NUMERICAL SIMULATION OF 2-D SHALLOW WATER FLOW OF THE WATER ENVIRONMENT IN ZHENJING CITY

ZHOU YILIN,TANG HONGWU,LIU TAO 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Molecular and Functional Characterization of ZmNF-YC14 in Transgenic Arabidopsis

Yilin Cai,Xiupeng Mei;Ping Li;Lu Wang;Chaoxian Liu;Lian Zhou 한국식물학회 2018 Journal of Plant Biology Vol.61 No.6

The optimum transition stage from vegetative toreproductive development is important for flowering plantsto obtain the desired plant architecture to maximize yield. Inthis study, we overexpressed the maize NUCLEAR FACTORY, SUBUNIT C 14 (ZmNF-YC14) gene in Arabidopsis toinvestigate its potential functions in the regulation of planttransition. Overexpression of ZmNF-YC14 in Arabidopsisinhibited plant flowering and retarded the duration of thebranch-producing inflorescence phase 1 (I1). These plantsexhibited increased length of I1 and higher ratio of inflorescencephase 1 to inflorescence phase (I) (I1:I) under long-dayconditions. As a consequence, these transgenic plants exhibiteddramatic changes in their overall inflorescence morphology. In addition, the phenotypes of inflorescence morphologycaused by ZmNF-YC14 overexpression were enhanced byexogenous gibberellin (GA) treatment, which obtained asignificant increase in I1:I, inflorescence phase 1 to inflorescencephase 2 (I2) (I1:I2), and remarkable decrease in I2 and I2:I intransgenic plants compared to those under normal conditions. Taken together, the results of this study suggest that ZmNFYC14negatively regulates flowering and controls flowerformation under long-day conditions in Arabidopsis and maybe involved in a GA regulation pathway.

One-Pot Synthesis of CdSe Quantum Dots Using Selenium Dioxide as a Selenium Source in Aqueous Solution

Yilin Wang,Hong Yang,Zhenyi Xia,Zhangfa Tong,Liya Zhou 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel technology has been developed for the synthesis of thioglycolic acid (TGA)-capped CdSe quantum dots (QDs) in aqueous medium. The reaction was carried out in air atmosphere with one-pot by using SeO_2 to replace Se or Na_2Se. The technological parameters including refluxing time, pH values and molar ratios of selenium to cadmium had significant influence on the luminescence properties of CdSe QDs. Furthermore, the obtained QDs were characterized by fluorescent spectroscopy, X-ray powder diffraction (XRD) and transmission electron microscopy (TEM), respectively. The results demonstrated that the CdSe QDs were of zinc-blended crystal structure in a sphere-like shape.

Potential implications of hydrogen peroxide in the pathogenesis and therapeutic strategies of gliomas

Ying Zhou,Long Wang,Chaojia Wang,Yilin Wu,Dongmei Chen,Tae Ho Lee 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.2

Glioma is the most common type of primarybrain tumor, and it has a high mortality rate. Currently,there are only a few therapeutic approaches for gliomas, andtheir effects are unsatisfactory. Therefore, uncovering thepathogenesis and exploring more therapeutic strategies forthe treatment of gliomas are urgently needed to overcomethe ongoing challenges. Cellular redox imbalance has beenshown to be associated with the initiation and progression ofgliomas. Among reactive oxygen species (ROS), hydrogenperoxide (H2O2) is considered the most suitable for redoxsignaling and is a potential candidate as a key moleculethat determines the fate of cancer cells. In this review, wediscuss the potential cellular and molecular roles of H2O2in gliomagenesis and explore the potential implications ofH2O2in radiotherapy and chemotherapy and in the ongoingchallenges of current glioma treatment. Moreover, we evaluateH2O2as a potential redox sensor and potential driver molecule of nanocatalytic therapeutic strategies for gliomatreatment.

