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대장균에서 무작위 샤인-달가노 서열이 소성장호르몬 유전자 발현에 미치는 영향
나경수,나경수,백형석,이용세 한국생명과학회 2000 생명과학회지 Vol.10 No.4
In order to search for the effects of Shine-Dalgarno (SD) sequence and nucleotide sequence of spacer region (SD-ATG) on bGH expression, oligonucleotides containing random SD sequences and a spacer region were chemically synthesized. The distance between SD region and initiation codon (ATG) was fixed to 9 nucleotides in length. The expression vectors have been constructed using pT7-1 vector containing a T7 promoter. Positive clones were screened with colony hybridization and named pT7A or pT7B plasmid series. The selected clones were confirmed by DNA sequencing and finally, 19 clones having various SD combinations were obtained. When bovine growth hormone was induced by IPTG in E. coli BL21(DE3), all cells harboring these plasmids produced a detectable level of bGH in western blot analysis. However, various SD sequences did not affect on bGH expression, indicating that the sequences of SD and the spacer region did not sufficiently destabilize mRNA secondary structure of bGH gene. Therefore, these results indicate that the disruption of mRNA secondary structure might be a major factor for regulating bGH expression in the translational initiation process.
Osteomas of the craniofacial region
나경수 대한영상치의학회 2011 Imaging Science in Dentistry Vol.41 No.3
Purpose : The purpose of this study was to present the clinical features of a case series of osteomas in the craniofacial region and to compare them with those described in the dental literatures. Materials and Methods : A retrospective study of 18 patients diagnosed with osteomas in the craniofacial region was performed. The age, gender, location, symptoms, and the radiological findings were recorded. Results : There were 13 women and 5 men from 18 years to 69 years of age (mean age, 42±27 years). Fourteen osteomas were found in the mandible (78%), two in frontal sinus, one in sphenoid bone, and one in maxilla. Conclusion : Osteomas are benign tumors composed of mature compact bone or cancellous bone. They are essentially restricted to the craniofacial skeleton and rarely, if ever, are diagnosed in other bones. Purpose : The purpose of this study was to present the clinical features of a case series of osteomas in the craniofacial region and to compare them with those described in the dental literatures. Materials and Methods : A retrospective study of 18 patients diagnosed with osteomas in the craniofacial region was performed. The age, gender, location, symptoms, and the radiological findings were recorded. Results : There were 13 women and 5 men from 18 years to 69 years of age (mean age, 42±27 years). Fourteen osteomas were found in the mandible (78%), two in frontal sinus, one in sphenoid bone, and one in maxilla. Conclusion : Osteomas are benign tumors composed of mature compact bone or cancellous bone. They are essentially restricted to the craniofacial skeleton and rarely, if ever, are diagnosed in other bones.
두부방사선 계측과 Photographic subtraction을 이용한 측모 두부방사선 규격사진의 재현성에 관한 연구
전선두,나경수 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.2
The reproducibility of cephalography in repeated exposures were studied by tracing and photographic subtraction. The materials consisted of 50 pairs of 'same day' radiograph taken under identical conditions. The evaluation included skull, cervical column, hyoid bone, pharynx, tongue, soft tissue profile resulting 43 items in tracing, and 19 items in photographic subtraction. The results obtained from the differences between each pair were as follows: 1. The means and standard deviations by tracing of skull, cervical column, hyoid bone, pharynx, tongue, soft tissue profile were 0.34±0.62㎜, 1.02±1.59㎜, 1.37±1.78㎜, 0.55±1.16㎜, 0.51±1.51㎜, 0.15±0.36㎜ each. 2. The means and standard deviations by photographic subtraction of skull, cervical column, hyoid bone, pharynx, tongue were 0.09±0.35㎜, 0.70±0.95㎜, 1.22±1.33㎜, 0.53±0.86㎜, 0.27±0.41㎜ each.