Molecular Cloning, Expression and Characterization of Bovine UQCC and Its Association with Body Measurement Traits

Yongfeng Liu,Linsen Zan,Shuanping Zhao,Honggang Huang,Yong Li,Zhonglin Tang,Shulin Yang,Kui Li 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.5

Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis showed that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454). Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing an animal model using cattle to study big animal body type.

Enterprise Computer Network Reliability Analysis

Yongfeng Cui,Wei Liu,Ya Li 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

This paper considers the multi-protocol label switching enterprise computer network routing optimization problem, in the network reliability constraints, by selecting the optimal work and alternate paths, and carries on the bandwidth allocation; network total construction cost is minimal. Due to the total construction cost and network reliability is related to choice of the length of the path, the optimal route network design problem can be decoupled into two sub-problems: cost and reliability related issues, among them, the cost of related sub problem study, Sohn et al. For reliability related problems, reduce the search space understanding, this chapter will work best and standby path search space from a hyper plane reduce to the hyper plane line; Then, reliability related algorithm is proposed to solve the problem. Because of the cost and reliability related sub-problem solutions are obtained based on the same search criterion, so the original problem of optimal work and backup path of solution set is by comparing the two sub problems solution to work out. Finally, related algorithm provides a numerical example to verify the reliability and the validity of the global network routing design method.

Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa

Li, Chunjin,Sun, Yongfeng,Yi, Kangle,Li, Chengjiao,Zhu, Xiaoling,Chen, Lu,Zhou, Xu Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.10

The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation.

Molecular Cloning, Expression and Characterization of Bovine UQCC and Its Association with Body Measurement Traits

Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Huang, Honggang,Li, Yong,Tang, Zhonglin,Yang, Shulin,Li, Kui Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.30 No.5

Ubiquinol-cytochrome c reductase complex chaperone (UQCC) involved in the development and maintenance of bone and cartilage is an important candidate gene for body measurement traits selection through marker-assisted selection (MAS). The expression of UQCC is upregulated in many human and animal models of height as well as other stature indexes. We have cloned the cDNA sequence coding UQCC gene in bovine. Genomic structural analysis indicated that bovine UQCC shares a high similarity with human UQCC. Furthermore, Real-Time PCR analysis showed that the expression of bovine UQCC is remarkably different in diverse tissues, including high level expression in the spleen, heart and windpipe, and relatively low expression in other tissues. We also analyzed allele frequencies in different cattle breeds and an association study on the selected SNPs. SNP DraI A2691T in intron 1 and SNP Bsh1236I A3150G in intron 8 are significantly associated with Body Length (BL), Rump Length (RL), Chest Depth (CD) and Pin Bone Width (PBW). For the A2691T SNP marker, there are significant effects on the RL (p = 0.0001), CD (p = 0.0059) and PBW (p < 0.0001) in 679 individuals; with A3150G SNP marker, there are significant effects on the BL (p = 0.0047) and CD (p = 0.0454). Regarding association analysis of combination of the two SNPs, there are significant effects on the BL (p = 0.0215), CD (p = 0.0282) and PBW (p = 0.0329) in the total population. The results suggest that the UQCC gene is a candidate gene of body measurement traits in bovine reproduction and breeding, and provide data for establishing an animal model using cattle to study big animal body type.

Coordinated Control of Hybrid Energy Storage System in the Microgrid

Hua Li,Yongfeng Ren,Le Li 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.7

Application of Improved BP Neural Network with Correlation Rules in Network Intrusion Detection

Yongfeng Cui,Xiangqian Li Ma,Zhijie Liu 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.4

To detect various network attacks in real time, this paper developed a network intrusion detection system based on artificial neural network. This paper first introduced the recent development of neural network, BP algorithm and structure of a simple perceptron. Then, this paper developed an improved BP neural network algorithm to detect anomaly network traffic with adjusted correlation rules. Finally, the network intrusion system in this paper was tested in a real network situation; the improved BP algorithm neural network with adjusted correlation rules shows a reduction in total error and increment in alarm rate compared to the traditional basic BP algorithm model.

Delamination identification of laminated composite plates using measured mode shapes

Yongfeng Xu,Da-Ming Chen,Weidong Zhu,Guoyi Li,Aditi Chattopadhyay 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.23 No.2

An accurate non-model-based method for delamination identification of laminated composite plates is proposed in this work. A weighted mode shape damage index is formulated using squared weighted difference between a measured mode shape of a composite plate with delamination and one from a polynomial that fits the measured mode shape of the composite plate with a proper order. Weighted mode shape damage indices associated with at least two measured mode shapes of the same mode are synthesized to formulate a synthetic mode shape damage index to exclude some false positive identification results due to measurement noise and error. An auxiliary mode shape damage index is proposed to further assist delamination identification, by which some false negative identification results can be excluded and edges of a delamination area can be accurately and completely identified. Both numerical and experimental examples are presented to investigate effectiveness of the proposed method, and it is shown that edges of a delamination area in composite plates can be accurately and completely identified when measured mode shapes are contaminated by measurement noise and error. In the experimental example, identification results of a composite plate with delamination from the proposed method are validated by its C-scan image.

