http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 주제어 : Succinylacetone (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
-
Myxococcus xanthus의 protoporphyrin IX의 합성과 세포 성장에 대한 succinylacetone의 영향
이병욱 한국생명과학회 2003 생명과학회지 Vol.13 No.6
Heme 합성의 중간체이며 또한 광수용체로도 작용하는 protoporphyrin IX의 세포 내 농도 및 성장 배지에 존재하는 농도가 야생형 M. xanthus DK1622 균주로부터 측정되었다. Protoporphyrin IX의 세포 내 농도는 배양 시간이 경과함에 따라 계속 증가하여, 안정기에 최고치에 이르는 것으로 나타났다 안정기에 도달한 세포 내에는 6.4 picomoles/mg of protein의 protoporphrin IX이 존재하는 것으로 밝혀졌다. Protoporphyrin IX은 대수기 중간 시기부터 세포외로 분비가 시작되어, 안정기에 도달한 세포의 배양액에서는 세포의 단백질 대비하여 3.0 picomoles/mg of protein이 존재하는 것으로 측정되었다. 영양분의 고갈에 기인하여 형성된 포자에서도 protoporphyrin IX의 농도는 6.5 picomoles/mg of protein이 존재하는 것으로 관찰되었다. Succinylacetone을 $500\muM$ 농도로 성장 배지에 첨가하였을 경우에 protoporphyrin IX의 생산은 검출이 불가능할 정도로 방해를 받았으며, 세포성장이 저해되고 세포 성장은 정상의 절반 수준인 약 100 Klett unit에서 정지하는 것으로 나타났다. 하지만 포자의 형성은 succinylacetone의 첨가에 관계없이 89-100%의 생성율을 보였음으로 정상 농도의 protoporphyrin IX가 M. xanthus의 성장을 위해서는 중요하지만, 포자 형성 과정에 필수적인 것으로 보이지는 않는다. 안정기 세포에서 나타나는 photolysis 현상도 succinylacetone의 첨가 여부에 관계없이 유사한 수준으로 관찰되었다. Protoporphyrin IX is an intermediate molecule in the heme biosynthetic pathway. Intra- and extracellular concentrations of protoporphyrin IX in the wild type strain, Myxococcus xanthus DK1622 were measured by reverse phase HPLC. The amount of intracellular protoporphyrin IX continuously increased and reached 6.4 picomoles/mg of protein at the stationary phase. Extracellular protoporphyrin IX began to be detected from the mid-exponential phase. The culture supernatant that was collected in the stationary phase contained approximately 3.0 picomoles of proto-porphyrin IX per mg of protein. Spores formed by nutrient depletion contained about 6.5 picomole protoporphyrin IX/mg of protein. The synthesis of protoporphyrin IX and cell growth were strongly inhibited by addition of succinylacetone to a final concentration of $500\muM$. Succinylacetone, however did not appear to interfere developmental processes. Normal developmental behaviors including aggregation and spore formation was achieved even if succinylacetone was added in a medium. Photolysis among cells grown on a starvation medium supplemented with succinylacetone was also observed. These results indicate that protoporphyrin IX may be important to M. ,xanthus vegetative growth, but not critical to development processes.
-
타이로신 혈증 2례; 간암이 유발된 1례와 급성 간부전으로부터 회복된 1례의 비교
김숙자,송웅주,전영미,Kim, Sook Za,Song, Woong Ju,Jeon, Young Mi,Levy, Harvey L. 대한유전성대사질환학회 2013 대한유전성대사질환학회지 Vol.13 No.1
Tyrosinemia I (fumarylacetoacetate hydrolase deficiency) is an autosomal recessive inborn error of tyrosine metabolism that produces liver failure in infancy or a more chronic course of liver disease with cirrhosis, often complicated by hepatocellular carcinoma in childhood or early adolescence. We studied a 37-year-old woman with tyrosinemia I whose severe liver disease in infancy and rickets during childhood were resolved with dietary therapy. From 14 years of age, she resumed unrestricted diet with the continued presence of the biochemical features of tyrosinemia, yet maintained normal liver function. In adult years, she accumulated only a small amount of succinylacetone. Despite this evolution to a mild biochemical and clinical phenotype, she eventually developed hepatocellular carcinoma. Her fumarylacetoacetate hydrolase genotype consists of a splice mutation, IVS6-1G>T, and a novel missense mutation, p.Q279R. Studies of resected liver revealed the absence of hydrolytic activity and immunological expression of fumarylacetoacetate hydrolase in tumour. In the non-tumoral areas, however, 53% of normal hydrolytic activity and immunologically present fumarylacetoacetate hydrolase were found. This case demonstrates the high risk of liver cancer in tyrosinemia I even in a seemingly favorable biological environment. In this study of tyrosinemia I, Case 2 with negative succinylacetone accumulation and the recovery of acute liver failure was compared with Case 1. Diet restriction and NTBC treatment are crucial to prevent hepatocellular carcinoma until liver transplant can take place and cure the condition. Further studies are needed to examine cases where liver cancer did not result despite clinical symptoms/signs of tyrosinemia type I.
