Changes in the Neuronal Architecture of the Hippocampus in a 6-Hydroxydopamine-Lesioned Rat Model of Parkinson Disease

김보혜,Poornima D. E. Weerasinghe-Mudiyanselage,Mary Jasmin Ang,이정민,강소희,김종춘,김성호,김중선,정채용,신태균,문창종 대한배뇨장애요실금학회 2022 International Neurourology Journal Vol.26 No.S2

Purpose: Parkinson disease (PD) is a progressive neurodegenerative disorder in which dopaminergic (DAergic) systems are destroyed (particularly in the nigrostriatal system), causing both motor and nonmotor symptoms. Hippocampal neuroplasticity is altered in PD animal models, resulting in nonmotor dysfunctions. However, little is known about the precise mechanism underlying the hippocampal dysfunctions in PD. Methods: Striatal 6-hydroxydopamine (6-OHDA) infusions were performed unilaterally in adult Sprague Dawley rats. Both motor and nonmotor symptoms alongside the expression of tyrosine hydroxylase (TH) in the substantia nigra and striatum were confirmed in 6-OHDA-lesioned rats. The neuronal architecture in the hippocampus was analyzed by Golgi staining. Results: During the 7–8 weeks after infusion, the 6-OHDA-lesioned rats exhibited motor and nonmotor dysfunctions (especially anxiety/depression-like behaviors). Rats with unilateral 6-OHDA infusion displayed reduced TH+ immunoreactivity in the ipsilateral nigrostriatal pathway of the brain. Golgi staining revealed that striatal 6-OHDA infusion significantly decreased the dendritic complexity (i.e., number of crossing dendrites, total dendritic length, and branch points) in the ipsilateral hippocampal conus ammonis 1 (CA1) apical/basal and dentate gyrus (DG) subregions. Additionally, the dendritic spine density and morphology were significantly altered in the CA1 apical/basal and DG subregions following striatal 6-OHDA infusion. However, alteration of microglial and astrocytic distributions did not occur in the hippocampus following striatal 6-OHDA infusion. Conclusions: The present study provides anatomical evidence that the structural plasticity in the hippocampus is altered in the late phase following striatal 6-OHDA infusion in rats, possibly as a result of the prolonged suppression of the DAergic system, and independent of neuroinflammation.

백파의 삼종선에 관한 초의의 비판에 대한 고찰

김보혜 한국동서철학회 2018 동서철학연구 Vol.0 No.90

The dispute on the theory of Seon is the dispute over the doctrine of Seon between Baekpa-Geungseon and Choui-Uisun, its point is made up of the dispute on three kinds of Seon and two kinds of Seon, and the dispute has been continued by the two Seon teachers' disciples. While there was 'a dispute on the same and difference of human nature and material nature' in Korean Confucianism, there was 'a dispute on three kinds of Seon and two kinds of Seon'. Although there is a lot of dispute on the 'three kinds of Seon and two kinds of Seon', this thesis will first introduces the contents and the background of its appearance, and examine what is the issue and gist of Choui's criticism of three kinds of Seon. Through this study, I would like to discuss whether Choui's criticism of three kinds of Seon is valid. Baekpa advocated the three kinds of Seon in Seonmunsugyong(禪文手鏡), whose name comes from the division of the levels of Seon into Patriarch Seon(祖師禪), Tathāgata Seon(如來禪), and Principle Seon(義理禪). On the contrary, Choui disputes Baekpa's Seonmunsugyong in his Seonmunsabyeonmaneo(禪門四辨漫語), which has a form that first introduces Baekpa's theory and then criticizes it. It seems that Baekpa's attempt to allocate all Seon analects to one of the three kinds of Seon intentionally goes too far and undermines the original spirit of Seon according to Choui's criticism. However, it can be said that Baekpa's three kinds of Seon is evidently valuable in that his theory presented the standard to judge the depth and shallowness of Seon that is meant to realize the original face beyond language and logic. 선문논쟁(禪門論爭)이란 조선 후기 고승이었던 백파 긍선(白坡亘璇)과 초의 의순(草衣意恂)사이에서 촉발된 선(禪)에 대한 교리논쟁으로 삼종선(三種禪)과 이종선(二種禪)에 대한 논쟁이 그 핵심을 이루며, 이후 무려 100년이 넘는 시간동안 두 선사의 제자들을 통해서 이어져 나갔다. 18-19세기 한국유학에 ‘인물성동이(人物性同異)논쟁’이 있었다면, 불교계에는 ‘삼종선-이종선 논쟁’이 있었던 것이다. ‘삼종선-이종선’에 관해서는 많은 논쟁거리가 있지만, 본 논문에서는 먼저 백파의 삼종선의 내용과 출현 배경에 대하여 소개한 후, 초의가 삼종선을 비판한 쟁점과 요지가 무엇인가를 살펴 볼 것이다. 이를 통해 ‘초의의 삼종선 비판은 과연 타당한 것인가?’하는 문제에 대하여 논하고자 한다. 백파는 『선문수경(禪文手鏡)』을 통하여, 삼종선을 주창하는데, 삼종선이란 선을 그 경지에 따라 ‘조사선(祖師禪), 여래선(如來禪), 의리선(義理禪)’의 세 종류로 나누어 보는 데에서 붙여진 이름이다. 반면, 초의는 『선문사변만어(禪門四辨漫語)』를 통하여 백파의 『선문수경』을 반박하고 있는데, 먼저 백파의 설을 소개한 후 자신의 비판을 가하는 형식을 띄고 있다. 모든 선어록의 말들을 인위적으로 삼종선의 하나에 배대하려한 백파의 시도는 지나친 점이 있으며, 초의의 지적대로 선의 본래정신을 훼손한 면이 있다고 보여 진다. 그러나 언어와 논리를 떠나 본래면목을 곧장 깨닫고자 하는 선의 특성상 그 깊고 얕음을 판단할 수 있는 기준을 제시했다는 점에서 백파의 삼종선은 분명 가치가 있다고 할 것이다.

