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        Discrete Element Simulation Study on Particle Segregation Effect of a Hemispherical Shell Swing-Oscillating Trough Under Combination Swing-Oscillating

        Lingling Li,Zhenghai Liu,Jiaye Xu,Xuedong Ma 한국전기전자재료학회 2020 Transactions on Electrical and Electronic Material Vol.21 No.3

        In order to enhance the segregation eff ect of material particles in a hemispherical shell swing-oscillating trough under combination swing-oscillating, by means of three dimensional discrete element method, and by use of the two-dimensional dry granules of plastic ball and steel ball, the discrete element simulation study of particle segregation process is carried out in turn in three cases, which is composed of a single swing-oscillating in smooth trough, a combination swing-oscillating in smooth trough, and a combination swing-oscillating in adding bulge trough. The particle segregation eff ect was evaluated by particle volume concentration and combined with segregation cloud picture simulated. The result indicates that segregation effect of combination swing-oscillating is better than that in single swing-oscillating; layering eff ect of the trough added bulge is better than that in the smooth trough. And compared to single swing-oscillating in smooth trough, the degree of segregation of particles in added bulge trough can be increased by 10–15% when being combination swing-oscillating.

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        Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China

        Qian Zhang,Xindi Mei,Cheng Zhang,Juan Li,Nana Chang,Dilihuma Aji,Weifeng Shi,Yuhai Bi,Zhenghai Ma 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4

        Background: The H5 avian influenza viruses (AIVs) of clade 2.3.4.4 circulate in wild and domestic birds worldwide. In 2017, nine strains of H5N6 AIVs were isolated from aquatic poultry in Xinjiang, Northwest China. Objectives: This study aimed to analyze the origin, reassortment, and mutations of the AIV isolates. Methods: AIVs were isolated from oropharyngeal and cloacal swabs of poultry. Identification was accomplished by inoculating isolates into embryonated chicken eggs and performing hemagglutination tests and reverse transcription polymerase chain reaction (RT-PCR). The viral genomes were amplified with RT-PCR and then sequenced. The sequence alignment, phylogenetic, and molecular characteristic analyses were performed by using bioinformatic software. Results: Nine isolates originated from the same ancestor. The viral HA gene belonged to clade 2.3.4.4B, while the NA gene had a close phylogenetic relationship with the 2.3.4.4C H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated from shoveler ducks in Ningxia in 2015. The NP gene was grouped into an independent subcluster within the 2.3.4.4B H5N8 AIVs, and the remaining six genes all had close phylogenetic relationships with the 2.3.4.4B H5N8 HPAIVs isolated from the wild birds in China, Egypt, Uganda, Cameroon, and India in 2016–2017, Multiple basic amino acid residues associated with HPAIVs were located adjacent to the cleavage site of the HA protein. The nine isolates comprised reassortant 2.3.4.4B HPAIVs originating from 2.3.4.4B H5N8 and 2.3.4.4C H5N6 viruses in wild birds. Conclusions: These results suggest that the Northern Tianshan Mountain wetlands in Xinjiang may have a key role in AIVs disseminating from Central China to the Eurasian continent and East African.

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