殺戮與救贖的兩難抉擇 -余華和金英夏的父權意識比較分析

정동매 ( Dong-mei Zheng ),김학철 ( He-zhe Jin ) 중국어문연구회 2015 中國語文論叢 Vol.0 No.72

There are two similar features about Jin Yingxia, a writer from South Korea and Yu Hua from China. Both of them explore the thing of death in aesthetic style, and they also get the critical consciousness to fathers. This paper takes their novels as sample to study the features and differences of patricide consciousness in these works, to analyze the patricide impulsion and perplexity of these young writes who grew in cultural atmosphere of patriarchal system and matured in industrialized society of great change, and then reveals their introspection and seeking on social order. The two writers both grew in East Asian society with background of traditional Confucian culture, but because of different social system and culture, there are profound differences in their understanding on fathers. Jin Yingxia denied the capitalistic New Order based on interest relationship, but he still hovered in imaginative reconstruction of ideal society, while Yu Hua focused on history by revealing tininess of individual. The figure of fathers are ridiculed and deleted in Jin’s novels, while investigated and blamed in Yu’s work. As contemporary writers, they both took themselves in a contradiction of patricide impulsion and fear of lost their fathers. That is, when they attempted the new possibility of patricide consciousness, it’s hard to hide their restlessness and anxiety, and then showed their intention to do redemption for their fathers.

프레탈^(R)정(실로스타졸 50mg)에 대한 실로졸^(R)정의 생물학적 동등성

최한곤,권기철,이승호,김학미,박병주,유봉규,이종달,이경희,하정희,우종수,박인숙,최진석,용철순 한국병원약사회 2003 병원약사회지 Vol.20 No.1

Bioequivalence of two cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and the Cilozol^(R)(Hanmi Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA, Sixteen normal male volunteers(age 20~29 years old) were divided into two groups and a randomized 22 cross-over study was employed. After two tablets containing 50㎎ of cilostazol were orally administered. blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters. The results showed that the differences in C_(max), T_(max) and AUC_(t) between two tablets were 4.99%, 1.74% and 7.68%, respectively. The powers(1-β) for C_(max), T_(max) and AUC_(t) were83.92%, 80.12% and 85.03%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilozol^(R) tablet is bioequivalent to Pletaal^(R) tablet.

Synergistic Effects of Leflunomide and Benazepril in Streptozotocin-Induced Diabetic Nephropathy

Jin, Hua,Piao, Shang Guo,Jin, Ji Zhe,Jin, Ying Shun,Cui, Zhen Hua,Jin, Hai Feng,Zheng, Hai Lan,Li, Jin Ji,Jiang, Yu Ji,Yang, Chul Woo,Li, Can S.Karger 2014 The Nephron Journals Vol.126 No.3

<P>Abstract</P><P><B><I>Background:</I></B> Leflunomide (LEF) and benazepril have renoprotective effects on diabetic nephropathy (DN) through their anti-inflammatory and anti-fibrotic activities. This study investigated whether combined treatment using LEF and benazepril affords superior protection compared with the respective monotherapies. <B><I>Methods:</I></B> Diabetes was induced with streptozotocin (STZ, 65 mg/kg) by intraperitoneal injection in male Wistar rats. Two weeks after STZ injection, diabetic rats were treated daily for 12 weeks with LEF (10 mg/kg), benazepril (10 mg/kg), or a combination of both. Basic parameters (body weight, fasting blood glucose level, and 24 h urinary protein excretion), histopathology, inflammatory [inflammatory cell infiltration (ED-1), monocyte chemoattractant protein-1 (MCP-1), and Toll-like receptor-2 (TLR-2)] and glomerulosclerotic factors [transforming growth factor-β<SUB>1</SUB> (TGF-β<SUB>1</SUB>) and connective tissue growth factor (CTGF)], and oxidative stress (8-hydroxy-2'-deoxyguanosine, 8-OHdG) were studied. <B><I>Results:</I></B> Benazepril or LEF treatment significantly prevented body weight loss and 24 h urinary protein excretion induced by diabetes; combined treatment with LEF and benazepril further improved these parameters compared with giving each drug alone (all p < 0.01). Increased expression of inflammatory (MCP-1 and TLR-2) and glomerulosclerotic (TGF-β<SUB>1</SUB> and CTGF) factors in diabetic rat kidney was reduced by treatment with either LEF or benazepril and was further reduced by the combined administration of the two drugs (p < 0.01). These effects were accompanied by suppression of urinary 8-OHdG excretion. There was no significant between-group difference in blood glucose level. <B><I>Conclusions:</I></B> LEF treatment lessens DN, and combined treatment with LEF and benazepril provides synergistic effects in preventing DN.</P><P>© 2014 S. Karger AG, Basel</P>

