축구 오버헤드 킥 동작의 운동학적 분석

김의환,이요열,김성섭,권문석,김성호 한국운동역학회 2003 한국운동역학회지 Vol.13 No.1

Kim, E-H · Lee , Y-Y · Kim, S-S· Kwon, M-S · Kim, S-H. The kinematical Analysis of the Overhead Kick on Soccer. Korean Journal of Sport Biomechanics. Vol. 13, No. 1,pp. 155-171. The purpose of this study was to analyze the kinematic variables of over head Kick(OHK) in soccer with three dimensional analysis technique and show the kinematic characteristics of it. The 7 subjects were university football player who have been playing football more than 7 years. The OHK was filmed on 16mm video camera(30frame/sec.) kinematic variables were temporal, postures, and COG(center of gravity). The mean values and the standard deviation for each variables were obtained and used as basic factors for examining characteristics of OHK. the results of this analysis were as follows : Temporal variables : The total time elapsed(TE) of OHK was0.94~1.14sec., the 1st phase was 0.35sec., 2nd phase was 0.46sec., and 3rd phase was 0.22sec.. Posture variables : When subjects performed OHK at the impact event, the ankle and knee angle of kicking foot were more extend than supporting foot. but the hip angle of supporting foot were more extend than kicking foot. Moving distance of the center of mass of the both foot : When subject performed OHK at the impact event, the range of distance on mediolateral direction aspect into right · left shoulder line, anteroposterior direction aspect was 20.9±10.5cm, vertical direction aspect was 92.3±19.9cm. Angular velocity : the faster angular velocity of knee · ankle on the kicking foot grew form jump position to landing position, the faster velocity of ball became. C. O. G. variables : When subject performed OHK at the impact event, upper part of the body was getting lower, lower part of the body was getting higher.

골다공증 환자 혈청이 정상인 조골세포의 성장과 분화에 미치는 영향

김진,이재훈,김경욱,이광원,한상배,김환묵 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.4

Osteoporosis is a condition in which an imbalance appears between bone resorption and formation, with bone resorption exceeding formation. Recent studies have shown that bone formation abnormalities in various forms of osteopenia result mainly from defective recruitment of osteoblastic cells. These abnormalities in osteoblast function and bone formation are associated with alterations in the expression or production of several growth factors, such as TGF-β which modulate the proliferation and activity of bone-forming cells. Bone transplantation is an absoulte requirement in several patholigical conditions. The growth factors, such as TGF-β, PDGF, were the effective in promoting growth of the bone in vitro and in animal models. We have investigated the effects of PDGF and TGF-β on the proliferation and differentiation of the normal human osteoblast in vitro culture. The normal human osteoblast from iliac bone were primarily cultured. The serums obtained from the osteoporotic patients and the normal was used to quantify PDGF and TGF-β from the osteoporotic patients serum and the normal serum. To clarify the effects of the various different the culture conditions such as 1×10 exp(4) cells/㎖, 2.5×10 exp(4) cells/㎖, 5×10 exp(4) cells/㎖, 10×10 exp(4) cells/㎖ (0.2∼2×10 exp(4) cells/well) of the osteoblast at 24 hours, 48 hours, 72 hours under 10% FBS, 10% normal human serum, 10% osteoporotic human serum, 3% normal human PRP, 3% osteoporotic human PRP. The cell proliferation and differentiation was determined by [^3H]-thymidine and SRB assay, and the magnitude of differentiation to osteoblast was confirmed by von Kossa staining and alkaline phosphatase stain and measuring the alkaline phosphatase activity during 48 hours and 72 hours. Statistical differences were evaluated using the scheffe's test. The ANOVA procedure of the SAS system. The obtained results were as follows ; 1. The age distribution of normal human was 36.9±4.4 old, osteoporotic human was 72.5±0.2 old with statistical significant difference(p<0.05). 2. The quantification of TGF-β of the normal human serum was 39658.38±11630.43 pg/㎖, the osteoporotic human serum was 30459.40±1704.92 pg/㎖ with statistical significant difference. The quantification of PDGF of the normal human serum was 3064.13±709.51 pg/㎖, the osteoporotic human serum was 2514.13±140.21 pg/㎖ with no statistical significant difference(p<0.05). 3. The DNA synthesis and protein assay of human osteoblast at 24 hours, 48 hours, 72 hours was similar increased to 10% normal human seurm, 10% osteoporotic human serum. There was no statistical significant difference between the normal human and the osteoporotic patients in 3% normal human PRP and 3% osteoporotic human PRP. 4. The optimal cell concentration was 5×10 exp(4) cells/㎖ among 1×10 exp(4) cells/㎖, 2.5×10 exp(4) cells/㎖, 5×10 exp(4) cells/㎖, and 10×10 exp(4) cells/㎖. The DNA synthesis was decreased after 72 hours in the normal human serum and PRP, the osteoportic serum and PRP. 5. The alkaline phosphatase activity was as the same result 10% FBS, 10% osteoportic serum and 10% normal human serum at 48 hours with no statistical significant, but the alkaline phosphatase activity was increased in 10% osteoportic human serum and 10% normal human serum except 10% FBS at 72 hours. From above result, the amount of TGF-β of the normal human growth factor was higher than the osteoporotic patients, but the growth factors of the osteoportic patients were enough the proliferation and differentiation of normal human osteoblasts such like the same effects of normal human growth factors.

