Quantitative Light-Induced Fluorescence-Digital을 이용한 치면세균막 검사법의 임상적 활용 가능성 평가

황혜림 ( Hye Rim Hwang ),조영식 ( Young Sik Cho ),김백일 ( Baek Il Kim ) 한국치위생과학회 2014 치위생과학회지 Vol.14 No.2

The aim of this study was to suggest a convenient method of monitoring the gingival state through plaque detection. Quantitative Light-Induced Fluorescence-Digital (QLF-D), which can assess mature plaque, can be used to assess the oral hygiene status of individuals and to establish an adequate intervention plans for them. The subjects of the study participated in the clinical training at Department of Dental Hygiene, N University. The subjects completed questionnaires on general characteristics and oral hygiene methods. Then, photographs of maxillary and mandibular anterior teeth of the subjects were taken using the QLF-D. After the oral examinations, gingival state was recorded according to the Loe & Silness`s Gingival Index (GI). In addition, a plaque control record was calculated in percentage using disclosing agent. The analysis of the relation between the plaque control record and the QLF-D scores showed positive correlation (r=0.638, p<0.001), and the analysis of the relation between the QLF-D scores and the gingival bleeding index scores also showed positive correlation (r=0.562, p<0.001). Besides, the study classified the participants into healthy gingiva group and the gingivitis group according to the classification criteria of GI, and when the difference of QLF-D scores between the groups was analyzed, the QLF-D scores were statistically significantly higher (t=-2.785, p=0.007) in the gingivitis group (1.71±1.545) than the healthy gingiva group (0.74±1.290). When the differences in mean values of the QLF-D scores were analyzed against and the gingival bleeding index scores, the QLF-D scores of 0 and 1 belonged to the category of normal gingival state while the QLF-D scores of 2, 3 and 4 belonged to the category of gingivitis state. Therefore, it is deemed that the red fluorescence detected by the QLF-D from the mature supragingival plaque can be used for monitoring the state of gingivitis.

Qraycam을 이용한 치면세균막 검사의 타당도와 신뢰도

김미 ( Mi Kim ),이수영 ( Su Young Lee ),조영식 ( Young Sik Cho ) 한국치위생과학회 2015 치위생과학회지 Vol.15 No.3

The purpose of this study was to evaluate the validity and reliability of plaque scoring system using new Qraycam (All in One Bio, Korea) device which enables plaque score without tooth disclosing. This study measured Quigley-Hein index and plaque control record by both Qraycam and disclosing agent on 64 elderly people and checked degree of congruence between the two methods. Reliability was evaluated with the mean of measured values, kappa index and intraclass correlation coefficient statistical analysis. The analysis of the plaque scores showed a high agreement between the measured values according to the method of measurement and the measured part. The mean of plaque index of anterior labial were not significantly different according to measurement method. The kappa index was higher by Qraycam and tooth disclosing method of plaque index. Therefore, it was verified that Qraycam has sufficient reliability as screening tool for plaque scoring system.

제지용 형광증백제의 열 견뢰도에 대한 연구

이지영(Ji-Young Lee),김철환(Chul-Hwan Kim),이희진(Hui-Jin Lee),곽혜정(Hye-Joeng Gwak) 한국펄프·종이공학회 2010 한국펄프·종이공학회 학술발표논문집 Vol.2010 No.10

Fluorescent whitening agents (FWAs) are used extensively in the paper industry to improve the optical properties. FWAs are neither photochemically nor thermally stable, and thereby the papers containing FWAs can be damaged by external heat. We observed that the CIE whiteness, ISO brightness and fluorescence index of the commercial papers decreased with the thermal treatment and thought that the thermal stability of FWAs was the main factor affecting the thermal fastness of the papers. Thus, the model papers treated with three types of FWAs were manufactured respectively and the thermal stability of FWAs was identified by determining Δ CIE whiteness and Δ fluorescence index.

Photochemical Response Analysis on Drought Stress for Red Pepper (Capsiumannuum L.)

