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RISS 인기검색어
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- 저자 : Guiyang Shi (검색결과 3 건)
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A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi
Yuanyuan Chen,Bernard A. Prior,Guiyang Shi,Zhengxiang Wang 한국미생물학회 2011 The journal of microbiology Vol.49 No.4
In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.
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Liang Zhang,Zhongyang Ding,Peng Xu,Yuhong Wang,Zhenghua Gu,Zhu Qian,Guiyang Shi,Kechang Zhang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3
Tyrosinase is a key enzyme in the biosynthesis of melanin, and the use of inhibitors against tyrosinase can prevent hyperpigmentation by inhibiting enzymatic oxidation. However, the current use of tyrosine inhibitors is limited by their low activities and high toxicities. The aim of the present research was to develop novel whitening agents, or tyrosinase-targeted medicine, from a submerged culture of the fungus Ganoderma lucidum. Methyl lucidenate F was isolated from the ethanol-soluble-acidic components (ESACs) of G. lucidum, with the structure of ESACs elucidated via UV, LC-MS, and ^(13)C-NMR spectral analysis. The tyrosinase inhibitory activity was measured using catechol as a substrate. Methyl lucidenate F displayed uncompetitive inhibition of the potato tyrosinase activity,for which Lineweaver-Burk plots revealed a maximum reaction rate (V_(max)) of 0.4367/min, Michaelis constant (K_m)of 6.765 mM and uncompetitive inhibition constant (K_i) of 19.22 μM. Meanwhile, methyl lucidenate F (tetra cyclic triterpenoid) exhibited high tyrosinase inhibitory activity,with an IC_(50) of 32.23 μM. These results suggest that methyl lucidenate F may serve as a potential candidate for skin-whitening agents.
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Application of full permeate recycling to very high gravity ethanol fermentation from corn
Zhongyang Ding,Liang Zhang,Yaye Fang,Kechang Zhang,GuiYang Shi,Ling Xu 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3
A ceramic membrane with pore size of 0.2 μm was used to percolate grain stillage of very high gravity (VHG) ethanol fermentation from corn, and the micro-filtration permeate was completely recycled for the cooking step in the next fermentation process. The concentrations of solids, sugars, total nitrogen and Na+ in the grain stillage and permeate reached a relative steady state after two or three batches of filtration and recycling process. There are no negative effects of by-products on VHG ethanol fermentation, and the final ethanol yield was above 15% (v/v). The conditions of filtration were examined to determine the optimum conditions for the process and included an initial flux of clean water above 550 L·m−2·h−1 (0.1 MPa), an operating differential pressure of 0.15 MPa, an operating temperature above 70℃, and a permeation flux greater than 136 L·m−2·h−1. It could be concluded that full permeate recycling during ethanol production was an efficient process that resulted in less pollution and less energy consumption.
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