-
Synthesis and Immunosuppressive Activity of Novel Succinylacetone Analogues
TeakHyeonKim,DongRyunOh,나희삼,HyunChulLee 대한약학회 2003 Archives of Pharmacal Research Vol.26 No.3
This study describes the synthesis of novel enol esters (3) and triketones (4) as analogues of succinylacetone (SA) (Ed- this abbreviation is introduced here based on your use of it in the body of the paper) and the evaluation on the mouse allogeneic mixed lymphocyte reaction (MLR) and the murine model of antigen-induced paw edema formation for immunosuppressive activity. Enol esters (3a-f) were about 2-4 fold more potent than SA in in vitro activity.
-
Synthesis and Immunosuppressive Activity of Novel Succinylacetone Analogues
Kim, Taek-Hyeon,Oh, Dong-Ryun,Na, Hee-Sam,Lee, Hyun-Chul The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.3
This study describes the synthesis of novel enol esters (3) and triketones (4) as analogues of succinylacetone (SA) (Ed- this abbreviation is introduced here based on your use of it in the body of the paper) and the evaluation on the mouse allogeneic mixed lymphocyte reaction (MLR) and the murine model of antigen-induced paw edema formation for immunosuppressive activity. Enol esters (3a-f) were about 2-4 fold more potent than SA in in vitro activity.
-
Lee Beomki,Heo Won Young,Kim Jee Ah,Lee Hyun-Seung,Hwang Narae,Park Hyung-Doo,Sung Se In,Chang Yun Sil,Park Won Soon,Lee Soo-Youn 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.2
Background: Despite the popularity of the NeoBase 2 Non-derivatized MSMS assay (PerkinElmer, Turku, Finland), there are no reports of its comprehensive evaluation, including the ability to distinguish transient tyrosinemia of the newborn (TTN) from tyrosinemia type 1 (TYR 1) using succinylacetone (SUAC). No newborn screening (NBS) cutoffs for preterm neonates in the Korean population have been suggested. We evaluated the NeoBase 2 assay and identified analytes requiring different cutoffs in preterm neonates. Methods: Residual NBS dried blood spot samples and proficiency testing (PT) materials of the Newborn Screening Quality Assurance Program and the Korean Association of External Quality Assessment Service were used. Precision, accuracy, limit of detection (LOD), lower limit of quantification (LLOQ), linearity, recovery, carryover, and performance of SUAC were evaluated. Cutoffs were determined, and analytes requiring different cutoffs in preterm neonates were investigated. Results: Mean CVs for within-run and between-day precision were within 15%. Accuracy analysis indicated high agreement with in-house derivatized assay results and results of other PT participants. All analytes demonstrated acceptable LOD, LLOQ, and linearity. Recoveries were acceptable, except for SUAC. Carryover was negligible. Cutoffs were established for all analytes; Tyr, adenosine, and C20:0-lysophosphatidylcholine required different cutoffs in preterm neonates. Differential diagnosis of TYR 1 and TTN was successful with simultaneous Tyr and SUAC measurement. Conclusions: The NeoBase 2 assay demonstrated satisfactory performance. The additional analytes provide a wider diagnostic coverage, and the simultaneous measurement of Tyr and SUAC is efficient in excluding TYR 1. The new cutoffs for preterm neonates may decrease false-positive rates, without compromising diagnostic sensitivity.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
