C -11 및 F - 18 표지 콜린의 합성과 체내동태에 관한 연구

전권수,유국현,김상욱,임상무,홍성운,서용섭,양승대,안순혁,허민구 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.3

Objectives: Recently, [methyl-(11)^C]-(β-Hydroxyethyl)trimethylammonium ([(11)^C]choline) has been discovered to be a very effective tracer in imaging various human tumors using positron emission tomography. Because of the short half-life of C-11, it is very difficult to use in a routine imaging procedure and needs a frequent synthesis of [(11)^]choline. This can be supplemented by the substitution of [(11)^Ccholine with [methyS-18]fluorocholine. Here, we would like to report cell uptake and biodistribution of [(11)^Ccholine and [(18)^F]fluorocholine as a basic study. Methods [(11)^C]Choline was prepared by the treatment of [(11)^C]CHzI with N,N-dimethylaminoethanol and [18F]fluorocholine was synthesized from reaction of CHzBr[18F]F with N,N-dimethylaminoethanol. The radiochemical purity was checked by high performance liquid chromatography (HPLC). The biodistribution of [(11)^C]choline and [(18)^F]fluorocholine was determined in balb/c mouse at 5 min, 20 min, 40 min and 80 min. The cell uptake wa measured using glioma (9L) and colon adeocarcinoma (SW620). Results: The radiochemical purity was more than 98% after purification. In the liver, uptake did not change over time the uptake was 20/ID/g for [C]choline and 13%ID/g for [(18)^F]fluorocholine. In the kidney, radioactivity decreased over tirne the uptake was 15%1D/g for [(11)^Ccholine and 20%ID/g for [(18)^F]fluorocholine, 80 min post-injection. The cell uptake of [(11)^Ccholine was 4.93% for glioma (9L) and 18.69F for colon adenocarcinoma (SW620). For [(18)^F]fluorocholine, 1.77% for glioma (9L) and 2.77% for colon adenocarcinoma (SW620). Conclusion: [(11)^CCholine and [(18)^F]fluorocholine showed a different cell uptake tendency, depending on cancer cell line. (Korean J Nucl Med 200135:185-191)

Conditioned Media of ASC-17D Sertoli Cells Induce G1-Growth Arrest of DU145 Human Prostate Cancer Cells

Min, Bon Hong,Kang, Sang Wook,Lee, Kwang Ho,Choi, Sang Hyun,Kang, Hyeog,Chun, Boe Gwun The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.5

We studied the effects of ASC-17D rat Sertoli cell-conditioned media (rSCCM) on the proliferation of the DU145 prostate cancer cells. rSCCM was prepared from ASC-17D cells cultured in DMEM/F-12 serum-free media at a nonpermissive temperature of 40℃, which is the condition for the high expression of clusterin. We found that rSCCM could inhibit the proliferation of DU145 cells by arresting the cell cycle in the G1 phase in a dose-dependent manner. This growth arresting activity was abolished by boiling rSCCM for 5 min. The G1 growth-inhibiting activity of rSCCM was also detected in other prostate-originated cancer cells examined (ie., LNCaP and PC-3) but not in other cells (ASC-17D, HepG2, SK-N-SH, and NIH3T3). Western blot analysis of partially purified growth inhibiting fractions with the clusterin antibody showed that the cytostatic factor in rSCCM was not clusterin. This cytostatic factor was semipurified by DEAE-Sepharose, ammonium sulfate precipitation, and Phenyl-Sepharose column chromatography, and was estimated to have a molecular weight of 88 kDa by Sephacryl S-300 gel filtration.