L-carnitine treatment attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction

( Hai Yan Zhao ),( Hui Ying Li ),( Jian Jin ),( Ji Zhe Jin ),( Long Ye Zhang ),( Mei Ying Xuan ),( Xue Mei Jin ),( Yu Ji Jiang ),( Hai Lan Zheng ),( Ying Shun Jin ),( Yong Jie Jin ),( Bum Soon Choi ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.0

Background/Aims: Accumulating evidence indicates that L-carnitine (LC) protects against multiorgan damage through its antioxidant properties and preservation of the mitochondria. Little information is available about the effects of LC on renal fibrosis. This study examined whether LC treatment would provide renoprotection in a rat model of unilateral ureteral obstruction (UUO) and in vitro. Methods: Sprague-Dawley rats that underwent UUO were treated daily with LC for 7 or 14 days. The influence of LC on renal injury caused by UUO was evaluated by histopathology, and analysis of gene expression, oxidative stress, mitochondrial function, programmed cell death, and phosphatidylinositol 3-kinase (PI3K)/ AKT/forkhead box protein O 1a (FoxO1a) signaling. In addition, H₂O₂-exposed human kidney cells (HK-2) were treated with LC. Results: LC treatment inhibited expression of proinflammatory and profibrotic cytokines, and was followed by a significant attenuation of tubulointerstitial inflammation and fibrosis. The increased oxidative stress caused by UUO was associated with mitochondrial dysfunction and excessive apoptosis and autophagy via PI3K/AKT/FoxO1a-dependent signaling, and this was abrogated by administration of LC. In H₂O₂-exposed HK-2 cells, LC decreased intracellular production of reactive oxygen species, and suppressed expression of profibrotic cytokines and reduced the number of apoptotic cells. Conclusions: LC protects against the progression of tubulointerstitial fibrosis in an obstructed kidney.

Inhibition of DNA repair protein RAD51 affects porcine preimplantation embryo development

Jin, Zhe-Long,Shen, Xing-Hui,Shuang, Liang,Kwon, Jeong-woo,Seong, Min-Jeong,Kim, Nam-Hyung BioScientifica 2019 Reproduction Vol.157 No.3

<P>Homologous recombination (HR) plays a critical role in facilitating replication fork progression when the polymerase complex encounters a blocking DNA lesion, and it also serves as the primary mechanism for error-free DNA repair of double-stranded breaks. DNA repair protein RAD51 homolog 1 (RAD51) plays a central role in HR. However, the role of RAD51 during porcine early embryo development is unknown. In the present study, we examined whether RAD51 is involved in the regulation of early embryonic development of porcine parthenotes. We found that inhibition of RAD51 delayed cleavage and ceased development before the blastocyst stage. Disrupting RAD51 activity with RNAi or an inhibitor induces sustained DNA damage, as demonstrated by the formation of distinct γH2AX foci in nuclei of four-cell embryos. Inhibiting RAD51 triggers a DNA damage checkpoint by activating the ataxia telangiectasia mutated (ATM)-p53-p21 pathway. Furthermore, RAD51 inhibition caused apoptosis, reactive oxygen species accumulation, abnormal mitochondrial distribution and decreased pluripotent gene expression in blastocysts. Thus, our results indicate that RAD51 is required for proper porcine parthenogenetic activation (PA) embryo development.</P>

