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차성철,김대성,최훈,손상욱,배현덕 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.2
디지털 워터마크는 디지털 자료를 모사로부터 보호하는 방법이다. 그르므로 디지털 워터마크는 파일변환, 컬러변환, 약간의 몽롱화, 압축과 같은 치리에서 뿐 아니라 인쇄, 복사, 스캐닝에 대해서도 강인해야한다. 본 논문은 워터마크 추출을 위한 키 데이터와 독립성분분석을 이용하는 알고리즘을 제안한다. 이 기법에서는 워터마크는 사람의 눈에 덜 민감한 원 영상의 웨이블릿변환 계수에 삽입된다. 그리고 독립성분분석 알고리즘은 서로 확률 분포가 다른 워터마크와 계수를 분리한다. 실험결과 워터마크가 워터마크 영상에서 사람 눈으로 인지할 수 없음을 보였으며, 워터마크 영상으로부터 키 데이터를 이용 영상이 일부 제거된 경우, 잡음이 첨가된 경우, JPEG 압축에서 워터마크의 추출이 가능함을 보였다. A digital watermark is a method to protect digital material from counterfeit. Therefore, it must be robust to withstand any processing, such as file format conversions, color conversions, slight blurring, sharpening color adjustment and compression as well as printing, copying and scanning. This paper proposed a method using key data and ICA algorithm for extraction of watermark. In this Method, we embed the watermark to the wavelet transform coefficients of original image which located in less sensitive subband to human eyes. ICA algorithm separated between watermark and coefficient that differs with probability distribution function each other. The experiment results have shown that the watermark is invisible to human eyes in watermarked image, and it is possible for watermarked image to extract the watermark with key data in various environment, such as removing, mixed noises and JPEG compressions.
중간등급의 기관지 점액표피양 암종 와 국내 보고에 대한 고찰
차성철 ( Sung Chul Cha ),김시우 ( Si Woo Kim ),조유진 ( Yoo Jin Cho ),박성균 ( Sung Kyoon Park ),박현근 ( Hyun Keun Park ),김종상 ( Jong Sang Kim ),곽재욱 ( Jae Wook Kwak ),유문빈 ( Moon Bin Yoo ),조혜제 ( Hye Jae Cho ),이재진 ( J 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.65 No.1
Bronchial mucoepidermoid carcinoma is uncommon, representing 0.2% of all lung tumors. The disease usually presents with symptoms of airway obstruction and recurrent pneumonia. It is commonly classified into two grades in Korea, low and high. We report a case of a bronchial mucoepidermoid carcinoma in a 40-year-old woman who complained of symptoms of an upper respiratory infection. The histological grade after a bronchoscopic biopsy was intermediate. A left upper lobectomy was performed as treatment. The TNM stage of this case was IA (T1N0M0). In addition, 25 cases of bronchial mucoepidermoid carcinoma from 1984 in Korea are also reviewed from the viewpoint of the relationship between the histological grade, TNM stage and clinical course of the tumor.
Studies on the Coating Materials for Forming Dies of Advanced High Strength Steel Sheets
Sungchul Cha(차성철),Hoyoung Kong(공호영),Iksoo Kim(김익수),Kunuk Park(박건욱),Hyundal Park(박현달) 한국자동차공학회 2012 한국자동차공학회 부문종합 학술대회 Vol.2012 No.5
To increase the safety and lighten the weight of car body and chassis, the application of advanced high strength steel (AHSS) is being rapidly expanded. Current forming dies with thermal diffusion (TD) of vanadium carbide or coatings (AlTiCrN+MoS2, TiAlN) don’t have enough wear resistance and durability. The coatings shall have complex material properties, e.g. wear, temperature, fatigue resistance and low friction property. The main concept of coating and layer development is to add the low friction property through carbon addition to temperature resistant elements. The toughness, fatigue resistance and bonding are realized through nitrided layer, CrN or TiN and multilayer structure. Total 18 coatings are compared and discussed in this work. As conclusion, TiAlCN and AlTiCrN+CN show the competitive solutions.
