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( Jeong Hwan Youk ),( Hyunk Yung Park ),( Young Il Koh ),( In Ho Kim ),( Seon Yang Park ),( Sung Soo Yoon ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: POEMS (polyneuropathy, organomegaly, endocrinopathy, monoclonality and skin changes) is a very rare monoclonal disorder. Except for a few case reports, clinical and laboratory characteristics of Korean patients with POEMS syndrome have not been uncovered yet. Methods: We reviewed the medical records of patients with POEMS syndrome in Seoul National University Hospital from January 2000 to June 2014. Patients who met the mandatory criteria of POEMS syndrome ((1) the evidence of monoclonality; (2) the evidence of polyneuropathy that is not explained by other causes ) were included. Comparison with historical control of multiple myeloma was performed. (Mayo Clin Proc. 2003 Jan;78(1):21-33. ) Results: A total of twenty-four patients (Male/Female = 15/9, median age = 52. 6 years old) fulfi lled the mandatory criteria. Osteosclerotic bone lesions and castleman`s disease were found in 45% and 12. 5% of patients, respectively. All of light chains in patients with systematic disease were λ. (IgA/IgG=12/9, light-chain disease=2) Only one patient had a plasmacytoma. The median overall survival (OS) was 64. 4 months. Six patients had autologous stem cell transplantation and they were all alive. Not a single clinical or laboratory factors predicted OS in these population. Interestingly, hemoglobin level(14. 3 g/dL vs 12. 4 g/dL; p=0. 012) and quantity of serum M-protein(0. 95 g/dL vs 0. 17 g/dL; p=0. 015) were significantly different between IgG and IgA subgroups. Compared to multiple myeloma, age at diagnosis was younger and hemoglobin and platelet level tended to be higher in POEMS syndrome. The median OS was longer than that of multiple myeloma. Conclusions: Patients with POEMS syndrome have unique features compared to those of multiple myeloma. In addition, it has been fi rst revealed that the laboratory fi ndings between immunoglobulin subgroups in POEMS syndrome are different.
Jeong, Soon-Chun,Pack, In Soon,Cho, Eun-Young,Youk, Eun Soo,Park, Sangkyu,Yoon, Won Kee,Kim, Chang-Gi,Choi, Yang Do,Kim, Ju-Kon,Kim, Hwan Mook Elsevier 2007 FOOD CONTROL Vol.18 No.11
<P><B>Abstract</B></P><P>The remarkable amount of public and scientific debate with regard to the safety of genetically modified (GM) crops has resulted in the implementation of mandatory risk assessments of newly developed GM crops, as well as mandatory labeling of GM crops and foods following their commercial release. Among the many currently available GM rice lines, transgenic rice lines that overexpress a trehalose-6-phosphate synthase/phosphatase (<I>TPSP</I>) fusion gene have attracted particular interest from researchers, by virtue of their superior tolerance to a variety of abiotic stresses. The primary objective of this study was to determine the copy numbers of the transgene element, and to identify the genomic sequences flanking the integration site of the transgene element in a selected <I>TPSP</I> transgenic rice line, in order to develop new event-specific detection techniques. The genomic sequences flanking the integration site of the transgene element were identified via an inverse PCR protocol. It was determined that a single copy of the <I>TPSP</I> fusion had been integrated into the transgenic line. An effort to find a rice sequence usable as an internal positive control for the screening of the transgenic rice resulted in the identification of an <I>RBE4</I> gene sequence, which appears as one copy within the rice genome, and is rice-specific. The characterization of the genomic sequences flanking the transgene, as well as the availability of the internal positive control sequence, enabled the design of a qualitative PCR technique and a quantitative TaqMan-based detection method for this transgenic rice line. The results of this study may prove useful with regard to the large-scale screening of this transgenic rice line in the processes of risk assessment and commercialization, as well as in the development of novel methods for the detection of other transgenic rice lines.</P>
Genetic Analysis and Event-Specific Detection of a CGMMV-CP Transgenic Watermelon Rootstock Line
Eun Soo Youk,In Soon Pack,Yu-Jin Kim,Won Kee Yoon,Chang-Gi Kim,Stephen B. Ryu,Chee Hark Han,Soon-Chun Jeong,Hwan Mook Kim 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Transgenic plants that over express virus coat protein genes have attracted particular interest from researchers, by virtue of their tolerance to virus infection. The transgenic watermelon rootstock analyzed in this study was established by introducing CGMMV coat protein (cp) under the control of CaMV 35S promoter and NOS terminator (Park et al., (2005) Plant Cell Rep. 24: 350-6). The primary objective of this study was to determine the copy number and integration site of the transgene element, in order to develop detection techniques required for monitoring of the transgenic watermelon rootstock. The Southern blot analysis indicated that a single copy of CGMMV-cp gene was inserted into the genome of transgenic watermelon rootstock. We also identified the genomic sequences flanking the integration site of the transgene by inverse PCR analysis. In an effort to find a sequence usable as an internal positive control for the screening of the watermelon and watermelon rootstock, we found that the Sat and DIP-1 genes appears as one copy within their genomes and is watermelon rootstock- and watermelon-specific. The information of the integrated site and the internal positive control sequence was used to establish a new event-specific PCR-based detection method. In addition, mRNA and protein expression level of the transgene in the transgenic watermelon rootstock and grafted watermelon were investigated. The expression of both mRNA and protein of CGMMV-CP was not detected in the transgenic watermelon rootstocks and watermelons, suggesting that the movement of transgene products from transgenic rootstock to watermelon does not occur at our detection level.
Synthesis of High Molecular Weight 3-Arm Star PMMA by ARGET ATRP
Jeon, Hyun-Jeong,Youk, Ji-Ho,Ahn, Sung-Hee,Choi, Jin-Hwan,Cho, Kwang-Soo The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.4
High molecular weight(MW), 3-arm star poly(methyl methacrylate)(PMMA) with a narrow MW distribution($M_n$=570,000 g/mol, PDI=1.36) was successfully synthesized by activators regenerated by electron transfer(ARGET) atom transfer radical polymerization(ATRP). The polymerization was carried out with a trifunctional initiator/$CuBr_2$/N,N,N',N",N"-pentamethyldiethy lenetriamine(PMDETA) initiator/catalyst system in the presence of a tin(II) 2-ethylhexanoate [$Sn(EH)_2$] reducing agent at $90^{\circ}C$. The concentration of the copper catalyst was as low as 30 ppm, and a high initiation efficiency of the initiating sites was obtained. The chain-end functionality of the high MW, 3-arm star PMMA was confirmed by a chain extension experiment with styrene via ARGET ATRP, using the same catalyst system.