反芻動物의 肝蛭類 및 胃腸內線蟲類에 對한 Nitroxynil-Levamisole 複合注射劑 詩製品의 驅蟲效果評價試驗

田桂植,閔弘基,張斗煥 梨花女子大學校 醫科大學 醫科學硏究所 1990 EMJ (Ewha medical journal) Vol.13 No.3

The present study was carried out to evaluate the efficacy of the experimentally prepared injection of nitroxynil-levamisole against liver fluke and gastrointestinal roundworms in goats and cattle. For the purpose, the dose confirmation trial, the dose reduction trial and clinical field trial were conducted according to "the guidelines for evaluating the efficacy of anthelmintics in ruminants" approved by the General Meeting of the 9th International Conference of the World Association for the Advancement of Veterin ary Parasitology" in 1981. The results obtained are summarized as follows ; 1) A single dose of 10mg/kg of nitroxynil injection alone against the liver fluke and that of 5.0mg/kg of levamisole injection alone against gastrointestinal roundworms in goats were highly effective in the cure rate and the egg reduction rate, respectively. 2) A single dose of the experimentally prepared compound injection at the dose levels of nitroxynil 10.0mg-12.0mg/kg and levamisole 5.0mg-6.0mg/kg was highly effective in the egg reduction rates of both liver fluke(98.4%-100%) and gastrointestinal roundworms(97.3%-98.4%) without any side reaction in goats. 3) A single does of the experimentally prepared compound injection at the reduced dose level of nitroxynil 7.5mg/kg and levamisole 5.0mg/kg was highly effective in the egg reduction rates of both liver fluke and gastrointestinal roundworms in goats. 4) In the clinical field trial, a single dose of the experimentally prepared compound injection at the reduced dose level of nitroxynil 7.5mg/kg and levamisole 5.0mg/kg showed also very high efficacy in the cure rates and the egg reduction rates of both liver fluke and gastrointestinal roundworms in Korean cattle, dairy cow and beef cattle. However, it was not effective against Ostertagia sp. among gastrointestinal roundworms. No side reactions were noted.

韓國에 있어서의 人體感染 東洋眼蟲에 對한 形態學的 硏究

閔弘基,田桂植 梨花女子大學校 醫科大學 醫科學硏究所 1988 EMJ (Ewha medical journal) Vol.11 No.3

Recently, the present authors obtained 6 worms (4 females and 2 males) from both eyes of a farmer aged 23. Worms were fixed in 10% formalin, examined, and measured. All of worms were identified as Thelazia callipaeda Railliet and Henry, 1910. The findings of parasitological studies on 21 specimens (12 females and 9 males) removed from 12 cases of human thelaziasis already described in Korea were reviewed together with our findings. And percentages of lengths of main structures to the body length of each worm were also calculated for comparison. Female and male specimeus measured 14.31 and 10.93mm in length, 0.42(2.9%) and 0.34mm(2.8%) in diameter, 0.03(0.2%) in length of buccal cavity, 0.70(5.1%) and 0.56mm(5.2%) in distance from anterior end to esophago-intestinal junction, and 0.08(0.5%) and 0.05mm(0.5%) in length from posterior end to anus or cloaca in average, respectively. Vaginal openings in all of female were located about0.2mm anteriorly to esophago-intestinal junctions. Minimum and maximum in male worms, respectively. In our 4 female specimens only a pair of processes at terminal of the tail were demonstrated, while in 2 males 8 pairs of precloacal and 3 pairs of postcloacal papillae were noted. Some wrinkle-like structures were observed on ventral and dorsal surfaces in posterior portion of a male specimen.

질소산화물 제거를 위한 디스크형 바나디아 촉매담지 세라믹필터의 특성

홍민선,문수호,이재춘,이동섭 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.4

The performance of disk-type catalytic fillers impregnated by TiO₂ or TiO₂-3Al₂O₃ㆍ2SiO₂ supports and V₂O_(5), catalyst was evaluated for selective catalytic reduction (SCR) of NO with ammonia as a reductant. XRD, FT-IR, BET and SEM were used to characterize the catalytic filters prepared in this work. Optimal V₂O_(5) loading and reaction temperature for V₂O_(5)/TiO₂ catalytic filters were 3~6wt.% and 350~400℃ at GHSV 14.300hr-¹ in the presence of oxygen, respectively. With increasing the V₂O_(5) loading from 0.5 to 6 wt.%, NO conversion increased from 24 to 96% at 400℃ and 14.300 hr-¹, and maintained at 80% over in the V₂O_(5) loading range of 3~6 wt.% and then dropped at V₂O_(5) loading of 7wt.% over. In comparing V₂O_(5)/TiO₂ and V₂O_(5)/TiO₂-3Al₂O₃ㆍ2SiO₂ catalytic fillers, which have same 3 wt.% V₂O_(5) loading, the V₂O_(5)/TiO₂-3Al₂O₃ㆍ2SiO₂ catalytic filter showed higher activity than V₂O_(5)/TiO₂ catalytic filter, but higher differential pressure drops owing to its low air permeability.

