http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Youngcho Cho,Young-Pyo Lee,Sunggil Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Cytoplasmic male sterility caused by DCGMS (Dongbu cytoplasmic and genic male-sterility) cytoplasm and its nuclear restorer-of-fertility locus (Rfd1) with a linked molecular marker (A137) have been reported in radish (Raphanus sativus L.). To construct a linkage map of the Rfd1 locus, linked amplified fragment length polymorphism (AFLP) markers were screened using bulked segregant analysis. A 220-bp linked AFLP fragment sequence from radish showed homology with an Arabidopsis coding sequence. Using this Arabidopsis gene sequence, a simple PCR marker (A220) was developed. The A137 and A220 markers flanked the Rfd1 locus. Two homologous Arabidopsis genes with both marker sequences were positioned on Arabidopsis chromosome 3 with an interval of 2.4 Mb. To integrate the Rfd1 locus into a previously reported expressed sequence tag (EST)-simple sequence repeat (SSR) linkage map, the radish EST sequences located in three syntenic blocks within the 2.4-Mb interval were used to develop single nucleotide polymorphism (SNP) markers for tagging each block. The SNP marker in linkage group 2 co-segregated with male fertility in an F2 population. Using radish ESTs positioned in linkage group 2, five intron length polymorphism (ILP) markers and one cleaved amplified polymorphic sequence (CAPS) marker were developed and used to construct a linkage map of the Rfd1 locus. Two closely-linked markers delimited the Rfd1 locus within a 985-kb interval of Arabidopsis chromosome 3. Synteny between the radish and Arabidopsis genomes in the 985-kbp interval were used to develop three ILP and three CAPS markers. Two ILP markers further delimited the Rfd1 locus to a 220-kb interval of Arabidopsis chromosome 3.
( Bongju Kim ),( Youngcho Cho ),( Sunggil Kim ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.1
To introduce downy mildew resistance from a yellow-colored resistant cultivar, `Santero`, into a yellow breeding line, OT803, the F<sub>1</sub> hybrid was produced by crossing Santero and OT803. The bulb color of the F1 hybrids became light pink, suggesting involvement of complementation between the DFR-A and ANS genes in the onion anthocyanin biosynthesis pathway. Since Santero contained active DFR-A and inactive ANS alleles, OT803 was assumed to harbor active ANS and inactive DFR-A alleles. However, some yellow-colored individuals of OT803 were shown to contain the homozygous genotype of the active DFR-A<sup>R4</sup>-like allele. Sequencing of 4,830-bp full-length sequences of this DFR-A<sup>R4</sup>-like allele revealed that the nucleotide sequences of the DFR-A<sup>R4</sup> and DFR-A<sup>R4</sup>-like alleles were identical except for a single nucleotide deletion in the last exon. This single base-pair deletion resulted in creation of a premature stop codon at 2-bp downstream of the deletion mutation. This new DFR-A mutant allele was designated DFR-A<sup>PS2</sup>. The RT-PCR results showed that transcripts of the DFR-A<sup>PS2</sup> allele were significantly reduced, suggesting involvement of nonsense-mediated mRNA decay (NMD) mechanism. The systematic process consisting of PCR amplification and sequencing of the PCR products was modified to identify the DFR-A<sup>PS2</sup> allele among 16 different DFR-A alleles. No additional accession was found to contain the DFR-A<sup>PS2</sup> allele from 155 diverse onion germplasm, indicating very limited distribution of this new DFR-A<sup>PS2</sup> allele.
Young Cho,Thong Sung Ko,Seung Hee Cha,Dai Eun Sok 생화학분자생물학회 1994 BMB Reports Vol.27 No.4
A native membrane-bound form and a solubilized (purified) form of acetylcholinesterase were prepared from mouse brain, and the effects of nonionic surfactants on both solubilization of the bound enzyme and stabilization of the solubilized enzyme were investigated. For the solubilization of acetylcholinesternse from mouse brain membrane, treatment with surfactants was more effective than hydrolytic enzymes or a high ionic-strength treatment, and Triton X-100 was more efficient than an ionic surfactant. The Triton X-100-solubilized enzyme exhibited a lower K_m value (46.0 μM) than the membrane-bound enzyme (59.5 NM). This K_m value was not changed in the presence of Triton X-100. The Km value of membrane-bound enzyme decreased slightly (13%) to 51.8 μM in the presence of Triton X-100. The solubilized enzyme showed an increased (35%) V_(max) value after exposure to Triton X-100, while the V_(max) value of the membrane-bound enzyme was not significantly affected. Most of the nonionic surfactants tested effectively stabilized the solubilized enzyme. The stabilization effect of Triton X-100 increased in proportion to an increase in concentration of the surfactant, up to its critical micellar concentration. Nonionic surfactants having lower CMC values were generally more effective in the stabilization of the purified enzyme. This suggests that stabilization of solubilized acetylcholinesterase by nonionic surfactants is due to binding of the surfactants to the hydrophobic domain of the enzyme, thus keeping the enzyme in a micellar environment.
Young Cho,Seung Hee Cha,Dai Eun Sok 생화학분자생물학회 1994 BMB Reports Vol.27 No.4
Neurochemical effects of vemaculogen, a secondary fungal (Penicillium Verruculosum) metabolite were studied using rat synaptosomes. To evaluate the presynaptic effects of vemiculogen, high affinity uptake and potassium stimulated release, as well as spontaneous release of ³H-GABA from synaptosome enriched preparntions from the hippocampus, corpus striatum, and cerebrnl cortex were investigated. High affinity uptake of 3H-GABA into hippocampal and striatal synaptosomes decreased by 36.7 and 26.2%, respectively, in the presence of 0.1 mM vezruculogen, while ³H-GABA uptake in cerebrocortical synaptosomes showed no remarkable change. Vemiculogen had no significant effect on the spontaneous or the stimulated release of ³H-GABA from hippocampal, striatal, and cortical synaptosomes. These results suggest that verruculogen exerts presynaptic effects on GABAergic nerve terminals by inhibiting high affinity uptake systems in the hippocampus and striatum.
A Young Cho,Ju Hwan Oh,Hee-Chan Moon,Gum Mo Jung,Young Suk Lee,최영진,선인오,Kwang Young Lee 대한신장학회 2020 Kidney Research and Clinical Practice Vol.39 No.3
Tenofovir disoproxil fumarate (TDF) is recommended as a first-line monotherapy for chronic hepatitis B (CHB) [1]. Although there are cases of acute kidney injury (AKI) caused by TDF [2,3], there has been no report of a case in a patient who underwent renal replacement therapy in Korea. Here, we describe a severe case of TDF-associated AKI that required hemodialysis in a patient with CHB.