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박혜원(Hye-Won Park),이승철(Seung-Cheol Lee) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.10
진피(진귤 과피)에 상업용 효소 Celluclast, Pectinex, Viscozyme을 처리하여 추출 수율, 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH 라디칼 소거 활성 및 아질산염 소거 활성, ACE 저해 활성, 알코올 분해대사 효소 활성을 분석하였다. 그 결과 가장 효과적으로 활성을 나타낸 효소는 Celluclast였으며, 진피와 Celluclast간의 최적 조건은 pH 4.5, 온도 50°C, 처리시간 48 h였다. Celluclast를 활용한 효소 처리는 진피의 생리활성을 효과적으로 증가시켰으며, 특히 ACE 저해 활성에 탁월한 효과를 보였다. 이상의 결과는 효소 처리 방법이 진피를 활용한 기능성 식품 개발에 도움을 줄 수 있을 것임을 시사한다. Commercial enzymes namely Celluclast, Pectinex, and Viscozyme were treated with Citrus sunki Hort. Tanaka peel (CP), and the extraction yield, total phenolic content, total flavonoid content, DPPH radical scavenging activity, nitrite scavenging activity, angiotensin converting enzyme (ACE) inhibition activity, and the effect on the alcohol metabolic enzyme activity of the CP reactants were determined. As a result, Celluclast was selected as an appropriate enzyme to increase the physiological activities of CP. The optimal reaction conditions were found to be a pH of 4.5, at 50°C for 48 h. The above results suggest that the enzyme treatment method can help in the development of functional foods using CP.
효소 처리와 유산균 배양에 의한 흑마늘의 항산화 활성 향상
체희정(Hee Jeong Chae),박동일(Dong-Il Park),이성철(Sung-Chul Lee),오철환(Chul-Hwan Oh),오남순(Nam-Soon Oh),김동청(Dong Chung Kim),원선임(Sun Im Won),인만진(Man-Jin In) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.5
마늘에 가장 많이 함유된 당류를 이용하여 유산균을 배양하기 위하여 흑마늘을 분해하여 당과 폴리페놀 함량을 최대로 증가시키는 상업용 탄수화물 분해효소로 Celluclast를 선별하였다. 또한 김치로부터 흑마늘 효소분해물에서 생육과산 생성이 우수한 유산균을 선발하였다. 선발된 균주는 Lactobacillus pentosus로 동정되었으며, L. pentosus 310-7로 명명하였다. L. pentosus 310-7 균주를 흑마늘 효소분해물에 접종한 후 pH, 적정산도, 젖산균수의 변화를 경시적으로 분석하였다. 배양액의 pH는 배양 15시간까지 pH 4.24로 지속적으로 감소하였으며, 적정산도는 배양 15시간에 0.35%까지 지속적으로 증가하였다. 젖산균수는 접종 후 7.08 log CFU/g에서 15시간에 7.54 log CFU/g까지 완만하게 증가하였으며 효소처리가 유산균의 증식에는 효과가 없었다. 그러나 효소처리 후 L. pentosus 310-7 균주로 15시간 발효시킨 발효액의 총 폴리페놀 함량은 7.23 ㎍/g에서 11.47 ㎍/g으로 약 60% 증가하였으며 따라서 DPPH radical 소거활성도 향상되었다. We investigated the improvement of the antioxidative activity of black garlic with enzymatic treatment and lactic acid bacteria cultivation conditions. Celluclast, a commercially-available polysaccharide hydrolyase, was selected to obtain high total polyphenol content in a black garlic suspension. A lactic acid bacterial strain showing fast growth and high acid production in a black garlic suspension was isolated from Kimchi. This strain was identified as Lactobacillus pentosus 310-7. Enzymatically hydrolyzed black garlic was fermented using the L. pentosus 310-7 strain at 30℃ for 15 hr. The pH and titratable acidity achieved were 4.24 and 0.35%, respectively, after 15 hr fermentation. The viable cell population of L. pentosus 310-7 slowly increased to 7.54 log CFU/g. The polyphenolic compound content, known antioxidants, in black garlic was enhanced with Celluclast treatment and L. pentosus 310-7 cultivation. Total polyphenolic compounds were increased to approximately 60% of the initial concentration, and electron donating ability was also improved, from 39.8 to 65.9%.
