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        대장균에서 무작위 샤인-달가노 서열이 소성장호르몬 유전자 발현에 미치는 영향

        나경수,나경수,백형석,이용세 한국생명과학회 2000 생명과학회지 Vol.10 No.4

        In order to search for the effects of Shine-Dalgarno (SD) sequence and nucleotide sequence of spacer region (SD-ATG) on bGH expression, oligonucleotides containing random SD sequences and a spacer region were chemically synthesized. The distance between SD region and initiation codon (ATG) was fixed to 9 nucleotides in length. The expression vectors have been constructed using pT7-1 vector containing a T7 promoter. Positive clones were screened with colony hybridization and named pT7A or pT7B plasmid series. The selected clones were confirmed by DNA sequencing and finally, 19 clones having various SD combinations were obtained. When bovine growth hormone was induced by IPTG in E. coli BL21(DE3), all cells harboring these plasmids produced a detectable level of bGH in western blot analysis. However, various SD sequences did not affect on bGH expression, indicating that the sequences of SD and the spacer region did not sufficiently destabilize mRNA secondary structure of bGH gene. Therefore, these results indicate that the disruption of mRNA secondary structure might be a major factor for regulating bGH expression in the translational initiation process.

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        Osteomas of the craniofacial region

        나경수 대한영상치의학회 2011 Imaging Science in Dentistry Vol.41 No.3

        Purpose : The purpose of this study was to present the clinical features of a case series of osteomas in the craniofacial region and to compare them with those described in the dental literatures. Materials and Methods : A retrospective study of 18 patients diagnosed with osteomas in the craniofacial region was performed. The age, gender, location, symptoms, and the radiological findings were recorded. Results : There were 13 women and 5 men from 18 years to 69 years of age (mean age, 42±27 years). Fourteen osteomas were found in the mandible (78%), two in frontal sinus, one in sphenoid bone, and one in maxilla. Conclusion : Osteomas are benign tumors composed of mature compact bone or cancellous bone. They are essentially restricted to the craniofacial skeleton and rarely, if ever, are diagnosed in other bones. Purpose : The purpose of this study was to present the clinical features of a case series of osteomas in the craniofacial region and to compare them with those described in the dental literatures. Materials and Methods : A retrospective study of 18 patients diagnosed with osteomas in the craniofacial region was performed. The age, gender, location, symptoms, and the radiological findings were recorded. Results : There were 13 women and 5 men from 18 years to 69 years of age (mean age, 42±27 years). Fourteen osteomas were found in the mandible (78%), two in frontal sinus, one in sphenoid bone, and one in maxilla. Conclusion : Osteomas are benign tumors composed of mature compact bone or cancellous bone. They are essentially restricted to the craniofacial skeleton and rarely, if ever, are diagnosed in other bones.

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      • KCI등재

        아담신화와 무위자연론의 비교 : 문화의 개념 정립을 위한 시고

        羅景洙 全南大學校 人文科學硏究所 1993 용봉인문논총 Vol.22 No.-

        A common feature that we can find out from comparing the Myth of Adam with the Doctrine of Inactive Nature is that both have a same idea about Knowledge Both the Garden of Eden and the World of Inactive Nature are places where Knowledge connot maintain its position. On the contrary, the Lost Paradise and the earthly world are places where we seek after Knowledge, and are supported by Knowledge. Lao-tzu said that the best goodness is letting matters their natural courses. That idea is comparable to the idea of Myth of Adam: 'We can live permanently only by not eating the fruit of the Tree of Knowledge' The Lost Paradies is a cultural life or cultural place in the Myth of Adam, because it is the place where we must work and produce something useful. That is to say, the fruit of the Tree of Life is a symbol of permanent life, and the fruit of the Tree of Knowledge is a symbol of Knowledge. So we can conclude that the places, where we can be workless and unproductive, is the world of Inactive Nature, while the place where we must work and produce something is artificial and cultural world.

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        온라인 환경 하에서 제품가격의 동적인 변화에 대한 탐색적 연구

        나경수,손영석 한국콘텐츠학회 2020 한국콘텐츠학회논문지 Vol.20 No.2

        본 연구는 온라인상에서 판매되는 동일한 제품들이 각각 다른 가격으로 판매되는 현상에 영향을 조망하는데 연구의 목적이 있다. 이를 위해서 온라인상에서 가격 분산에 대한 영향을 미치는 4가지의 요인을 중심으로 탐색적인 연구를 진행하였다. 첫째로 제품의 고유 속성인 제품의 평균가격, 최고가격, 최저가격, 둘째로 시장요인인 출시연도, 출시개월 수, 공급업체 수 셋째로는 소비자요인인 해당 제품에 대한 리뷰수와 평가태도 등의 요인들이 가격분산에 어떤 영향을 미치는지 파악하는 것을 본 연구의 초점으로 설정하였다. 연구의 분석을 위해 온라인의 가격비교 사이트 중에서 대표적인 서비스인 다나와를 통해서 대상제품군을 선정하였다. 먼저 제품군을 제품 컨셉에 따라서 효용적 개념의 제품과 쾌락적 개념의 제품군으로 구분하여 효용적 개념의 제품군으로 노트북, 세탁기 그리고 쾌락적 컨셉의 제품군으로 백팩과 운동화를 각각 200개씩 전체적으로 유효 표본을 400개를 선정하여 분석하였다. 분석 결과 효용적 컨셉의 제품은 평균가격, 최고가격, 최저가격, 출시연도, 유통점 수, 리뷰의 수가 가격분산에 유의한 영향을 미치는 것으로 나타났으며, 쾌락적 컨셉의 제품군은 평균가격, 최고가격, 최저가격, 출시연도, 유통점 수, 리뷰 및 평가가 가격 분산과 긍정적인 관계가 있는 것으로 나타났다. 본 연구를 통해서 온라인 가격분산 현상을 확인할 수 있었으며 이와 관련된 변수들도 검증할 수 있었다. 유통점 마케팅 담당자들 관점에서는 가격분산을 유지하기 위해서 역시 이러한 변수들을 고려해서 전략을 수립할 필요가 있을 것으로 보인다. The purpose of this study is to research the effects of the same brand item sold online at different prices. To that end, the exploratory study is focused on four factors that influence price dispersion online is suggested. First, the products are divided into utilitarian products and hedonic products in line with the product concept, and laptops, washing machines are included in the utility concept products. Also the backpacks and sneakers are included in the hedonic concept products. A total of 400 effective objectives were selected and analyzed for each concept products, 200 objectives. The research analysis revealed that the price dispersion is significant relationship with an average price, the highest price, the lowest price, the year of release, the number of retailers, and the number of reviews in case of the utility concept product. On the contrary, in case of hedonic concept products, average price, highest price, lowest price, year of release, retailer, and reviews and ratings is found to be positively related to price dispersion. Based on these results, theoretical and practical implications are discussed about the influence price dispersion on marketing.

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