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콩에서 발생하는 강낭콩일반모자이크바이러스(BCMV)와 땅콩위축바이러스(PSV)의 저항성 평가
이영훈, 윤영남, 강범규, 김현영, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
It has been reported several viral diseases in soybean. The occurrence of Bean common mosaic virus and Peanut stunt virus are able to induce severe problem because the main breeding target for viral disease was focused on Soybean mosaic virus. BCMV is a spcies of the genus Potyvirus of the family Potyviridae. BCMV reduces yield by as much as 80% and also adversely affects seed quality in common bean. BCMV has a worldwide distribution because of its high rates 35% of transmission via seeds produced by plants systemically infected prior to bloom. PSV is a species of the genus Cucumovirus of the family Bromoviridae. PSV has been reported to be economically damaging on beans worldwide. Although the virus is reportedly seed transmissible at very low rates, infected plants rarely produce pods with normal viable seeds. The most satisfactory management method of viral diseases such as BCMV and PSV is the use of resistant cultivars and healthy seed. For the overcome of genetic resistance by new virus such as BCMV and PSV, virus diseases monitoring and researches need to study deeply and continually. In this study, we conducted the resistance screen on BCMV and PSV for selection of resistance cultivars. We inoculated on 128 cultivars with PSV and BCMV respectively. These cultivars were classified through pathogenic response such as resistance, necrosis and susceptibility. In addition, 99 and 66 resistant cultivars were confirmed against PSV and BCMV respectively.
RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
Genetic and Environmental Variation of First Pod Height in Soybean [Glycine max (L.) Merr.]
( Beom-kyu Kang ),( Hyun-tae Kim ),( Man-soo Choi ),( Seong-chul Koo ),( Jeong-hyun Seo ),( Hong-sik Kim ),( Sang-ouk Shin ),( Hong-tae Yun ),( In-seok Oh ),( Krishnanand P. Kulkarni ),( Jeong-dong Le 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.1
First pod height (FPH) is an agronomic trait for the mechanical harvesting of soybeans with combines. The seed loss could be minimized, if the FPH is higher than the height of the cutter bar in combines. Hence, developing soybeans with high FPH has become one of important breeding goals in current crop improvement programs. The objective of this study was to evaluate genetic and environmental variation of FPH in soybean and to analyze the effect of ratio of FPH to plant height (PH) on seed yield. Four genotypes were evaluated across six different environments to analyze environmental variation of agronomic traits including FPH. Three F<sub>2</sub> populations were evaluated to analyze genetic variation and relationship between the ratio of FPH to PH and seed yield. The main effects of planting distance, genotype and seeding date were significant for FPH, but FPH is affected more by genetic factors than by environmental factors. The mean heritability value of FPH was 66% across three F<sub>2</sub> populations. Seed yield was found to reduce with increase in the FPH/PH ratio. In conclusion, genetic factors have effect more than environments to the variation of FPH. While FPH is higher than cutting height, the smaller ratio can minimize seed yield decrease.
쓰러짐에 강하고 알이 작아 콩나물 수율이 높은 나물용 콩 품종 ‘해원’
강범규(Beom Kyu Kang),서정현(Jeong Hyun Seo),김홍식(Hong Sik Kim),김현태(Hyun Tae Kim),이영훈(Young Hoon Lee),이병원(Byung Won Lee),최만수(Man Soo Choi),하태정(Tae Joung Ha),고종민(Jong Min Ko),윤홍태(Hong Tai Yun),백인열(In Youl Bae 한국육종학회 2021 한국육종학회지 Vol.53 No.1
‘Haewon’ is a soybean cultivar for soy-sprouts. ‘Bosug’ and ‘Somyeong’ were crossed in 2003, and promising lines were selected from F3 to F6 using the pedigree method (Pedigree: HS1450-2B-3-1-1-2-2-1). Preliminary yield (PYT) and advanced yield trials (AYT) were conducted from 2010 to 2011, and regional yield trials (RYT) were conducted in five regions (Suwon, Naju, Miryang, Dalseong, and Jeju) from 2012 to 2014. In the RYTs, ‘Haewon’ had a smaller seed weight and higher yield than the standard cultivar ‘Pungsannamulkong’, which are desirable characteristics for the soy-sprout process. The morphological characteristics of ‘Haewon’ are as follows: determinate plant type, purple flowers, gray pubescence, yellow pods, small, yellow spherical seeds (8.1 g/100-seeds), and light brown hilum. The flowering and maturity dates were July 29th and October 6th, respectively. Plant height and first pod height were 55 cm and 12 cm, respectively. The lodging tolerance was also strong compared to that of ‘Pungsannamulkong’. The germination rate, total length, hypocotyl length, thickness, and sprout yield of soybean sprouts were 97%, 19.1 cm, 8.8 cm, 2.1 mm, and 513%, respectively. The yield in adaptable regions was 337 kg/10 a, 16% higher than that of ‘Pungsannamulkong’. ‘Haewon’ can be used for soy-sprout with favorable seed size and soy-sprout characteristics, such as germination and sprout-yield. (Registration No. 6637).
