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- 주제어 : Nuclear speckles (검색결과 6 건)
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Kim, Chang-Hyun,Kim, Young-Dae,Choi, Eun-Kyung,Kim, Hye-Ran,Na, Bo-Ra,Im, Sin-Hyeog,Jun, Chang-Duk American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.12
<P>Nuclear speckles are subnuclear storage sites containing pre-mRNA splicing machinery. Proteins assembled in nuclear speckles are known to modulate transcription and pre-mRNA processing. We have previously identified nuclear speckle-related protein 70 (NSrp70) as a novel serine/arginine (SR)-related protein that co-localizes with classical SR proteins such as serine/arginine-rich splicing factor 1 (SRSF1 or ASF/SF2) and SRSF2 (SC35). NSrp70 mediates alternative splice site selection, targeting several pre-mRNAs, including CD44 exon v5. Here we demonstrated that NSrp70 interacts physically with two SR proteins, SRSF1 and SRSF2, and reverses their splicing activity in terms of CD44 exon v5 as exon exclusion. The NSrp70 RS-like region was subdivided into three areas. Deletion of the first arginine/serine-rich-like region (RS1) completely abrogated binding to the SR proteins and to target mRNA and also failed to induce splicing of CD44 exon v5, suggesting that RS1 is critical for NSrp70 functioning. Interestingly, RS1 deletion also resulted in the loss of NSrp70 and SR protein speckle positioning, implying a potential scaffolding role for NSrp70 in nuclear speckles. NSrp70 contains an N-terminal coiled-coil domain that is critical not only for self-oligomerization but also for splicing activity. Consistently, deletion of the coiled-coil domain resulted in indefinite formation of nuclear speckles. Collectively, these results demonstrate that NSrp70 acts as a new molecular counterpart for alternative splicing of target RNA, counteracting SRSF1 and SRSF2 splicing activity.</P>
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Kim, D.W.,Jeon, S.J.,Hwang, S.M.,Hong, J.C.,Bahk, J.D. Elsevier Scientific Publishers Ireland Ltd 2016 Plant science Vol.250 No.-
<P>Eukaryotic C3H-type zinc finger proteins (Znfs) comprise a large family of regulatory proteins involved in many aspects of plant stress response, growth and development. However, compared to mammalian, only a few plant Znfs have been functionally characterized. Here, T-DNA inserted gdsl (growth, development and splicing 1) mutant, displayed abnormal growth throughout the lifecycle owing to the reduction of cell size and number. Inverse PCR analysis revealed that the abnormal growth was caused by the disruption of At3g47120, which encodes a C3H42 protein belonging to the C-X-7-C-X-5-C-X-3-H class of the Znf family. GDS1 was ubiquitously transcribed, but shows high levels of expression in young seedling and unexpanded new leaves. In gdsl, the transcripts of many growth- and development-related genes were down-regulated, and the auxin response was dramatically reduced. A fluorescence-based assay revealed that the GDS1 protein was localized to the nucleus, prominently in the speckle compartments. Its arginine/serine dipeptide-rich-like (RS-like) domain was essential for nuclear localization. In addition, the SRI, SRm102 and U1-70K components of the U1 spliceosome interacted with GDS1 in the nuclear speckle compartments. Taken together, these suggest that GDS1, a nuclear-speckle-associated Znf, might play a significant role in splicing during plant growth and development. (C) 2016 Elsevier Ireland Ltd. All rights reserved.</P>