Rad51 Maintains Chromosome Integrity and Mitochondria Distribution during Porcine Oocyte Maturing In Vitro

Zhe-Long Jin,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2

DNA repair protein Rad51 homolog 1 (Rad51) plays a central role in Homologous recombination (HR) repair. HR depends on the formation of a Rad51 recombinase filament that facilitates strand invasion. However, whether Rad51 influences the porcine oocytes during maturation is unknown. The objective of this study is to investigate the expression and function of Rad51 during porcine oocytes maturing in vitro. Rad51 was mainly localized at the nuclear in the germinal vesicle (GV) stage and widely distributed in the cytoplasm form GV breakdown (GVBD) to MII stage. DNA damage inducing by the Etoposide was identified by the Rad51 foci formation which was localized with γH2AX. Inhibition of Rad51 increased the DNA damage, and induced metaphase I arrest with spindle defects, chromosomal misalignments and abnormal spindle assembly checkpoint (SAC) activity. Inhibition of Rad51 also increase ROS level and induced the abnormal mitochondrial distribution. Our results indicate that Rad51 play a critical role to maintain chromosome integrity as well as mitochondria activity during the porcine oocyte maturation.

Biometric cryptosystems: A new biometric key binding and its implementation for fingerprint minutiae-based representation

Jin, Zhe,Teoh, Andrew Beng Jin,Goi, Bok-Min,Tay, Yong-Haur Elsevier 2016 Pattern recognition Vol.56 No.-

<P><B>Abstract</B></P> <P>Despite fuzzy commitment (FC) is a theoretically sound biometric-key binding scheme, it relies on error correction code (ECC) completely to mitigate biometric intra-user variations. Accordingly, FC suffers from the security–performance tradeoff. That is, the larger key size/higher security always trades with poor key release success rate and vice versa. Additionally, the FC is highly susceptible to a number of security and privacy attacks. Furthermore, the best achievable accuracy performance of FC is constrained by the simple distance metrics such as Hamming distance to measure the dissimilarity of binary biometric features. This implies many efficient matching algorithms are to be abandoned. In this paper, we propose an ECC-free key binding scheme along with cancellable transforms for minutiae-based fingerprint biometrics. Apart from that, the minutiae information is favorably protected by a strong non-invertible cancellable transform, which is crucial to prevent a number of security and privacy attacks. The scheme is not limited to binary biometrics as demanded in FC but instead can be applied to various types of biometric features and hence a more effective matcher can be chosen. Experiments conducted on FVC2002 and FVC2004 show that the accuracy performance is comparable to state-of-the-arts. We further demonstrate that the proposed scheme is robust against several major security and privacy attacks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new ECC-free biometric key binding scheme and the realization in fingerprint biometrics are proposed. </LI> <LI> A modified randomized GHE in constructing the cancellable transform is proposed. </LI> <LI> We performed several security and privacy analysis for the proposed scheme, like privacy attacks ARM and SKI. </LI> <LI> The proposed scheme can be applied to variety of biometric feature representations, not only binary string and matcher. </LI> </UL> </P>

TP53BP1 Regulates Chromosome Alignment and Spindle Bipolarity by Controlling MTOCs during Meiosis in Oocytes

Zhe-Long Jin,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

Meiotic oocytes lack classic centrosomes; therefore, bipolar-spindle assembly depends on the clustering of acentriolar microtubule-organizing centers (MTOCs) into two poles. Tumor suppressor P53-binding protein 1 (TP53BP1) is a known mediator in DNA damage response. Recent studies have revealed a new role of TP53BP1 in maintaining centrosome integrity in mitosis. However, the role of TP53BP1 in oocyte meiosis is unclear. Our results show that TP53BP1 participates in DNA damage responses in oocyte, as well as stablishment of chromosome alignment and spindle bipolarity by controlling the clustering of MTOCs during oocyte maturation. TP53BP1 was localized in the cytoplasm and concentrated around the spindle/chromosome region. Knockdown of TP53BP1 induced abnormal DNA damage response involving RAD51 and formation of γH2AX foci. TP53BP1 was also required for the clustering of MTOCs into two spindle poles during pro-meiosis I. Deletion of TP53BP1 induced perturbation in MTOC-localized Aurora Kinase A (AURKA). The knockdown of TP53BP1 induced microtubules to dis-attach from the kinetochores and increased the rate of aneuploidy. Taken together, our data show that TP53BP1 plays crucial roles in chromosome stability and spindle bipolarity during meiotic maturation by regulating the clustering of MTOCs.