Yoo, Sung-Yung,Lee, Yong-Ho,Park, So-Hyun,Choi, Kyong-Mi,Park, June-Young,Kim, A-Ram,Hwang, Su-Min,Lee, Min-Ju,Ko, Tae-Seok,Kim, Tae-Wan 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

The aim of this study is to determine the drought stress index through photochemical analysis in red pepper (Capsiumannuum L.). The photochemical interpretation was performed in the basis of the relation between Kautsky effect and Photosystem II (PSII) following the measurement of chlorophyll, pheophytin contents, and $CO_2$ assimilation in drought stressed 5-week-old red pepper plants. The $CO_2$ assimilation rate was severely lowered with almost 77% reduction of chlorophyll and pheophytin contents at four days after non-irrigation. It was clearly observed that the chlorophyll fluorescence intensity rose from a minimum level (the O level), in less than one second, to a maximum level (the P-level) via two intermediate steps labeled J and I (OJIP process). Drought factor index (DFI) was also calculated using measured OJIP parameters. The DFI was -0.22, meaning not only the initial inhibition of PSII but also sequential inhibition of PSI. In real, most of all photochemical parameters such as quantum yield of the electron transport flux from Quinone A ($Q_A$) to Quinone B ($Q_B$), quantum yield of the electron transport flux until the PSI electron acceptors, quantum yield of the electron transport flux until the PSI electron acceptors, average absorbed photon flux per PSII reaction center, and electron transport flux until PSI acceptors per cross section were profoundly reduced except number of QA reducing reaction centers (RCs) per PSII antenna chlorophyll (RC/ABS). It was illuminated that at least 6 parameters related with quantum yield/efficiency and specific energy fluxes (per active PSII RC) could be applied to be used as the drought stress index. Furthermore, in the combination of parameters, driving forces (DF) for photochemical activity could be deduced from the performance index (PI) for energy conservation from photons absorbed by PSII antenna until the reduction of PSI acceptors. In conclusion, photochemical responses and their related parameters can be used as physiological DFI.

Photochemical Response Analysis on Drought Stress for Red Pepper (Capsiumannuum L.)

Sung-Yung Yoo,Yong-Ho Lee,So-Hyun Park,Kyong-mi Choi,June-Young Park,A-Ram Kim,Su-Min Hwang,Min-Ju Lee,Tae-Seok Ko,Tae-Wan Kim 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

The aim of this study is to determine the drought stress index through photochemical analysis in red pepper (Capsiumannuum L.). The photochemical interpretation was performed in the basis of the relation between Kautsky effect and Photosystem II (PSII) following the measurement of chlorophyll, pheophytin contents, and CO₂ assimilation in drought stressed 5-week-old red pepper plants. The CO₂ assimilation rate was severely lowered with almost 77% reduction of chlorophyll and pheophytin contents at four days after non-irrigation. It was clearly observed that the chlorophyll fluorescence intensity rose from a minimum level (the O level), in less than one second, to a maximum level (the P-level) via two intermediate steps labeled J and I (OJIP process). Drought factor index (DFI) was also calculated using measured OJIP parameters. The DFI was -0.22, meaning not only the initial inhibition of PSII but also sequential inhibition of PSI . In real, most of all photochemical parameters such as quantum yield of the electron transport flux from Quinone A (QA) to Quinone B (QB), quantum yield of the electron transport flux until the PSI electron acceptors, quantum yield of the electron transport flux until the PSI electron acceptors, average absorbed photon flux per PSII reaction center, and electron transport flux until PSI acceptors per cross section were profoundly reduced except number of QA reducing reaction centers (RCs) per PSII antenna chlorophyll (RC/ABS). It was illuminated that at least 6 parameters related with quantum yield/efficiency and specific energy fluxes (per active PSII RC) could be applied to be used as the drought stress index. Furthermore, in the combination of parameters, driving forces (DF) for photochemical activity could be deduced from the performance index (PI) for energy conservation from photons absorbed by PSII antenna until the reduction of PSI acceptors. In conclusion, photochemical responses and their related parameters can be used as physiological DFI.