이승홍,박미화,한지숙,정윤화,김미숙,전유진 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.4
This study was designed to investigate whether the brown alga gamtae (Ecklonia cava) may inhibit α-glucosidase and α-amylase activities, and alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice. For that purpose, we prepared an enzymatic hydrolysate from gamtae (EHG) by using the carbohydrase, Celluclast. EHG evidenced prominent inhibitory effect against α-glucosidase and α-amylase. The IC50 values of EHG against α-glucosidase and α-amylase were 0.62 and 0.59mg/mL, respectively, which evidenced the higher activities than that of acarbose. EHG did not exert any cytotoxic effect in human umbilical vein endothelial cells (HUVECs)at various concentrations (from 0.25 to 2 mg/mL). The increase of postprandial blood glucose levels were significantly suppressed in the EHG administered group than those in the streptozotocin-induced diabetic or normal mice. Moreover, the area under curve (AUC) was significantly reduced via EHG administration (6,102 vs. 10,425 mg·min/dL) in the diabetic mice as well as it delays absorption of dietary carbohydrates. These result indicated that EHG might be a potent inhibitor for α-glucosidase and α-amylase.
남아프리카공화국 갈조류 Ecklonia maxima 줄기의 항산화 활성평가
최예지(Ye-Ji Choi),김현수(Hyun-Soo Kim),제준건(Jun-Geon Je),Lei Wang,전유진(You-Jin Jeon) 한국해양바이오학회 2019 한국해양바이오학회지 Vol.11 No.1
In the present study, the alcoholic and enzymatic extracts of Ecklonia maxima were prepared and their antioxidant activities were evaluated via the free radical scavenging activities. The lyophilized seaweed powder was extracted by 70% ethanol and the resultant extract was obtained, named as the alcoholic extract of E. maxima stem (EMSE) obtained. The residue after EMSE preparation was continuously by Celluclast-assistant extraction and then the obtained enzymatic extract was identified as polysaccharides-rich extract of E. maxima stem (EMSP). The results indicated that the extraction yields of EMSE and EMSP were 2.18% and 5.43%, respectively. In addition, the free radical scavenging activities of EMSE and EMSP were determined using an electron spin resonance (ESR) spectrometer. EMSE containing 5.36% of polyphenol and 1.56% of polysaccharide scavenged DPPH and alkyl radicals at the IC<SUB>50</SUB> values of 0.48 and 0.63 mg/mL, respectively. EMSP containing 0.33% of polyphenol and 41.58% of polysaccharide showed at the IC<SUB>50</SUB> values of 0.33 and 0.55 mg/mL, respectively. These results demonstrate that EMSP containing a high amount of polysaccharides possesses a strong free radical scavenging activity which might be a potential ingredient in pharmacological and cosmeceutical industries.
I.P. Shanura Fernando,K. K. Asanka Sanjeewa,Kalpa W. Samarakoon,P. Ranasinghe,이원우,김현수,U. K. D. S. S. Gunasekara,전유진 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.6
Enzyme-assisted extraction is a cost-effective, safe, and efficient method to obtain bioactives from plant materials. During this study, 10 different marine algae from Sri Lanka were individually extracted by using five commercial food-grade carbohydrases. The enzymatic and water extracts of the seaweeds were analyzed for their antioxidant and anti-inflammatory activities. The highest DPPH, hydrogen peroxide (H2O2) and intracellular H2O2 scavenging abilities were observed from the Celluclast extract of Sargassum polycystum (CSp). CSp exerted protective effects against oxidative stress-induced cell death in hydrogen peroxide-induced Chang cells and in model zebrafish. The Celluclast extract of Chnoospora minima (CCm) showed the strongest anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages (IC50 = 44.47 lg/mL) and in model zebrafish. CCm inhibited the levels of iNOS, COX- 2, PGE2, and TNF-a in LPS stimulated RAW 264.7 macrophages. Hence, CSp and CCm could be utilized in developing functional ingredients for foods, and cosmeceuticals.