에이코사펜타인산이 돼지난포란의 체외 성숙에 미치는 영향

김강식,박흠대 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.3

Among fatty acid families, the polyunsaturated fatty acids were demonstrated to be mediators in various reproductive processes as precursor of steroid hormone (via cholesterol) and prostaglandins (via arachidonic acid), and in the last decade, major research was focused on the effects of omega-6 and especially omega-3 fatty acid. Eicosapentaenoic acid, the longest members of omega-3 fatty acid family, can be produced by a series of desaturation and elongation reactions from shorter member such as α-Linolenic acid. However, very few studies have provided detailed descriptions of Eicosapentaenoic acid effects and mechanisms of action in mammalian oocytes. The purpose of this study was to evaluate the effect of Eicosapentaenoic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of Eicosapentaenoic acid was added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, nuclear maturation rate, blastocysts quality, and levels of prostaglandin E2, 17β-estradiol, progesterone in the spent medium. High doses (100 mM) of Eicosapentaenoic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 mM Eicosapentaenoic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of E2/P4 also significantly increased compared with control group (p < 0.05). However, Supplementation of 100 mM Eicosapentaenoic acid showed high apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17b-estradiol/progesterone also significantly decreased compared with control group (p < 0.05). Our results indicated that supplementation with appropriate levels of Eicosapentaenoic acid beneficially affects the change of hormone synthesis for controlling oocyte maturation, leading to improved embryo quality. However, high doses of Eicosapentaenoic acid treatment results in detrimental effects.

에이코사펜타인산이 돼지난포란의 체외 성숙에 미치는 영향

김강식,박흠대 한국동물생명공학회(구 한국동물번식학회) 2019 Journal of Animal Reproduction and Biotechnology Vol.34 No.3

Among fatty acid families, the polyunsaturated fatty acids were demonstrated to be mediators in various reproductive processes as precursor of steroid hormone (via cholesterol) and prostaglandins (via arachidonic acid), and in the last decade, major research was focused on the effects of omega-6 and especially omega-3 fatty acid. Eicosapentaenoic acid, the longest members of omega-3 fatty acid family, can be produced by a series of desaturation and elongation reactions from shorter member such as α-Linolenic acid. However, very few studies have provided detailed descriptions of Eicosapentaenoic acid effects and mechanisms of action in mammalian oocytes. The purpose of this study was to evaluate the effect of Eicosapentaenoic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of Eicosapentaenoic acid was added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, nuclear maturation rate, blastocysts quality, and levels of prostaglandin E2, 17β-estradiol, progesterone in the spent medium. High doses (100 mM) of Eicosapentaenoic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 mM Eicosapentaenoic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of E2/P4 also significantly increased compared with control group (p < 0.05). However, Supplementation of 100 mM Eicosapentaenoic acid showed high apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17b-estradiol/progesterone also significantly decreased compared with control group (p < 0.05). Our results indicated that supplementation with appropriate levels of Eicosapentaenoic acid beneficially affects the change of hormone synthesis for controlling oocyte maturation, leading to improved embryo quality. However, high doses of Eicosapentaenoic acid treatment results in detrimental effects.

혈관내피세포-U937세포의 부착에 미치는 Eicosapentaenoic Acid의 효과

김은지,전재은,임현주,차성철,정의룡,조용근,조용근 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

