Preclinical and clinical studies on cytokine-induced killer cells for the treatment of renal cell carcinoma

Kim, Ji Sung,Chung, In Sung,Lim, Sang Hee,Park, Yunsoo,Park, Mi Jeong,Kim, Ju Young,Kim, Yong Guk,Hong, Jin Tae,Kim, Youngsoo,Han, Sang-Bae 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

Renal cell carcinoma (RCC) is the most common malignancy of adult human kidney, which accounts for more than 2 % of all cancers. RCC generally does not respond well to conventional chemotherapy and radiotherapy. Cytokine-induced killer (CIK) cells are ex vivo activated lymphocytes with potent activity against various tumors and minimal side effects. Here, we summarize the data on preclinical and clinical efficacy of CIK cells for RCC treatment. Our preclinical data show that CIK cells have potent anti-tumor activity in vitro and in an in vivo nude mouse xenograft model. Clinical studies for the treatment of RCC patients indicate that CIK cell therapy can induce favorable responses with no serious side effects. These studies suggest that CIK cells may become a valuable strategy for the treatment of patients with RCC.

대규모유통업법의 적용 범위에 관한 고찰 - 대규모유통업법의 입법 취지를 중심으로 -

김정헌 ( Kim Junghun ),박종하 ( Park Jongha ),김규식 ( Kim Kyusik ),김영석 ( Kim Youngseok ),김윤수 ( Kim Yunsoo ) 한국유통법학회 2020 유통법연구 Vol.7 No.2

대규모유통업법은 공정거래법상 거래상 지위 남용행위에 대한 특별법임을 감안하여 대규모유통업자가 거래상 우월적 지위를 가지지 않는 거래에 대하여는 대규모유통업법을 적용하지 않도록 규정하였다. 그런데, 최근 공정거래위원회 및 법원은 대규모유통업법상 대규모유통업자에 해당하기만 하면 실질적으로 납품업자에 대하여 거래상 우월적 지위를 가지고 있는지에 대한 심도 있는 검토 없이 그대로 대규모유통업법을 적용하는 경향을 보이고 있다. 그러나, 대규모유통업법이 대규모유통업자와 납품업자 간의 사법상의 권리관계에 제한을 가하는 것으로서 본질적으로 사적 자치의 한계 내지 예외에 해당한다는 점, 공정거래법상 거래상 지위 남용행위의 특별법으로서 강력한 규제가 부과된다는 점 등에 비추어보면, 그 적용 범위를 엄격하게 해석 및 운용할 필요가 있다. 현실적으로 규제당국이 각 납품업자와 대규모유통업자간의 거래상 지위를 일일이 판단하는 것이 어렵다면, 일정한 매출액 규모 요건 등 보다 객관적인 기준을 통해 누가 보더라도 대규모유통업법 적용 대상인지 여부를 쉽게 판단할 수 있도록 개선할 필요가 있다. 한편, 대규모유통업법은 일정 규모 이상의 가맹본부에 대해서도 대규모유통업자로 간주하고 있다. 그러나, (ⅰ) 대규모유통업법은 기본적으로 백화점, 대형마트 등 거래상 지위를 가진 소매업자의 중소 납품업자나 임차인에 대한 불공정거래행위를 규제하는 것을 그 목적으로 하는 법률이어서 도매업자에 가까운 가맹본부에 대해서까지 적용하는 것이 타당한지에 대한 의문이 있다는 점, (ⅱ) 가맹본부에 대규모 유통업법을 적용함으로써 가맹본부로 집결된 협상력을 제한한 결과, 공동구매에 따른 협상력 제고라는 가맹사업의 본질을 해하고 결과적으로 가맹점사업자 보호에 역행한다는 점, (ⅲ) 특히 편의점 가맹본부의 경우 전체 구매 상품 중 67~90%에 이르는 상품을 대기업으로부터 구매하는바, 대규모유통업법으로 인하여 영세한 가맹점사업자보다 오히려 대기업인 납품업자를 보호하게 되는 불합리한 결과가 초래 된다는 점 등에 비추어보면, 관련 규정을 개정할 필요성이 인정된다. 즉, 가맹본부를 대규모유통업법 적용 대상인 대규모유통업자의 범위에서 제외하는 방안을 적극 검토할 필요가 있다. 아울러, 최근 대규모유통업법 개정으로 인해 유통업자가 아닌 부동산 임대사업자에 대하여도 대규모유통업법이 적용되게 되었는바, 이는 대규모유통업자를 규제하는 대규모유통업법의 취지에 부합하지 않고, 상가임대차법과 중복된 이중적 규제라는 등의 문제점이 있으므로 이러한 규제를 유지할 것인지에 대하여도 신중한 검토가 필요하다고 생각된다. The Act on Fair Transactions in Large Retail Business (hereinafter the Large Retail Act) shall not apply to a transaction which is not deemed to give a large retailer a superior bargaining position over a supplier (Article. 3.1.). Nonetheless, recently, the Korea Fair Trade Commission and courts tend to apply the Large Retail Act directly to a certain retail business entity without an in-depth analysis of whether it has actually superior bargaining position over a supplier as long as it is a large retailer under the Large Retail Act However, considering that the Large Retail Act is essentially an exception to principle of private autonomy and it substantially regulates the business activities of large retailer, it is necessary to narrowly interpret the scope of a large retailer. In particular, the Large Retail Act stipulates that the franchisers of a certain size or larger also fall within the scope of a large retailer. However, considering the following, it is highly recom- mended to revise the relevant regulations;(ⅰ) the purpose of the Large Retail Act is basically to regulate the abuse of market dominance or unfair trade practices by large retailers, such as department stores and supermarket. (ⅱ) If the bargaining power aggregated into the franchiser is limited by applying the Large Retail Act to the franchiser, the nature of the franchise business, which is enhancing franchisee's bargaining power by purchasing jointly will be undermined and consequently the protection of franchisees will be impaired;(ⅲ) especially, since convenience store franchisers purchase 67 to 90 percent of the total purchased goods from large companies, the application of the Large Retail Act to franchiser results in unfairly protecting the large suppliers more than the franchisees, which are much smaller than the large suppliers. In order to solve these problems, it is desirable to establish sales volume requirements to determine whether to apply the Large Retail Act, and furthermore, exclude the franchiser from the scope of a large retailer which is subject to the Large Retail Act. Meanwhile, due to the recent revision, the Large Retail Act also applies to real estate rental businesses, which is not distributor. This is not consistent with the objective of the Large Retail Act, which regulates a large retailer, and causes overlapping regulations between the Commercial Building Lease Protection Act and the Large Retail Act. Therefore, a careful consideration is also required on whether to maintain this regulatory framework.

