1

Adenomyosis is a nonneoplastic proliferation of uterine glands characterized by multicentric infiltration ofendometrial tissues into the myometrium. A female domestic short hair cat with unknown age was refered to localanimal hospital in Busan for ovariohysterectomy to prevent the unwanted offspring. At the time of surgery, the uteruswas enlarged with multiple nodular protrusion on the serosa. On the cut surface of uterus, elevation of the endometriumand hypertrophied myometrium were observed. Microscopically, the uterus was characterized by severe proliferationof endometrial glands into the lumen and within the myometrium. The intra-myometrial endometrium is circum-ferentially surounded by bundles of hypertrophic smooth muscle cells. These endometrial glands had tall columnarepithelium lacking nuclear atypia and mitoses. In our best knowledge, this is the first report of feline adenomyosisin Korea.

타이바의 풀림 특성에 관한 연구

배봉국,박홍선,석창성,박대열 한국정밀공학회 2005 한국정밀공학회 학술발표대회 논문집 Vol.2005 No.5월

초등학교 여학생의 체육수업 적응 경험

박창범(Park Chang-Beom),박대권(Park Dae-Gwon),이사열(Lee Sa-Yeol) 한국체육과학회 2008 한국체육과학회지 Vol.17 No.2

The purpose of this study was to investigate the adaptation processes of the low-skilled female elementary school student. To achieve this purpose, the eight female elementary students of the D elementary school were selected as participants. The data were collected by in-depth interviews. From the results of female students' interviews via the grounded theory-paradigm presented by Straus & Corbin(2001), 80 concepts and 18 subordinate categories, 12 categories were derived. First, the heart matter was 'difficulty' in physical education lessons. Second, the heart matters, 'difficulty', were various according to individual characters, secondary sex condition, laborious life in physical education lessons, troubles in social relationships, discontent in the surroundings of physical education lessons. Third, to solve the heart matter, the participants were using passive strategies such as 'abandonment', 'feign illness and lie' and positive strategies such as 'appeal to others', 'efforts'. Fourth, the helpful conditions in these strategies were 'participants' will', 'aids of others'.