실리콘 겔에 활성화된 복강 대식세포의 interleukin-6 및 tumor necrosis factor-α에 의한 섬유모세포 중식 자극

김환묵,한상배,이백권,이종원,한기택,천지훈 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.5

Silicone gel breast implants may induce local(fibrous capsular contracture) or systemic(rheumatoid arthritis, systemic sclerosis, etc) complications. The exact mechanism of fibrous capsular contracture has not been fully understood. In the present study, we tried to find out the effect of silicone gel on the fibroblast proliferation which has been known as a major contributing factor in fibrous capsular contracture formation. In vitro, activated macrophages are known to secrete monokines which affect fibroblast proliferation and collagen synthesis. And tumour necrosis factor-α(TNF-α) and interleukin-6(IL-6), which were released by macrophages, were reported as potent stimulator of fibroblast proliferation. The goal of this study is to investigate the role of macrophages and tumour necrosis factor-αor interleukin-6 in the interaction of fibroblasts and silicone gel. We designed four groups, two experimental and two control, using Institute for Cancer Research(ICR) mouse peritioneal macrophage and silicone gel. For the preparation of the conditioned medium of macrophages, peritoneal macrophages were prepared and cultured for 24 hours on the silicone gel-coated and naked (not coated) surface [silicone gel-macrophage conditioned medium(SCM; experimental group) and normal polystyrene-macrophage conditioned medium(NCM; control group) respectively]. To correct the effect of 10% fetal bovine serum which was included in Rapid Prototyping and Manufacturing Institute (RPMI) 1640 medium and draw the effect only by macrophages, the RPMI 1640 medium with 10% fetal bovine serum was cultured by the same method on the silicone gel-coated and naked surface (silicone gel-macrophage free conditioned medium; SFM and normal polystyrene-macrophage free conditioned medium; NFM respectively). Each conditioned medium was added onto NIH 3T3 fibroblasts culture at a final 25% concentration of total culture medium and followed by the cultivation for 24 hours. For antibody neutralizing experiments, each conditioned medium was preincubated with polyclonal rabbit anti-mouse TNF-α antibody or polyclonal rat anti-mouse IL-6 antibody for 1 hour and then, conditioned medium with antibody was added to the culture medium of NIH 3T3 fibroblasts by the same method. After 24 hours cultivation, total number of viable fibroblast(cell growth), DNA synthesis and collagen synthesis of fibroblasts with each medium were measured by sulforhodamine B(SRB) assay, 3H-thymidine and 3H-proline incorporation respectively. The results were as follows: 1. In the experiment about the effect of the conditioned medium on the fibroblast activity, the experimental group(SCM), compared with the control group(NCM), showed a significant increase of the cell growth (p<0.01), a significant decrease of DNA synthesis(p<0.001), but no significant difference in the collagen synthesis. 2. In the experiment about the effect of polyclonal rabbit anti-mouse TNF-α antibody on the fibroblast activity, after the addition of antibody the experimental group, compared with the control group, showed a significant decrease of the cell growth(p<0.001), a significant increase of DNA synthesis(p<0.01), but no significant difference in the collagen syn thesis. 3. In the experiment about the effect of polyclonal rat anti-mouse IL-6 antibody on the fibroblast activity, after the addition of antibody the experimental group, compared with the control group, showed a significant decrease of the cell growth(p<0.001), a significant increase of DNA synthesis(p<0.0001), but no significant difference in the collagen synthesis. In conclusion, culture supernatants (conditioned medium) of peritoneal macrophages, activated by silicone gel, stimulate the NIH 3T3 fibroblast proliferation. TNF-α and IL-6, products of macrophage, are involved in the stimulation of NIH 3T3 fibroblast proliferation in an in vitro condition.