MOCVD and Characterization of ZnO Thin Films Using and Aminoalkoxide Single Precursor

Ju Yeon Kim,Min Chan Kim,Young Kuk Lee,Taek-Mo Chung,Chang Gyoun Kim,Yunsoo Kim 한국진공학회 2004 한국진공학회 학술발표회초록집 Vol.- No.-

약용작물 잔대와 천궁 중 Pyraclostrobin의 잔류특성

서정아 ( Jeong-a Seo ),김태한 ( Taehan Kim ),박홍묵 ( Hongmuk Park ),이윤수 ( Yunsoo Lee ),김준희 ( Junhui Kim ),박인호 ( Inho Park ),김영대 ( Youngdae Kim ),김주환 ( Juhwan Kim ),김동현 ( Donghyun Kim ),맹지현 ( Jihyun Maeng ),김지 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-

본 연구는 pyraclostrobin의 잔대 및 천궁에 대한 잔류성을 구명하여 잔류농약의 잔류특성 및 안정성평가를 위한 기초자료로 활용코자 하였다. Pyraclostrobin은 strobilurin계로 식물병원균의 포자발아억제, 균사생육저지의 예방효과가 우수하며, 예방 및 치료용의 침투이행성 살균제로 사용되고 있다. 잔대의 시험 약제는 디메토모르프.피라클로스트로빈 25.5(16+9.5)% 액상수화제를 2,000배 희석하여 3회 살포 후 7일, 14일, 21일, 30일 경과후 수확하였으며, 천궁의 시험약제는 피라클로스트로빈 20% 입상수화제를 3,000배 희석하여 3회 살포 후 7일, 14일, 21일, 30일 경과후 수확하였다. Pyraclostrobin은 acetonitrile로 추출하고 응용된 QuEChERS 분석법을 이용하여 LC/MS/MSD로 기기분석하였으며, 분석법상 정량한계(method limit of quantitation; MLOQ)는 모두 0.01 mg/kg이었다. 분석의 정확성 및 재현성 검증을 위하여 약제를 처리하지 않은 공시료에 정량한계, 정량한계 10배 수준이 되도록 표준용액을 첨가하여 3반복 회수율 실험을 실시하였다. 회수율 시험 결과는 농촌진흥청에서 고시한 회수율의 유효 범위 및 변이계수(coefficient of variation, C.V.)를 만족하였다. Pyraclostrobin의 잔류량은 건잔대에서 정량한계(0.01 mg/kg) 미만∼0.07 mg/kg, 생잔대에서 정량한계(0.01 mg/kg) 미만∼0.01 mg/kg이었고, 건천궁의 잔류량은 0.21 mg/kg∼2.18 mg/kg, 생천궁 잔류량은 0.08 mg/kg∼0.14 mg/kg이었다. 생시료를 건시료로 건조하였을 때 잔대의 건조수율은 평균 22.8%, 천궁은 40.6%로 였다. 시험 작물 중 건조 농산물 잔류량이 생 농산물에 비해 높게 잔류되었으며, 건조 과정 중 수분제거로 인한 농약잔류량의 증가로 판단되었다.