Biodegradable Poly(lactic-co-glycolic acid) Maxpol-T/S as Novel Scaffold for Adipose Derived Stem Cells and Fibroblast Growth In Vitro

( Zhe Jin ),( Yan Qing Gong ),( Xin Cheng Qin ),( Jian Zhang ),( Yi Chen Zhu ),( Gui Ting Lin ),( Tom F Lue ),( Zhong Cheng Xin ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

To explore the ability of MaxPol-T/S, a novelbiodegradable poly-lactic-co-glycolic acid(PLGA) scaffold, in providing a cell-matrix interaction interface for cell growth, we generated GFP positive adipose derived stem cell(ADSCGFP+) and fibroblasts(FCGFP+) for investigating the cellular growth on this biomaterial. The MaxPol-T/S was produced through salt-leaching/particulate-leaching technology for tissue engineering, which provides a modified surface for the best cellular attachment sites and nutrient supply and waste exchange conditions. The morphological features of MaxPol-T/S were studied with a scanning electron microscope and the ADSCGFP+ and FCGFP+ were confirmed by auto-fluorescence microscopy and flow cytometry assay. Being seeded onto the MaxPol-T/S in vitro, the growth and morphology of ADSCGFP+ and FCGFP+ were further verified by auto-fluorescence microscopy and MMT test. The ADSCs and fibroblasts, expressed strong GFP signals in the cytoplasm and nucleus, adhered and proliferated on the surface of scaffold MaxPol-T/S. Both cell lines survived on the scaffold more than 21 days in vitro and formed three-dimensional colonies on the surface of the MaxPol-T. In conclusion, MaxPol-T/S is a novel PLGA scaffold for ADSCGFP+ and FCGFP+ and implies a promising technique for tissue engineering.

Dryocrassin ABBA Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells Through a Caspase-Dependent Mitochondrial Pathway

Jin, Zhe,Wang, Wen-Fei,Huang, Jian-Ping,Wang, He-Meng,Ju, Han-Xun,Chang, Ying Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Biological and pharmacological activities of dryocrassin ABBA, a phloroglucinol derivative extracted from Dryopteris crassirhizoma, have attracted attention. In this study, the apoptotic effect of dryocrassin ABBA on human hepatocellular carcinoma HepG2 cells was investigated. Materials and Methods: We tested the effects of dryocrassin ABBA on HepG2 in vitro by MTT, flow cytometry, real-time PCR, and Western blotting. KM male mice were used to detect the effect of dryocrassin ABBA on H22 cells in vivo. Results: Dryocrassin ABBA inhibited the growth of HepG2 cells in a concentration-dependent manner. After treatment with 25, 50, and $75{\mu}g/mL$ dryocrassin ABBA, the cell viability was 68%, 60% and 49%, respectively. Dryocrassin ABBA was able to induce apoptosis, measured by propidium iodide (PI)/annexin V-FITC double staining. The results of real-time PCR and Western ting showed that dryocrassin ABBA up-regulated p53 and Bax expression and inhibited Bcl-2 expression which led to an activation of caspase-3 and caspase-7 in the cytosol, and then induction of cell apoptosis. In vivo experiments also showed that dryocrassin ABBA treatment significantly suppressed tumor growth, without major side effects. Conclusions: Overall, these findings provide evidence that dryocrassin ABBA may induce apoptosis in human hepatocellular carcinoma cells through a caspase-mediated mitochondrial pathway.