국내산 및 제초제 내성 콩(HS2906)의 일반성분, 무기질 및 지방산 조성

양윤형,이정희,김형진,윤원기,김환묵,김미리 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

Proximate analysis, mineral and fatty acid composition of three conventional domestic soybean cultivars and two imported ones including glyphosate-tolerant HS2906 were evaluated by AOAC method, ICP-AES and gas chromatography. There were several differences in the proximate analysis among three conventional domestic soybean cultivars ; higher crude fat in the cultivar Hwanggumkong, higher crude protein in Pungsankong, and higher carbohydrate and crude ash in Duyukong. The ranges of contents of proximate components of domestic cultivars were similar to the data previously reported. There were no significant differences in proximate analysis between conventional soybean WS82 and glyphosate-tolerant HS2906 ; 23.55~23.90% of crudefat, 34.22~35.55% of crude protein, 6.25~6.45% of crude ash, and 25.35~26.47% of carbohydrate. The mineral and fatty acid compositions of HS2906 were similar to those of conventional soybeans previously reported.

Inhibition of Human Ovarian Tumor Growth by Cytokine-induced Killer Cells

Kim, Hwan-Mook,Kang, Jong-Soon,Lim, Jae-Seung,Park, Song-Kyu,Lee, Ki-Ho,Yoon, Yeo-Dae,Lee, Chang-Woo,Lee, Ki-Hoon,Han, Gyoon-Hee,Yang, Kyu-Hwan,Kim, Yeon-Jin,Kim, Young-Soo,Han, Sang-Bae 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.11

Despite the recent improvement in the treatment of ovarian cancer, this disease is still leading cause of cancer death in women. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human ovarian cancer was evaluated in vitro and in vivo. Although $CD3^+CD56^+$ cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1,000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 45% of SK-OV-3 human ovarian cancer cells, which was determined by the $^{51}Cr-release$ assay. In addition, CIK cells at a dose of 23 million cells per mouse inhibited 73% of SK-OV-3 tumor growth in nude mouse xenograft assay. This study suggests that CIK cells may be used as an adoptive immunotherapy for patients with ovarian cancer.

Toxicokinetics of CJ-11555: Gender Difference and M inim um Accum ulation

Kim Young Hoon,Kim II Hwan,Noh Hyun Jung,Choi Jae Mook,Kim Deog Yeor,Park Jie Eun,Lee Sung Hak,Kim Taekrho,Kim Jin Wan 대한약학회 2003 大韓藥學會 總會 및 學術大會 Vol.2003 No.2

Antidiabetic Activity of Angelan Isolated from Angelica gigas Nakai

Kim, Hwan-Mook,Kang, Jong-Soon,Park, Song-Kyu,Lee, Ki-Ho,Kim, Jee-Youn,Kim, Yeon-Jin,Hong, Jin-Tae,Kim, Young-Soo,Han, Sang-Bae 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.11

Angelan isolated from Angelica gigas Nakai inhibits tumor growth and metastasis by enhancing immune functions of macrophages, dendritic cells, and B cells. Here, we report that angelan can inhibit autoimmunity in non-obese diabetic (NOD) mice. Although 80% of the NOD mice had developed diabetes by 24 weeks of age, none of the angelan-treated NOD mice developed diabetes. The mean glucose levels were 118 mg/dl in angelan-treated mice and 506 mg/dl in control NOD mice. Histological examination of the pancreatic islets revealed that most of the islets isolated from angelan-treated mice were less infiltrated with lymphocytes compared with those of control mice. Spleen cells from diabetic NOD mice could adaptively transfer diabetes into NOD.scid mice, but those from angelan-treated NOD mice did not, suggesting that angelan caused the spleen cells to lose the ability to destroy $\beta$ cells. However, angelan did not affect cytokine production of spleen cells. These results suggest that angelan has dual immunomodulatory functions, i.e., immunostimulation in tumor-bearing mice and immunosuppression in autoimmune diabetic mice.

