miR-340 Reverses Cisplatin Resistance of Hepatocellular Carcinoma Cell Lines by Targeting Nrf2-dependent Antioxidant Pathway

Shi, Liang,Chen, Zhan-Guo,Wu, Li-li,Zheng, Jian-Jian,Yang, Jian-Rong,Chen, Xiao-Fei,Chen, Zeng-Qiang,Liu, Cun-Li,Chi, Sheng-Ying,Zheng, Jia-Ying,Huang, Hai-Xia,Lin, Xiang-Yang,Zheng, Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Many chemotherapeutic agents have been successfully used to treat hepatocellular carcinoma (HCC); however, the development of chemoresistance in liver cancer cells usually results in a relapse and worsening of prognosis. It has been demonstrated that DNA methylation and histone modification play crucial roles in chemotherapy resistance. Currently, extensive research has shown that there is another potential mechanism of gene expression control, which is mediated through the function of short noncoding RNAs, especially for microRNAs (miRNAs), but little is known about their roles in cancer cell drug resistance. In present study, by taking advantage of miRNA effects on the resistance of human hepatocellular carcinoma cells line to cisplatin, it has been demonstrated that miR-340 were significantly downregulated whereas Nrf2 was upregulated in HepG2/CDDP (cisplatin) cells, compared with parental HepG2 cells. Bioinformatics analysis and luciferase assays of Nrf2-3'-untranslated region-based reporter constructor indicated that Nrf2 was the direct target gene of miR-340, miR-340 mimics suppressing Nrf2-dependent antioxidant pathway and enhancing the sensitivity of HepG2/CDDP cells to cisplatin. Interestingly, transfection with miR-340 mimics combined with miR-340 inhibitors reactivated the Nrf2 related pathway and restored the resistance of HepG2/CDDP cells to CDDP. Collectively, the results first suggested that lower expression of miR-340 is involved in the development of CDDP resistance in hepatocellular carcinoma cell line, at least partly due to regulating Nrf2-dependent antioxidant pathway.

Finite Element and Experimental Analysis of Ultrasonic Vibration Milling of High-Volume Fraction SiCp/Al Composites

Zhan-Li Shi,Dao-Hui Xiang,Hao-Ren Feng,Bang-Fu Wu,Zhi-Meng Zhang,Guo-Fu Gao,Bo Zhao 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.10

SiCp/Al composites have been widely used in many fields due to their excellent mechanical properties. However, the addition of reinforced phase SiC particles makes the overall properties of the composites hard and brittle, which brings great challenges to milling. Ultrasonic vibration-assisted processing technology has great advantages in processing hard and brittle materials. However, the process of rupture of SiC particles cannot be effectively observed during the test processing, and a large number of tests increase the cost of the test. The combination of finite element analysis and experiment was used to study the machining performance of High-volume fraction SiCp/Al composites in longitudinal-torsional ultrasonicassisted milling (LTUAM), and its feasibility was evaluated by comparing with conventional Milling (CM). By analyzing the trajectories of cutting edges in ultrasonic-assisted milling, It was found that ultrasonic frequency determines the time of periodic contact-separation between chisel edge and workpiece, and ultrasonic amplitude determines the maximum distance of contact-separation. Using ABAQUS finite element software, a polygon SiC particles model with a high-volume fraction was established to investigate the SiC particles crushing process under different ultrasonic-assisted milling conditions. The results showed that high-frequency ultrasonic-assisted milling could soften SiCp/Al composites, and the structural integrity of silicon carbide particles could be better maintained under appropriate ultrasonic amplitudes, reducing the probability of fragmentation. The removal mode was mainly plastic removal or crushing into small particles. The surface roughness value and milling force were reduced, improving the surface quality of the processed composite material. The conclusions of the milling test were basically consistent with the simulation results, which prove the correctness and feasibility of the simulation results

Performance of Hepatitis B Core-Related Antigen Versus Hepatitis B Surface Antigen and Hepatitis B Virus DNA in Predicting HBeAg-positive and HBeAg-negative Chronic Hepatitis

