DNA methylation comparison between 4-day-old queen and worker larvae of honey bee

Yuanyuan Shi,Hao Liu,Ya Feng Qiu,Zhi Yong Ma,Zhijiang Zeng 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

The honey bee is a social insect that is famous for queen-worker differentiation. Numerous studies indicate that queen larvae (QL) and worker larvae (WL) have different expressed genes and proteins. DNA methylation has been found to play an important role in regulating gene expression. To further explore the roles of the methylated genes in queen-worker differentiation, we analyzed DNA methylome profiles of 4-day-old QL and WL (Apis mellifera). The results demonstrated that therewere 7.2 gigabases of sequence data fromsix methylated DNA immunoprecipitation libraries, and provided a genome-wide DNA methylation map as well as a gene expression map for 4-day-old QL andWL. The genome coverage of every samplewas 4.79. According to CpG representation, all promoters in the A. mellifera genome were classified into high CpG promoters, intermediate CpG promoters and low CpG promoters. The methylated cytosines of larvae were enriched in introns, followed by coding sequence regions, 2 K downstream of genes, 5′ untranslated regions (UTRs), 2 K upstream of genes, and 3′ UTRs. Compared with 4-day-oldWL, a number of genes in QL were down-methylated that were involved in biological regulation, immune system and metabolic regulation. In addition, these DMGs were involved in many signal pathways of caste differentiation such as Mitogen-activated protein kinase (MAPK), Notch, Insulin andWnt signaling pathways.

A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi

Yuanyuan Chen,Bernard A. Prior,Guiyang Shi,Zhengxiang Wang 한국미생물학회 2011 The journal of microbiology Vol.49 No.4

In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.

Distribution of Soil Textures in Chinese Flue-Cured Tobacco Growing Regions and its Relationship with Tobacco Quality and Style

Hongzhi Shi,Yingli Song,Yuanyuan Yang 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

An Improved Anonymous Remote user Authentication Scheme with Key Agreement based on Dynamic Identity

Yajuan Shi,Han Shen,Yuanyuan Zhang,Jianhua Chen 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.5

To keep the pace with the development of internet technology, remote user authentication techniques become more and more important to protect user’s privacy. Recently, Kumari, et al., presented an improved remote user authentication scheme with key agreement based on dynamic-identity using smart card. This scheme allows legal users to change the password at his will without the need to connect the server. They claimed that their scheme could resist smart card stolen or loss attack, user impersonation and server masquerading attack, and provide user anonymity and untraceability and so on. However, our research indicates that their scheme is completely unsafe. Furthermore, the scheme can’t provide the proper mutual authentication. In this manuscript, we will propose a new scheme, which can withstand those attacks mentioned above and provide the perfect user anonymity and forward secrecy. Security analysis makes it clear that the improved scheme apparently is more secure and practical.

Deposition of Nd-Doped Fe2O3 Nanoparticles on Cenosphere by Hydrothermal Method

Hui Zhang,Yuanyuan Shi,Jun Xu,Runjun Sun 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.3

A layer of flake-like Fe2O3 particles doped with rare earth Nd3+ ions is homogeneously coated on the surface of cenosphere by using ferric nitrate as the iron source, tartaric acid as the precipitating agent, hexadecyl trimethyl ammonium bromide as the dispersing agent and neodymium nitrate as the dopant via a facile hydrothermal route. The as-prepared cenosphere is characterized by various analytical techniques such as field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, transmission electron microscopy, vibrating sample magnetometry, thermal gravimetric analysis, differential scanning calorimetry, diffuse reflectance spectroscopy, photoluminescence spectroscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The performances of photocatalytic degradation of methylene blue dye are also investigated under ultraviolet and visible light irradiations. The experimental results indicate that the doping concentration of Nd3+ ions is optimized as 0.4% with respect to Fe3+ ions, and the rare earth Nd3+ ions are highly dispersed onto Fe2O3 particle surface. After being doped with Nd3+ ions, the photoactivity of the 0.4% Nd-doped Fe2O3 coated cenosphere is distinctly improved. The magnetic properties are also enhanced to a large extent.

Analysis of the Transcriptome of Polygonatum odoratum (Mill.) Druce Uncovers Putative Genes Involved in Isofavonoid Biosynthesis

Shengxiang Zhang,Yuanyuan Shi,Chunmiao Shan,Liqiang Zhao,Kelong Ma,Luqi Huang,Jiawen Wu 한국식물학회 2020 Journal of Plant Biology Vol.63 No.3

Polygonatum odoratum (Mill.) Druce (P. odoratum) is a traditional Chinese herb. The isofavonoids of P. odoratum are important medicinal bioactive compounds for the treatment of hypoimmunity, rheumatic heart disease, cardiovascular diseases, and diabetes. This study used RNA sequencing to identify potential genes regulating isofavonoid biosynthesis in P. odoratum. To do this, we generated an overview of metabolic and secondary metabolic pathways, using MAPMAN, and identifed 96 genes encoding seven key enzymes involved in isofavonoid biosynthesis through their KEGG annotation. Alignment of phenylalanine ammonia-lyase or chalcone synthase amino acid sequences revealed well-conserved sequences, spatial structures, and active sites. We also verifed the expression of key genes encoding phenylalanine ammonia-lyase and chalcone synthase by quantitative real-time PCR (qRT-PCR). This analysis of the isofavonoid biosynthesis pathway and its crucial enzymes in P. odoratum lays the foundation for uncovering the regulatory mechanism of isofavonoid biosynthesis.