Persistence of Genetically Modified Potatoes in the Field

Kim, Chang-Gi,Kim, Do-Young,Moon, Ye-Seul,Kim, Hyo-Jeong,Kim, Dae-In,Chun, Young-Jin,Park, Kee-Woong,Jeong, Soon-Chun,Kim, Soo-Young,Kim, Hwan-Mook 한국식물학회 2010 Journal of Plant Biology Vol.53 No.6

Volunteers from genetically modified (GM) potatoes may pose an environmental problemif allowed to grow in the field after the annual crop is harvested. We tested whether they are more likely to produce volunteers than non-GM potatoes. Specifically, we compared the number of volunteers, number of tubers per plant, tuber size, and their vertical distribution in the soil. More volunteer plants came from non-GM potatoes than from GM potatoes, but the number and size of tubers were similar between the two. Vertical distribution of the tubers differed significantly, with most non-GM tubers being found in shallower soil (<2 cm deep). Our results suggest that spontaneous GM volunteers may emerge and produce tubers to a degree similar to that of the non-GM plants. No viable volunteers emerged from GM tubers in the next growing season, probably deterred by winter frost and a period of low soil temperatures (below $-2^{\circ}C$) at our study site. However, in regions with warmer climates, such GM volunteers may survive Winter and produce more plants the following year.

Role of Polysaccharides Isolated from Angelica Gigas Nakai in Dendritic Cell Maturation

Jee Youn Kim, Jong Soon Kang, HWAN Mook KIM, Song-Kyu Park, Kiho Lee, Yeon Jin Kim, Jin Tae Hong, Yo 충북대학교 약품자원개발연구소 2008 약학논문집 Vol.23 No.-

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CJ-11555의 Sprague-Dawely 랫드를 이용한 단회 및 4주 반복경구투여 독성시험

김일환(Il-Hwan Kim),이성학(Sung-Hak Lee),최재묵(Jae-Mook Choi),박지은(Jie-Eun Park),김덕열(Deog-Yeor Kim),노현정(Hyun-Jung Noh),김택로(Taek-Rho Kim),이상호(Sang-Ho Lee),김영훈(Young-Hoon Kim),김진완(Jin-Wan Kim),장준환(Jun-Hwan Chang 한국독성학회 2004 Toxicological Research Vol.20 No.1

This study was to investigate single and repeated-dose toxicities of CJ-11555, an anticirrhotic agent, in Sprague-Dawley (SD) rats. In single-dose oral toxicity study, the test article were administered once by gavage to males and females at dose levels of 0 and 2,000 mg/kg. No dead animals and abnormal necropsy findings were found in control and CJ-11555 treated group. Therefore, the approximate lethal dose of CJ-11555 was considered to be higher than 2,000 mg/kg in rats. In the 4-week repeated oral toxicity study, the test article was administered once daily by gavage to<br/> male and female rats at dose levels of 0, 10, 50 and 200 mg/kg/day for 4-weeks. In clinical signs, yellow-colored urine and yellow hair coat were observed in the 50 and 200 mg/kg male and female<br/> groups. In hematology, erythrocyte count and hemoglobin were significantly decreased in the 200mg/kg male and female groups. In serum biochemistry, total cholesterol was significantly increased and aspartate aminotransferase (AST) was significantly decreased in the 50 or 200 mg/kg male and female groups. In histopathological examinations, centrilobular hepatocellular hypertrophy in the liver, congestion and pigmentation in the spleen, hyaline droplets in the kidney were observed in the 50 and 200 mg/kg male and female groups. In toxicokinetic study, CJ-11555 was dose-dependent in systemic exposure and showed better absorption in female with minimum accumulation after multidosing. Based on these results, it was concluded that the 4-week repeated oral dose of CJ-11555 resulted in the suppression of AST activity and centrilobular hepatocellular hypertrophy in both sexes at a dose level of 50 or 200 mg/kg/day. The target organ was estimated to be liver, spleen and male's kidney. The no-observed-adverse-effect level (NOAEL) for CJ-11555 in rats following gavage for at least 4-week is 10 mg/kg/day.