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Synthesis and Characterization of Highly Crystalline Anatase Nanowire Arrays
Zhao, Yong-Nan,Lee, U-Hwang,Suh, Myung-Koo,Kwon, Young-Uk Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.9
We developed a novel synthesis strategy of titania nanowire arrays by employing simple hydrothermal reaction and ion-exchange reaction techniques. Hydrothermal reactions of metallic titanium powder with $H_2O_2$ in a 10 M NaOH solution produced a new sodium titanate compound, $Na_2Ti_6O_{13}{\cdot}xH_2O$ (x~4.2), as arrays of nanowires of lengths up to 1 mm. Acid-treatment followed by calcination of this material produced arrays of highly crystalline anatase nanowires as evidenced by x-ray diffraction, Raman spectroscopy, and transmission electron microscopy studies. In both cases of sodium titanate and anatase, the nanowires have exceptionally large aspect ratios of 10,000 or higher, and they form arrays over a large area of $1.5 {\times} 3 cm^2$. Observations on the reaction products with varied conditions indicate that the array formation requires simultaneously controlled formation and crystal growth rates of the $Na_2Ti_6O_{13}{\cdot}xH_2O$ phase.
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Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Zhao, Yongju,Wei, Hong,Wang, Yong,Wang, Lingbin,Yu, Mingju,Fan, Jingsheng,Zheng, Shuangyan,Zhao, Cong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (SMGT) in the goat were optimized. The standard sperm parameters and the ability to bind foreign genes were assessed to select suitable sperm donor bucks. A total of 134 oestrous does were divided into 4 groups and inseminated using different methods and sperm numbers. The does of Groups I to III were inseminated with fresh semen ($1-2\times10^{7}$ and $10^{6}$ sperm) or frozen-thawed semen ($10^{6}$ sperm), respectively, through conventional intra-cervical AI, and the does of Group IV with frozen-thawed semen ($10^{6}$ sperm) through intrauterine AI. Total genomic DNAs were extracted from ear biopsies of the offspring. The presence of $pEGFP-N_{1}$ DNA was screened by PCR and then by Southern blotting analysis. A total of 76 live kids were produced and 8 kids were tested transgene positive on the basis of agarose gel electrophoresis of the PCR-amplified fragment. Southern blotting analysis of the samples showed 5 positive kids. A transgenic ratio of 10.53% was detected using PCR and 6.58% using Southern blotting. The positive kid rate assayed by PCR and Southern blotting of frozen-thawed goat semen was 3.61% and 9.27% higher than that of untreated semen. The results show that transgenic goats can be produced efficiently by the method of artificial insemination using sperm cells to integrate the exogenous DNA and intrauterine insemination allowed low numbers of DNA-transfected spermatozoa to be used, with satisfactory fertility.
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Zhao, Cheng-Xiao,Liu, Ming,Xu, Yong,Yang, Kuo,Wei, Dong,Shi, Xiao-Hong,Yang, Fan,Zhang, Yao-Guang,Wang, Xin,Liang, Si-Ying,Zhao, Fan,Zhang, Yu-Rong,Wang, Na-Na,Chen, Xin,Sun, Liang,Zhu, Xiao-Quan,Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
Background: Evidence supporting an association between the 8q24 rs4242382-A polymorphism and prostate cancer (PCa) risk has been reported in North American and Europe populations, though data from Asian populations remain limited. We therefore investigated this association by clinical detection in China, and meta-analysis in Asian, Caucasian and African-American populations. Materials and Methods: Blood samples and clinical information were collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=335) and from age-matched normal controls (n=347). The 8q24 (rs4242382) gene polymorphism was genotyped by polymerase chain reaction-high-resolution melting analysis. We initially analyzed the associations between the risk allele and PCa and clinical covariates. A meta-analysis was then performed using genotyping data from a total of 1,793 PCa cases and 1,864 controls from our study and previously published studies in American and European populations, to determine the association between PCa and risk genotype. Results: The incidence of the risk allele was higher in PCa cases than controls (0.222 vs 0.140, $P=7.3{\times}10^{-5}$), suggesting that the 8q24 rs4242382-A polymorphism was associated with PCa risk in Chinese men. The genotypes in subjects were in accordance with a dominant genetic model (ORadj=2.03, 95%CI: 1.42-2.91, $Padj=1.1{\times}10^{-4}$). Presence of the risk allele rs4242382-A at 8q24 was also associated with clinical covariates including age at diagnosis ${\geq}65$ years, prostate specific antigen >10 ng/ml, Gleason score <8, tumor stage and aggressive PCa, compared with the non-risk genotype ($P=4.6{\times}10^{-5}-3.0{\times}10^{-2}$). Meta-analysis confirmed the association between 8q24 rs4242382-A polymorphism and PCa risk (OR=1.62, 95%CI: 1.39-1.88, $P=1.0{\times}10^{-5}$) across Asian, Caucasian and African American populations. Conclusions: The replicated data suggest that the 8q24 rs4242382-A variation might be associated with increased PCa susceptibility in Asian, Caucasian and African American populations. These results imply that this polymorphism may be a useful risk biomarker for PCa in multi-ethnic populations.
