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RISS 인기검색어
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- 저자 : Yujun Luo (검색결과 4 건)
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Study of Hybrid DNA Physical Mapping Based on Approximation Algorithm with Errors
Zhenglong Liu,Yanmei Yang,Yujun Luo,Hongping Wang 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.2
A human chromosome is a DNA molecule with approximately 108 base pairs. The techniques developed to date for sequencing are restricted to pieces of DNA with up to tens of thousands of base pairs. This means that when a piece is sequenced, only an extremely small part of a chromosome can be seen. Molecular biologists use special techniques to deal with DNA molecules comparable in size to a chromosome. These techniques enable them to create maps of an entire chromosome or of significant fractions of chromosomes. Computational techniques were studied that could potentially aid biologists in the map-generation process. An algorithm that solves the consecutive 1s problem was studied. Such a problem is a good model of hybridization mapping when there are no errors and when probes are unique. If errors are present, another approach is needed, and the approximation algorithm is a prospective problem solver for hybridization physical mapping of DNA with errors.
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Li Ping,Zou Liuyi,Luo Zuojun,Lu Yuhua,Yu Shuang,Zhu Yujun,Xie Yong 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3
Background Non-small cell lung cancer (NSCLC) is one of most threatening malignancies with a high morbidity and mortality that threaten human health and life. Objective This study aimed to investigate the role of circBLNK in NSCLC and reveal the regulation mechanism of circBLNK in NSCLC. Quantitative real-time polymerase chain reaction ( qRT-PCR) was performed to determine the levels of circBLNK, miR-578 and inhibitor of growth 5 (ING5) mRNA. Cell proliferation activity was assessed by 3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyl-2-H-tetrazolium bromide (MTT), 5-ethynyl-2’-deoxyuridine (EdU) staining and colony formation assays. Flow cytometry was carried out to examine cell cycle and cell apoptosis. The dual-luciferase reporter assay was used to validate the interaction between miR-578 and circBLNK or ING5. Xenograft tumor experiment was performed to uncover the function of circBLNK in vivo. Results CircBLNK was notably down-regulated in NSCLC tissues and cells. Overexpression of circBLNK suppressed the proliferation and accelerated the apoptosis of NSCLC cells in vitro . CircBLNK targeted miR-578, and circBLNK exerted its biological function in NSCLC cells through sponging miR-578. ING5 was verifi ed as a target of miR-578, and circBLNK increased the abundance of ING5 through targeting miR-578 in NSCLC cells. ING5 interference could partly reverse the biological eff ects of NSCLC cells mediated by circBLNK overexpression. CircBLNK overexpression repressed NSCLC tumor growth in vivo. Conclusion CircBLNK functioned as a tumor suppressor in NSCLC to suppress the proliferation and cell cycle and promote cell apoptosis of NSCLC cells through miR-578/ING5 axis.
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Shuai Hao,Wuguo Tian,Jianjie Zhao,Yi Chen,Xiaohua Zhang,Bo Gao,Yujun He,Donglin Luo 한국유방암학회 2020 Journal of breast cancer Vol.23 No.4
Purpose: Real-time detection and intervention can be used as potential measures to markedly decrease breast cancer mortality. Assessment of circulating tumor DNA (ctDNA) may offer great benefits for the management of breast cancer over time. However, the use of ctDNA to predict the effectiveness of neoadjuvant treatment and recurrence of breast cancer has rarely been studied. Methods: We prospectively recruited 31 breast cancer patients with 4 subtypes. Three time points were set in this study, including before any therapy (C1), during surgery (T), and six months after surgery (C2). We collected peripheral blood samples from all 31 patients at C1, tumor tissue from all 31 patients at T, and peripheral blood samples from 25 patients at C2. Targeted 727-gene panel sequencing was performed on ctDNA from all blood samples and tissue DNA from all tissue samples. Somatic mutations were detected and analyzed using a reference standard pipeline. Statistical analysis was performed to identify possible associations between ctDNA profiles and clinical outcomes. Results: In total, we detected 159, 271, and 70 somatic mutations in 30 C1 samples, 31 T samples, and 12 C2 samples, respectively. We identified specific genes, such as PIK3CA, TP53, and KMT2C, which were highly mutated in the tissue samples. Furthermore, mutated KMT2C observed in ctDNA of the C2 samples may be an indicator of breast cancer recurrence. Conclusion: Our study highlights the potential of ctDNA analysis at different timepoints for assessing tumor progression and treatment effectiveness, as well as prediction of breast cancer recurrence.
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On Which Microphysical Time Scales to Use in Studies of Entrainment‐Mixing Mechanisms in Clouds
Lu, Chunsong,Liu, Yangang,Zhu, Bin,Yum, Seong Soo,Krueger, Steven K.,Qiu, Yujun,Niu, Shengjie,Luo, Shi American Geophysical Union 2018 Journal of Geophysical Research: Atmospheres Vol.123 No.7
<P>The commonly used time scales in entrainment-mixing studies are examined to seek the most appropriate one, based on aircraft observations of cumulus clouds from the RACORO campaign and numerical simulations with the Explicit Mixing Parcel Model. The time scales include the following: (evap), the time for droplet complete evaporation; (phase), the time for saturation ratio deficit (S) to reach 1/e of its initial value; (satu), the time for S to reach -0.5%; and (react), the time for complete droplet evaporation or S to reach -0.5%. It is found that the proper time scale to use depends on the specific objectives of entrainment-mixing studies. First, if the focus is on the variations of liquid water content (LWC) and S, then (react) for saturation, (satu) and (phase) are almost equivalently appropriate, because they all represent the rate of dry air reaching saturation or of LWC decrease. Second, if one focuses on the variations of droplet size and number concentration, (react) for complete evaporation and (evap) are proper because they characterize how fast droplets evaporate and whether number concentration decreases. Moreover, (react) for complete evaporation and (evap) are always positively correlated with homogeneous mixing degree (); thus, the two time scales, especially (evap), are recommended for developing parameterizations. However, and the other time scales can be negatively, positively, or not correlated, depending on the dominant factors of the entrained air (i.e., relative humidity or aerosols). Third, all time scales are proportional to each other under certain microphysical and thermodynamic conditions.</P>
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