Zhan-qing Zhang,Yan-bing Wang,,Wei Lu,,Dan-ping Liu,,Bi-sheng Shi,,Xiao-nan Zhang,,Dan Huang,,Xiu-fen Li,,Xin-lan Zhou,,Rong-rong Ding, 대한진단검사의학회 2019 Annals of Laboratory Medicine Vol.39 No.1

Background: We examined changes in hepatitis B core-related antigen (HBcrAg) during the four sequential phases of chronic hepatitis B virus (HBV) infection: hepatitis B e antigen (HBeAg)-positive chronic infection (EPCI) and hepatitis (EPCH), followed by HBeAg-negative chronic infection (ENCI) and hepatitis (ENCH). We compared the performance of serum HBcrAg, hepatitis B surface antigen (HBsAg), and HBV DNA in predicting EPCH and ENCH.

Muscle-derived Stem Cells Differentiate into Functional Smooth Muscle Cells for Ureter Tissue Engineering: An Experimental Study

Zhan-Kui Zhao,Hong-Lian Yu,Fei Xiao,Shi-Wen Li,Wen-Biao Liao,Kai-Liang Zhao 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

We assessed the ability of muscle-derived stem cells (MDSC) to differentiate into smooth muscle cells (SMC) and their potential to promote the regeneration of smooth muscle with a vessel extracellular matrix (VECM)for tissue engineering of the ureter. MDSC were isolated,proliferated, and identified by flow cytometry. SMC phenotype differentiation was induced with a smooth muscle induction medium. Gene expression was evaluated by real-time quantitative polymerase chain reaction (PCR)and Western blot studies. The VECM was obtained by a decellularization process, and cytotoxic effects were evaluated by exposing the induced cells to a VECM extract. The induced cells were seeded onto VECM in vitro for 1 week, and then the compound grafts were used for ureter reconstitution in vivo. The grafts were obtained for histological studies at 2, 4, 8, and 16 weeks post-operation. Intravenous urography was used to evaluate renal function and ureteral patency. Flow cytometry demonstrated that the MDSC expressed Sca-1 and desmin, but did not express CD45. After induction, SMC phenotype gene expression was confirmed in the induced cells by real-time quantitative PCR and Western blot studies. VECM exhibited a nontoxic effect on the induced cells in vitro. At 16 weeks postoperation,a histological evaluation showed that multilayered urothelium and organized muscle fiber bundles had formed in the grafts. Intravenous urography demonstrated no evidence of ureteral stricture or hydroureteronephrosis. These results demonstrate that MDSC can be induced into SMC and that this was useful for promoting regeneration of smooth muscles for ureter tissue engineering. We assessed the ability of muscle-derived stem cells (MDSC) to differentiate into smooth muscle cells (SMC) and their potential to promote the regeneration of smooth muscle with a vessel extracellular matrix (VECM)for tissue engineering of the ureter. MDSC were isolated,proliferated, and identified by flow cytometry. SMC phenotype differentiation was induced with a smooth muscle induction medium. Gene expression was evaluated by real-time quantitative polymerase chain reaction (PCR)and Western blot studies. The VECM was obtained by a decellularization process, and cytotoxic effects were evaluated by exposing the induced cells to a VECM extract. The induced cells were seeded onto VECM in vitro for 1 week, and then the compound grafts were used for ureter reconstitution in vivo. The grafts were obtained for histological studies at 2, 4, 8, and 16 weeks post-operation. Intravenous urography was used to evaluate renal function and ureteral patency. Flow cytometry demonstrated that the MDSC expressed Sca-1 and desmin, but did not express CD45. After induction, SMC phenotype gene expression was confirmed in the induced cells by real-time quantitative PCR and Western blot studies. VECM exhibited a nontoxic effect on the induced cells in vitro. At 16 weeks postoperation,a histological evaluation showed that multilayered urothelium and organized muscle fiber bundles had formed in the grafts. Intravenous urography demonstrated no evidence of ureteral stricture or hydroureteronephrosis. These results demonstrate that MDSC can be induced into SMC and that this was useful for promoting regeneration of smooth muscles for ureter tissue engineering.