연구배경: 역학적연구에서 어류의 섭취가 많은 집단에서 관상동맥질환의 빈도가 낮은 것이 보고되었고, 이것은 어류에 많이 함유된 불포화지방산(polyunsaturated fatty acid) 특히 eicosapentaenoic acid 때문으로 생각되어지고 있다. 본 연구에서는 동맥경화증 발생의 초기단계로 여겨지는 단핵구와 혈관내피세포 사이의 adhesion에 미치는 다불포화지방산의 영향을 in vitro system에서 관찰하고자 하였다. 방 법: 제대정맥에서 얻은 혈관내피세포를 배양하여 96 well에 옮기고 25, 50, 100, 200 ㎛ 농도의 eicosapentaenoic acid(EPA)를 6시간 동안 처리한 U937세포를 부착시켜 adhesion assay를 시행하였다. 혈관내피세포를 자극하기 위해서는 lipopolysaccharide(LPS)를 well당 100 ng씩을 투여하였다. 음성대조군으로는 LPS로 자극하지 않은 혈관내피세포에 EPA처리를 하지 않은 U937세포로 adhesion assay한 성적을 사용했고, 양성대조군으로는 LPS로 자극한 혈관내피세포에 EPA처리를 하지 않은 U937세포로 adhesion assay한 성적을 사용했다. 그리고 EPA(3.3 mM)을 5㎛ CuSO4 및 300 ㎛ asscorbic acid와 혼합하여 섭씨 37도에서 16시간 두어 산화-EPA를 만들어 같은 실험을 반복하였다. 결 과: U937세포에 25, 50, 100, 200 ㎛의 EPA로 6시간 처리하였을 때 U937세포의 생존율은 99% 이상이었다. 혈관내피세포를 LPS로 자극했을 때 adhesion assay에서 혈관내피세포에 붙는 U937세포의 수는 현저히 증가하였다. 그러나 EPA를 U937세포에 25, 50, 100, 200 ㎛의 농도로 6시간 처리 후에 lipopolysac-charide를 처리한 제대정맥 내피세포에 부착(adhesion)시켰을 때 처리한 EPA의 각 농도에 따른 부착된 U937세포의 수는 통계적으로 유의한 차이를 나타내지 못했다. EPA처리가 부착된 U937세포의 수에 영향을 미치지 못하였다. 결 론: EPA는 U937세포에 영향을 미쳐 혈관내피세포-U937 세포부착을 감소시키지는 못하였다. 따라서 EPA에 의한 혈관내피세포-단핵구 부착 억제 효과는 EPA의 단핵구에 미치는 영향에 의하지는 않는 것으로 생각되나 추후 더 많은 연구가 필요하리라 생각된다. Background : Epidemiological studies have shown correlation between low incidence of coronary heart disease and high consumption of fish products. It has been suggested that this may be due to the high content of polyunsaturated fatty acids of the n-3 fatty acid group in fish oil. In animal studies eicosapentaenoic and (EPA) inhibited attachment of monocytes to the arterial endothlium. Method : Adhesion assay was performed on the endothelial cells of the human umbilical vein with 25, 50, 100, 200 ㎛ EPA-treated U937 cells. The endothelial cells were activated with lipopoysaccharide (LPS). The adhesion assay was repeated with oxidized EPA. EPA was oxidized with CuSO4 and ascorbic acid. Result : Viability of U937 cells were not afected by concentrations up to 200 ㎛ of EPAand oxidized EPA. LPS treatment of endothelium notably increased the number of U937 cells attached to endothelial cells on the adhesion assay. However, treatment of EPA, native or oxidized, to U937 cells did not afect the number of U937 cells attached to LPS activated endothelial cells. Conclusion : EPA treatment, native or oxidized, of U937 cells did not affect U937 cell-endothlial cell adhesion. This suggests that inhibition of monocyte-endothlial cells ◎attachment by EPA is not due to the effects of EPS on monocytes.(Korean Circulatin J 1998;28(4):606-610)

조현병 치료보조제로서 오메가-3 지방산에 대한 국내외 논문분석

차현수(Hyun-su Cha) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.9

본 연구의 목적은 조현병 치료 보조제로 오메가-3 지방산을 사용하기 위하여 오메가-3 지방산이 조현병 환자와 조현병이 유도된 동물에게 미치는 효과를 파악하는 것이다. 문헌 검색은 ‘오메가-3 지방산’ ‘Omega-3 Fatty Acids’, ‘Docosahexaenoic Acids’, ‘Eicosapentaenoic Acid Ethyl Ester’, ‘조현병’, ‘schizophrenia’ 등을 검색어로 하여 한국 교육 학술 정보원, 국회 도서관, Pubmed, CINAHL 등을 통해 2001년 1월부터 2020년 12월까지 발표된 연구논문을 검색하였다. 연구자가 원본을 확인한 후, 선정한 9편의 논문을 최종 분석하였다. Scottish Intercollegiate Guideline Network의 체크리스트로 논문의 질적 평가를 실시한 결과 10점 만점에 9점인 연구가 3편, 8점인 연구가 5편, 7점인 연구가 1편 이었다. 오메가-3 지방산 투여량은 1g/일 - 4g/일 이었고, 투여 횟수는 1회/일 - 2회/일 이었고, 투여 기간은 12주, 16주, 26주, 2년 동안 또는 조현병이 재발할 때까지 이었다. 분석한 연구 결과에 의하면 오메가-3 지방산은 정신 증상, 뇌 , 혈액 등에 변화를 일으켜 조현병 증상을 감소시키는 효과가 있었다. 향후 조현병 치료보조제로 오메가-3 지방산이 이용되기를 바란다. This study identifies the effects of omega-3 fatty acids on patients with schizophrenia and animals induced by schizophrenia, in order to use omega-3 fatty acids as a supplement for treating schizophrenia. Literature searches included articles from January 2001 to December 2020, and search terms included the terms “Omega-3 fatty acids”, “Docosahexaenoic Acids”, “Eicosapentaenoic Acid Ethyl Ester”, and “Schizophenia”. After the original articles were reviewed by the researcher, nine papers selected by me were finally analyzed. The Scottish Intercollegiate Guideline Network’s checklist was applied for a qualitative evaluation of the papers. From a total of 10 points, three studies scored 9, five studies scored 8, and one study scored 7. Omega-3 fatty acid dose administered was 1g/day-4g/day for a duration of 12 weeks, 16 weeks, 26 weeks, 2 years, or until schizophrenia recurred. Our analysis revealed that administering omega-3 fatty acids reduces the symptoms of schizophrenia by altering the psychological symptoms, and the physiology of the brain and blood. We believe that omega-3 fatty acids have the potential to be used as supplements to treat schizophrenia.

