Generation of Antigen Specific Murine T Cell Lines and Clones

Bai,Gill Han 서울大學校保建大學院 1993 國民保健硏究所硏究論叢 Vol.3 No.1

각종 T cell line 과 clone 을 확립하기 위한 방법이 개발됨에 따라 면역학적 반응에 대한 이해와 면역학 전반 분야의 발전이 크게 촉진되었다. 그러나 면역학적 연구에 가장 기본적이고도 중요하게 이용되는 이 방법이 실제로는 실험실마다 차이를 보이고 있어 표준화를 통한 기술 접근을 용이하게 함이 절실하였다. 본 논문에서는 T cell line 과 clone 을 얻기위해 가장 보편적이면서도 비교적 편리하게 쓰일 수 있는 방법을 제시함으로써, T cell 관련 연구자들에게 도움을 주고자 하였다. Within the field of T cell approach an armamentarium of methods is accumulating and has already been appiled on fairly extensive scale. However, these methods are still under intensive developments, and there is yet little agreement on the methods which are appropriate for particular problems. Author attempted to describe the existing techniques requried for generation of murine T cell and cloes with specific antigen reactivity in vitro. Optimal conditions for individual laboratories and experiments must be derived empirically with respect to variable conditions, but suggested techniques in this paper may be commonly useful in most laboratories.

제품군의 재사용 가능한 클론 코드의 메소드 경로 통일을 위한 코드 클러스터링 방법

김태영 ( Kim Taeyoung ),이지현 ( Lee Jihyun ),김은미 ( Kim Eunmi ) 한국정보처리학회 2023 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.12 No.1

유사한 소프트웨어는 기존 산출물을 복제하고 수정하는 클론-앤-오운(clone-and-own, CAO) 방법으로 개발되곤 한다. 그러나 클론-앤-오운 방법은 복제된 제품의 수가 늘면서 유지보수를 어렵게 만들기 때문에 나쁜 프랙티스로 간주된다. 소프트웨어 제품라인 공학은 체계적인 재사용을 통해 소프트웨어 제품군을 개발하는 방법으로 클론-앤-오운 방법의 문제를 해결할 수 있다. CAO 방식으로 개발되어 온 제품패밀리를 제품라인 공학으로 마이그레이션하는 작업은 여러 소프트웨어 제품에서 클로닝된 부분들을 찾아 통합하고 재사용 가능한 자산으로 구축하는 것으로부터 시작된다. 그러나 클로닝이 디렉토리부터 코드 라인까지 다양한 수준에서 발생하고 그 과정에서 이들의 구조에 변경이 일어날 수 있어 단순하게 클로닝을 찾아내는 것만으로는 고품질의 제품라인 코드베이스를 구축하기 어렵다. 성공적인 마이그레이션을 위해서는 소스 코드들 사이의 클로닝 관계를 찾는 것 이외에도 소스 코드들의 파일 경로와 클래스 이름, 메소드 시그니처 등의 동일성을 확보는 작업이 선행되어야 한다. 이에 본 연구는 CAO 기반으로 개발된 제품들로부터 마이그레이션 대상 제품들을 선정한 후 제품들에 흩어져 있는 유사 코드 집합을 검출하여 메소드 경로의 통일이 필요한 대상을 식별하는 클러스터링 방법을 제안한다. 제안 방법의 효과를 보이기 위해 CAO 방식으로 진화해온 ApoGames 제품군에 제안 방법을 적용하여 실험을 진행하였다. 그 결과, 전처리 없이 수행된 파일의 상대 경로 기반 클러스터링 방법의 평균 정밀도는 0.91이며 식별된 공통 클러스터의 개수는 0개인 반면에 이 논문에서 제안하는 전처리와 함께 수행된 메소드 시그니처 기반 클러스터링 방법의 평균 정밀도는 0.98로 개선되었으며 식별된 공통 클러스터는 최대 15개까지 증가하였다. Similar software is often developed with the Clone-And-Own (CAO) approach that copies and modifies existing artifacts. The CAO approach is considered as a bad practice because it makes maintenance difficult as the number of cloned products increases. Software product line engineering is a methodology that can solve the issue of the CAO approach by developing a product family through systematic reuse. Migrating product families that have been developed with the CAO approach to the product line engineering begins with finding, integrating, and building them as reusable assets. However, cloning occurs at various levels from directories to code lines, and their structures can be changed. This makes it difficult to build product line code base simply by finding clones. Successful migration thus requires unifying the source code's file path, class name, and method signature. This paper proposes a clustering method that identifies a set of similar codes scattered across product variants and some of their method full paths are different, so path unification is necessary. In order to show the effectiveness of the proposed method, we conducted an experiment using the Apo Games product line, which has evolved with the CAO approach. As a result, the average precision of clustering performed without preprocessing was 0.91 and the number of identified common clusters was 0, whereas our method showed 0.98 and 15 respectively.

