Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia

Zhu, Weidong,Lee, Seok-Woo Korean Academy of Periodontology 2016 Journal of Periodontal & Implant Science Vol.46 No.1

Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis, and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.

A Fusion Model for Securities Analysts' Stock Rating Information Based on the Evidential Reasoning Algorithm under Two-dimensional Progressive Recognition Framework

Weidong Zhu,Yiling Wang,Yong Wu,Yibo Sun 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.7

Securities analysts' forecast information can effectively reduce the uncertainty of information in securities markets, and can also promote effective allocation in capital market. The personality difference of securities analysts will lead to different analysis results. In order to improve the utilization of analysts' forecast information, evidential reasoning algorithm under two-dimensional progressive framework and grouping method for combining evidence were used in this paper to fuse securities analysts' stock rating information. Based on the forecast earnings information and stock rating information of analysts, we constructed a two-dimensional progressive framework, and then fused stock rating information of multiple analysts into one piece of evidence information. Finally, we empirically verified the model in this paper by using Chinese analysts' forecast information. The analysis on the fusion results have shown that: compared to traditional statistic model, the accuracy, certainty and the discrimination of the fusion results in our model have been improved.

Establishment of a Belt and Road Dispute Settlement Mechanism

Weidong Zhu 이준국제법연구원 2019 China and WTO Review Vol.5 No.1

Comments on the Supreme People’s Court’s Interpretation of the New Private International Law ACT in China

ZHU Weidong,GUO Jiong 전북대학교 동북아법연구소 2014 동북아법연구 Vol.8 No.1

A Plea for Unifying or Harmonizing Private International Law in East Asia

Weidong ZHU 한국국제사법학회 2011 國際私法硏究 Vol.- No.17

동아시아에서의 법의 통일과 조화는 근래에 이 지역에서의 지역적 통합이 진전되면서 널리 논의되고 있다. 동아시아에서 국제사법은 먼저 유럽, 미국 그리고 아프리카로부터의 경험에 기초하면서 이 지역에서의 국제사법의 저촉을 고려하면서 통일 및 조화되어야 한다는 것이 필자의 견해이다. 통일되고 조화된 국제사법은 지역적 통합을 증진시키고 내부시장을 창설하 는 데 도움을 줄 것이다. 그리하여 필자는 동아시아에서의 국제사법의 통 일과 조화의 가능성과 접근방법을 논의한다. The unification and harmonization of laws in East Asia is widely discussed in recent years with the development of regional integration in this area. The author proposes that private international law in East Asia should first be unified and harmonized based on the experiences from Europe, America and Africa and taking into account the conflicts of private international law in the region. A unified and harmonized private international law will in turn help enhance the regional integration and create an internal market. Then the author discusses the possibility and approach of unifying and harmonizing private international law in East Asia.

Financing Constraints, Principal-Agent and Chinese Listed Firms’ Output Efficiency

Weidong Zhu,Yiling Wang,Jingyu Liu 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.8

It is shown that output under financing constraints and principal-agent can be modeled as a one-side deviation from a frictionless output level. The effects of financing constraints and principal-agent can be identified and quantified by imposing a distribution assumption on the effects. Panel data on Chinese listed firms between 2008 and 2013 are used in the estimation. It is found that: (1) the output efficiency of Chinese listed firms is significantly affected by financing constraints and principal-agent; (2) the severity of financing constraints and principal-agent of firms in different regions varies; (3) firms’ output efficiency in east China varies greatly, and the mean efficiency is the lowest of the three regions.

Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia

Weidong Zhu,이석우 대한치주과학회 2016 Journal of Periodontal & Implant Science Vol.46 No.1

Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one’s virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis , and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.

Utility and Collaborative Filtering-Based Evaluation Method

Xiaolong Zhu,Weidong Zhu,Shuai Ding 보안공학연구지원센터(IJUNESST) 2013 International Journal of u- and e- Service, Scienc Vol.6 No.4

Perioperative stress prolong post-surgical pain via miR-339-5p targeting oprm1 in the amygdala

Zhu, Yi,Sun, Mei,Liu, Peng,Shao, Weidong,Xiong, Ming,Xu, Bo The Korean Pain Society 2022 The Korean Journal of Pain Vol.35 No.4

Background: The decreased expression of mu-opioid receptors (MOR) in the amygdala may be a key molecular in chronic post-surgical pain (CPSP). It is known that miR-339-5p expression in the amygdala of a stressed rat model was increased. Analyzed by RNAhybrid, miR-339-5p could target opioid receptor mu 1 (oprm1) which codes MOR directly. So, the authors hypothesized that miR-339-5p could regulate the expression of MOR via targeting oprm1 and cause the effects to CPSP. Methods: To simulate perioperative short-term stress, a perioperative stress prolongs incision-induced pain hypersensitivity without changing basal pain perception rat model was built. A pmiR-RB-REPORT^TM dual luciferase assay was taken to verify whether miR-339-5p could act on oprm1 as a target. The serum glucocorticoid level of rats was test. Differential expressions of MOR, GFAP, and pERK1/2 in each group of the rats' amygdala were tested, and the expressions of miR-339-5p in each group of rats' amygdalas were also measured. Results: Perioperative stress prolonged the recovery time of incision pain. The expression of MOR was down-regulated in the amygdala of rats in stress + incision (S + IN) group significantly compared with other groups (P < 0.050). miR-339-5p was up-regulated in the amygdala of rats in group S + IN significantly compared with other groups (P < 0.050). miR-339-5p acts on oprm1 3'UTR and take MOR mRNA as a target. Conclusions: Perioperative stress could increase the expression of miR-339-5p, and miR-339-5p could cause the expression of MOR to decrease via targeting oprm1. This regulatory pathway maybe an important molecular mechanism of CPSP.

Ethylene Production and Expression of Two Ethylene Biosynthetic Genes in Senescing Flowers of Hosta ventricosa

Zhu, Xiaoxian,Hu, Haitao,Guo, Weidong,Chen, Jianhua,Wang, Changchun,Yang, Ling Korean Society of Horticultural Science 2014 원예과학기술지 Vol.32 No.2

Senescence of Hosta ventricosa flowers was firstly characterized as ethylene-sensitive since the deterioration of the tepal was accompanied by increased endogenous ethylene biosynthesis. The full-length cDNAs and DNAs of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) involved in ethylene biosynthesis were cloned from H. ventricosa flowers. The HvACS ORF with 1347 bp and two introns, encoded a polypeptide of 448 amino acids showing 79% homology with that in Musa acuminata. The HvACO ORF contained 957 bp and three introns, encoding a 318-residue polypeptide showing 83% homology with that in Narcissus tazetta. The timing of the induction of HvACS expression was in correspond to the timing of the increase in ethylene production, and that the up-regulation of HvACO transcript was closely correlated with an elevated ethylene production, but underwent a down-regulation in wounded leaves with elevated ethylene emission. The results, together with expression analysis in vegetative tissues, suggested that both HvACS and HvACO were specifically regulated by flower senescence.