Low Plasma Proportion of Omega 3-Polyunsaturated Fatty Acids Predicts Poor Outcome in Acute Non-Cardiogenic Ischemic Stroke Patients

Song, Tae-Jin,Cho, Hyun-Ji,Chang, Yoonkyung,Choi, Kyungsun,Jung, A-Reum,Youn, Minjung,Shin, Min-Jeong,Kim, Yong-Jae Korean Stroke Society 2015 Journal of stroke Vol.17 No.2

<P><B>Background and Purpose</B></P><P>Alterations in blood fatty acid (FA) composition are associated with cardiovascular diseases. We investigated whether plasma FA composition was related to stroke severity and functional outcome in acute ischemic stroke patients.</P><P><B>Methods</B></P><P>We prospectively enrolled 156 patients with first-episode cerebral infarction, within 7 days of symptom onset. The proportion of FAs was analyzed using gas chromatography, and the summation of the omega-3 polyunsaturated fatty acids (ω3-PUFA), 18:3 ω3 α-linolenic acid, 20:3 ω3 eicosatrienoic acid, 20:5 ω3 eicosapentaenoic acid (EPA), and 22:6 ω3 docosahexaenoic acid (DHA) was reported as Σω3-PUFAs. Stroke severity was assessed using the National Institutes of Health Stroke Scale (NIHSS) score on admission. Poor functional outcome was defined by modified Rankin scale (mRS) ≥3 at three months after the index stroke.</P><P><B>Results</B></P><P>Lower proportions of EPA (β=-0.751), DHA (β=-0.610), and Σω3-PUFAs (β=-0.462) were independently associated with higher NIHSS score, after adjusting for stroke subtype, hemoglobin, high density lipoprotein, high sensitivity C-reactive protein, fasting glucose, 16:0 palmitic acid, and Σsaturated fatty acids. Moreover, a lower proportion of DHA (odds ratio [OR]: 0.20, 95% confidence interval [CI]: 0.04-0.88), and Σω3-PUFAs (OR: 0.22, 95% CI: 0.05-0.84) showed an independent relationship with poor functional outcome after adjusting for age, sex, smoking status, NIHSS score, stroke subtype, and 16:0 palmitic acid.</P><P><B>Conclusions</B></P><P>Our results demonstrate that ω3-PUFAs correlated with stroke severity on admission and functional outcomes at 3 months. ω3-PUFAs are potential blood biomarkers for prognosis of acute non-cardiogenic ischemic stroke patients.</P>

혈관내피세포-U937세포의 부착에 미치는 Eicosapentaenoic Acid의 효과

채성철,김은지,임현주,정위룡,조용근,전재은,박의현 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.4

Cloning and Identification of a Novel C18-Δ9 Polyunsaturated Fatty Acid Specific Elongase Gene from DHA-producing Isochrysis galbana H29

Ming Li,Xiuyuan Ou,Xiangdong Yang,Dongquan Guo,Xueyan Qian,Laijun Xing,Mingchun Li 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1