지속가능한 정화공법의 평가와 관리

Yunsoo Kim,Jae-Woo Park(박재우) 한국지반환경공학회 2013 한국지반환경공학회 학술발표회논문집 Vol.2013 No.9

Journal statistics in 2023 and catalysts for the future transformation of Child Health Nursing Research

Yunsoo Kim 한국아동간호학회 2024 Child Health Nursing Research Vol.30 No.1

Primary Hyperoxaluria in Korean Pediatric Patients

Yunsoo Choe,이지원,Ji Hyun Kim,Myung Hyun Cho,Seong Heon Kim,Joo Hoon Lee,Young Seo Park,Hee Gyung Kang,Il Soo Ha,Hae Il Cheong 대한소아신장학회 2019 Childhood kidney diseases Vol.23 No.2

Background: Primary hyperoxaluria (PH), a rare inborn error of glyoxylate meta bolism causing overproduction of oxalate, is classified into three genetic subgroups: type 1–3 (PH1–PH3) caused by AGXT, GRHPR , and HOGA1 gene mutations, respectively. We performed a retrospective case series study of Korean pediatric patients with PH. Methods: In total, 11 unrelated pediatric patients were recruited and their phenotypes and genotypes were analyzed by a retrospective review of their medical records. Results: Mutational analyses revealed biallelic AGXT mutations (PH1) in nine patients and a single heterozygous GRHPR and HOGA1 mutation in one patient each. The c.33dupC was the most common AGXT mutation with an allelic frequency of 44%. The median age of onset was 3 months (range, 2 months–3 years), and eight patients with PH1 presented with end stage renal disease (ESRD). Patients with two truncating mutations showed an earlier age of onset and more frequent retinal involvement than patients with one truncating mutation. Among eight PH1 patients presenting with ESRD, five patients were treated with intensive dialysis followed by liver transplantation (n=5) with/without subsequent kidney transplantation (n=3). Conclusion: Most patients presented with severe infantile forms of PH. Patients with two truncating mutations displayed more severe phenotypes than those of patients with one truncating mutation. Sequential liver and kidney transplantation was adopted for PH1 patients presenting with ESRD. A larger nation-wide multicenter study is needed to confirm the genotype-phenotype correlations and outcomes of organ transplantation.

Heteroepitaxial Growth of Silicon Carbide Thin Films by High Vacuum Chemical Vapor Deposition Using the Single Precursor 1,3 - Disilabutane

Yunsoo Kim 한국진공학회 1996 한국진공학회 학술발표회초록집 Vol.- No.-

Determination of lipophilic metabolites for species discrimination and quality assessment of nine leafy vegetables

Kim, Tae Jin,Lee, Kyoung Bok,Baek, Seung-A,Choi, Jaehyuk,Ha, Sun-Hwa,Lim, Sun-Hyung,Park, Soo-Yun,Yeo, Yunsoo,Park, Sang Un,Kim, Jae Kwang The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

The lipophilic compounds in nine vegetables consumed in Korea were characterized for diversity in phytochemical content. We also analyzed the relationships among these compounds in terms of their contents. The profiles of 18 lipophilic compounds in the leaves were subjected to data-mining processes, including principal component analysis (PCA), Pearson's correlation analysis, hierarchical clustering analysis (HCA), and partial least squares discriminant analysis (PLS-DA). These species could be distinguished by means of the PCA results. HCA of these phytochemicals resulted in clusters derived from closely related biochemical pathways. PLS-DA showed significant separation among extracts from the following four families: Amaranthaceae, Asteraceae, Brassicaceae, and Malvaceae. The major metabolites that facilitated differentiation in the PLS-DA model were campesterol, ${\beta}$-sitosterol, ${\beta}$-amyrin, stigmasterol, cholesterol, octacosanol (c28), ${\alpha}$-amyrin, and hexacosanol (c26). Chard contained high levels of triacontanol (c30), which was positively correlated with the c28 content (r = 0.746, p < 0.0001). Stigmasterol, ${\alpha}$-amyrin, and ${\beta}$-amyrin contents were higher in Asteraceae species including chicon, lettuce, and oak than other plant families. These results demonstrate the utility of metabolic profiling, combined with multivariate analysis, for discrimination of vegetable species as well as evaluation of food quality.

Chemical Vapor Deposition with Single Precursors

Yunsoo Kim 한국진공학회 1991 한국진공학회 학술발표회초록집 Vol.- No.-