High-Fat Diets Impair Spatial Learning of Mice in the Y-Maze Paradigm: Ameliorative Potential of α-Lipoic Acid

Yonghua Cui,Yuxin Shu,Yuanyuan Zhu,Yonghui Shi,Guowei Le 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.8

High-fat diets (HFDs) have been found to influence central nervous system development and to cause cognitive impairments in human epidemiologic studies, as well as in animal investigations. These adverse effects on learning and memory induced by an HFD have been associated with an impaired hippocampus, including hippocampal oxidative damage. Previously, we had found that a-lipoic acid (a-LA) could ameliorate the oxidative stress in non-neural organs (liver, jejunum,and spleen) induced by a 10-week HFD (21.2% fat) food regimen in mice. In this study, we investigated whether a 10-week HFD (21.2% fat) induced oxidative stress in the hippocampus or impaired spatial learning in mice and whether LA ameliorated these effects. The HFD was found to induce oxidative stress (a decrease in catalase activity, glutathione peroxidase activity, and total antioxidative capacity and an increase in malondialdehyde levels) in the mouse hippocampus. In addition,we found that the HFD impaired spatial recognition memory of mice in the Y-maze paradigm. Furthermore, the hippocampal oxidative stress and impaired spatial recognition memory of the mice were reduced in HFD diets supplemented with 0.1% LA. These findings suggest that LA, as a strong antioxidant, may help prevent HFD-induced learning impairments by ameliorating associated oxidative stress in the hippocampus.

Synergistic catalysis of bifunctional polyacrylonitrile fiber for the synthesis of (E)-α,β-unsaturated esters from aldehydes by decarboxylative Doebner–Knoevenagel reaction

Pengyu Li,Liwei Mi,Yuanyuan Liu,Wenqin Zhang,Xian-Lei Shi 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.81 No.-

A series of monofunctional and bifunctional polyacrylonitrilefiber catalysts have been successfullyprepared to catalyze the decarboxylative Doebner–Knoevenagel reaction of aldehydes and monoethylmalonate for the syntheses of the (E)-α,β-unsaturated esters. Among which, the catalyst PANPDFII/I showsthe best synergistic catalytic activity with high yields (89–96%), stereoselectivities (Z:E > 99:1) andreusability (up to 8 times). The influences of the N position on aminopyridine moiety, solvent andtemperature on the catalytic system were investigated in detail. This catalytic system can process well inhigher or lower polar organic solvents instead of moderate polar solvents, which is an interestingphenomenon in organic catalysis, and the reasons are explained in this work. Furthermore, a DMAP (4-dimethylaminopyridine) and piperazine synergistic catalytic mechanism in the micro-environment ofthefiber catalyst has been conceived to explain the high catalytic performance of this catalytic system. Besides, thefiber catalyst PANPDFII/I shows high application potential in industry for its good performancein scaled-up experiment, as well as its advantages of the easy preparation, high mechanical strength,flexibility and high stability in air.

Modulation electronic structure of NiS nanoarray induced by Fe, V doping for high efficiency water and urea electrolysis

Xiaojuan Feng,Yanlong Shi,yuanyuan Chen,Zhirong Xu,Haiyu Guan 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

Exploring high-efficient and stable low-cost electrocatalysts is of significant importance for boosting theefficiency of water splitting and purifying urea-enriched wastewater. Herein, bimetallic doping strategywas adopted to obtain jasminum nudiflorum-like Fe, V doped NiS arrays (Fe, V-NiS/NF) via typicalhydrothermal process and subsequent anion exchange reaction. The as-obtained Fe, V-NiS/NF array displayshigh catalytic activity and stability toward oxygen evolution reaction (OER) and urea oxidationreaction (UOR) in alkaline media, with reduced overpotentials of 273 and 214 mV to deliver the currentdensity of 50 mA cm2 for OER and UOR, respectively. More notably, when employing Fe, V-NiS/NF assymmetric electrolytic cell for urea electrolysis, a low cell voltage of 1.45 V is needed at 10 mA cm2,which is about 110 mV lower than the conventional water electrolysis. Meanwhile, the catalyst also displayssuperior stability for over 72 h. Such outstanding performance is attributed to the following points:(i) 3D porous flower-like structure facilitates the mass transfer and abundant exposure of active sites; (ii)in situ growth of catalysts on conductive substrate and the effective interface engineering of differentcomposition shorten the charge transport pathways and expedite electron transfer. Density functionaltheory calculations demonstrate that the Fe and V dopants regulate the electronic environment of Ni sitesand optimize the adsorption free energy of urea. This work provides a universal pathway to design highefficientand non-noble electrocatalysts for H2 production in an energy-saving way via urea electrolysis.

Effects of three drying methods on polyphenol composition and antioxidant activities of Litchi chinensis Sonn.

Si Tan,Jianmin Tang,Wenjing Shi,Zhuwei Wang,Yuanyuan Xiang,Tingwei Deng,Xiaoxu Gao,Wenfeng Li,Shengyou Shi 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.3

The aim of this study was to investigate the effects of three different drying methods, freeze drying (FD), vacuum drying (VD) and oven drying (OD) on phenolic contents and antioxidant activities of litchi fruits. 20 polyphenols were exactly identified in the litchi fruits by UPLC-QqQ/MS. Significant losses were observed in the contents of total polyphenols and antioxidant activities in the dried litchi when compared with the fresh litchi. Principle component analysis indicated that there was significant difference of phenolic component between the use of thermal drying (VD and OD) and FD. Our results suggest that FD is the optimum drying method for litchi fruits considering the content of total polyphenols and antioxidant activities.