Isochrysis galbana, a marine prymnesiophyte microalga, is able to produce a high level of long chain polyunsaturated fatty acids such as docosahexaenoic acid (DHA, C22:6n-3). In this article, a novel gene (IgASE2)that encoded a C18-Δ9 polyunsaturase fatty acids specific (C18-Δ9-PUFAs-specific) elongase was isolated and characterized from DHA-rich microalga, I. galbana H29. A full-length cDNA of 1653 bp was cloned by rapidamplification of cDNA ends (RACE) PCR techniques. The IgASE2 contained a 786 bp ORF encoding a protein of 261amino acids that shared 87% identity with the reported Δ9-elongase IgASE1, a 44 bp 5' untranslated region and an 823 bp 3' untranslated region. The function of IgASE2 was demonstrated by its heterologous expression in Saccharomyces cerevisiae. In S. cerevisiae, IgASE2 elongated linoleic acid (LA, C18:2n-6), α-linolenic (ALA, C18:3n-3) to eicosadienoic acid (EDA, C20:2n-6) and eicosatrienoic acid (ETrA, C20:3n-3). The conversion ratios of LA to EDA and ALA to ETrA were 60.47 and 58.36%, respectively. However, IgASE2 could not catalyze the elongation reactions of oleic acid (OA, C18:1n-9) and other fatty acids. These results confirmed that IgASE2 had C18-Δ9-PUFAs-specific elongase activity. Isochrysis galbana, a marine prymnesiophyte microalga, is able to produce a high level of long chain polyunsaturated fatty acids such as docosahexaenoic acid (DHA, C22:6n-3). In this article, a novel gene (IgASE2)that encoded a C18-Δ9 polyunsaturase fatty acids specific (C18-Δ9-PUFAs-specific) elongase was isolated and characterized from DHA-rich microalga, I. galbana H29. A full-length cDNA of 1653 bp was cloned by rapidamplification of cDNA ends (RACE) PCR techniques. The IgASE2 contained a 786 bp ORF encoding a protein of 261amino acids that shared 87% identity with the reported Δ9-elongase IgASE1, a 44 bp 5' untranslated region and an 823 bp 3' untranslated region. The function of IgASE2 was demonstrated by its heterologous expression in Saccharomyces cerevisiae. In S. cerevisiae, IgASE2 elongated linoleic acid (LA, C18:2n-6), α-linolenic (ALA, C18:3n-3) to eicosadienoic acid (EDA, C20:2n-6) and eicosatrienoic acid (ETrA, C20:3n-3). The conversion ratios of LA to EDA and ALA to ETrA were 60.47 and 58.36%, respectively. However, IgASE2 could not catalyze the elongation reactions of oleic acid (OA, C18:1n-9) and other fatty acids. These results confirmed that IgASE2 had C18-Δ9-PUFAs-specific elongase activity.

심혈관계질환의 위험지표로서Omega-3 Index에 대한 올바른 이해 및 한국인에의 적용

박용순 대한비만학회 2010 The Korean journal of obesity Vol.19 No.1

오메가-3 다가 불포화지방산인 eicosapentaenoic acid(EPA; C20:5)와 docosahexaenoic acid(DHA; C22:6)는 심혈관계질환으로 인한 사망을 예방하는 영양소로 잘 알려져 있다. 최근에는 오메가-3 지방산이 심혈관계질환뿐 아니라 뇌졸중을 예방하는데도 도움이 된다는 연구결과가 보고되고 있으며, 이러한 오메가-3 지방산의 효과는 항부정맥, 항염증, 혈액응고 억제, 지단백질 농도 저하 등과 관련이 있다고 한다. Omega-3 Index는 오메가-3 지방산의 체내 수준을 나타내는 유일한 표준 혈액 검사로 적혈구의 EPA와 DHA 수준을 의미한다. Omega-3 Index는 섭취한 오메가-3 지방산과 비례할 뿐 아니라, 심장근육의 오메가-3 지방산 농도와도 비례함하고, 심혈관계질환의 위험도와 음의 상관관계를 가진다고 알려져 심혈관계질환의 위험지표로 활용되고 있다. 권장섭취량인 1g의 오메가-3 지방산으로 얻을 수 있는 8-10% Omega-3 Index가 심혈관계질환의 예방을 위한 적정 목표치로 설정되었다. 한국인에게도 Omega-3 Index는 활용가능하나 목표치는 상향 조정이 필요하다고 사료된다. Of all known dietary factors, long-chain omega-3 fatty acids (eicosapentaenoic acid and docosahexaenoic acid) may be the most protective against death from cardiovascular disease. Evidence continues to accrue for benefits of omega-3 fatty acids in the prevention of coronary heart disease and stroke. Anti-arrhythmogenic, anti-inflammatory, anti-thrombotic and hypolipidemic effects of omega-3-fatty acids are emerging as the most likely explanation. New evidence has confirmed and refined the cardioprotective risk factor of Omega-3 Index, the only standardized blood test of omega-3 fatty acids measuring content of erythrocyte eicosapentaenoic and docosahexaenoic acids. Current evidence suggests that individuals with coronary artery disease may reduce their risk of sudden cardiac death by increasing their intake of long-chain omega-3 fatty acids by approximately 1 g per day and achieving a target range of 8-10% Omega-3 Index. However, target range of Omega-3 Index for Koreans may be higher than 10%.

