Ginsenoside compound K inhibits nuclear factor-kappa B by targeting Annexin A2

Yushi Wang,Hongyan Zhu,He Liu,Yang Li,Bing Zhao,Ying-Hua Jin 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.3

Background: Ginsenoside compound K(C-K), a major metabolite of ginsenoside, exhibits anticancer activityin various cancer cells and animal models. A cell signaling study has shown that C-K inhibitednuclear factor-kappa B (NF-kB) pathway in human astroglial cells and liver cancer cells. However, themolecular targets of C-K and the initiating events were not elucidated. Methods: Interaction between C-K and Annexin A2 was determined by molecular docking and thermalshift assay. HepG2 cells were treated with C-K, followed by a luciferase reporter assay for NF-lB,immunofluorescence imaging for the subcellular localization of Annexin A2 and NF-lB p50 subunit,coimmunoprecipitation of Annexin A2 and NF-lB p50 subunit, and both cell viability assay and plateclone formation assay to determine the cell viability. Results: Both molecular docking and thermal shift assay positively confirmed the interaction betweenAnnexin A2 and C-K. This interaction prevented the interaction between Annexin A2 and NF-lB p50subunit and their nuclear colocalization, which attenuated the activation of NF-lB and the expression ofits downstream genes, followed by the activation of caspase 9 and 3. In addition, the overexpression ofAnnexin A2-K320A, a C-K binding-deficient mutant of Annexin A2, rendered cells to resist C-K treatment,indicating that C-K exerts its cytotoxic activity mainly by targeting Annexin A2. Conclusion: This study for the first time revealed a cellular target of C-K and the molecular mechanismfor its anticancer activity.

A Parametric Study of the Functioning of an Axisymmetric Explosive Separation Device

Guangyu Wang,Quan Wen,Xiaopeng Wang,Yushi Wang 한국항공우주학회 2021 International Journal of Aeronautical and Space Sc Vol.22 No.6

At present, the design and optimization of the linear explosive separation device mainly depends on experiment, which is not only costly but also low efficient. The introduction of finite element analysis prior to experiment may reduce the cost to design a linear explosive separation device. Herein, arbitrary Lagrangian–Eulerian (ALE) numerical method is utilized to investigate the functioning of an axisymmetric explosive separation device. Firstly, several ALE models are validated via explosive separation experiments and photonic Doppler velocimetry (PDV) measurements, and found to have reasonable accuracy. Then, a series of ALE models are developed to study the transient separation process of an axisymmetric explosive separation device. Multiple factors which may influence the process are identified through parametric study. Especially, it is found that the linear density of the explosive core in the detonating cord and the bottom radius of the v-notch on the separation plate are critical for the accumulation of effective plastic strain at the v-notch. In addition, the material of the protection plate and the angle of the v-notch have a certain influence on the effective plastic strain at the v-notch. The material strength of the protection plate almost has little influence on the effective plastic strain at the v-notch. The study may facilitate the optimal design of the axisymmetric explosive separation device.

Radix et Rhizoma Ginseng chemoprevents both initiation and promotion of cutaneous carcinoma by enhancing cell-mediated immunity and maintaining redox homeostasis

Yu, Suyun,Wang, Siliang,Huang, Shuai,Wang, Wei,Wei, Zhonghong,Ding, Yushi,Wang, Aiyun,Huang, Shile,Chen, Wenxing,Lu, Yin The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.4

Background: Radix et Rhizoma Ginseng (thereafter called ginseng) has been used as a medicinal herb for thousands of years to maintain people's physical vitality and is also a non-organ-specific cancer preventive and therapeutic traditional medicine in several epidemiologic and preclinical studies. Owing to few toxic side effects and strong enhancement on body immunity, ginseng has admirable application potential and value in cancer chemoprevention. The study aims at investigating the chemopreventive effects of ginseng on cutaneous carcinoma and the underlying mechanisms. Methods: The mouse skin cancer model was induced by 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate. Ultraperformance liquid chromatography/mass spectrometry was used for identifying various ginsenosides, the main active ingredients of ginseng. Comprehensive approaches (including network pharmacology, bioinformatics, and experimental verification) were used to explore the potential targets of ginseng. Results: Ginseng treatment inhibited cutaneous carcinoma in terms of initiation and promotion. The content of Rb1, Rb2, Rc, and Rd ginsenosides was the highest in both mouse blood and skin tissues. Ginseng and its active components well maintained the redox homeostasis and modulated the immune response in the model. Specifically, ginseng treatment inhibited the initiation of skin cancer by enhancing T-cell-mediated immune response through upregulating HSP27 expression and inhibited the promotion of skin cancer by maintaining cellular redox homeostasis through promoting nuclear translocation of Nrf2. Conclusion: According to the study results, ginseng can be potentially used for cutaneous carcinoma as a chemopreventive agent by enhancing cell-mediated immunity and maintaining redox homeostasis with multiple components, targets, and links.