One-Pot Synthesis of CdSe Quantum Dots Using Selenium Dioxide as a Selenium Source in Aqueous Solution

Wang, Yilin,Yang, Hong,Xia, Zhenyi,Tong, Zhangfa,Zhou, Liya Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel technology has been developed for the synthesis of thioglycolic acid (TGA)-capped CdSe quantum dots (QDs) in aqueous medium. The reaction was carried out in air atmosphere with one-pot by using $SeO_2$ to replace Se or $Na_2Se$. The technological parameters including refluxing time, pH values and molar ratios of selenium to cadmium had significant influence on the luminescence properties of CdSe QDs. Furthermore, the obtained QDs were characterized by fluorescent spectroscopy, X-ray powder diffraction (XRD) and transmission electron microscopy (TEM), respectively. The results demonstrated that the CdSe QDs were of zinc-blended crystal structure in a sphere-like shape.

Classification of Motor Imagery Tasks for Electrocorticogram Based Brain-Computer Interface

Fangzhou Xu,Weidong Zhou,Yilin Zhen,Qi Yuan 대한의용생체공학회 2014 Biomedical Engineering Letters (BMEL) Vol.4 No.2

Purpose In the present study, we propose a novel schemefor motor imagery (MI) classification of multichannelelectrocorticogram (ECoG) recordings from patients withmedically intractable focal epilepsy. Methods This scheme proposes a combination of the twofeatures which includes autoregressive (AR) model coefficientsand local binary pattern (LBP) operators. It can providespatial resolution and angular space information. Then thegradient boosting (GB) in conjunction with ordinary leastsquares (OLS) algorithm is employed as the classifier toimprove the performance of MI classification for ECoGbased Brain Computer Interface (BCI) system. Results Experimental results on the BCI Competition IIIdata set I indicate that the novel method has excellentperformance and yields a cross-validation accuracy of 88.8%and accuracy of 93%, respectively. Conclusions From the experimental results and comparativestudies, we can infer that the scheme may serve as a good MIclassification tool for a better tradeoff between the classificationaccuracy and computational complexity.

Proliferated Leydig Cells for Engineered Testis-like Tissue Regeneration with Testosterone-Secreting Ability

Hongda Bi,Xiaoyun Wang,Wei Liu,Yilin Cao,Guangdong Zhou,Xin Xing 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.5

Tissue engineering approach provides a hopeful strategy for reconstructing testis testosterone-secreting functions. However, limited source and low proliferative activity in vitro of Leydig cells (LCs, the main testosteroneproducing cells) makes testis-like tissue regeneration difficult to be achieved. This study explored the feasibility of in vitro expanding LCs and their potential application in testis-like tissue regeneration. LC lineage cells were isolated from Sprague-Dawley (SD) rats by differential adhesion method and cell composition was identified by expressions of 3β-HSD, LHR, LIFR, and c-kit. A modified expansion medium (EM) system was used to test the feasibility of in vitro expanding LC lineage. The results showed that the attached cells reached a high purification of LC lineage (>90%, indicated by positive expression of 3β-HSD) and that EM significantly enhanced proliferation of LC lineage compared to regular medium, which was testified to be related to the presence of stem LCs that was implied by positive expressions of LIFR and c-kit as well as the transition of 3β-HSD expression from negative to positive in partial cells. Importantly, the proliferated LCs showed relatively sustained testosterone-secreting ability in vitro and these cells combined with biodegradable scaffolds successfully regenerated testis-like tissue with sustained testosteronesecreting function in vivo, which was supported by the enhanced serum testosterone level in castrated rats. All these results indicated that the differential adhesion method could efficiently isolate and purify LC lineage and that EM system could efficiently promote proliferation and functional maintenance of LC lineage, providing a good cell source for testes-like tissue regeneration. Tissue engineering approach provides a hopeful strategy for reconstructing testis testosterone-secreting functions. However, limited source and low proliferative activity in vitro of Leydig cells (LCs, the main testosteroneproducing cells) makes testis-like tissue regeneration difficult to be achieved. This study explored the feasibility of in vitro expanding LCs and their potential application in testis-like tissue regeneration. LC lineage cells were isolated from Sprague-Dawley (SD) rats by differential adhesion method and cell composition was identified by expressions of 3β-HSD, LHR, LIFR, and c-kit. A modified expansion medium (EM) system was used to test the feasibility of in vitro expanding LC lineage. The results showed that the attached cells reached a high purification of LC lineage (>90%, indicated by positive expression of 3β-HSD) and that EM significantly enhanced proliferation of LC lineage compared to regular medium, which was testified to be related to the presence of stem LCs that was implied by positive expressions of LIFR and c-kit as well as the transition of 3β-HSD expression from negative to positive in partial cells. Importantly, the proliferated LCs showed relatively sustained testosterone-secreting ability in vitro and thesecells combined with biodegradable scaffolds successfully regenerated testis-like tissue with sustained testosteronesecreting function in vivo, which was supported by the enhanced serum testosterone level in castrated rats. All these results indicated that the differential adhesion method could efficiently isolate and purify LC lineage and that EM system could efficiently promote proliferation and functional maintenance of LC lineage, providing a good cell source for testes-like tissue regeneration.