Effects of α-Linolenic, Eicosapentaenoic and Docosahexaenoic Acids on the Content and Fatty Acid Composition of Brain Phospholipid in Rats

Cha, Jae-Young,Cho, Young-Su 한국응용생명화학회 1999 Journal of Applied Biological Chemistry (J. Appl. Vol.42 No.2

The effects of dietary n-3 fatty acids, ${\alpha}$-linolenic acid (18:3), eicosapentaenoic acid (EPA, 20:5), and docosahexaenoic acid (DHA, 22:6), on brain phospholipid content and fatty acid composition were compared in rats fed with a diet containing constant ratios of saturated fatty acid/monounsaturated fatty acid/polyunsaturated fatty acid (PUFA) and n-3/n-6. The dietary fat in each diet was added at the level of 10%. In each diet, n-3 PUFA comprised two-thirds of the PUFA and the remaining one-third was linoleic acid (18:2). Dietary fat containing linoleic acid as the sole source of PUFA was also given to the control group. The content of brain phospholipid in the three n-3 PUFA groups was significantly lower than that of the linoleic acid group. This reduction was greater in the EPA and DHA groups than in the ${\alpha}$-linolenic acid group. The decrease in phospholipid content in rats fed n-3 fatty acid-rich diets was largely due to the decrease in the phosphatidylethanolamine fraction. Each dietary n-3 PUFA was found to affect the fatty acid composition of brain phospholipids; the most pronounced alteration was observed in phosphatidylethanolamine fraction. Furthermore, the proportion of DHA in the phosphatidylethanolamine fraction tended to be higher in the DHA group than in other PUFA groups. In conclusion, dietary ${\alpha}$-linolenic acid, EPA and DHA can influence the phospholipid content, phospholipid subclass, and fatty acid composition in rat brain.

사람과 쥐에서 식이내 n6 와 n3 불포화지방산이 혈장 지질조성에 미치는 영향

박현서,남정혜,최주선 慶熙大學校 1991 論文集 Vol.20 No.-

To compare the plasma lipid-lowering effect of dietary n6 and n3 polyunsaturated fatty acids(PUFA) in human and rats, ten college women were divided into 5 groups and treated for 5 weeks with 1 week interval between treatments and subjects serving as their own controls. Male Sprague Dawley rats were divided into 3 groups and fed the experimental diets for 6 weeks. The experimental diets were corn oil diet for n6 linoleic acid, perilla oil diet for n3 α-linolenic acid and fish oil diet for n3 eicosapentaenoic acid (EPA) and docosahexaenoic acid(DHA). Dietary fat was supplied at 30% Cal and modified to give the total amount of satu-rated fatty acid(SFA) and monoenoic acid(MEA)at constant level. In human, there was no significant effect on plasma cholesterol (Chol)level by different PUFA. However, on a gram­for­gram basis, there was a trend that the decrease in plasma Chol was proportionate to the degree of fat unsaturation. HDL―Chol content was significantly increased only in fish oil diet but no change in the relative proportion of its chemical components of HDL. In rat, plasma Chol and HDL―Chol levels were significantly lowered by fish oil than corn oil and perilla oil. Plasma Chol―lowering effect in human and rats was greater by n3 EPA and DHA than n6 linoleic acid. Plasma triglyceride(TG)level in human was significantly lowered only in fish oil diet but no significant effect on the relative proportion of TG in VLDL. Plasma TG in rats was significantly decreased by both perilla oil and fish oil than by corn oil. Therefore, plasma TG―lowering effect was in the order of n3 EPA+DHA>n3 α­linolenic acid >n6 linoleic acid. In conclusion, perilla oil and fish oil which contain n3 series PUFA may have important nutritional benefit in the prevention and treatment of atherosclerotic disease.