디스크형 산화구리 촉매담지 세라믹필터의 제조와 물성

홍민선,문수호,이재춘,이동섭,임우택 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.2

A catalyst with CuO ceramic filter for simultaneous treatment of dust and HAP was prepared and characterized. Catalytic ceramic tilter can not only potentially achieve the substantial savings in energy but provide with effective optimization and integration of process for simultaneous removal of S0₂ㆍ NOx and particulates from flue gases. Catalytic ceramic filters remove simultaneously particulates on exterior surface of filters and reduce NO to N₂and H₂O by SCR (Selective Catalytic Reduction) process. Preparation of catalyst impregnated ceramic filter with disk shape (Ψ50) follow the processing of alumino-silicate ceramic filter, support impregnation and catalyst impregnation(copper oxide). Preparation routes of alumino-silicate catalyst carrier suitable for production of catalytic filters practically were studied and developed using the sol-gel and colloidal processing, homogeneous precipitation and impregnation method. Characterization of the catalyst, catalyst carrier catalytic filter materials have been performed the using various techniques such as BET, XRD, TGA, SEM. Combination of the sol-gel and colloidal processing and impregnation method is recommended to prepare catalyst carriers economically for catalytic filter applications.

마우스에 있어서의 肝吸蟲 감염에 대한 조직비만세포의 반응

민홍기,전계식 梨花女子大學校 醫科大學 醫科學硏究所 1994 EMJ (Ewha medical journal) Vol.17 No.3

BALB/c mice were used and divided into 2 experimental groups ; group 1, infected with a single dose of 50 Clonorchis matacercariae and group 2, challenged with 50 metacercariae at the 4th week after primary infection with the same dose of metacercariae. Normal mice were served as control. Three mice in each group were examined weekly for 10 weeks. Mice in groups 1 and 2 were sacrifficed. Liver including gall bladder and common bile duct was group were intraperitoneally injected with ethanol and fixed. The liver including common bile and sectioned serially and stained with toluidine blue for examination of appearance degree of mast cells. The medenterium was used for examination of appearance degree of mast cells and percentage of degranulating cells. The results obtained in this study are summarised as following: 1) Average number of worm recovered was 13.1 in group 1 and 15.7 in group 2, respectively, throughout the examination. Mean number by week after reinvection in group 2 revealed a slight increase, ranging from 3.0 to 9.0, comparing with those in group 1. 2) In control mast cells were found in the mesenterium, but not in the bile duct, small intestine and lymphnodes. No mast cells were found in the small intestine in groups 1 and 2. In group 1 nast cells showed a moderate appearance degree at the 3rd week and persisted for 2-4 weeks in the bile dust, mesenterium and lymphnodes. Group 2 shoeed a moderate or marked degree ehich persisted for longer period after reinfection in the tissues. 3) Average percentage of degranulating cells in the mesenterium was 9.1% in control and 26.3% in froup 1, respectivley, throughout the examination and 51.7% during 6 weeks after reinfection in group 2. The peak was 36.7% at the 3rd week after primary infection in groups 1 and 2. In group 2, the percentage of degranulating cells increased promptly and markedly to 77.3% at the 1st week after reinfection. 4) Chronological of appearance degree of mast cells and percentage of degranulating cells in the tissues, particularly in the bile duct showed an obvious tendency to parallel to chronological change of number of worm recovered in the liver. From the adove results, it is strongly suggested that mast cells in mice infected with Clonorchis sinensis are deeply implicated in expulsion of worms in bile dust.

산화구리 촉매담지 세라믹 캔들필터를 이용한 NO 제거

홍민선,문수호,이재춘,이동섭 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.3

The CuO/3Al₂O₃ㆍ2Si0₂ catalyst impregnated ceramic candle filters for nitrogen oxides removal were prepared by porous mullite(3Al₂O₃ㆍ2Si0₂) support and CuO catalyst deposited on this support to achieve uniformly dispersed CuO deposition, which arc impregnated into the pores of available alumino-silicate ceramic candle filter. The CuO/3Al₂O₃ㆍ2Si0₂ catalyst impregnated ceramic candle filters were characterized by XRD, BET, air permeability, pore size, SEM and catalytic tests in the reduction of NOx by NH₃. The observed effects of CuO/3Al₂O₃ㆍ2Si0₂ impregnated ceramic candle tilters in SCR reaction arc as follows: (1) when the content of CuO catalyst increased further, activity of NO increased. (2) NO conversion at first increased with temperature and then decreased at high temperatures (above 400℃), possibly due to the occurrence of the ammonia oxidation reaction. (3) In pilot plant test for 3 months, NO conversion was greater than 90%.