Structure and electrochemical properties of electrospun carbon fiber composites containing graphene

김보혜,양갑승 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Electrically conducting carbon nanofiber (CNF) composite mats are prepared by electrospinning blended solutions of graphene oxide and polyacrylonitrile (PAN) in N,N-dimethylformamide (DMF), and their electrochemical properties are investigated as electrodes of supercapacitors. A graphene oxide concentration of 5 wt.% in a PAN polymer solution offers a large specific surface area and good conductivity after a carbonization process at 1000℃. The supercapacitor electrode prepared with 5 wt.% graphene shows high specific capacitance, 146.62 F g-1, and high energy density, 14.83–19.44 W h kg-1, at power densities ranging from 400 to 20,000 W kg-1 in a 6 M KOH aqueous solution.

Effects of Intermittent Fasting on the Circulating Levels and Circadian Rhythms of Hormones

김보혜,주예나,김민선,최한경,Qingchun Tong,권오빈 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.4

Intermittent fasting has become an increasingly popular strategy in losing weight and associated reduction in obesity-related medicalcomplications. Overwhelming studies support metabolic improvements from intermittent fasting in blood glucose levels, cardiac andbrain function, and other health benefits, in addition to weight loss. However, concerns have also been raised on side effects including muscle loss, ketosis, and electrolyte imbalance. Of particular concern, the effect of intermittent fasting on hormonal circadianrhythms has received little attention. Given the known importance of circadian hormonal changes to normal physiology, potentialdetrimental effects by dysregulation of hormonal changes deserve careful discussions. In this review, we describe the changes in circadian rhythms of hormones caused by intermittent fasting. We covered major hormones commonly pathophysiologically involvedin clinical endocrinology, including insulin, thyroid hormones, and glucocorticoids. Given that intermittent fasting could alter boththe level and frequency of hormone secretion, decisions on practicing intermittent fasting should take more considerations on potential detrimental consequences versus beneficial effects pertaining to individual health conditions.

연고기제의 침투력 시험

김보혜,조순자,조화옥,이경구,한형주 이화여자대학교 약학회 1966 梨花藥學會誌 Vol.- No.7

The epidermis is wholly covered with fat film and can not allow to penetrate some substances(medicaments) through the skin. Then hydrophillic substances are difficult to penetute through the healthy skin because of epidermis is hypophillic property but when the skin should be wounded by circumstances, secretion squeezes out of the inner tissue and spreads on the surface of the skin to form hydrophillic property. We planned to study for the penetration of the ointment bases with agar-culture instead of wounded skin, because the agar-culture is as similar as the wounded skin in every condition. The Ointment bases used for study are classified as following 4 groups. 1. Oleaginous bases: vaselin, lanolin, lanolin+vaselin. 2. Absorption bases: cold cream. 3. Emulsion bases: vanishing cream hydrophillic ointment. 4. Water soluble bases: carbowax. Consequently, we got such experimental results that watersoluble bases are the best of all in penetration.