Radix et Rhizoma Ginseng chemoprevents both initiation and promotion of cutaneous carcinoma by enhancing cell-mediated immunity and maintaining redox homeostasis

Suyun Yu,Siliang Wang,Shuai Huang,Wei Wang,Zhonghong Wei,Yushi Ding,Aiyun Wang,Shile Huang,Wenxing Chen,Yin Lu 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4

Background: Radix et Rhizoma Ginseng (thereafter called ginseng) has been used as a medicinal herb forthousands of years to maintain people’s physical vitality and is also a noneorgan-specific cancer preventiveand therapeutic traditional medicine in several epidemiologic and preclinical studies. Owing tofew toxic side effects and strong enhancement on body immunity, ginseng has admirable applicationpotential and value in cancer chemoprevention. The study aims at investigating the chemopreventiveeffects of ginseng on cutaneous carcinoma and the underlying mechanisms. Methods: The mouse skin cancer model was induced by 7,12-dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate. Ultraperformance liquid chromatography/mass spectrometry was used foridentifying various ginsenosides, the main active ingredients of ginseng. Comprehensive approaches(including network pharmacology, bioinformatics, and experimental verification) were used to explorethe potential targets of ginseng. Results: Ginseng treatment inhibited cutaneous carcinoma in terms of initiation and promotion. Thecontent of Rb1, Rb2, Rc, and Rd ginsenosides was the highest in both mouse blood and skin tissues. Ginseng and its active components well maintained the redox homeostasis and modulated the immuneresponse in the model. Specifically, ginseng treatment inhibited the initiation of skin cancer byenhancing T-cellemediated immune response through upregulating HSP27 expression and inhibited thepromotion of skin cancer by maintaining cellular redox homeostasis through promoting nuclear translocationof Nrf2. Conclusion: According to the study results, ginseng can be potentially used for cutaneous carcinoma as achemopreventive agent by enhancing cell-mediated immunity and maintaining redox homeostasis withmultiple components, targets, and links.

Finite-time Incremental Stability Analysis for Nonlinear Switched Systems With Unstable Subsystems

Lijuan Wang,Yuanhong Ren,Yushi Yang,Lin Guan 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

In this paper, finite-time incremental stability (FTIS) and finite-time incremental boundedness (FTIB) are investigated for nonlinear switched systems (NSS) with unstable systems. Firstly, based on the reverse modedependent average dwell time (MDADT) method, we propose some sufficient conditions for FTIS of NSS. Secondly, the sufficient conditions for NSS to be FTIB with an incremental performance index are given by the multiple incremental Lyapunov functions. Finally, a distinctive advantage of reverse MDADT method is that each subsystems satisfies FTIB in the activation interval. A example has been provided to show the effectiveness of the theoretic results developed.

Rapid Microwave Irradiation Synthesis and Photoelectrochemical Performance of Cu3BiS3 nanoparticles

Wei Wang,Guowei Zhi,Liu Liu,Yujie  Fei,QinYang Sheng,Yushi Shi,Hanyu Yao,Hongqiang Qian 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.6

A photocatalytic material with high performance and low cost plays a decisive role in the industrialization of photocatalytichydrogen evolution. In this work, Cu3BiS3 nanoparticles were successfully synthesized by the microwave irradiationmethod, and the formation mechanism of Cu3BiS3 was studied. The Cu3BiS3 was a nanorod structure with 122 nm diameterand 41 nm length. The band gap was 1.31 eV and the EVB was located at 0.15 eV from the Mott-Schottky analysis. The Cu3BiS3 electrode exhibited an excellent HER performance with an onset potential of 870 mV, a Tafel slope of 251.07mV/dec. And the charge transfer resistance (Rct) of Cu3BiS3 electrode was 7.2 Ω. The electrode also revealed the goodphotoelectric response ability. The electrode also revealed the good photoelectric response ability and good stability bytime course of the photocurrents. This work provided a fast, low-cost method to prepare the Cu3BiS3 for photocatalysiswater splitting.

