Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Reliability Considerations of Hybrid Power Systems for Critical Facilities

Choi, Se Jin · Ham, Young Hwan · Kim, Myung Chin 충북대학교 컴퓨터정보통신연구소 2008 컴퓨터정보통신연구 Vol.27 No.2

본문참고

발프로산나트륨과 페니토인과의 약물상호작용

최준식,유재신,박용채,이진환 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

This study was attempted to investigate the pharmacokinetic interaction between sodium valproate (4. 8. 16 ㎎/㎏. i.v.) and phenytoin (4 ㎎/㎏. i.v) in rabbits. The plasma concentration and area under the curve (AUC) of phenytoin were increased significantly (p<0.05. p<0.01) when coadministered with sodium valproate (4. 8. 16 ㎎/㎏) in rabbits. The volume of distribution and total body clearance of phenytoin were decreased significantly (p<0.05. p<0.01) when coadministered with sodium valproate (8. 16 ㎎/㎏) in rabbit. From the results of this experiment. it is desirable that dosage regimen of phenytoin should be adjusted and therapeutic drug monitoring should be performed for reduction of side or toxic effect when phenytoin will be coadministered with sodium valproate in clinical use.

天然 및 養殖産 뱀장어의 蛋白質 및 아미노酸 組成比較

崔鎭浩,林采喚,崔映準,卞大錫,金昌睦,吳成基 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

우리나라 전역에 걸쳐서 分布하고 있는 뱀장어의 營養學的 基礎資料를 提示하기 위하여 天然産과 養殖産으로 分類하여 蛋白質의 組成, 蛋白質의 아미노산酸 組成을 比較, 分析하였다. 그리고 天然 및 養殖産 뱀장어 肉蛋白質의 組成을 엄밀히 檢討하기 위해 肉蛋白質中 比較的 많은 量을 차지하는 筋形質蛋白質과 筋原纖維蛋白質을 SDS-PAG電氣泳動 分析하여 subunit의 差를 比較하였다. 또한 사료蛋白質의 아미노酸 組成을 分析함으로서 肉蛋白質의 아미노酸 組成에 미치는 영향도 아울러 比較, 檢討하였다. 天然 및 養殖産 뱀장어의 粗蛋白質 合量은 各各 14.3%와 15.4%이었으며, 粗脂肪은 25.0%와 29.7%로서 養殖産 뱀장어의 脂肪 含量이 天然産에 比하여 높은 편이있다. 肉蛋白質을 構成하는 蛋白質 組成은 天然産의 경우 筋形質蛋白質이 30.78%, 筋原纖維蛋白質이 59.02%, 細胞內殘渣蛋白質이 9.73%, 基質蛋白質이 2.47%이었으며, 養殖産은 筋形質蛋白質이 31.81%, 筋原纖維蛋白質이 58.37%, 細胞內殘渣蛋白質이 8.16%, 基質蛋白質이 1.80%를 차지하여 天然産과 養殖産뱀장어의 肉蛋白質 組成은 비교적 유사함을 알 수 있었다. 筋形質蛋白質과 筋原纖維蛋白質 分劃의 一部에 대하여 SDS-PAG電氣泳動 分析을 실시한 結果, 天然産 뱀장어의 筋形質蛋白質은 16개의 subunit로 構成되어 있었고, 養殖産은 分子量이 36,500, 46,000, 58,500, 75,000, 170,000, 235,000에 해당하는 4개의 subunit가 더 많은 22개의 subunit로 構成되어 있었다. 한편 筋原纖維蛋白質은 天然産이 14개의 subunit이었으며, 養殖産은 分子量 45,000에 해당하는 未知의 subunit가 하나 더 많은 15개로 이루어져 있었다. 肉蛋白質의 蛾眉노산 組成은 天然産이 養殖産에 比하여 glycine含量이 다소 높은 것을 제외하고는 대체로 비슷하였으며, lysine, aspartic acid, glutamic acid, glycine이 전체아미노酸의 45% 가량을 점하고 있었다. 사료蛋白質의 아미노酸 組成은 뱀장어의 필수아미노산 要求量을 만족시킬 만큼 양호한 組成을 보여주었으며, aspartic acid와 glutamic acid를 제외하고는 魚肉의 축적단백질로서 魚肉蛋白質의 아미노 組成과 비례관계를 갖는 것으로 판단되었다. The muscles of wild and cultured eel, Anguilla japonica, were analyzed for the protein composition and amino acid profile. The differences of the subunit distribution for the sarcoplasmic and myofibrillar proteins were discussed with sodium dodecylsulfate(SDS) polyacryamide gel electrophoresis. The muscle protein in wild eel was composed 30.78% of sarcoplasmic, 59.02% of myofibrillar, 9.73% of residual intracellular and 2.47% of stroma fraction. That in cultured eel was composed of 31. 81%, 58.37%, 8.16% and 1.80%, respectively. The sarcoplasmic and myofibrillar proteins were composed of 16 and 14 subunits in wild eel, and 22 and 15 subunts in cultured eel. The sarcoplasmic protein between wild and cultured muscles showed a similar trend in the subunits, except a few subunits such as 36,500, 46,000, 58,500, 75,000, 170,000 and 235,000 daltons in cultured eel. Only the existence of 45,000 dalton subunit was the difference between wild and cultured eel in myofibrillar protein. The distribution patterns of total amino acid in muscles of wild and cultured eel were found to be very similar trend, although glycine content in wild eel was slightly higher than that in cultured one.