Predictive control with optimal vector sequence for permanent magnet synchronous motors

Li, Chen,Shi, Ting-Na,Yan, Yan,Zhou, Zhan-Qing,Xia, Chang-Liang The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.2

Conventional model predictive control aims to minimize ripples of the torque, stator flux and current at the end instant of each control period. However, the trajectories of the torque and flux during the entire control period are changed when multiple voltage vectors are applied in different sequences in each period. This indicates that the ripples of the torque and stator flux are influenced by different vector sequences (i.e., the sequence multiple vectors applied in). For that issue, this paper analyzes the RMS values of the stator flux vector ripple when three basic vectors are applied in different five-segment sequences, and proposes a predictive control with optimal vector sequence method. Based on this analysis, the available vector sequences that reduce the stator flux vector ripple are screened out. Moreover, a cost function is constructed to select the optimal vector sequence by evaluating the stator flux ripple during the entire control period and the tracking error of the stator flux vector at the end instant of each control period. Finally, simulation and experimental results verify the effectiveness of the proposed method.

Determination of equivalent blasting load considering millisecond delay effect

Song, Zhan-Ping,Li, Shi-Hao,Wang, Jun-Bao,Sun, Zhi-Yuan,Liu, Jing,Chang, Yu-Zhen Techno-Press 2018 Geomechanics & engineering Vol.15 No.2

In the analysis of the effects of rock tunnel blasting vibration on adjacent existing buildings, the model of simplified equivalent load produces higher calculation result of vibration, due to the lack of consideration of the millisecond delay effect. This paper, based on the static force equivalence principle of blasting load, proposes a new determination method of equivalent load of blasting vibration. The proposed method, based on the elastic-static force equivalence principle of stress wave, equals the blasting loads of several single blastholes in the same section of millisecond blasting to the triangle blasting load curve of the exploded equivalent elastic boundary surface. According to the attenuation law of stress wave, the attenuated equivalent triangle blasting load curve of the equivalent elastic boundary is applied on the tunnel excavation contour surface, obtaining the final applied equivalent load. Taking the millisecond delay time of different sections into account, the time-history curve of equivalent load of the whole section applied on the tunnel excavation contour surface can be obtained. Based on Sailing Tunnel with small spacing on Sanmenxia-Xichuan Expressway, an analysis on the blasting vibration response of the later and early stages of the tunnel construction is carried out through numerical simulation using the proposed equivalent load model considering millisecond delay effect and the simplified equivalent triangle load curve model respectively. The analysis of the numerical results comparing with the field monitoring ones shows that the calculation results obtained from the proposed equivalent load model are closer to the measured ones and more feasible.

Effect of Metabolic Structures and EnergyRequirements on Curdlan Production byAlcaligenes faecalis

Xiao Bei Zhan,Zhi-Yong Zheng,Jin Woo Lee,Zhongping Shi,Lei Wang,Li Zhu,Jian-Rong Wu,Chi Chung Lin 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.4

A comprehensive metabolic network was proposed for Alcaligenes faecalis and employed in a stoichiometrically based flux balance model for curdlan production optimization. The maximal yield of curdlan was evaluated for curdlan batch production. Various metabolic structures and metabolic pathway distributions related with the curdlan maximal yield was evaluated. The results showed that the energy efficiency rather than the substrate supply was the major constraint for the enhancement of curdlan production. The increase in specific rate of glucose uptake could enhance curdlan production yield due to the decrease of the ratio of metabolic maintenance to substrate consumption. However, some of the energy loss and nutrient limitation associated with the increase of metabolic maintenance would adversely affect the conversion efficiency of the substrate.