Gene Expression of Heart and Adipocyte Fatty Acid-binding Protein in Chickens by FQ-RT-PCR

Tu, Yunjie,Su, Yijun,Wang, Kehua,Zhang, Xueyu,Tong, Haibing,Gao, Yushi Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.8

This study was to detect the expression of heart fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) gene mRNA in different tissues of Rugao and Luyuan chickens at 56 d and 120 d by real-time fluorescence quantitative reverse transcription polymerase-chain reaction (FQ-RT-PCR). The primers were designed according to the sequences of HFABP, A-FABP and GAPDH genes in Gallus gallus, which were used as target genes and internal reference gene, respectively. The levels of H-FABP and A-FABP gene expression were detected by SYBR Green I FQ-RT-PCR. The relative H-FABP and A-FABP gene mRNA expression level was calculated with 2-$^{{\Delta}Ct}$. Melting curve analysis showed a single peak of three genes. Intramuscular fat (IMF) content in breast muscle and leg muscle of the two chicken breeds at 120 d was higher than at 56 d. IMF content in breast muscle and leg muscle at 56 d and 120 d in Luyuan was significantly higher than in Rugao, however, abdominal fat of Luyuan was significantly lower than that of Rugao. The relative H-FABP gene mRNA expression level in cardiac muscle was the highest in both chicken breeds. The relative H-FABP and A-FABP gene expression of different tissues in Luyuan was higher than in Rugao. H-FABP gene mRNA expression had a negative effect on IMF of leg and breast muscles, and was significantly negatively correlated with IMF content. The relative A-FABP gene mRNA level in abdominal fat was higher than in liver. The A-FABP gene mRNA was not expressed in leg, breast and cardiac muscles. A-FABP gene mRNA expression level was significantly positively correlated with abdominal fat and had a significant effect on abdominal fat but not IMF content.

Betulin induces reactive oxygen species-dependent apoptosis in human gastric cancer SGC7901 cells

Yang Li,Xiaokang Liu,Dan Jiang,Yingjia Lin,Yushi Wang,Qing Li,Linlin Liu,Ying-Hua Jin 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.9

Betulin, an abundant natural compound, significantly inhibited the cell viability of advanced human gastric cancer SGC7901 cells. Mechanism study demonstrated that betulin induced apoptosis through mitochondrial Bax and Bak accumulation-mediated intrinsic apoptosis pathway. Downregulation of the anti-apoptosis proteins Bcl-2 and XIAP was involved during betulin-induced cell apoptosis. Reactive oxygen species (ROS) was generated in cells after betulin treatment in a time- and dose-dependent manner. Addition of antioxidant N-acetyl-L-cysteine (NAC) significantly attenuated betulin-induced ROS generation as well as Bcl-2 and XIAP downregulation. The mitochondrial accumulation of Bax and Bak, as well as caspase activity, was also remarkably inhibited by NAC treatment, indicating that ROS are important signaling intermediates that lead to betulin-induced apoptosis by modulating multiple apoptosis-regulating proteins in SGC7901 cells.

Low Velocity Impact Behavior of Aluminum Honeycomb Structures

Yu Shi,Yi-Qi Wang,Ali Md. Afsar,Jung-Il Song,Intaek Lee,Yasuo Ochi 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

Impact behavior of Aluminum Honeycomb Composites (AHC) under the impact loading is checked by using an explicit definition of dynamic ductile failure. Applicable testing followed by drop weight test is also investigated in this study. The specimens with 1/2inches cell size are tested by two impactors with the weight of 5.25Kgf and 11.9Kgf respectively. Dynamic nonlinear transient analysis using finite element method is accomplished for nonlinear contact and elastoplastic behavior described in FE model of AHC. Impact behavior of AHC along with different contact points appeared nearly the same in low impact energy, but it was different in high impact energy. At the centered contact point of honeycomb core cell, resistance peak force shows the highest and at the contact on short-edge of the core cell, its resistance shows the lowest. Comparing fracture mode and time history of resistance force with test, real time deformation from FE results gives the fracture process when the crack initiated and propagated to go further penetration from faced sheet to the honeycomb core.

Durability Test And Crash Analysis Of Automobile Power Seat

Yu Shi,Yi-Qi Wang,Ali Md. Afsar,Jung-Il Song 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

'Turny type power seat' was developed to have disabled person get on and off a vehicle without troubles. At the same time, it is imperative to ensure the safety and stability of the power seat which necessitates various tests and analysis. In this study, the durability test and crash analysis were performed for the power seat. The durability test was performed by applying a load of 100㎏ to simulate the weight of passenger. Crash analysis was carried out by using the LS-DYNA program. The durability lest ensured a longer life cycle of the power seat. Further, the results of crash analysis showed that the distorted deformation was so low that it could not bring the danger for passengers. However, the forward displacement of the seat was much more to cause a large deformation of the hook plate, which was dangerous for passengers. Therefore, further modifications of the power seat should be made to ensure better performance, especially to reduce the forward displacement when the crash happens.