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RISS 인기검색어
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- 저자 : Zhaohui Fang (검색결과 3 건)
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PENG AN,FANG ZUO,XINHUA LI,YUANPENG WU,JUNHUA ZHANG,ZHAOHUI ZHENG,XIAOBIN DING,YUXING PENG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6
A biomimetic and facile approach for integrating Fe3O4 and Au with polydopamine (PDA) was proposed to construct gold-coated Fe3O4 nanoparticles (Fe3O4@Au–PDA) with a core–shell structure by coupling in situ reduction with a seed-mediated method in aqueous solution at room temperature. The morphology, structure and composition of the core–shell structured Fe3O4@Au–PDA nanoparticles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD) and X-ray photoelectron spectrometry (XPS). The formation process of Au shell was assessed using a UV-Vis spectrophotometer. More importantly, according to investigating changes in PDA molecules by Fourier transform infrared spectroscopy (FTIR) and in preparation process of the zeta-potential data of nanoparticles, the mechanism of core–shell structure formation was proposed. Firstly, PDA-coated Fe3O4 are obtained using dopamine (DA) self-polymerization to form thin and surface-adherent PDA films onto the surface of a Fe3O4 "core". Then, Au seeds are attached on the surface of PDA-coated Fe3O4 via electrostatic interaction in order to serve as nucleation centers catalyzing the reduction of Au3+ to Au0 by the catechol groups in PDA. Accompanied by the deposition of Au, PDA films transfer from the surface of Fe3O4 to that of Au as stabilizing agent. In order to confirm the reasonableness of this mechanism, two verification experiments were conducted. The presence of PDA on the surface of Fe3O4@Au–PDA nanoparticles was confirmed by the finding that glycine or ethylenediamine could be grafted onto Fe3O4@Au–PDA nanoparticles through Schiff base reaction. In addition, Fe3O4@Au–DA nanoparticles, in which DA was substituted for PDA, were prepared using the same method as that for Fe3O4@Au–PDA nanoparticles and characterized by UV-Vis, TEM and FTIR. The results validated that DA possesses multiple functions of attaching Au seeds as well as acting as both reductant and stabilizing agent, the same functions as those of PDA.
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Wenquan Qin,Hongpeng Xiong,YuzhenWen,XiujunWen,HuiWang,Yihan Fang,TaoMa,Zhaohui Sun,Xiaoyang Chen,Cai Wang 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Baiting is widely applied for the detection and control of the red imported fire ants, Solenopsis invicta Buren. Adding preservatives into baits would prevent microbial contamination during production and storage, and might extend period of bait attractiveness in the field. However, it is not known if preservatives are repellent to S. invicta. In the present study, potential effects of six commonly used preservatives (potassiumsorbate, sodium benzoate, sodium diacetate, sodium dehydroacetate, calcium propionate, and nisin) on the foraging behaviors of S. invicta foragers were evaluated under laboratory and field conditions. Four-choice tests (laboratory study) showed that the number of foraging ants on the preservative-treated (immersed in 2000-, 5000-, or 10,000-ppm solution for 30 min) and control (immersed in distilled water for 30 min) food was similar, with an exception of sodium dehydroacetate, which significantly decreased the number of foraging ants at the three tested concentrations. Interestingly, 46%, 58% and 71% of the food items treated with 2000-, 5000- and 10,000- ppm solutions of sodium dehydroacetate were buried with soil particles, respectively, whereas only 17% of the control food was similarly buried. Multiple-choice tests (field study) showed that food treated with the 5000- or 10,000-ppm solution of sodium dehydroacetate attracted significantly fewer S. invicta workers compared to other preservatives and the controls. Our study showed that the use of sodiumdehydroacetate should be avoided during the production of aqueous-based or liquid baits against S. invicta.
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Screening nephroprotective compounds from cortex Moutan by mesangial cell extraction and UPLC
Min Sun,Limei Huang,Jianliang Zhu,Wenjie Bu,Jing Sun,Zhaohui Fang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6
A method for screening nephroprotective compoundsin cortex Moutan, a common traditional Chinesemedicine (TCM) in treating diabetic nephropathy with renalmesangial cell extraction and ultra performance liquidchromatography technique was described in this paper. Wehypothesize that the compounds which bind to cell membranesunder pathological conditions may be the bioactivecompounds in TCMs. Mesangial cells were cultured inmedium containing 5 mM (physiological, NG) or 30 mM(pathological, HG) glucose for 48 h and then incubated withcortex Moutan extract. After the unbound substances werewashed off, the cell membrane-bound compounds weredissociated and concentrated by an SPE column. By comparingthe chromatograms of NG and HG cultured-cellextractions and cortex Moutan extract, three compoundsbound to both NG and HG-cultured mesangial cells wereidentified as paeoniflorin, pentagalloylglucose (PGG) andpaeonol. In vitro studies showed that paeoniflorin, PGG andpaeonol reduced the activity of nicotinamide-adenine dinucleotidephosphate oxidase (NADPH) activity, anddecreased the level of reactive oxygen species (ROS),transforming growth factor-b1 (TGF-b1) and fibronectin inhigh glucose cultured mesangial cells. The results indicatethat paeonol, paeoniflorin and PGG may be the nephroprotectivecompounds from cortex Moutan. This study isexpected to provide a more reliable and effective method forscreening bioactive compounds from the complex TCM systems.
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