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Zhao-Yong Zhu,Qing-Ming Shi,Bao-Feng Han,Xian-Feng Wang,Sheng Qiang,Chun-Long Yang 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9
Twenty novel tetramic acid derivatives (E)-3-(1-(alkyloxyamino)ethylidene)-1-alkylpyrrolidine-2,4-diones were synthesized by the reaction of 3-(1-hydroxyethylidene)pyrrolidine-2,4-diones with O-alkyl hydroxylamines. The title compounds were confirmed by IR, 1H NMR, MS and elemental analysis. The structure of compound 6r was further verified by X-ray diffraction crystallography. The bioassays showed that most of the title compounds exhibited noticeable herbicidal and fungicidal activities.
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기획논문 : 중국 서부지역의 부상과 한류 콘텐츠 수출 가능성 고찰 -도시화 과정 중 도시 활성화를 중심으로-
( Yong Sheng Zhao ) 한국문명학회 2013 문명연지 Vol.14 No.2
China have been attracting world’s attention with its rapid economic growth after reform and opening in 1978. Recent years, however, with change of domestic and foreign environment such as inflation of wages and American and European financial crises, the growth is showing signs of slowing down compare to the past. Because of this circumstance, Chinese government is gradually changing its economic growth method from Export-Led style to Urbanization, Expansion of Domestic Demand, Industrial Structure Acceleration and Expansion of Opening Policy-led style. Among these policies, it is expected that Urbanization can lead Chinese economic growth for considerable period of time. As of the urbanization is being progressed in China, this study have considered possibility of cultural export of Korean wave contents which can raise values of Chinese cities, especially for those of western area.
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Member capacity-based progressive collapse analysis of transmission towers under wind load
Yong-Quan Li,Yong Chen,Guohui Shen,Wenjuan Lou,Weijian Zhao,Hao Wang 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.33 No.4
The wind-induced collapse of transmission towers has raised many concerns. Progressive collapse analysis is recognized as a promising method for the assessment of the collapse-resistant capacity of the transmission tower. The finite element model of an actual transmission tower is firstly built for the analysis, in which the dynamic behavior of the member in failure is taken into account to be in accord with the actual tower collapse. The analysis considering the main design load cases is conducted in advance to determine the case under which the tower has the potential to collapse. The incremental dynamic analysis in association with the explicit time integration algorithm is employed to perform a progressive collapse analysis, where the wind loads are simulated by using the linear filtering method, and the developed failure criterion with axial force and bending moment involved is based on the stability bearing capacity of the members. It is found the tower collapse begins with the horizontal bracing member near the waist. Then, the adjacent members, including the leg members, fail sequentially, and the tower collapses eventually with a shear-type failure. The demand to capacity ratio (DCR) in terms of bearing capacity of the member is defined to quantify the structural behavior, the location of the member that has the potential to fail, and when the initial failure occurs are thereby identified. It is concluded that compared to the member capacity-based analysis, the ultimate strain-based analysis, which is most likely to be an inelastic dynamic analysis permitting a large deformation, may overestimate the bearing capacity of the structure in wind-induced collapse.