Quantitative Presentation of the Systematic Relationships Between S. sudanense and S. bicolor

Qiu-Wen Zhan,Ping Lin,Jie-Qin Li,Min Zu,Jun-Cheng Fan,Ya Chen,Fei Fan,Shi-fu Shu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Fifty-four biological characters were used for quantitative classification of sorghum and sudangrass and their relationship. Discriminant analysis of all 240 individuals of 48 accessions suggested that 100% of originally grouped cases were correctly classified. Analysis of variance results showed that the differences among 48 accessions in characters were significant at the 0.01 level. Cluster analysis showed that the 48 accessions were obviously divided into two clusters by a Euclidean distance of 17.46 as the classification standards, i.e. the genus maize and genus sorghum. The 48 accessions were divided into four clusters by a Euclidean distance of 9.86, i.e. Sorghum-Sudangrass, Sorghum related species (S. propinquum and S. halepense), Zea mexicana and Z. mays. All accessions were divided into five clusters by a Euclidean distance of 8.91, namely group Ⅰ (Sudangrass-Chinese sorghum), group Ⅱ (Non-Chinese sorghum), group Ⅲ (Sorghum related species), group Ⅳ (Zea mexicana), and group Ⅴ (Z mays).

Physiological and metabolic responses to nitrogen availability of rice (Oryza sativa L.) cultivars with differ in nitrogen efficient

Ruan Xinmin,Du Hongyang,Zhan Xinchun,Cong Xihan,Shi Fuzhi,Li Juan,Luo Zhixiang,Dong Zhaorong 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.1

Developing genotypes with high-nitrogen use efficiency is a prerequisite for achieving sustainable agriculture and high yields. In this study, both physiological and metabolic analyses were performed to evaluate the NUE (nitrogen use efficiency) of two elite indica rice cultivars: OM052 and Huanghuazhan (HHZ). The pNUE and gNUE of OM052 were higher than HHZ under three N conditions, and significant (p < 0.05) especially under both LN and MN conditions. The contents of soluble sugar and malate were significantly different between OM052 and HHZ under LN and HN conditions. The GDH activity in OM052 was significantly high than that in HHZ under HN condition. Metabolic analyses showed that 136 and 142 differentially accumulating metabolites were identified between OM052 and HHZ under LN and HN conditions, respectively. KEGG analysis showed that ‘aminoacyl-tRNA biosynthesis’ was also the most significant enriched in LN_OM052 vs. LN_ HHZ and ‘Glyoxylate and dicarboxylate metabolism’ and ‘citrate cycle’ were the most significantly enriched in HN_OM052 vs. HN_ HHZ. The contents of four metabolites (cis-aconitate, isocitrate, fumarate, and malate) involved in the TCA cycle were significantly upregulated in HN condition compared with LN condition, regardless of OM052 and HHZ. Taken together, the results of our study revealed the physiological and metabolic differences underlying the OM052 and HHZ and provide new insights into coordinating C and N metabolism and improving the NUE of rice.

Chinese Society of Allergy and Chinese Society of Otorhinolaryngology-Head and Neck Surgery Guideline for Chronic Rhinosinusitis

Zheng Liu,Jianjun Chen,Lei Cheng,Huabin Li,Shixi Liu,Hongfei Lou,Jianbo Shi,Ying Sun,Dehui Wang,Chengshuo Wang,Xiangdong Wang,Yongxiang Wei,Weiping Wen,Pingchang Yang,Qintai Yang,Gehua Zhang,Yuan Zhan 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.2

The current document is based on a consensus reached by a panel of experts from the Chinese Society of Allergy and the Chinese Society of Otorhinolaryngology-Head and Neck Surgery, Rhinology Group. Chronic rhinosinusitis (CRS) affects approximately 8% of Chinese adults. The inflammatory and remodeling mechanisms of CRS in the Chinese population differ from those observed in the populations of European descent. Recently, precision medicine has been used to treat inflammation by targeting key biomarkers that are involved in the process. However, there are no CRS guidelines or a consensus available from China that can be shared with the international academia. The guidelines presented in this paper cover the epidemiology, economic burden, genetics and epigenetics, mechanisms, phenotypes and endotypes, diagnosis and differential diagnosis, management, and the current status of CRS in China. These guidelines—with a focus on China—will improve the abilities of clinical and medical staff during the treatment of CRS. Additionally, they will help international agencies in improving the verification of CRS endotypes, mapping of eosinophilic shifts, the identification of suitable biomarkers for endotyping, and predicting responses to therapies. In conclusion, these guidelines will help select therapies, such as pharmacotherapy, surgical approaches and innovative biotherapeutics, which are tailored to each of the individual CRS endotypes.