Bioinspired Fabrication and Evaluation of Molecularly Imprinted Nanocomposite Membranes with Inorganic/Organic Multilevel Structure for the Selective Separation of Emodin

Chao Yu,Jian Lu,Qi Zhang,Hougang Fan,Minjia Meng,Shi Zhou,Yinhua Jiang,Yongsheng Yan,Yilin Wu,Chunxiang Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.2

High purity emodin is in great demand with the development of medical treatment. Molecularly imprinted membranes (MIMs) have gained wide attention for selective separation of emodin due to its preferable selectivity. In this work, we describe a simple two-step method for developing emodin-imprinted TiO2@CA (ETMIMs) and emodin-imprinted SiO2@CA (ESMIMs) based on organic–inorganic nanoparticle (SiO2/TiO2) modified cellulose acetate membranes at room temperature. SiO2/TiO2 is used to improve the structural stability and roughness of membranes, and dopamine is used as the functional monomer and crosslinker. Importantly, the as-prepared membranes not only exhibited enhanced rebinding capacity (ETMIMs = 30.73 mg g -1 and ESMIMs = 46.04 mg g -1) but also possessed superior rebinding selectivity (2.76 and 2.99 for physcion and 2.42 and 3.30 for aloe emodin onto ETMIMs and ESMIMs) as well as permselectivity (7.59 and 6.69 for physcion and 5.94 and 5.78 for aloe emodin onto ETMIMs and ESMIMs). The regeneration ability of ETMIMs and ESMIMs still maintained 90.4% and 89.2% of the original rebinding capacity after 10 cycling steps, respectively. The ETMIMs and ESMIMs obtained in this work show potential applications for selective separation and purification of emodin from analogs.

Chondrogenic Differentiation and Three Dimensional Chondrogenesis of Human Adipose-Derived Stem Cells Induced by Engineered Cartilage-Derived Conditional Media

Hengyun Sun,Yu Liu,Ting Jiang,Xia Liu,Aijuan He,Jie Li,Wenjie Zhang,Wei Liu,Yilin Cao,Guangdong Zhou 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1

Due to lack of optimal inductive protocols, how to effectively improve chondrogenesis of adipose-derived stem cells (ASCs) is still a great challenge. Our previous studies demonstrated that the culture media derived from chondrocyte-scaffold constructs (conditional media) contained various soluble chondrogenic factors and were effective for directing chondrogenic differentiation of bone marrow stem cells. Nevertheless, it remains unclear whether the conditional media can induce ASCs towards chondrogenic differentiation, especially for three-dimensional (3D) cartilage formation in a preshaped scaffold. In this study, it demonstrated that the conditional media derived from chondrocyte-scaffold constructs could promote ASCs to differentiate into chondrocyte-like cells, with similar expression of type II collagen to those induced by chondrogenic growth factors. Moreover, the expression level of chondrocyte-specific genes, such as SOX9, type II collagen, and COMP, was even higher in conditional medium group (CM) than that in optimized chondrogenic growth factor group (GF), indicating that the conditional media can serve as an effective inducer for chondrogenic differentiation of ASCs. Most importantly, the conditional media could also induce ASC-scaffold constructs to form 3D cartilage-like tissue with typical lacunae structures and positive expression of cartilage specific matrices, even higher contents of GAG and type II collagen were achieved in CM group compared to GF group. The current study establishes a simple, but stable, efficient, and economical method for directing 3D cartilage formation of ASCs, a strategy that may be more closely applicable for repairing cartilage defects.