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Yong Liao,Wen Cao,Kunpeng Zhang,Yang Zhou,Xin Xu,Xiaoling Zhao,Xu Yang,Jitao Wang,Shouwen Zhao,Shiyu Zhang,Longfei Yang,Dengxiang Liu,Yanpeng Tian,Weizhong Wu 한국유전학회 2021 Genes & Genomics Vol.43 No.6
Background lncRNAs–miRNAs–mRNAs networks play an important role in Gastric adenocarcinoma (GA). Identifcation of these networks provide new insight into the role of these RNAs in gastric cancer. Objectives Biological information databases were screened to characterize and examine the regulatory networks and to further investigate the potential prognostic relationship this regulation has in GA. Methods By mining The Cancer Genome Atlas (TCGA) database, we gathered information on GA-related lncRNAs, miRNAs, and mRNAs. We identifed diferentially expressed (DE) lncRNAs, miRNAs, and mRNAs using R software. The lncRNA–miRNA–mRNA interaction network was constructed and subsequent survival examination was performed. Representative genes were selected out using The Biological Networks Gene Ontology plug-in tool on Cytoscape. Additional analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms were used to screen representative genes for functional enrichment. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) were used to identify the expression of fve candidate diferential expressed RNAs. Results Information of samples from 375 cases of gastric cancer and 32 healthy cases (normal tissues) were downloaded from the TCGA database. A total of 1632 DE-mRNAs, 1008 DE-lncRNAs and 104 DE-miRNAs were identifed and screened. Among them, 65 DE-lncRNAs, 10 DE-miRNAs, and 10 DE-mRNAs form lncRNAs–miRNAs–mRNAs regulatory network. Additionally, 10 lncRNAs and 2 mRNAs were associated with the prognosis of GA. Multivariable COX analysis revealed that AC018781.1 and VCAN-AS1 were independent risk factors for GA. GO functional enrichment analysis found DE-mRNA was signifcantly enriched TERM (P<0.05). The KEGG signal regulatory network analysis found 11 signifcantly enrichment networks, the most prevailing was for the AGE-RAGE signaling pathway associated with Diabetic complications. Results of RT-qPCR was consistent with the in silico results. Conclusions The results of the present study represent a view of GA from a analysis of lncRNA, miRNA and mRNA. The network of lncRNA–miRNA–mRNA interactions revealed here may potentially further experimental studies and may help biomarker development for GA.
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Zhao, Dan,Lee, Illhwan,Park, Jae Yong,Cho, Sang-Hwan,Choi, Chung Sock,Song, Seung-Yong,Kim, Jong Kyu,Lee, Jong-Lam The Electrochemical Society 2016 ECS journal of solid state science and technology Vol.5 No.2
<P>Nano- or micro-structured glass substrates have been widely used for displays, but have a significant drawback called haze that blurs the images. To eliminate haze while maintaining high total optical transmittance (T-total), the optimized design of textured structures on single- and double-side glass substrates was investigated using a rigorous coupled-wave analysis method. With optimized truncated cone-shaped nanostructures having period (Lambda) = 200 nm, top-diameter to bottom-diameter ratio (R) = 0.4 and height (h) >= 150 nm on both sides of glass substrates, we achieved a haze-free substrate with an average T-total = 99.5% in the wavelength (lambda) range 300-800 nm. (C) 2015 The Electrochemical Society. All rights reserved.</P>
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Yong Yan,Shasha Li,Yufeng Ou,Yaxin Ji,Zhou Yu,Lian Liu,Chuanpeng Yan,Yong Zhang,Yong Zhao 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.6
Crystalline In2Se3 films were fabricated by magnetron sputtering from a sintered In2Se3-compound target and the effects of the deposition parameters, including the working pressure and deposition time, on the phase composition, structure, morphology, and optical properties were clarified. Single-phase κ-In2Se3 was prepared at 4.0 Pa, but γ-In2Se3 was recognized when the working pressure was lower than 4.0 Pa. The optical transmittance of the films decreased to 45% and the optical band gap varied from 2.9 to 2.0 eV with increasing film thickness from 80 to 967 nm. Metal-semiconductor-metal (MSM) photodetectors based on γ-In2Se3 thin films with various thicknesses were also fabricated. The result of photosensitivity research on such MSM photodetectors suggests that it may be impossible to fabricate wide-absorption-range MSM devices by just using a single material (γ-In2Se3) because of spatial potential fluctuations in the layers.
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