Stimuli-Responsive Polymers: Fundamental Considerations and Applications

Yongfeng Gao,Menglian Wei,Xue Li,Wenwen Xu,Andrews Ahiabu,Juliana Perdiz,Zining Liu,Michael J. Serpe 한국고분자학회 2017 Macromolecular Research Vol.25 No.6

Stimuli-responsive polymers are capable of changing their chemical and/ or physical properties in response to environmental stimuli. This unique feature has allowed stimuli-responsive polymers to be used in a variety of applications. In this review, we present a basic introduction to the theories that have been developed to describe polymer chains, brushes, and networks. We then detail numerous examples of how stimuli-responsive polymers can be used for sensing and biosensing, drug delivery, and as artificial muscles. While we focus the review on these particular areas, there are numerous other demonstrations of the applications of these fascinating materials, and we are certain that many applications have yet to be discovered.

Seismic Performance of New-type Box Steel Bridge Piers with Embedded Energy-Dissipating Shell Plates under Tri-directional Seismic Coupling Action

Haifeng Li,Xuanneng Gao,Yang Liu,Yongfeng Luo 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.1

Accurate numerical models are necessary to evaluate the seismic performance and load-bearing mechanism of new-type box steel bridge piers with embedded energy-dissipating shell plates under tri-directional seismic coupling action. Numerical simulations of seismic performance under six types of tri-directional seismic coupling action was conducted. The effects of this stress on the seismic performance of the new-type steel bridge piers was evaluated through analysis of damage mode, hysteresis curve, skeleton curve, stiffness and strength degradation characteristic, and energy-dissipating capacity. This study also compared the numerical analysis with experimental results in order to validate the accuracy of the proposed finite element model. Based on this model, the range of relevant parameters expanded and 88 numerical specimens were analysed for seismic performance, producing further information about the influence of thickness and curvature of the embedded shell plate, spacing of transverse stiffening ribs of the shell, axial compression ratio, and slenderness ratio. Results showed that tri-directional seismic coupling action significantly affects the specimen’s deformation capacity; the embedded shell plate effectively improves the piers’ loadcarrying and deformation capacity; and the thickness of the embedded shell plate, width-to-thickness ratio of the wall plate, axial compression ratio, and slenderness ratio significantly affect the seismic performance of the new-type steel bridge piers. To promote the ease of seismic design of new-type box steel bridge piers, this study used theoretical analysis and numerical simulation to calculate equations for the minimum height of the energy-dissipating zone of the bottom embedded shell plate. Finally, formulas were also established to calculate the relevant stability bearing capacity and displacement ductility factor of the new-type steel bridge piers under tri-directional seismic coupling action in order to improve their seismic design.

Expression of Neurotrophin 4 and Its Receptor Tyrosine Kinase B in Reproductive Tissues during the Follicular and Luteal Phases in Cows

Sun, Yongfeng,Li, Chunjin,Sun, Yanling,Chen, Lu,Liu, Zhuo,Ma, Yonghe,Wang, Chunqiang,Zhang, Wei,Zhou, Xu Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.3

The neurotrophins, required for the survival and differentiation of the nervous system, are known to be important for the development of the reproductive tissues. However, the signals initiating the growth of follicles, gamete development, and transport and the development of zygote in the reproductive system of cows remain ambiguous. The purpose of the present study was to identify the transcripts and proteins of Neurotrophin 4 (NT4) and its receptor tyrosine kinase B (TrkB) in bovine reproductive tissues. The transcripts and immunoreactivity of NT4 and TrkB proteins were detected by reverse transcription polymerase chain reaction and western blot analysis. Using immunohistochemistry, the specific immunoreactivity of NT4 and TrkB were detected in the oocytes of primordial follicles and in the growing primary follicles. The NT4 and TrkB immunoreactivity was predominantly observed in granulosa cells, cumulus granulosa cells, cumulus oocyte complexes, theca cells of mature follicles, as well as in the oviduct epithelial cells, uterine gland cell, and epithelium cells of the uterus during the follicular and luteal phases in cows. Expressions of NT4 and TrkB mRNAs were not significantly different among the ovary, oviduct, and uterus of the follicular phase. For the luteal phase, the expression of NT4 mRNA in the ovary was significantly higher than that in the oviduct and uterus, and the expression of TrkB mRNA in the oviduct was significantly higher than that in the ovary and uterus, as determined by fluorescence quantitative reverse transcription polymerase chain reaction. The expression of NT4 mRNA was significantly higher than that of TrkB mRNA in the ovary and uterus, whereas NT4 mRNA expression was lower than that of TrkB mRNA in the oviduct during the luteal phase. The present study hypothesizes that NT4 participates in the regulation of both gonads and extra-gonadal reproductive tissues in cows.