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Youngcho Cho,Young-Pyo Lee,Sunggil Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Cytoplasmic male sterility caused by DCGMS (Dongbu cytoplasmic and genic male-sterility) cytoplasm and its nuclear restorer-of-fertility locus (Rfd1) with a linked molecular marker (A137) have been reported in radish (Raphanus sativus L.). To construct a linkage map of the Rfd1 locus, linked amplified fragment length polymorphism (AFLP) markers were screened using bulked segregant analysis. A 220-bp linked AFLP fragment sequence from radish showed homology with an Arabidopsis coding sequence. Using this Arabidopsis gene sequence, a simple PCR marker (A220) was developed. The A137 and A220 markers flanked the Rfd1 locus. Two homologous Arabidopsis genes with both marker sequences were positioned on Arabidopsis chromosome 3 with an interval of 2.4 Mb. To integrate the Rfd1 locus into a previously reported expressed sequence tag (EST)-simple sequence repeat (SSR) linkage map, the radish EST sequences located in three syntenic blocks within the 2.4-Mb interval were used to develop single nucleotide polymorphism (SNP) markers for tagging each block. The SNP marker in linkage group 2 co-segregated with male fertility in an F2 population. Using radish ESTs positioned in linkage group 2, five intron length polymorphism (ILP) markers and one cleaved amplified polymorphic sequence (CAPS) marker were developed and used to construct a linkage map of the Rfd1 locus. Two closely-linked markers delimited the Rfd1 locus within a 985-kb interval of Arabidopsis chromosome 3. Synteny between the radish and Arabidopsis genomes in the 985-kbp interval were used to develop three ILP and three CAPS markers. Two ILP markers further delimited the Rfd1 locus to a 220-kb interval of Arabidopsis chromosome 3.
( Bongju Kim ),( Youngcho Cho ),( Sunggil Kim ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.1
To introduce downy mildew resistance from a yellow-colored resistant cultivar, `Santero`, into a yellow breeding line, OT803, the F<sub>1</sub> hybrid was produced by crossing Santero and OT803. The bulb color of the F1 hybrids became light pink, suggesting involvement of complementation between the DFR-A and ANS genes in the onion anthocyanin biosynthesis pathway. Since Santero contained active DFR-A and inactive ANS alleles, OT803 was assumed to harbor active ANS and inactive DFR-A alleles. However, some yellow-colored individuals of OT803 were shown to contain the homozygous genotype of the active DFR-A<sup>R4</sup>-like allele. Sequencing of 4,830-bp full-length sequences of this DFR-A<sup>R4</sup>-like allele revealed that the nucleotide sequences of the DFR-A<sup>R4</sup> and DFR-A<sup>R4</sup>-like alleles were identical except for a single nucleotide deletion in the last exon. This single base-pair deletion resulted in creation of a premature stop codon at 2-bp downstream of the deletion mutation. This new DFR-A mutant allele was designated DFR-A<sup>PS2</sup>. The RT-PCR results showed that transcripts of the DFR-A<sup>PS2</sup> allele were significantly reduced, suggesting involvement of nonsense-mediated mRNA decay (NMD) mechanism. The systematic process consisting of PCR amplification and sequencing of the PCR products was modified to identify the DFR-A<sup>PS2</sup> allele among 16 different DFR-A alleles. No additional accession was found to contain the DFR-A<sup>PS2</sup> allele from 155 diverse onion germplasm, indicating very limited distribution of this new DFR-A<sup>PS2</sup> allele.
Hyunchol Shin,Youngcho Kim IEEE 2010 IEEE Transactions on Circuits and Systems II: Expr Vol.57 No.2
<P>An active resistor-capacitor low-pass filter with simultaneously programmable high-end and low-end cutoff frequencies is presented for IEEE 802.22 cognitive radio transmitter applications. Transfer function analysis shows that the integrator frequency of a dc offset cancellation block should be tuned inversely proportional to the open-loop gain to maintain the low-end cutoff frequency at a constant value. Realized in a 0.18- ¿m complementary metal-oxide-semiconductor, the low-end cutoff frequency is successfully tuned between 740 Hz and 10 kHz over the 30-dB gain variation. Measured results show that the gain is tuned from -16.3 to +13.9 dB, and the high-end cutoff frequency is tuned from 2.1 to 6.04 MHz, while drawing 4.5 mA from a 1.8-V supply.</P>
HyungTae Jung,SeungHwan Lee,JongNam Kim,KangJoon Lee,YoungCho Chung 대한신경정신의학회 2007 PSYCHIATRY INVESTIGATION Vol.4 No.1
Objective-The EEG abnormalities of Alzheimer’s disease (AD) patients are characterized by increased slow wave activities and reduced asymmetry between the two hemispheres. We attempted to find the specific spatio-temporal EEG pattern of AD through quantitative EEG (qEEG) and the source localization of specific frequency bands. Methods-The AD group consisted of 22 patients who fulfilled the DSM-IV criteria of AD with no space occupying lesions confirmed by brain CT or MRI. The normal control (NC) group consisted of 27 subjects with no personal history of psychiatric or neurological abnormalities. We performed qEEG, compared the hemispheric asymmetry between the AD and NC groups, and tried to obtain source imaging of each frequency band using low resolution electromagnetic tomography (LORETA). Results-Compared with the NC group, the AD patients had significantly increased slow wave activities of the theta (4-7 Hz) and delta waves (1-3 Hz) over all of the electrodes. There was no statistically significant asymmetric difference between the AD and NC groups. The slow waves of the AD patients were dominant in the right hemisphere compared to the NC subjects. Increased theta wave activity was observed, especially in Brodmann area 40 (inferior parietal lobule) in the AD patients compared with the NC subjects. Increased delta wave activity was observed especially in Brodmann area 7 (postcentral gyrus) in the AD patients compared with the NC subjects. The MMSE score had a significant negative correlation with the theta waves and a positive correlation with the alpha waves in the AD patients. There was a positive correlation between the duration of illness and the theta waves in the AD patients. Conclusions-Our results showed that AD patients had increased theta and delta wave activity in the right parietal areas, which reflect their decreased brain function in these regions. Our results suggest that qEEG and LORETA imaging are useful tools for detecting and evaluating AD.