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간접면역형광법을 이용한 항핵항체검사에서 Dense Fine Speckled 양상의 임상적 의의
강소영,이우인 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.2
Background : Dense fine speckled (DFS) pattern in antinuclear antibody (ANA) test using indirect immunofluorescence method became to be known recently and it is detected in patients with various chronic inflammatory diseases as well as in healthy individuals. We investigated the relation between DFS pattern and various diseases. Methods : ANA tests by indirect immunofluorescence method using HEp-2 cell line slide (Kallestad; Bio-Rad, USA) were performed in 2,654 patients for screening of systemic autoimmune diseases. The frequencies of ANA and DFS positivity were analyzed according to sex, age, clinical department and disease. Results : ANA was positive in 13.3% (352/2,654) of the total patients, and the frequency of DFS pattern was observed in 3.8% (101/2,654) of the total patients and in 28.7% (101/352) of the patients with ANA positivity. Higher frequency of DFS positivity was observed in patients referred from Departments of Rheumatology and Nephrology, but there was no difference in the frequencies of DFS positivity among the patients with ANA positivity. The frequency of DFS pattern was higher in seborrheic dermatitis (14.3%), herpes zoster (11.1%), rheumatoid arthritis (16.9%), systemic lupus erythematosus (15.4%) and Sjogren syndrome (14.3%). Conclusions : The DFS pattern is a frequent finding (about 28% of ANA positivity) in ANA test using indirect immunofluorescence method. Relatively high frequency of DFS pattern was observed in autoimmune diseases, contrary to the previous observations that DFS pattern is not related with autoimmune diseases. Further studies including the confirmation tests of anti-DFS70 are needed for the identification of relation between DFS pattern and particular diseases. 배경 : 최근 간접면역형광법을 이용한 항핵항체(ANA) 검사에 서 특징적인 dense fine speckled (DFS) 양상이 각종 만성염증 성질환 외에도 정상인에서까지 발견되는 것으로 알려졌다. 저자 들은 ANA 검사가 의뢰된 환자들에서 DFS 양상을 보이는 환자 들의 빈도를 조사하고, 임상과별 및 질환별로 DFS 양상을 보인 환자들을 분석하여 특정 질환과의 연관성이 있는지 살펴보고자 하였다. 방법 : 전신자가면역질환의 선별검사 목적으로 ANA 검사가 의뢰된 2,654명의 환자를 대상으로 HEp-2 세포 슬라이드(Kallestad TM; Bio-Rad, Redmond, WA, USA)를 사용한 간접면역 형광법으로 ANA 검사를 시행하였다. ANA 양성 및 DFS 양성 빈도를 성별, 연령별, 임상과별 및 질환별로 분석하였다. 결과 : 2,654명 중 DFS 양성은 101명(3.8%)으로 나타났고 이 는 ANA 양성(352명, 13.3%)의 28.7%인 것으로 나타났다. 임상 과 간 비교에서 DFS 양성 빈도는 류마티스내과 및 신장내과에 서 높았고, ANA 양성자 중 DFS 양성 빈도의 차이는 관찰되지 않았다. 피부과의 경우 지루피부염(14.3%) 및 대상포진(11.1%) 환자에서 DFS 양성 빈도가 높았고, 류마티스내과의 경우 류마 티스관절염(16.9%), 전신홍반루푸스(15.4%) 및 쇼그렌증후군 (14.3%)에서 DFS 양성 빈도가 높았다. 결론 : DFS 양상은 간접면역형광법을 이용한 ANA 검사에서 양성 건수의 약 28%를 차지할 정도로 빈번히 관찰되는 소견으 로 나타났다. DFS 양상과 자가면역질환과의 연관성이 없다는 기존의 보고와 달리 본 연구에서 높은 양성률을 보이므로 특정 질환과의 연관성 유무를 확인하기 anti-DFS70 확인검사를 포 함하는 연구가 뒤따라야 할 것으로 판단된다.
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조재완(Jai-Wan Cho),정경민(Kyung-Min Jeong) 대한전기학회 2012 전기학회논문지 Vol.61 No.8
In this paper, the gamma ray irradiation test results of the CCD cameras are described. From the low dose-rate (2.11 Gy/h) to the high dose-rate (150 Gy/h) level, which is the same level when the hydrogen explosion was occurred in the 1~3 reactor unit of the Fukushima nuclear power plant, the monitoring performance of the cameras owing to the speckles are evaluated. The numbers of speckles, generated by gamma ray irradiation, in the image of cameras are calculated by image processing technique. And the legibility of the sensor indicator (dosimeter) owing to the numbers of the speckles is presented.
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