연고기제의 침투력 시험

김보혜,조순자,조화옥,이경구,한형주 梨花女子大學校 藥學硏究所 1966 梨花藥學硏究 Vol.- No.7

The epidermis is wholly covered with fat film and can not allow to penetrate some substances(medicaments) through the skin. Then hydrophillic substances are difficult to penetute through the healthy skin because of epidermis is hypophillic property but when the skin should be wounded by circumstances, secretion squeezes out of the inner tissue and spreads on the surface of the skin to form hydrophillic property. We planned to study for the penetration of the ointment bases with agar-culture instead of wounded skin, because the agar-culture is as similar as the wounded skin in every condition. The Ointment bases used for study are classified as following 4 groups. 1. Oleaginous bases: vaselin, lanolin, lanolin+vaselin. 2. Absorption bases: cold cream. 3. Emulsion bases: vanishing cream hydrophillic ointment. 4. Water soluble bases: carbowax. Consequently, we got such experimental results that watersoluble bases are the best of all in penetration.

교시교과목의 효과적 전달을 위한 방안 연구 : 시청각 교구 이용의 필요성과 활용방안을 중심으로

김보혜 건국대학교 1996 常虛思想 Vol.7 No.-

大正期 政官關係의 變化와 第2次 護憲運動의 展開 - 帝都復興計劃의 축소과정을 중심으로

김보혜 서울대학교 동양사학과 2011 서울大 東洋史學科 論集 Vol.35 No.-

치매임상평가척도 박스총점(CDR-SB)에 따른 기억성 경도인지장애와 혈관성 경도인지장애의 MMSE, HDS-R 및MoCA 검사 반응 비교

김보혜,강연욱 대한치매학회 2014 Dementia and Neurocognitive Disorders Vol.13 No.4

Background: O’Bryant et al. (2008) argued that the CDR Sum of Boxes (CDR-SB) can be used to distinguish MCI from very early dementia in patients with CDR-GS of 0.5. They reported that the optimal CDR-SB cut-off score was 2.5. The present study was conducted to examine whether the subgroups classified with CDR-SB scores showed the corresponding group differences on the cognitive tests. Methods: The subjects were 45 amnestic multi-domain MCI (amMCI) and 53 vascular MCI (VaMCI) with CDR-GS of 0.5. Each patient group was classified into “mild (0.5-2.0)” and “severe (2.5-4.0)” subgroups based on the CDR-SB. As the result, 4 groups were formed such as mild amMCI (n=23), severe amMCI (n=22), mild VaMCI (n=29), and severe VaMCI (n=24). The subjects were given the MMSE, HDS-R, and MoCA. The MANCOVA was conducted separately for each test with a Bonferroni correction for multiple comparisons. Results: Severe groups of both amMCI and VaMCI showed significantly lower performances than two mild groups in MMSE, HDS-R, and MoCA. It was found that “serial 100-7” was a good item to discriminate between the mild and severe groups in both amMCI and VaMCI. Items measuring “orientation” or “naming” showed significant subgroup differences in the amMCI, whereas items related to “memory,” “visuospatial construction,” or “frontal/executive function” showed significant subgroup differences in the VaMCI. Conclusions: These results showed that there were significant differences in cognitive functions between the two subgroups with CDR-GS of 0.5. The results about the items discriminating between subgroups in amMCI and VaMCI were consistent with the previous findings about the progression of cognitive impairments in AD and VaD. Therefore, these results support the use of CDR-SB as a guideline for distinguishing MCI from early dementia.

산복사나무 추출물의 궤양성 대장염증 완화 검증

김보혜,이용준,김희준,이미라,조계춘,라문진 강원대학교 농업생명과학연구원 2023 강원 농업생명환경연구 Vol.35 No.4

Prunus Davidiana, known as wild peach, has been used as a medicinal material against various inflammatory diseases since ancient times and belongs to the family Rosaceae. This study aimed to investigate the effect of wild peach extract (WPE) on inflammatory bowel diseases IBD at two different doses (10 and 100 mg/kg). Colitis was induced in mice (C57BL/6) by administering 2.0% Dextran sodium sulfate (DSS) in drinking water for 5 days. WPE treatment significantly improved the disease activity index, addressing symptoms such as body weight loss, bloody stool, stool consistency, and colon shortening. Furthermore, the increased levels of myeloperoxidase and pro-inflammatory cytokines (IL-6 and IL-22) with the development of colitis following DSS administration were significantly decreased by WPE. Histological analysis with Hematoxylin & Eosin staining of the colon tissue suggested that WPE significantly reduced colon damage. Additionally, pro-inflammatory cytokine (IL-8) and reactive oxygen species levels were reduced in HT-29 cells. Based on these results, we suggest that P. davidiana can ameliorate DSS-induced colitis in a mouse model. Furthermore, we anticipate that P. davidiana could serve as an effective therapeutic candidate for the treatment of inflammatory bowel diseases.