클로닝된 Bacillus thuringiensis subsp. kurstaki HDI 살충성 단백질 유전자의 대장균에서의 발현
황성희,차성철,유관희,이형환 한국미생물 · 생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.6
Bacillus thurintensis subsp. kurstaki HD1 살충성 단백질 ICP 유전자가 있는 NdeI 단편 3.856 kb를 클로닝하여 제조한 pHLN2-80(-) 클론이 pHLN1-80(-)에 비해서 대장균에서 ICP발현량이 과다발현되는 현상을 규명하고자 하였다. 본 연구에서는 상기의 pHLN2-80(+) 클론의 발현량을 조절하는 원인을 규명하기 위하여 ICP의 아미노산 서열은 변화되지 않는 범위 내에서 pHLN1-80(+) 클론에 있는 Plac프로모터와 ICP유전자 프로모터의 일부인 -80 bp프로모터의 염기서열, 전사 개시점과 종결부위의 변이가 ICP유전자발현에 미치는 영향을 조사하였다. pHLN1-80(+)에 5'-말단에 존재하는 -80 bp 프로모터만을 보유한 pHLNK-80 클론은 ICP 생산은 매우 저조하였다. Plac프로모터와 -80 bp 프로모터의 구조 골격을 일부 변이 시킨 pHLNF1-80클론의 ICP생산량은 pHLN2-80(-)가 생산한 양보다는 낮아서 과다발현이 안되었다. Plac프로모터 상류를 약 350bp을 제거하여 만든 클론 pHLND2-80의 ICP 생산량은 모클론인 pHLN2-80(-) 보다 매우 높게 과다발현 되었다. ICP 유전자의 과다발현 현상에 대한 전사 개시점과 전사종결 부위의 역할을 알아보기 위해서 -72bp ICP유전자프로모터를 갖는 클론 pHLD1-72는 재조합 클론 pHLN2-80(-)가 생산한 양보다 적은 양의 ICP을 생성하였고, 클론 pHLD2-72는 재조합 클론 pHLN2-80(-)보다 적은 ICP을 발현하여 과다 발현되었으며, 클론 pHLN2-72는 모클론인 pHLN2-80(-)보다 약간 높은 ICP 생산량을 보여 과다발현되었다. 클론 pHLN2-72를증식하여 파쇄액을 만든 후에 Bombyx mori유충에 대한 살충력 검사에서 클론 pHLN2-72이 생산한 ICP는 pHLN1-80(+)이 생산한 ICP보다 약 90배의 살충력을 보였다. SDS-PAGE와 Western blot 분석에서도 클론 pHLN2-72는 재조합 클론 pHLN2-80(-)보다 약간 높게 ICP가 생성이 되었었다. 이상의 결과는 과다발현에 Plac프로모터와 종결부위가 반드시 필요하며, -72 bp ICP 프로모터가 -80 bp 프로모터보다 과다발현률이 높았으며, ICP 유전자는 반드시 Plac프로모터의 전사 방향에 역방향으로 삽입이 되어야 하는 것으로 나타났다. The expression in Escherichia coli of a cloned insecticidal protein (ICP) gene from Bacillus thuringiensis var. kurstaki HD1 in pHLN1-80 (+) and pHLN2-80(-) plasmids was investigated through deletions in promoters, transcription start point, and termination region. Six recombinant plasmids were constructed in an attempt to analyze the overexpression of the ICP in relations to its gene structure. The amounts of ICP produced from the recombinants were measured by SDS-PAGE and confirmed by Western blot analysis. One clone was not overexpressed which having only -80 bp (contained BtI promoter) part of the ICP gene promoter (without Plac promoter), the right-oriented ICP gene and the termination region. Removal of 350 bp from upstream region of the Plac of the clone pHLN2-80 (-) resulted in overexpression of the ICP. One clone was not overexpressed in which the clone consisted of -72 bp part of the ICP promoter without the transcription start point and the transcriptional termination region, and having the right-oriented ICP gene sequence. One clone consisting of the inverted ICP gene sequence, the -72 bp ICP gene promoter, and without the termination region caused overexpression. One clone which consisted of the inverted ICP gene, the -72 bp ICP gene promoter and the termination sequence was overexpressed. These results indicated that the Plac promoter, transcription termination region, the inverted ICP gene insertion, and the -80 bp or -72 bp part of the ICP gene promoters were concerned in the overexpression of the ICP gene in the recombinant plasmid, and also the overexpression mechanism might result from the disruption of the transcription-suppressing regions in the promoter regions.
이형환,차성철,어홍선,조재경,이준근,장동준,김수영,Lee, Hyung-Hoan,Cha, Soung-Chul,Uh, Hong-Sun,Cho, Jae-Kyung,Lee, Jun-Keun,Chang, Dong-Jun,Kim, Soo-Young The Korean Society of Virology 1999 Journal of Bacteriology and Virology Vol.29 No.4
Zosteriform lesions, occurring after left flank and intravaginal inoculations of Balb/c mice with the Herpes simplex virus type 1 strain McKrae, developed in clinically normal skin via nerve endings. The developments of zosteriforms were standardized in 5 phases with the following references; formation of small vesicles (phase 1); occurrence of erosion and ulceration of local lesions (phase 2); occurrence of ulcerations (phase 3); occurrence of severe ulcerations (phase 4); and death (phase 5). These results provide two valuable zosteriform models to further investigate and analyze the pathological symptoms in susceptible animals infected with HSV-1 or HSV-2 and DNA vaccines.
박선아,차성철,장재혁,이형환,Park, Sun-A,Cha, Sung-Chul,Chang, Jae-Hyeok,Lee, Hyung-Hoan 대한미생물학회 1996 Journal of Bacteriology and Virology Vol.26 No.1
Hyphantria cunea nuclear polyhedrosis virus p10유전자와 프로모터의 염기서열을 결정하였고, p10단백질의 아미노산 서열을 유도했다. pBP10재조합클론 (Cha et. al., 1991)에 삽입이 되어있는 p10유전자의 염기서열을 결정한 결과 p10유전자의 ORF는 285 bp였고, p10단백질은 95개의 아미노산으로 구성 되었으며, 분자량은 10.26 kDa이었다. 프로모터내에는 TATA box와 전사개시부위인 TAAG 염기가 발견되었다. poly (A) signal부위인 AATAAA염기서열은 3'-말단상류의 65염기부위에 위치했다. p10단백질의 N-말단은 소수성이었으며, C-말단은 고도로 친수성이었다. p10단백질에는 cysteine, histidine, tryptophane, tyrosine, glutamine, asparagine잔기가 없었다. The sequences of p10 gene its promoter of Hyphantria cunea NPV were determined. According to the sequence analysis, the putative p10 gene ORF has 285 bp. The 5'-non-coding leader sequence of the p10 gene promoter contained the TATA box and the putative transcription initiation site TAAG motif. Poly (A) tail signals, AATAAA sequence was at site 65 base upstream from the 3' terminus. The deduced amino acid sequence of p10 protein was 95 with a predicted molecular weight of 10.26 kDa. In the p10 protein sequence, a hydrophobic region was present at the N-terminus of the protein, whereas the C-terminus was highly hydrophilic. The p10 protein of H. cunea NPV did not contain cysteine, histidine, trytophan, tryptophane, tyrosine, glutamine and asparagine residues.