2모작 적응 준조숙 내탈립 대립 다수성 콩 품종 ‘선유2호’
강범규(Beom Kyu Kang),서정현(Jeong Hyun Seo),김현태(Hyun Tae Kim),백인열(In Youl Baek),최만수(Man Soo Choi),박장환(Chang Hwan Park),윤홍태(Hong Tae Yun),신상욱(Sang Ouk Shin),김홍식(Hong Sik Kim),곽도연(Do Yeon Gwak),고지연(Ji Yeon K 한국육종학회 2022 한국육종학회지 Vol.54 No.4
A soybean cultivar, “Seonyu2ho” for double cropping using soy-paste and tofu was developed in 2011 using the pedigree method by crossing “Milyang235ho” and “Jungmo3004ho.” A promising line, YS2441-B-2S-11-3, was selected and named as “Milyang334.” It demonstrated prominent early maturing, shattering resistance, and high yield in regional yield trials (RYTs) from 2017 to 2019, and was released by the name “Seonyu2ho.” The cultivar showed a determinate growth habit, white flowers, grey pubescence, yellow seed coat, yellow hilum, flat-spherical seed shape, and large seed size (27.9 g per 100 seeds). “Seonyu2ho” matured on October 5 (112-growing day cycle) in RYT and was suitable for double cropping with winter crops, such as wheat, barley, and onion. It was resistant to bacterial pustule and s oyb ean mosaic virus and tolerant to lodging in fields. Particularly, “Seonyu2ho” showed a high yield (3.34 ton/ha) and shattering resistance, even if the previously developed early matured cultivars exhibited low yield and easy shattering. Overall, “Seonyu2ho” can contribute to stable soybean production in double cropping.
고 감속 차동 복합 유성기어 감속 시스템의 구조 안전성 분석
강범준(Beom-jun Kang),유영락(Young-Rak Yoo),박규태(Kyu-Tae Park),김재범(Jae-Beom Kim),정동헌(Dong-Heon Jeong),신슬기(Sul-Gi Shin),이지환(Ji-Hwan Lee) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.4
해상 물류 시스템 장비, 대형 산업 장비 및 건설 중장비의 높은 초기 기동 토크에 대응하기 위해 유압식 감속 시스템 모듈이 적용되고 있다. 감속 시스템의 구조는 회전력을 발생하는 유압 모터와 회전하는 구동 축을 감속하는 유압 브레이크, 그리고 회전력을 일정한 비율로 감속하고 동력을 전달하는 유성기어 감속기로 구성된다. 본 연구에서는 기존의 다단 유성기어 감속 방식에서 10%이상의 부품 수 감소와 5% 이상의 소형화 및 경량화가 가능한 차동 복합 유성 감속기를 제시하고 있으며 설계 조건으로 DNV Standard 2.22 규격을 적용하였다. 감속 시스템 기어 류에 대해 Lewis 와 Hertz 이론 기반의 굽힘 응력과 면압 응력을 산출하였고 상용 FEM 구조 해석 프로그램을 통해 구조적 안전성을 분석하였다. In order to cope with the high initial starting torque of marine logistics system equipment, large industrial equipment, and heavy construction equipment, hydraulic reduction system modules are being applied. The structure of the reduction system is composed of a hydraulic motor that generates rotational force, a hydraulic brake that decelerates the rotating drive shaft, and a planetary gear reducer that decelerates the rotational force at a constant rate and transmits power. In this study, a differential compound planetary reducer capable of reducing the number of parts by more than 10%, miniaturization and weight reduction by more than 5% in the conventional multi-stage planetary gear reduction method is proposed, and DNV Standard 2.22 standard is applied as a design condition. Bending stress and surface contact stress based on Lewis and Hertz theory were calculated for gears in the reduction system, and structural safety was analyzed through a commercial FEM structural analysis program.
Jeon, Beom Su,Shin, Byung Ho,Huh, Beom Kang,Kim, Byung Hwi,Kim, Se-Na,Ji, Han Bi,Lee, Seung Ho,Kang, Soo Im,Shim, Jung Hee,Kang, So Min,Lee, Jae Chul,Lee, Kyu Sang,Heo, Chan Yeong,Choy, Young Bin Elsevier 2018 Journal of industrial and engineering chemistry Vol.63 No.-
<P><B>Abstract</B></P> <P>We propose silicone implants capable of the local, controlled release of a glucocorticoid drug, triamcinolone acetonide (TA), for the prevention of fibrosis. The shells of these silicone implants were coated with two different loading amounts of TA, which could release the drug in a sustained manner for 12 weeks. The drug-loaded implants were inserted into the subcutaneous space in living rats, and the tissues were biopsied at scheduled times during 12 weeks. For the drug-coated implants, the capsule thickness and collagen density decreased compared with those of the non-coated implant. Because of the effect of TA, inflammation and the expression of pro-inflammatory cytokines were downregulated, thereby decreasing the number of monocytes during acute inflammation. This effect in turn decreased the number of macrophages at the later stage of inflammation, leading to the expression of less TGF-β and consequently fewer fibroblasts and myofibroblasts. Our findings also revealed that with an appropriate dose control, skin and muscle atrophy, major side effects of TA, could be avoided while still effectively reducing fibrosis. Therefore, we conclude that the local, sustained release of an appropriate dose of a glucocorticoid drug can be a promising strategy for safely preventing fibrosis around silicone implants.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>