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대량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별
이숭덕,김기범,이윤성,최영태,신창호,이정빈 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1
The mitochondrial DNA(mtDNA) is a small extranuclear DNA molecule that has been sequenced in man. the mtDNA has distinct characteristics over nuclear DNA. First, the evolutionary rate of nucleotide substitution appears to be larger compared with the nuclear DNA. Second, mtDNA is maternally inherited, so the determination of the various haplotype is unequivocal and no recombination has to be involved. Third, there is a 1.1 kb long control region, which shows severe polymorphism. These characteristics have been applied for several evolutionary study. Furthermore, they exist in cytoplasm with numerous copy number and their size, 16,569 bp is small relative to nuclear DNA, so they are more resistant to degradation or can be types in samples with only cytoplasm such as hair shaft without hair root. These presented the possibility of mtDNA as a tool in individual identification, especially when the nuclear DNA be unavailable. Actually, polymorphism in control region has been applied to individual identification for the skeletal remain found in Vietnam war. So we decided to apply the polymorphism of mtDNA in control region for the individual identification in case of mass disaster-"Downfall of Sampoong Department". Human remains from total 27 different individuals and 178 control persons were submitted for the individual identification. As the mtDNA comparison could be done through maternal lineage, 12 individual from 9 families from the control group were ruled out, because these families were consisted of father or were seeking for their father. Sequencing for the mtDNA control region was done using ABI automatic sequencer and DyeDeoxy Terminator Cycle Sequencing Kit. For convenience, sequencing reaction was done for two separate control region, region I from 16016-16401 in Anderson sequence, and regionⅡ from 048-388. First, sequences in regionⅡ of all samples and control persons were compared, and 20 samples were ruled out with no identical sequences in control group. Remaining 7 samples showed identical sequences with some of the control persons in three groups, and these identical pairs underwent mtDNA region I study. After region I study all samples except two dropped out in blood relationship. For the confirmation, STR and VNTR study such as MCT118, apoB, YNZ22, vWF, MBP, D21S11, TC-11 were done for remaining the two samples. The result denied the blood relationship between these two samples and any of the control group.
Long-term postresection prognosis of primary neuroendocrine tumors of the liver
Jay Jung,Shin Hwang,Seung-Mo Hong,Ki-Hun Kim,Chul-Soo Ahn,Deok-Bog Moon,Tae-Yong Ha,Gi-Won Song,Yo-Han Park 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.97 No.4
Purpose: Primary hepatic neuroendocrine tumor (PHNET) is a very rare neoplasm, requiring strict exclusion of metastasis from possible extrahepatic primary sites for its diagnosis. Methods: We reviewed our clinical experience of 13 patients with primary hepatic NET who underwent liver resection from January 1997 to December 2015. Results: The mean age of the 13 patients (8 males and 5 females) was 51.1 ± 12.8 years; the most common clinical manifestation was vague, nonspecific abdominal pain (n = 9). Of them, 11 patients underwent preoperative liver biopsy, 7 of which correctly diagnosed as neuroendocrine tumor (NET). Ten patients underwent R0 resection, and 3 underwent R1 resection. Diagnosis of PHNET was confirmed both immunohistochemically and by absence of extrahepatic primary sites. All tumors were single lesions, with a mean size of 9.6 ± 7.6 cm and a median size of 4.3 cm; all showed positive staining for synaptophysin and chromogranin. During a mean follow-up period of 95.1 ± 86.6 months, 7 patients died from tumor recurrence, whereas the other 6 remain alive to date, making the 5-year tumor recurrence rate 56.0% and the 5-year patient survival rate 61.5%. When confined to R0 resection, 5-year recurrence and survival rates were 42.9% and 70.0%, respectively. Univariate analysis showed that Ki-67 proliferative index was the only risk factor for tumor recurrence. Conclusion: PHNET is a very rare tumor with no specific clinical features, and its final diagnosis depends primarily on pathology, immunohistochemistry, and exclusion of metastasis from other sites. Aggressive surgical treatment is highly recommended for PHNET because of acceptably favorable postresection outcomes
한국인에서 Quadruplex PCR을 이용한 8종류 상염색체 STR 유전좌의 다형성 분석
장영길,김기범,신창호,이숭덕,이윤성,이정빈 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1
Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5% polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 085 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65×10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here preyed to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.
남민우,조찬희,정한섭,김홍덕,신기석 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-
본 연구는 증기발생기 건전성 향상기술 개발 과제 일환으로 진행중인 고리 1호기 구 증기발생기 인출 전열관을 대상으로 하는 Round Robin Test의 사전 시험을 위해 수행하였다. 균열결함에 대한 검출 및 크기측정의 기준을 설정하기 위해 SGMP Mock-Up를 제작하였고 신호수집한 Data를 증기발생기 전열관 가동중검사 업무를 수행하고 있는 와전류신호평가자들 4개조가신호평가에 참여하였으며, 그 결과를 요약 정리하였다. 본 연구에서 SGMP Mock-Up Data Crack Sizing의 목적은 고리 1호기 구 증기발생기 전열관을 대상으로 시행될 Round Robin Test 전에 결함 기구별 균열결함의 특성 이해 및 균열결함이 여러 유형의 외부 환경에서 나타나고 있을 때의 균열결함에 대한 정량적인 크기 측정값의 바람직한 모델 적용을 확인하기 위함이다. SGMP Mock-Up에 적용한 와전류검사는 Robbin 및 MRPC 검사를 포함하였다. 본 연구의 주 목적인 MRPC 데이터를 이용한 균열깊이 평가에 2개조는 위상각-깊이 분석법을 적용하여 신호평가를 수행하였고, 2개조는 위상각-깊이 분석법 이외에 진폭-깊이 평가 기법을 적용하여 SGMP Mock-Up에 대한 최적의 기법을 설정하였다.