江華郡內 肺吸蟲症 濃厚蔓延地域에 있어서의 疫學的 樣相의 變化

閔弘基,田桂植 梨花女子大學校 醫科大學 醫科學硏究所 1987 EMJ (Ewha medical journal) Vol.10 No.4

In Korea, paragonimiasis is one of the most important endemic parasitic diseases with significant clinical relvance. Since 1917 when Kobayashi has reported a high infection rate(88.0%) of paragonimus metacercariae among crayfish, an important intermediate host, Kanghwa-gun has been known to be a hyperendemic region of paragonimiasis. The present authors carried out an epidemiologic studies to obtain some current informations on the change in epidemiologic aspects of paragonimiasis in Giljeong-ri, Yangdo-myun, Kanghwa-gun, the most hyperendemic region. The infection status of Paragonimus metacercariae in crayfish have been examined. And a total of 392 students of Kangnam Middle School was examined by the intradermal test with Paragonimus antigen. In order to detect Paragonumus egg from the positive reactors, the fecal and sputum examinations were performed. As shown in Tables 1 and 2, there are marked reducing tendencies in the infection rate in crayfish and in the average number of metacercariae per crayfixh examined; 66.2% and 17.2 in 1981, 59.6% and 8.4 in 1984 and 20.0% and 0.6% in 1987, respectively. According to Table 3, 7.1% of the students showed positive reaction to Paragonimus autigeu with the sexual distribution of 8.0% in male and 6.0% in female. However, no Paragonimus eggs were found from the positive reactors by the fecal and sputum examinations.

개蛔蟲 幼蟲 感染 마우스에 있어서의 宿主 低抗性

閔弘基,田桂植 梨花女子大學校 醫科大學 醫科學硏究所 1991 EMJ (Ewha medical journal) Vol.14 No.2

A series of experiments were carried out to obtain some informations on host resistance in mice infected with Toxocara canis. Approximately 210 male BLAB/c mice were used and given various doses of infective eggs. For the purpose. the mean percentages of eosinophils were counted. and recovery rates of larvae from the liver, lung, brain, carcass and other tissues were examined separately at various intervals according to the experimental schedules. Experiment Ⅰ: Mice in groups Ⅰ-1 and Ⅰ-2 were given a single dose of 30 eggs and 100 eggs, respectively. Eosinophils and larvae were examined daily for 6 days and weekly for 21 weeks. Experiment Ⅱ : Mice in groups Ⅱ-1 and Ⅱ-2 were given 100 eggs weekly for 2 weeks and 50 eggs for 4 weeks from 1 week after an initial administration of 30 eggs. Eosinophils were counted weekly and larvae were examined at 3 weeks after the final administration, respectively. Experiment Ⅲ : Mice in 6 groups from Ⅲ-1 to Ⅲ-6 were given a challenge dose of 100 eggs at 1. 2. 3. 5. 7, and 9 weeks after an initial administration of 30 eggs. Eosinophils were counted weekly for 3 weeks and larvae were examined at the 3rd week after the challenge infection, respectively. The means of eosinophils in groups Ⅰ-1 nd Ⅰ-2 respectively were 6.4% and 8.2% at 6 hr. and reached a maximum peak of 16.8% and 21.6% at 2 weeks, and the means decreased rapidly for the next 6 weeks. then fell gradually. The superinfection and the challenge infection in Experments Ⅱ and Ⅲ caused quicker and more intense rises of the eosinophils. The recovery rates of larvae in groups Ⅰ-1 and Ⅰ-2 respectively showed 3 peaks at 48 hr. 1 week and 3 weeks, then decreased gradually. At 48 hr. 88.2% and 93.5% of the larvae recovered and 38.0% and 40.8% of the total larvae given were found in the liver, then decreased gradually until none was found at the end of the experiment. On the contrary. in the brain and carcass, the larvae accuulated repidly through the 1st week and the rates persisted up to the 8th week without remarkable variation. The recovery rates of larvae in total, and from the brain and carcass in groups of Experiments Ⅱ and Ⅲ decreased remarkably, whereas those from the liver increased significantly compared with groups Ⅰ-1 and Ⅰ-2 at the corresponding weeks of examination. Transition patterns of the means of eosinophils in groups of Experiments Ⅰ and Ⅱ and those of total recovery rates of larvae in groups of Experiment I were essentially similar each other in rise and fall throughout the experiments. From the overall results, it is strongly suggested that eosinophils do affect the development of resistance to invading Toxocara larvae, and the higher recovery rates of larvae in the liver indicates that the liver is the organ which may play a role of protection against infection with T. canis larvae by means of immunomechanism in mice.