Cortisone이 흰쥐의 口腔粘膜 多糖類에 미치는 影響에 關한 組織化學的 硏究

崔鎭煥 醫藥係社 1965 綜合 醫學 Vol.10 No.5

세계화ㆍ지식기반사회에 대한 중등학교 사회과 교사들의 경제의식 및 의견 분석

최병모,김진영,조병철,안상환 한국경제교육학회 2004 경제교육연구 Vol.11 No.1

산화툴륨의 결함구조와 전기전도성에 대한 연구

최재시,정원양,김진호,이창환 연세대학교 자연과학연구소 1985 學術論文集 Vol.14 No.-

산화툴륨의 전기전도도를 400~1100℃의 온도영역과 10^-6~10^-1atm의 산소분압범위에서 측정하였다. 일정한 산소분압에서 전기전도도의 대수값을 절대온도의 역수에 대하여 도시한결과 직선관계를 얻었으며 inflection point가 나타났다. 활성화에너지는 고온영역에서 34.5kcal/mole, 저온영역에서 19.3kcal/mole이었다. 전기전도도의 산소분압의존성은 직선관계를 나타내었으며 600~1100℃의 온도영역에서 σ∝Po_2^1/5.3, 400~600℃의 온도영역에서σ∝Po_2^1/6.3이었다. 고온영역에서의 주결함은 V_??이며, 저온영역에서는 O_?? 임이 밝혀졌다. 전도도의 온도 및 산소분압의존성을 해석하였으며, 전도메카니즘을 제시하였다. The electrical conductivity of thulium sesquioxide has been measured from 400 to 1100℃ under oxygen partial pressure (Po_2) of 10^-6 to 10^-1 atm. Plots of log conductivity vs. 1/T at constant Po_2 are found to be linear with inflection point and the activation energies obtained from the slopes are 34.5kcal/mole in the higher temperature region and 19.3kcal/mole in the lower temperature region. The Po_2 dependences of the electrical conductivity are found to be linear and closely approximated by σ∝Po_2^1/5.3 in the temperature range of 600-1100℃ and σ∝Po_2^1/6.3 in the range of 400-600℃. The dominant defect is V_?? in the higher temperature region and O_?? in the lower temperature region. The interpretations of conductivity dependences on temperature and Po_2 are presented and conduction mechanisms are suggested to illustrate the data.

부적절한 예방화학요법 후 지연 발현된 Plasmodium vivax 말라리아 1 예

최수철,장상필,김상화,서환조,서진태,김구엽 대한내과학회 1998 대한내과학회지 Vol.54 No.3

Malaria is the world's most important parasitic infection. Although it has been erw5cated from temperate zones including Korea, increasing numbers of travellers visit tropical malarious countries and imported malaria becomes important medical problem in the developed countries. In Korea with increasing travellers to malaria-endemic area, the incidence of imported malaria shows rising tendency same as the developed countries. It becomes important to personal general personal protective measures and chemoprophylaxis to trevellers, when employed in appropriate manner, that can be highly effective in preventing malaria. We recently experienced a case of in Plasmodium vivax malaria with delayed manifestations due to inadequate chemoprophylaxis. A 53-year-old woman with history of trevel to East Africa 4 months ago and chief complaint of fever was diagnosed as tertian malaria. She had irregularly taken prophylatic antimalarial during travel and had not taken it after return but should have taken it at least 4 weeks after return.

Homocysteine과 대사증후군의 관계

최진욱,김선현,염창환,정규철 관동대학교 의과학연구소 2006 關東醫大學術誌 Vol.10 No.1

壓挫시킨 家兎 軟骨의 生存

최동원,송중원,한기환,강진성,정재홍 大韓成形外科學會 1990 Archives of Plastic Surgery Vol.17 No.2

The deviated nose regardless whether it is due to traumatic or congenital origin results in troublesome symptoms coming from airway obstruction. Numerous methods for straightening the severely deviated septum have been described by many authors but none is wholly satisfactory. A new method that the crooked septal cartilage is being flattened by crushing and reimplanting into the septal pocket is introduced. The authors are desirous of knowing about the fate of the crushed cartilage graft. Forty rabbits weighing 2 to 3 kg were divided into 2 groups, septal group and conchal group. The cartilages were obtained from the nasal septum and ear; these cartilages were placed under the paraspinal subcutaneous tissue. Prior to paraspinal iplantation the obtained cartilage was halved; one piece of cartilage being crushed by Cottle's cartilage crusher before its placement into the recipient site and the other was placed without crushing. The cartilage specimens were removed at 6 and 12 weeks postoperatively and these were examined by the naked eyes and light microscope. The results were as follows: 1. The fissures and cleavages of cartilage developed during crushing process are restored to normal by 6 weeks postoperatively. 2. All of the ujncrushed cartilages remained viable in the subcutaneous pockets. The crushed cartilages not only appeared viable but also induced a new cartilage formation beneath the perihondrium at 12 weeks postoperatively and the thickness being increased compaired to the cartilage before crushing. 3. Crushed cartilage remained flat at 12 weeks postoperatively. 4. The degree of strength of the crushed cartilage is increased due to new cartilage formation and fibrosis of perichondrium at 12 weeks postoperatively. These findings support the clinical impression that the crushed autogenous cartilage is viable and can be reshaped according to the contour of the recipient site.