Photochemical Response Analysis on Drought Stress for Red Pepper (Capsiumannuum L.)

유성녕,이용호,박소현,최경미,박준영,김아람,황수민,이민주,고태석,김태완 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

The aim of this study is to determine the drought stress index through photochemical analysis in red pepper(Capsiumannuum L.). The photochemical interpretation was performed in the basis of the relation betweenKautsky effect and Photosystem II (PSII) following the measurement of chlorophyll, pheophytin contents, andCO2 assimilation in drought stressed 5-week-old red pepper plants. The CO2 assimilation rate was severelylowered with almost 77% reduction of chlorophyll and pheophytin contents at four days after non-irrigation. It was clearly observed that the chlorophyll fluorescence intensity rose from a minimum level (the O level), inless than one second, to a maximum level (the P-level) via two intermediate steps labeled J and I (OJIPprocess). Drought factor index (DFI) was also calculated using measured OJIP parameters. The DFI was -0.22,meaning not only the initial inhibition of PSII but also sequential inhibition of PSI . In real, most of allphotochemical parameters such as quantum yield of the electron transport flux from Quinone A (QA) toQuinone B (QB), quantum yield of the electron transport flux until the PSI electron acceptors, quantum yieldof the electron transport flux until the PSI electron acceptors, average absorbed photon flux per PSII reactioncenter, and electron transport flux until PSI acceptors per cross section were profoundly reduced exceptnumber of QA reducing reaction centers (RCs) per PSII antenna chlorophyll (RC/ABS). It was illuminated thatat least 6 parameters related with quantum yield/efficiency and specific energy fluxes (per active PSII RC)could be applied to be used as the drought stress index. Furthermore, in the combination of parameters, drivingforces (DF) for photochemical activity could be deduced from the performance index (PI) for energyconservation from photons absorbed by PSII antenna until the reduction of PSI acceptors. In conclusion,photochemical responses and their related parameters can be used as physiological DFI.

Evaluating the contributions of different organic matter sources to urban river water during a storm event via optical indices and molecular composition

Lee, Mi-Hee,Lee, Yun Kyung,Derrien, Morgane,Choi, Kwangsoon,Shin, Kyung Hoon,Jang, Kyoung-Soon,Hur, Jin Elsevier 2019 Water research Vol.165 No.-

<P><B>Abstract</B></P> <P>Dissolved organic matter (DOM) in river water dynamically changes with respect to its major sources during heavy rain events. However, there has been no established tool to estimate the relative contributions of different organic sources to river water DOM. In this study, the evolution in the contributions of ten different organic matter (OM) sources to storm water DOM was explored with a selected urban river, the Geumho River in South Korea, during storm events via an end-member mixing analysis (EMMA) based on fluorescence indices and Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The OM source materials included treated effluent, road runoff, groundwater, topsoil, deep soil, leaves, reeds, riparian plants, attached algae, and suspended algae. The EMMA results provided quantitative estimates of the variations in the dominant OM sources with the progress of storms. Treated effluent was the prevailing source at the beginning period of the storm, while topsoil, leaves, riparian plants, and groundwater predominated during and after the peak period. The fluorescence indices-based evaluation was consistent with the statistical comparison of the molecular formulas derived from FT-ICR-MS conducted on the ten potential OM sources and the storm samples. The observed variations in the OM sources agreed with the typical characteristics of urban rivers in connection with anthropogenic inputs and the impact of surrounding impervious surfaces. This study demonstrates the application of intuitive and facile tools in estimating the relative impacts of OM sources in urban watersheds.</P> <P><B>Highlights</B></P> <P> <UL> <LI> It is the first study using FT-ICR-MS for OM source tracking during a storm. </LI> <LI> A quantitative evaluation is firstly performed for dynamic variations in DOM sources. </LI> <LI> Treated effluent and top soil prevailed before and after peak, respectively. </LI> <LI> Optical indices-based EMMA results were consistent with FT-ICR-MS data. </LI> <LI> The EMMA provided a reliable tool for OM source tracking for urban watersheds. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