Cell cloning-on-the-spot by using an attachable silicone cylinder

Park, H.B.,Son, W.,Chae, D.H.,Lee, J.,Kim, I.W.,Yang, W.,Sung, J.K.,Lim, K.,Lee, J.H.,Kim, K.H.,Park, J.I. Academic Press 2016 Biochemical and biophysical research communication Vol.474 No.4

Cell cloning is a laboratory routine to isolate and keep particular properties of cultured cells. Transfected or other genetically modified cells can be selected by the traditional microbiological cloning. In addition, common laboratory cell lines are prone to genotypic drift during their continual culture, so that supplementary cloning steps are often required to maintain correct lineage phenotypes. Here, we designed a silicone-made attachable cloning cylinder, which facilitated an easy and bona fide cloning of interested cells. This silicone cylinder was easy to make, showed competent stickiness to laboratory plastics including culture dishes, and hence enabled secure isolation and culture for days of selected single cells, especially, on the spots of preceding cell-plating dishes under microscopic examination of visible cellular phenotypes. We tested the silicone cylinder in the monoclonal subcloning from a heterogeneous population of a breast cancer cell line, MDA-MB-231, and readily established independent MDA-MB-231 subclones showing different sublineage phenotypes.

자기방사능법에 의한 DNA in situ Hybridization방법

장성익,양창헌,김대광,정용욱,최인장,이인환 계명대학교 의과대학 1991 계명의대학술지 Vol.10 No.2

To find out the optimal condition for DNA in situ hybridzation using isotopically labeled DNA probe with the general goal of understanding how localization and organization of N-myc oncogene sequences within the SK-N-BE cell line, present investigation was carried out by ³H-labeled pMF3-SEBI-Nmyc probe in situ hybridization. The results were as follows: Specific activities of 2~5×10??? dpm/㎍ of DNA were gained after separation of the ³H labeled DNA by spindialysis using sepharose CL-6B. The chromosomes and interphase nuclei were hybridization with the labeled probe for 14hr at 41℃ in a hybridization mixture solution: 50% formamide, 2×SSCP(2×SSC, 0.04M NaPO4, pH7.0), 10% dextran sulfate, 100㎍/ml of sonicated salmon sperm DNA and 0.2mg/ml each of bovine serum albumin, Ficoll, and polyvinylphyrrolidone. Autoradiography was performed by using half-strength Sakura NR-M2 emulsion for 7-14 days at 4℃. Silver grains were simultaneously detected on Q-banded chromosomes in the same metaphase. Amplifications of N-myc oncogene were conformed on homogeneous staining regions of chromosome 11 and marker chromosome by statistical analysis. It was impossible with this technique to localize N-myc oncogene within the interphase nucleus of SK-N-BE cell line.

Every Single Cell Clones from Cancer Cell Lines Growing Tumors In Vivo May Not Invalidate the Cancer Stem Cell Concept

Fengzhi Li 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.4

We present the result of our research on the tumorigenic ability of single cell clones isolated from an aggressive murine breast cancer cell line in a matched allografting mouse model. Tumor formation is basically dependent on the cell numbers injected per location. We argue that in vivo tumor formation from single cell clones, isolated in vitro from cancer cell lines, may not provide conclusive evidence to disprove the cancer stem cell (CSC) theory without additional data.

Korean Medicinal Herb Extracts Inhibit Melanin Formation in Clone M-3 Mouse Melanocyte Cell Lines

( Kap Joo Park ),( Dong Wan Choo ),( Hyung Hoan Lee ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.2

N/A In order to search for anti-melanin formation agents from Korean medicinal herbs, we selected 21 Korean medicinal herbs, based on a review of Korean traditional medicine books and the recommendations of Korean traditional medical doctors. We tested for inhibition effect of melanin pigmentation of Clone M-3 mouse melanocyte cell lines when we treated the extracts of 21 medicinal herbs in the mouse melanocyte cell lines, respectively. Among 21 medicinal herb extracts, 5 extracts showed a inhibition effect of melanin formation. The sample Phaseolus radiatus L, Cordyceps militaris, Pinellia ternata, Phellinus linteus and Citrus junos Tanaka showed a significantly little formation of melanin pigments compared with control groups. Especially extract of Citrus junos Tanaka was more potent than the others. These results suggest that extract of Korean Citrus junos may represents an excellent candidate for inhibition of melanin pigmentation at in vitro level.

Use of Flp-Mediated Cassette Exchange in the Development of a CHO Cell Line Stably Producing Erythropoietin

( Min Soo Kim ),( Gyun Min Lee ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.7

청정해역 곰치추출물의 세포생리활성 연구

황은경 ( Eun Kyoung Whang ),조명환 ( Myung Hwan Cho ),박찬선 ( Chan Sun Park ),김명희 ( Myung Hee Kim ),박갑주 ( Kap Joo Park ) 한국환경생물학회 2008 환경생물 : 환경생물학회지 Vol.26 No.1