성인의 치면세균막 내 산생성능력과 치아우식경험과의 연관성

김효정(Hyo-Jung Kim),구혜민(Hye-Min Ku),이은송(Eun-Song Lee),권호근(Ho-Keun Kwon),김백일(Baek-Il Kim) 대한치과의사협회 2017 대한치과의사협회지 Vol.55 No.6

Purpose: The aim of this study was to confirm the validity of the Cariview<SUP>TM</SUP>‚ test in adults by assessing correlations between Cariview<SUP>TM</SUP>‚ scores and caries experience using a quantitative light-induced fluorescence (QLF) technique. Methods: This retrospective cross-sectional study included 111 patients aged 19-64 years who visited a dental clinic in Seoul from 2015 to 2016, and underwent the Cariview<SUP>TM</SUP> test and had their teeth photographed with the Qraycam. Plaque was scraped with sterilized cotton swabs, and then placed in a culture medium and incubated at 37°C for 48 h. Finally, the degree of color change was evaluated, and scored on a 0-100 point scale. Caries experience index included not only cavitated lesions but also early caries that were detected on white and fluorescence images. Participants were divided into low, moderate and high caries risk groups according to the Cariview<SUP>TM</SUP> score, and caries experience was compared between risk groups using ANCOVA. Spearman correlation coefficients were calculated to analyze the associations between Cariview<SUP>TM</SUP> scores and caries indices. Result: The mean age of the participants was 40 years, and the mean Cariview<SUP>TM</SUP> score was 53.6 ± 20.7. The WDMFT and the DMFT indices significantly increased with caries risk, and this increase was significantly different between the low- and high-risk groups. The Cariview<SUP>TM</SUP> test scores were significantly correlated with the WDMFT (r = 0.28, P = 0.003) and DMFT (r = 0.29, P = 0.002) indices. Conclusion: The Cariview<SUP>TM</SUP> test could be a clinically useful and simple method for assessing caries risk in adults.

Contrast Index를 이용한 형광지문 증강 정도의 수치적 판단

전소영(Soyoung Jeon),기진영(Jinyoung Ki),김규연(Kyuyeon Kim),김지연(Jiyeon Kim),김채원(Chaewon Kim),유제설(Jeseol Yu) 한국분석과학회 2018 분석과학 Vol.31 No.5

Comparison of Clinical Characteristics of Fluorescence in Quantitative Light-Induced Fluorescence Images according to the Maturation Level of Dental Plaque

( Eun-ha Jung ),( Hye-young Oh ) 한국치위생과학회 2021 치위생과학회지 Vol.21 No.4

Background: Proper detection and management of dental plaque are essential for individual oral health. We aimed to evaluate the maturation level of dental plaque using a two-tone disclosing agent and to compare it with the fluorescence of dental plaque on the quantitative light-induced fluorescence (QLF) image to obtain primary data for the development of a new dental plaque scoring system. Methods: Twenty-eight subjects who consented to participate after understanding the purpose of the study were screened. The images of the anterior teeth were obtained using the QLF device. Subsequently, dental plaque was stained with a two-tone disclosing solution and a photograph was obtained with a digital single-lens reflex (DSLR) camera. The staining scores were assigned as follows: 0 for no staining, 1 for pink staining, and 2 for blue staining. The marked points on the DSLR images were selected for RGB color analysis. The relationship between dental plaque maturation and the red/green (R/G) ratio was evaluated using Spearman’s rank correlation. Additionally, different red fluorescence values according to dental plaque accumulation were assessed using one-way analysis of variance followed by Scheffe’s post-hoc test to identify statistically significant differences between the groups. Results: A comparison of the intensity of red fluorescence according to the maturation of the two-tone stained dental plaque confirmed that R/G ratio was higher in the QLF images with dental plaque maturation (p<0.001). Correlation analysis between the stained dental plaque and the red fluorescence intensity in the QLF image confirmed an excellent positive correlation (p<0.001). Conclusion: A new plaque scoring system can be developed based on the results of the present study. In addition, these study results may also help in dental plaque management in the clinical setting.